ABSTRACT
Objective:To identify the core genes related to the disease severity of respiratory syncytial virus (RSV) bronchiolitis in children using RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA), aiming to provide reference for predicting the condition of RSV infection.Methods:Twenty-two patients admitted to the Second Affiliated Hospital of Wenzhou Medical University with RSV bronchiolitis from October 1, 2019 to February 29, 2020 were enrolled as the case group. They were divided into three groups based on the severity of the disease: mild group, moderate group and severe group. Twenty-two healthy children were selected as the control group. Total RNA was extracted from whole blood leukocytes and analyzed by RNA-seq to compare the differentially expressed genes (DEGs) between children with RSV bronchiolitis and healthy children. The gene co-expression modules related to disease severity and biological indicators for disease severity assessment were identified.Results:The median age of the 22 patients (19 males and 3 females) was 3 months. The median age of the 22 healthy children (14 males and 8 females) was 4 months. There was no significant difference in age or gender between the two groups. There were 8 cases in the mild group, 7 cases in the moderate group and 7 cases in the severe group. Through significance analysis, 416 DEGs were found in the mild group, 586 in the moderate group and 846 in the severe group. According to WGCNA analysis, 10 co-expression modules were found, among which brown module ( r=0.62, P<0.001) was significantly correlated with disease severity. The protein-protein interaction network of DEGs in brown module was constructed and the top 30 core genes were selected according to the connectivity of gene nodes, among which the genes with high correlation were RBX1 and PSMA7. The expression of RBX1 and PSMA7 genes was up-regulated in the severe group, but their expression in the mild and moderate groups was not significantly different from that in the control group. Conclusions:RBX1 and PSMA7 genes might be biological predictors of disease severity in RSV bronchiolitis.
ABSTRACT
<p><b>OBJECTIVE</b>To compare the effects of moxibustion combined with highly active antiretroviral therapy (HAART) and simple HAART for human immunodeficiency virus (HIV) infected patients.</p><p><b>METHODS</b>A total of 100 patients with HIV receiving HAART were randomized into an observation group and a control group, 50 cases in each one. In the observation group, moxibustion was used at Zusanli (ST 36), Guanyuan (CV 4) and Sanyinjiao (SP 6), etc. combined with HAART of zidovudine, lamivudine, nevirapine and efavirenzone, etc. Simple HAART was used in the control group. The patients were observed for 18 months. The indexes were CD, CD/CD, interleukin 2 (IL-2), interleukin 7 (IL-7), the incidence of side effects and the score of quality of life.</p><p><b>RESULTS</b>After treatment, CD, CD/CD, serum IL-2 and the scores of quality of life (physiological, psychological, social relation fields and comprehensive score) increased and serum IL-7 decreased compared with those before treatment in the two groups (<0.01,<0.05), with better results except CDin the observation group (<0.01,<0.05). The incidences of gastrointestinal side effects and total side effects in the observation group were lower than those in the control group (14% (7/50) vs 32% (16/50), 58% (29/50) vs 80% (40/50), both<0.05).</p><p><b>CONCLUSION</b>Moxibustion combined with HAART for HIV infected patients could reduce the incidence of side effects, improve medication compliance, CD/CD, IL-2, IL-7 and the quality of life.</p>
ABSTRACT
Objective To investigate the sedation values of dexmedetomidine combined with midazolam in appendectomy.Methods 90 patients with acute appendicitis were studied prospectively.According to the principle of completely random digital table,all patients were randomly signed into the dexmedetomidine combined with midazolam group,single midazolam group or single dexmedetomidine group.The hemodynamic changes in the three groups were compared.Results There were no significant differences in the three groups in heart rate and mean arterial pressure at t0 (P >0.05).When compared with the single midazolam group or dexmedetomidine group,patients in dexmedeto-midine group got a significant reduction in heart rate at t1 [(79.73 ±10.36)beat/min vs.(93.30 ±19.32)beat/min and (90.85 ±18.23 )beat/min,F =5.796,P =0.004];at t3 [(79.51 ±11.21 )beat/min vs.(87.53 ± 18.35)beat/min and (95.03 ±14.64)beat/min,F =8.006,P =0.001];and at t5 [(79.73 ±12.24)beat/min vs. (91.80 ±17.71)beat/min and (89.62 ±14.26)beat/min,F =5.584,P =0.005];a reduction in heart rate at t2 and t4,though there was no statistical difference (P >0.05);a significant reduction in mean arterial pressure at t1, t2,t3,t4 and t5 (F =8.444,6.591,8.165,17.121 and 6.977,P =0.000,0.002,0.001,0.000 and 6.977);a sig-nificant reduction in visual analogue scales [(3.40 ±1.28 )points vs.(4.50 ±1.81 )points and (4.37 ± 1.71)points,F =4.131,P <0.05 ];a significant reduction in operation duration [(111.17 ±15.77 )min vs. (129.33 ±13.98)min and (132.50 ±14.67)min,F =0.000,P <0.05];and a significant reduction in bleeding vol-ume [(202.47 ±61.07 )mL vs.(246.87 ±52.06 )mL and (241.93 ±58.71 )mL,F =0.006,P <0.05 ]. Conclusion Dexmedetomidine combined with midazolam contributed to maintaining the hemodynamic stable and reducing the operation duration,intra -operation bleeding volume and visual analogue scales.
ABSTRACT
AIM:To investigate the inhibitory effect of dihydro-?-erythroidine on nicotine induced-current in cultured superior cervical ganglion of neonatal rats.METHODS:Pneumatic pressure administration of drug and whole-cell recording techniques were performed to compare induced-current amplitude.RESULTS:Dihydro-?-erythroidine competitively antagonized nicotinic effect,and EC50 was about 0.015mmol/L.CONCLUSION:Dihydro-?-erythroidine is a competitive antagonist of nicotinic receptors in sympathetic neurons.
ABSTRACT
AIM: To study and evaluate the changes of two main kinds of voltage-gated K+ currents in human atrial fibrillation (AF) and to discuss the role of these changes in the atrial electrical remodeling (AER) caused by AF. METHODS: Specimens of human atrial appendage were obtained from 36 RHD patients (18 with chronic AF and 18 without AF). Single atrial myocytes were acutely dissociated by tissue chunk enzymatic digestion. I_~to1 and I_~Kur in the two groups were measured respectively with the patch-clamp technique in a whole-cell configuration and the I-V curves were compared. RESULTS: I_~to1 and I_~Kur amplitudes in AF groups were significantly reduced and the current densities of both I_~to1 and I_~Kur in AF patients were lower than those in NAF patients. CONCLUSION: The reduction of I_~to1 and I_~Kur may be related to changes in atrial conduction, refractory period and may constitute two main parts of the major mechanisms in the AER of chronic AF. Whether exists a relation between changes of the above K+ currents and that of other ionic currents and the AF initiation and perpetuation deserves further investigation. [