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Objective: To analyze the secular trends of incidence and mortality of ovarian cancer in the Changning district of Shanghai, 1973-2013. Methods: Using the data from Shanghai Cancer Registry, the crude rates of incidence and mortality, and the age-standardized rates (ASRs) by Chinese standard population and Segi’s world standard population were calculated for ovarian cancer in the Changning district of Shanghai, 1973-2013. Joinpoint software was utilized to analyze the secular trends of incidence and mortality, as well as to calculate the annual percent changes (APCs) and average annual percent changes (AAPCs). Age-period-cohort model was performed to further investigate the contributions of age, period and cohort effects on the secular trends of incidence and mortality. Results: Total of 936 new incidence cases and 504 deaths in the Changning district of Shanghai were identified during 1973-2013. The crude incidence rate, age-standardized incidence rates by Chinese standard population and Segi’s world standard population were 8.72/105, 6.32/105 and 6.00/105, respectively. The crude mortality rate, age-standardized mortality rates by Chinese standard population and Segi’s world standard population were 4.70/105, 2.99/105 and 2.92/105, respectively. The crude incidence rate, age-standardized incidence rates by Chinese standard population and Segi’s world standard population of ovarian cancer showed steady rising trends during 1973-2013, by an average of 2.96%, 1.51% and 1.63% per year, respectively. The crude mortality rate increased by an average of 2.53% per year, but the secular trends of age-standardized mortality rates by Chinese standard population and Segi’s world standard population were not significant (both P > 0.05). Age-period-cohort analysis showed that both the incidence and mortality rates of ovarian cancer increased with age (both P 0.05). Conclusion: The crude incidence rate, age-standardized incidence rates by Chinese standard population and Segi’s world standard population, and crude mortality rate of ovarian cancer in the Changning district of Shanghai show steady rising trends during 1973-2013. The secular trends of age-standardized mortality rates by Chinese standard population and Segi’s world standard population are not significant. Both the incidence and mortality rates increased with age, suggesting the elderly women are key population for the prevention of ovarian cancer. ovarian cancer.
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OBJECTIVES@#To provide clues for further study of the relationship between miRNAs and Kawasaki disease (KD) development, and to provide molecular markers for ultimately improve the rate of early diagnosis for KD.@*METHODS@#We collected acute, recovery KD children's plasma and normal samples, then used the miRNAs Assay Chip to screen the differentially expressed miRNAs in the plasma from KD children. Subsequently, miR-455-5p, which had identified via miRNAs assay chip, was validated by quantitative real-time PCR via independent cohort.@*RESULTS@#According to the results of miRNAs Assay chip, we identified a miRNAs panel including 5 miRNAs significantly up-regulated and 5 miRNAs remarkably down-regulated in the plasma from KD children compared to the normal control; miR-455-5p in both of acute and recovery KD children's plasma was remarkably lower than that in the normal control (<0.001, =0.013, respectively), and miR-455-5p was also significantly lower than that in the recovery of KD children (=0.007) by independent cohort validation.@*CONCLUSIONS@#There are significantly differentially expressed circulating miRNAs between the KD children and normal control. We identified 10 miRNAs dysregulation in the KD children's plasma compared with the normal group. Circulating miR-455-5p in both of acute and recovery KD children's plasma is remarkably lower than that in the normal control, and miR-455-5p may considered as a marker to show the recovery process of KD children. Plasma specific circulating miRNAs play an important role in the early diagnosis of KD and become the new molecular marker of KD in the future.
Subject(s)
Child , Humans , Biomarkers , MicroRNAs , Genetics , Mucocutaneous Lymph Node Syndrome , Genetics , Oligonucleotide Array Sequence Analysis , Real-Time Polymerase Chain ReactionABSTRACT
Objective:To investigate association between the single nucleotide polymorphisms of endothelial protein C receptor (EPCR) gene and the risk of Kawasaki disease (KD) in a Chinese children.Methods:A total of 103 KD patients including 23 patients with coronary artery lesions (CAL) and 158 controls were recruited.Seven tagging SNPs (rs6088738,rs2069940,rs2069945,rs2069952,rs867186,rs9574,and rs1415774) of EPCR gene were selected for TaqMan allelic discrimination assay.The plasma soluble EPCR (sEPCR) levels of 53 KD and 52 healthy children were detected by ELISA.Results:We found a significant association between rs2069952,rs9574 or rs1415774 and higher probability for the occurrence of KD but not CAL formation.Interestingly,males with these 3 SNPs and rs2069945 SNPs bore a much greater risk of KD than females.The level of plasma sEPCR in children with KD didnot predict the formation of CAL.However,the allele G of rs867186 in EPCR was associated with the increased level of plasma sEPCR in KD patients.Conclusion:The SNPs of EPCR are associated with KD susceptibility in a Chinese Han children.
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Kawasaki disease (KD) is a systemic vascular inflammatory lesion in children with acute febrile rash illness. The inci-dence rate in recent years is increasing. The traditional treatment in some patients is ineffective, and KD has high incidence of cardiovas-cular complications. Therefore, the selection of an appropriate therapy for KD is an urgent. With the continuous advance of biomedical and pharmacologically research, many biological agents were developed. The traditional treatment has been gradually replaced by the new biological agents which achieved the desired results. Thus this paper reviewed several common biological agents in the clinical treatment of KD.
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Objective To study the feasibility of endoscopically subcutaneous mastectomy and immediate mammary prosthesis reconstruction.Methods From December 2006 to October 2007，9 breast cancer patients underwent endoscopic skin sparing mastectomy and immediate implanting breast reconstruction，with preoperatively performed systemic TE chemotherapy two to six times.Whether or not the nipple-areola complexes were preserved depended on the results of frozen pathological examination.Results Of 9 patients，bilateral skin sparing mastectomy were performed in two patients，and others underwent single lateral masteetomy with the nipple-areola complexes，at the same time sentinel lymph node biopsy was done in 8 cases of all.Combined level Ⅰ and Ⅱ axillary dissections were carried out vvia the sarne incisions underthe axillaries in 7 patients，and 2 patients spared axillary dissections.8 of them got satisfactory results，one patient did not.The postoperation complications included subcutaneous seromas 1 case，epidermic nipple necrosis 1 case and bleeding of skin flap 1 case.No local reeurrence occurred during the follow-up ranged 1～10 months.Conclusion It is technically safe and feasible that endoscopically subcutaneous mastectomy with immediate mammary prosthesis reconstruction.The technique can minimize skin incision With little trauma,and offers a greater esthetic advantage tomostpatients.
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Objective To study the effects of mammalian target of rapamycin(mTOR)transfection on the proliferation of NIH3T3 fibroblasts.Methods The plasmid of pcDNA3-mTOR was transfected into NIH3T3 fibroblasts with electroporation method.The positive cell clones were selected with G418.The stable mRNA and protein expressions of mTOR in the cells were determined by RT-PCR and Western blotting analysis respectively.MTT assay was employed to observe the proliferation of NIH3T3 fibroblasts.Results mTOR mRNA and protein expressions increased obviously in transfected group than that of in control group(P