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Objective:To investigatethe maternal and infant adverse outcomes of group B streptococcus (GBS) colonization during the late pregnancy.Methods:A total of 567 pregnant women who underwent obstetrics and gynecology examination in Yuyao People′s Hospital from December 2018 to June 2019 were selected for the study. Internal vaginal secretions of all the pregnant women were extracted from the lower 1/3 of the vagina for bacterial culture, and GBS culture and screening were performed on pregnant women. According to the results of GBS screening, pregnant women were divided into GBS negative group (8 cases) and GBS positive group (559 cases). The general condition and pregnancy outcomes of pregnant women were compared between the two groups, and the correlation between pregnancy outcomes and streptococcus colonization was analyzed.Results:There were no statistically significant differences in age, gestational weeks, number of births, number of pregnancies, history of abortion, residence area and ethnicity between the two groups ( P>0.05). The incidence of premature rupture of membranes and fetal distress in GBS positive group were higher than those in GBS negative group:5/8 vs. 7.69%(43/559), 3/8 vs. 5.01%(28/559), and the differences were statistically significant ( P<0.05). Spearman single factor correlation analysis showed that GBS colonization in pregnant women were positively correlated with premature rupture of membranes and fetal distress ( r = 0.632, 0.573, P<0.05). Conclusions:GBS colonization in late pregnancy is closely related to pregnancy outcomes, and strengthening GBS screening has great significance to reduce adverse pregnancy outcomes.
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The apoptosisˉrelated gene is an important gene in the human body, and maintains the apoptosis process through the synergistic action of antiˉapoptosis and proˉapoptosis. Abnormal expressions of apoptosisˉrelated genes and their proteins play important roles in the development of diffuse large Bˉcell lymphoma (DLBCL). Recent studies have shown that targeting apoptosisˉrelated genes have potentials in the treatment of lymphoma, and the treatment of Bclˉ2 inhibitors and Bax activators combined with chemotherapy has attracted much attention. This article reviews the progress of apoptosisˉrelated genes and DLBCL.
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Objective To study the homology relationship among clinically isolated imipenem-resistant klebsiella pneumoniae strains and possible transmission route of drug resistant strains to provide a basis for blocking the transmission of this kind of bacteria in clinc.Methods Clinical samples collection and bacterial culture were routinely conducted,meanwhile the hand surface of ICU medical staffs and the surfaces of patien's bedside objects were performed the sample collection for conducting the bacterial count and culture.The bacterial identification and drug susceptibility test were performed by the VITEK-2 COMPACT system.The bacterial homology detection adopted the ERIC-rep-PCR method.Results Totally 642 strains of imipenem-resistant Klebsiella pneumoniae were clinically isolated from January 2011 to December 2013.Among them,389 strains(60.59％) were derived from ICU.72 strains (11.21％) fsrom the respiratory department,53 strains (8.26％) from the neurology department,41 strains (6.39 ％) from the cardiovascular department,31 strains (4.83％) from the surgical department,11 strains (1.71％) from the gastroenterology department,5 strains (0.78 ％) from the renal department,17 strains (2.65 ％) from the emergency department and 23 strains(3.58％) from the other departments.One strain of imipenem-resistant Klebsiella pneumoniae was isolated from the IGU object surface.Among 642 clinical bacterial strains,there were 8 main clones,these 8 clone strains added up to 394 strains,which accounted for 61.37％ of whole bacterial strains,the other 248 strains were single clones.Conclusion The monoclonal prevalence of imipenem-resistant klebsiella pneumonia in this hospital actually exists.The contact transmission may be the possible transmission route of this kind of infection.
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Objective To verify the accuracy,repeatability and consistency with contracting reagent of the high-sensitive C reac-tive protein(hs-CRP)test by using the independently developed reagents.Methods 150 samples were collected,including 130 ser-um samples and 20 plasma samples.4 times of the relative difference of mean value was used as the detection limits,and the regres-sion analysis was performed after excluding 5 sample outliers,then calculated the expected bias at medical decision level with the 95% confidence interval,in order to judge whether the bias was within the allowable range.Results The recovery rate of hs-CRP test reagent were within the allowable range which was 90% -110%.The hs-CRP testing results of plasma and serum samples from the same source were consistent,and the expected bias of medical decision level was in the permissible range.Conclusion The developed reagent used in hs-CRP test have good accuracy,repeatability,and highly consistency with control reagent.
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Objective To study changes of glial fibrillary acidic protein (GFAP) expression and neural apoptosis in rat hippocampus and cortex of cesarean delivered offspring.Methods Thirty-eight pregnant SD rats were randomly allocated into 2 groups: 19 rats in vaginal delivery (VD) and 19 rats in cesarean section (CS).Forty-eight fetuses born by VD were kept intact, 40 fetuses were delivered by CS on day 21 of gestation.The fetal brain tissues were taken out on postnatal day 30 and 115, the expression profiles of GFAP in hippocampus and cortex were measured by immunohistochemical staining and western blot Apoptotic cells were detected using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining.Results (1) The expression profiles of GFAP: on postnatal day 115, the mean number of GFAP-immunoreactive astrocytes of hippocampus 29.7 ± 10.9 in VD group was significantly lower than 36.2 ± 2.8 in CS group ( P ＜ 0.05 ).The average GFAP-positive cells in the cortex of frontal lobe of 23.2 ±4.6 in VD group was significantly lower than 36.8 ± 5.9 in CS group (P ＜0.01 ).Likewise, on postnatal day 30, the mean number of GFAP-immunoreactive astrocytes of frontal cortex of 27.8 ± 6.0 in VD group was remarkably lower than 39.4 ± 4.5 in CS group ( P ＜ 0.01 ).The average GFAP-positive cells in the hippocampus of 31.5 ±3.5 in VD group were not significantly lower than 37.2 ±7.0 in CS group ( P ＞0.05 ).The expression of GFAP was detected in hippocampus and frontal cortex by western blot, however,there was no significant different expression of GFAP between VD group and CS group.(2) Neuronal apoptosis: TUNEL staining results indicated that, on postnatal day 115, fewer apoptotic cells scattered in offspring hippocampas subregion were only shown in CS group, never in VD group.No TUNEL positive staining cells were labeled in hippocampal subregion in VD group, therefore significantly lower than that of CS group ( P ＜ 0.05 ).Conclusions There were different influences of cesarean section on GFAP expression in hippocampus or cortex in different developmental stage of offspring Cesarean section might increase GFAP expression in the hippocampus and frontal cortex, even trigger neuronal apoptosis of hippocampus region.
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Objective To investigate the expression of vascular growth factors including vascular endothelial growth factor(VEGF), basic fibroblast growth factor(bFGF) and platelet-derived growth factor (p-DGF) in placentae of pregnancy-induced hypertension(PIH) patients and their significance. Methods Placental tissues of 15 normal gravidas and 19 PIH women were collected. The expression of vascular growth factors were detected by immunohistochemistry (PAP) combined with the analysis of computer microphotography. Results The average grey value difference (GVD) of VEGF was 61.6?3.7 in moderate and severe PIH group, which was significantly lower than that of the normal control group (71.1?5.8) and mild PIH (70.9?8.9, P