ABSTRACT
Objective To investigate the association factors affecting the course of IVF/ICSI-ET. Methods The clinical data of 348 cycles of IVF/ICSI-ET in patients with different causes of infertility were analyzed retrospectively. Results Ovarian reserve and response to stimulation declined with aging. The ability of bFSH predicting ovarian response was low. E 2 peak concentration, number of big follicle and retrieved oocytes decreased significantly when bFSH concentration evelated. The number of big follicle and retrieved oocytes obviously increased with the E 2 peak concentration increasing. The number of antral follicle and volume of ovary were positive relation with ovarian response. None of the factors above could predict the outcome of IVF indenpedently. Conclusion Age was strongly associated with ovarian reserve and response. The bFSH level was one of the indices reflecting ovarian reserve, and had the limited capability for predicting ovarian response and IVF outcome.E 2 peak concentration could not predict the IVF consequence. The number of total antral follicle and volume of ovary were the most direct index reflecting ovarian response. The conditions of patients should comprehensively be evaluated to raise the pregnant rate of IVF.
ABSTRACT
Objective:To study the relationship between luteinizing hormone(LH) ?-subunit gene Gly102Ser polymorphism and polycystic ovary syndrome and premature ovarian failure. Methods: The LH? gene Gly102Ser polymorphism was analyzed in polycystic ovary syndrome(70 cases),premature ovarian failure(34 cases) and normal group(90 cases) by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Results: The LH? gene allelic frequencies of A,G were 0.100, 0.900;0.079,0.921;0.017,0.983 in PCOS,POF and control groups, respectively. The A allele frequencies of LH? gene in PCOS and POF were higher than those in control groups(? 2=10.872,4.413,P0.05). Conclusion: The LH? gene Gly102Ser polymorphism exists in both PCOS and POF people,which is associated with PCOS but not related to POF,and might be a risk factor for PCOS.