Poly-aspartamide-glutamic acid grafted low molecular weight polyethylenimine as a novel non-viral gene vector / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To develop a novel gene delivery vector with poly-aspartamide-glutamic acid and polyethylenimine as the backbone.</p><p><b>METHODS</b>alpha, beta-poly-(N-2-hydroxypropyl)-D, L-aspartamide-glutamic acid (PHPAG) was synthesized and low molecular weight polyethylenimine (PEI 1.8 kDa) was grafted to form PHPAG-PEI 1800. Chemical and biological characterization of the polymer was identified.</p><p><b>RESULT</b>The polymer was confirmed by (1)H-NMR, and the molecular weight was about 1.2 x 10(4). The ability of DNA binding was showed by gel retardation assay at N/P ratio of 3. 5. MTT assay showed that the polymer was non toxic in COS-7 and A293 cell lines. In vitro test demonstrated that it had high transfection efficiency in B16 and Hela cell lines.</p><p><b>CONCLUSION</b>PHPAG-PEI 1800 was successfully synthesized,which might be a potential vector for gene delivery.</p>

Aspirin-PEI-beta-CyD as a novel non-viral vector for gene transfer / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To develop a novel non-viral gene delivery vector based on PEI-beta-CyD as backbone modified with aspirin, and to identify its physicochemical characters.</p><p><b>METHODS</b>1, 1-carbonyldiimidazole (CDI) was used to bind aspirin onto PEI-beta-CyD to form PEI-beta-CyD-ASP. (1)H-NMR, FT-IR, UV and XRD were used to confirm the polymer structure. The ability of condensation was demonstrated by gel retardation assay. MTT assay was used to test the cell viability in B16, Hela and A293 cell lines. Transfection efficiency of the polymer was tested in B16 cells.</p><p><b>RESULT</b>The structure of PEI-beta-CyD-ASP was confirmed by (1)H-NMR, FT-IR, UV and XRD, which efficiently condensed plasmid DNA at the N/P ratio of 4. The copolymer showed low cytotoxicity and high transfection efficiency in B16 cells.</p><p><b>CONCLUSION</b>The synthesized aspirin-PEI-beta-CyD might be a potential gene delivery vector.</p>