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OBJECTIVE@#To apply trifocal distraction osteogenesis in canine model of skull segmental defects and to provide reference for clinical treatment.@*METHODS@#Six labrador dogs were selected in this study and divided into observation group and control group randomly. Each group contained 3 dogs. Skull segmental defects models were established by surgery, and dogs in bservation group received trifocal distraction osteogenesis treatment. Bone density was observed and compared between two groups during treatment.@*RESULTS@#There were no significant difference in bone density between two groups on th 1st day (P>0.05). The bone density of observation group on the 30th day, and 60th day were higher than that of control group (P<0.01).@*CONCLUSIONS@#Trifocal distraction osteogenesis has significant clinical effect, and it would be widely used in clinical treatment.
Subject(s)
Animals , Dogs , Bone Diseases , Therapeutics , Disease Models, Animal , Osteogenesis, Distraction , Methods , Skull , Wounds and Injuries , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To evaluate the clinical effect of cell-assisted lipotransfer (CAL) for breast augmentation.</p><p><b>METHODS</b>18 patients accepted breast augmentation using CAL. 10 patients completed 6-month follow-up and were involved in the study. The adipose tissue was harvested from patients' thighs, flanks and lower abdomen with Lipokit. After standing, 250 ml fatty portion and 500 ml fluid portion of suction aspirates were processed according to the procedures reported in reference. Flow-cytometry was used to detect the percentage of adipose-derived stem cells(ADSCs) in distilled stromal vascular fraction (SVF). The differentiation function of cultured cells also was assessed. The breast volume and images were evaluated by using MRI before operation, 3 and 6 months after operation. The breast volume was marked as V0, V3 and V6 respectively. The resorption rate of transplanted adipose tissue for each breast was calculated and marked as R3 and R6.</p><p><b>RESULTS</b>Averagely, the percentage of ADSCs in freshly distilled SVF was 41.67%. The in-vitro cultured cell grew well and could differentiate into fat, bone and cartilage. Statistics showed that V0, V3 and V6 was (416.19 +/- 40.43) ml, (551.72 +/- 59.86) ml and (538.81 +/- 68.35) ml respectively. R3 and R6 was (51.20 +/- 11.96)% and (54.22 +/- 12.73)%. There was significant difference between V3 and V0 (P < 0.05), V6 and V0. However, no significant difference was showed between V3 and V6 or R3 and R6. In addition, no cyst or calcification was seen in all MRI images.</p><p><b>CONCLUSIONS</b>In process of breast augmentation using CAL, the distilled SVF contains 41.67% ADSCs which have adipogenic, osteogenic and chondrogenic function. Within 3-month post-operation, the breast volume decreases obviously but the volume sustains after that. Compared with the preoperative volume, the 6-month postoperative volume is significantly increased and the breasts' contour is improved greatly. This study indicates that CAL is a safe and effective way for breast augmentation.</p>
Subject(s)
Adult , Female , Humans , Middle Aged , Adipocytes , Cell Biology , Transplantation , Adipose Tissue , Cell Biology , Cell Differentiation , Cells, Cultured , Mammaplasty , Methods , Stem Cell Transplantation , Stromal Cells , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of botulinum toxin type A (Botox A) injection on hypertrophic scar in rabbit ear model.</p><p><b>METHODS</b>The hypertrophic scar model was established in 16 Japanese rabbits' ears. These wounds were divided into two groups as group T (treated with Botox A, n = 48) and group S (not treated, n = 48). The wounds healing times and scar hypertrophy were observed with 8 specimen of normal skin at the rabbit ears as sham group B. HE stain was used to assess the hypertrophic index (HI). The expression of collagen I and III was tested by western-blot. The cell cycle of fibroblasts was studied by flow cytometry.</p><p><b>RESULTS</b>The HI was significantly lower in group T than in group S (P < 0.01). The expression of collagen I and III, as well as the ratio of I to III, was markedly stronger in group S than in group T (P < 0.01). Compared with group T, more fibroblasts were in G2-M in group S and fewer in G0-G1 (P < 0.05).</p><p><b>CONCLUSIONS</b>Local injection of Botox A can inhibit the formation of hypertrophic scar and the activity of fibroblasts in rabbit ear model. It can significantly decrease the expression of collagen I and III in hypertrophic scar, as well as the ratio of collagen I to III. It serves as the basis for the treatment of hypertrophic scar with Botox A.</p>
Subject(s)
