ABSTRACT
To study the influence of hypercholesterolemia with caveolin-1 on the plasmalemma of vascular endothelium, we used the methods of immunohistochemistry to detect the dynamic changes of caveolin-1 in cultured ECV-304 cells which were stimulated high cholesterol serum and the arterial endothelium of hypercholesterolemia rats. It is resulted that high cholesteorol level can upregulate the expression of caveolin-1 both in vitro and in vivo. In the initial stage of hypercholesterolemia model, the expression of caveolin-1 increased as the time of high cholesterol level added, but in the later period it was decreased slightly.
Subject(s)
Animals , Female , Humans , Male , Rabbits , Rats , Aorta , Pathology , Caveolin 1 , Caveolins , Genetics , Cells, Cultured , Cholesterol , Blood , Endothelium, Vascular , Cell Biology , Metabolism , Hypercholesterolemia , Metabolism , Rats, Sprague-Dawley , Umbilical Veins , Cell BiologyABSTRACT
AIM: To investigate the role of transferrin/transferrin receptor system in transferrin-bound Yb 2 (Yb 2Tf) uptake by U-87 MG cells and the effect of transferrin-bound and -free Yb 2 on proliferation of U-87 MG cells. METHODS: Cell culture and ICP-MS measurement of Yb 2. RESULTS: Yb 2Tf uptake by U-87 MG cells increased with the concentrations of Yb 2Tf, and reached saturation as the concentration in the incubation medium was raised to about 2 ?mol/L. Also, Yb 2 uptake by the cells increased with increase of the mole ratio (Yb 2: apoTf), reaching a maximum at 1.5 mole ratio. Yb 2Tf in 0.4 ?mol/L significantly inhibited proliferation of U-87 MG cells, however, 10 ?mol/L Yb 3+ had no significant effect on proliferation of the cells. CONCLUSION: The uptake of Yb 2 by U-87 MG cells might be mediated by transferrin/transferrin receptor system. Transferrin-bound but not transferrin-free Yb 2 could significantly inhibit proliferation of U-87 MG cells.