ABSTRACT
To study the chemical constituents of the fruits of Melia toosendan, three limonoids were isolated and purified by repeated silica gel column chromatography and preparative HPLC from the EtOAc extract of M. toosendan. Their structures were determined by their physico-chemical properties and spectroscopic data (1D-NMR, 2D-NMR) as: 24, 25, 26, 27-tetranorapotirucalla-(apoeupha)-1alpha-tigloyloxy-3alpha, 7alpha-dihydroxyl-12alpha-acetoxyl-14, 20, 22-trien-21, 23-epoxy-6, 28-epoxy (1), nimbolinin B (2), and trichilinin D (3), separately. Compound 1 is a new compound, and compound 2 is obtained from this plant for the first time.
ABSTRACT
<p><b>OBJECTIVE</b>To study the constituents of the whole herbs of Vernonia cinerea by bio-activity guided isolation with PC-12 model.</p><p><b>METHOD</b>The constituents were separated by column chromatography and the structures were elucidated by spectroscopic methods.</p><p><b>RESULT</b>Ten compounds were identified to be (-)-clovane-2,9-diol (1), caryolane-1,9beta-diol (2), apigenin (3), chrysoeriol (4), luteolin (5), thermopsoside (6), luteolin-7-O-beta-D-glucoside (7), quercetin(8), apigenin-4'-O-beta-D-glucoside (9), hyperin (10), beta-amyrin aceate (11), lupeol acetate (12).</p><p><b>CONCLUSION</b>Compounds 1, 2, 6 and 10 were isolated from this genus for the first time.</p>
Subject(s)
Animals , Rats , Drugs, Chinese Herbal , Chemistry , Pharmacology , PC12 Cells , Vernonia , ChemistryABSTRACT
Objective To investigate the chemical constituents in the whole plant of Clerodendrum canescens. Methods The compounds were isolated by column chromatography over silica gel or Sephadex LH-20 gel and their structures were elucidated by chemical and spectroscopic methods. Results Seven triterpenoids were isolated and identified as: lupeol (Ⅰ), ?-amyrin 3-undecanotate (Ⅱ), lupeol acetate (Ⅲ), lupeol 3-palmitate (Ⅳ), melastomic acid (Ⅴ), ?-amyrin acetate (Ⅵ), betulinic acid (Ⅶ), respectively. Conclusion Among those compounds, compound Ⅱ is a new one, while compounds Ⅳ-Ⅵ are isolated from the plants of Clerodendrum Linn. for the first time.
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Objective To study the chemical constituents of Zanthoxylum dimorphophyllum and identify the chemical structures.Methods The compounds were isolated by silica gel,Flash column chromatography and purified by crystallization.Their structures were elucidated by spectral methods.ResultsSeven compounds were isolated and identified.They are 6-(3′,methyl-2′,3′-dihydroxy) butyl-7-methoxyl-8-(3″-methyl-2″-butenyl)-coumarin(Ⅰ),8-(3′-methyl)-2′,3′-butenyl-2′-(1″-hydroxy-1″-methyl)-ethyl-6,7-dihydrofurancoumarin(Ⅱ),6,7,8-trimethoxycoumarin(Ⅲ),scoparone(Ⅳ),umbelliferone(Ⅴ),canthin-6-one(Ⅵ),and syringaresinol(Ⅶ).Conclusion Compound I is a novel compound and all compounds are obtained from Z.dimorphophyllum for the first time.
ABSTRACT
OBJECTIVE: To study the constituents of the subaerial of Saussuare deltoidea (DC.)C. B. Clarke. METHODS: The constituents were separated by column chromatography on silica gel and the structures were elucidated by spectroscopic means. RESULTS: Eight constituents were identified to be Luteolin(Ⅰ), Quercetin(Ⅱ), Quercetin 3 0 ? L rhamnopyranoside(Ⅲ), rutin(Ⅳ), Lupeol acetate(Ⅴ), Lupeol(Ⅵ), ? sitosterol (Ⅶ), and Daucosterol(Ⅷ).CONCLUSION: Compounds Ⅰ Ⅷ were obtained from S.deltoidea for the first time.