ABSTRACT
Objective To evaluate the effect of lentinan polysaccharide (LPS) on Alzheimer's disease (AD) rats, and providing a new idea for prevention and treatment of Alzheimer's disease (AD).Methods 96 SPF rats were randomly divided into control group, model group, LPS low dose-treated group, LPS middle dose-treated group, LPS high dose-treated group and positive control group(piracetam treated), each had 16 rats.The model of AD was induced by injection of Aβ25-35(80 pmol/μL), rats in four treatment groups were treated with LPS (200 mg/kg), LPS (400 mg/kg), LPS (600 mg/kg) and piracetam(600 mg/kg) orally once a day for 60 days while control group and model group were given equal-dose saline.After treatment, the ability of navigation and memory were evaluated by water maze test (MWM), and then rats in all groups were sacrificed to obtain the hippocampus.HE staining was used to observe the histopathological changes, the expressions of TACE,Aβ,BACE1 and PP2A protein in hippocampus were detected by Western blot.Results Compared with the model group, high dose LPS could significantly improve the ability of learning and memory (P<0.01) and improve the ability of locating navigation (P<0.01).HE staining results showed that LPS treatment could repair the rat hippocampus injury induced by Aβ25-35 .Western blot analysis showed that the levels of Aβ, and BACE1 were down-regulated in LPS group compared with the model group, while the protein level of PP2A and TACE was up-regulated.Conclusion LPS has a protective effect on Aβ25 -35 induced AD model, and it is expected to be a strategy for treatment of AD .
ABSTRACT
Objective To explore the effect of compound Chinese medicine lotion in the preparation room of the Third Affiliated Hospital of Jinzhou Medical University on the patients with flat wart.Methods Confocal laser scanning microscopy and clinical diagnosis were used to collect 100 cases of flat warts from March 2013 to March 2015.The patients were randomly divided into the treatment group ( Chinese compound lotion) and the control group ( tretinoin cream) with 50 cases in each group.Self-made compound Chinese medicine lotion or Victoria A acid cream for 20 days, after the treatment, the curative effect and relapse rate of the two groups were compared.Results The total effective rate of the treatment group (94.0%) was significantly higher than the control group (76.0%), the difference was statistically significant (P<0.05).The relapse rate in the treatment group was 4.3%, significantly lower than that in the control group the rate was 28.9%, the difference was statistically significant (P<0.05).Conclusions Self-made Chinese compound lotion on flat wart patients have a good effect, and its curative effect on verruca plana is better than tretinoin.