ABSTRACT
The timely diagnosis of early gastric cancer is important for guiding the choice of treatment and prolonging the survival of patients. However, current screening of early gastric cancer still relies on endoscopic examination due to the lack of sensitive, accurate, and convenient screening tools. The cost of large-scale screening through gastrointestinal endoscopy is high and many countries and regions cannot afford it. At present, many types of biomarkers have been proved to own the ability to predict and diagnose tumors. Aptamer, as single-stranded oligonucleotides DNA or RNA, can be used to construct sensitive and reliable biosensors based on fluorometry, colorimetric, electrochemistry, etc. through label modification, coupling with various novel materials, etc. Additionally, it is also widely used in the field of precision medicine due to its small molecular weight, high specificity, and natural biological properties. We summarized and concluded aptamer-based gastric cancer diagnostic and therapeutic studies to help researchers quickly get access to tumor-related aptamers screening process, biosensor construction and optimization methods, and therapeutic strategies. This study aimed to provide a reference for the subsequent development of gastric cancer-related aptamers that meet clinical needs and assist in the endoscopic examination, diagnosis, and follow-up.
ABSTRACT
Objective:To explore the relationship between diffusion tensor imaging (DTI) parameter of fractional anisotropy (FA) and recovery of upper-extremity motor function in patients with ischemic stroke. Methods:From January to December, 2019, 20 ischemic stroke patients accepted routine medication and rehabilitation for three weeks. They received DTI examination and were measured FA of the infarct and the corresponding area on the contralateral side, the cerebral foot and the posterior limb of internal capsule of affected and unaffected sides, while the bilateral FA ratio (rFA) of them were calculated, before and after treatment. Meanwhile, all the patients were assessed with Fugl-Meyer Assessment-Upper Extremity (FMA-UE). Results:The FMA-UE score improved after treatment (t = 9.074, P < 0.001), while FA and rFA increased in infarct area (t > 14.519, P < 0.001). The difference before and after treatment of FA and rFA in all of the areas positively correlated with that of FMA-UE scores (r = 0.445~0.565, P < 0.05), which was the most in posterior limb of internal capsule of the affected side. Conclusion:FA of DTI alters with the recovery of motor function of the upper extremity after ischemic stroke, especially in the posterior limb of internal capsule.
ABSTRACT
<p><b>OBJECTIVE</b>To predict the exported proteins of the novel bacterium Phenylobacterium zucineum HLK1(T) using genome-wide computational identification by searching the export signals including N-terminal signal peptides and alpha-transmembrane helices.</p><p><b>METHODS</b>The computational identification of signal sequences was based on a consensus between multiple predictive tools, including SignalP V3.0, LipoP V1.0, Phobius and TMHMM 2.0. Type IV signal peptides and proteins exported via TAT machinery were searched manually based on the conservative motifs. All the predicted proteins were classified according to the Cluster of Orthologous Group (COG) standard.</p><p><b>RESULT</b>In the total 3861 proteins encoded by P. zucineum HLK1(T) 1 378 (35.7%) were predicted to be exported proteins, most of which (totally 735, 19.0% of the proteome and 53.3% of all the exported proteins) were uncleavable transmembrane helices. In addition, 499 type I signal peptides (12.9%, 36.2%), 101 lipoproteins (2.6%, 7.3%) were also identified. Four Type IV signal peptides and 12 TAT proteins were detected as well. According to the COG classification standard, most of these exported proteins were P proteins related to inorganic ion transport and metabolism and S proteins whose functions were unknown.</p><p><b>CONCLUSION</b>The genome of HLK1(T) coded various types of exported proteins which may play an important role in the interaction between P. zucineum and the host cell, and facilitate the strain to invade into the cell.</p>