Animals , Female , Rabbits , Botulinum Toxins, Type A , Pharmacology , Cells, Cultured , Cicatrix, Hypertrophic , Metabolism , Pathology , Ear , Pathology , Fibroblasts , Injections, Subcutaneous , Wound HealingABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of botulinum toxin type A on the expression of substance P (SP), calcitonin gene-related peptide (CGRP), transforming growth factor beta-1 (TGF-beta1) and alpha smooth muscle actin A (alpha-SMA) in wound healing.</p><p><b>METHODS</b>60 rats were randomly divided into group C (control) group L (low-dose) and group H (high-dose), with 20 rats in each group. The wound-healing model was established by excision of four full-thickness skin (1 cm x 1 cm, around the injection site) on the back of all SD rats on the 7th day after BTA injection. The wound size was measured and the expression of SP, CGRP, TGF-beta1 and alpha-SMA in wound granulation tissue was assayed by immunohistochemical staining and computerized image analysis before operation, and 3 days, 7 days and 14 days after operation.</p><p><b>RESULTS</b>All the wounds healed 14 days after operation. The wound size in L and H group was not significantly different with that in C group on the 3rd day and 7th day after operation. The positive immuno-staining of SP, CGRP, TGF-beta1 and alpha-SMA in group L and H was significantly weaker than those in C group. Meanwhile, the positive immuno-staining of all above substances in H group was weaker than those in L group significantly.</p><p><b>CONCLUSIONS</b>Botulinum toxin type A can decrease the expression of SP, CGRP, TGF-beta1, and alpha-SMA in wound healing in a dose-dependent manner with no effect on the healing time.</p>
Subject(s)
Animals , Male , Rats , Actins , Metabolism , Botulinum Toxins, Type A , Pharmacology , Calcitonin Gene-Related Peptide , Metabolism , Rats, Sprague-Dawley , Skin , Metabolism , Substance P , Metabolism , Transforming Growth Factor beta1 , Metabolism , Wound HealingABSTRACT
<p><b>OBJECTIVE</b>To study the role of focal adhesion kinase (FAK) in the pathogenesis of human hypertrophic scar.</p><p><b>METHODS</b>Human hypertrophic scar fibroblasts (HSFB) were isolated from human hypertrophic scar and cultured in vitro. The cells were then divided into 3 groups as AT group (phosphorothioate FAK ASODN was transfected into the HSFB by liposome), LPC group (liposome only), and LC group (control group, without liposome or ASODN). The FAKmRNA index of HSFB was assessed by polymerase chain reaction method (FQ-PCR). The collagen synthesis of HSFB was assessed by 3H-proline incorporation method.</p><p><b>RESULTS</b>The FAK mRNA index of HSFB in AT group 48 hours after transfection was significantly lower than that in LPC and LC groups (0.043 +/- 0.030, 0.124 +/- 0.070, 0.127 +/- 0.0195, P < 0.05). The 3H-proline incorporation rate in AT group was lower than that in LPC and LC groups (257.0 +/- 15.14, 962.2 +/- 300.5, 930.8 +/- 28.97, P < 0.01).</p><p><b>CONCLUSION</b>The expression of FAK gene and collagen synthesis of the cultured HSFB could be inhibited by FAK ASODN, indicating that FAK played a role in the development of excessive fibrosis of human hypertrophic scar.</p>
Subject(s)
Humans , Cells, Cultured , Cicatrix, Hypertrophic , Genetics , Metabolism , Pathology , Collagen , Fibroblasts , Metabolism , Focal Adhesion Protein-Tyrosine Kinases , Genetics , Metabolism , Oligonucleotides, Antisense , Genetics , Pharmacology , TransfectionABSTRACT
<p><b>OBJECTIVE</b>To study the mechanism of integrin in hypertrophic scar.</p><p><b>METHODS</b>Fibroblasts from 10 samples of human hypertrophic scars was cultured, FQ-PCR assay was applied to detect mRNA expression of alpha-SMA in hypertrophic scar fibroblasts after integrin and FAK antibody blocking.</p><p><b>RESULTS</b>mRNA of alpha-SMA in fibroblasts expressed obviously lower after integrin and FAK antibody blocking than that of control groups ( P < 0.05).</p><p><b>CONCLUSION</b>Through accelerating the synthesis of alpha-SMA, integrin and FAK play an important role in contracture of hypertrophic scar.</p>
Subject(s)
Adult , Humans , Young Adult , Actins , Cells, Cultured , Cicatrix, Hypertrophic , Metabolism , Pathology , Contracture , Metabolism , Pathology , Fibroblasts , Metabolism , Focal Adhesion Kinase 1 , Integrins , RNA, Messenger , MetabolismABSTRACT
Objective To investigate the effects of intense pulsed light(IPL) on the collagen metabolism in human fibroblasts.Methods The callan foreskin fibroblasts were cultured and treated with different wave length and designed dose of IPL irradiation(590-1 200 nm,and 29 J/cm2),while 10 without IPL irradiation as controls.By culturing 1,12,24 and 48 h after the irradiation,the synthesis of collagen was measured by 3H-proline incorporation determination. Results The synthesis of collagen activity increased significantly(P
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Objective To explore the clinical efficacy of intensive pulsed light(IPL) on body hair removal.(Methods A) total of 172 patients who suffered from heavy body hairs were treated by IPL since April 2002.It took 3 to 5 procedures,with the interval of one month. Results After treatment,80 patients(47%) were cured,42(24%) improved,and 50(29%) had no effect. Conclusion The intensive pulsed light system is safe and efficient in hair removal.