ABSTRACT
@#The second near-infrared window(NIR-II)fluorescent dye IR-1061 has the disadvantage of poorwater solubility and low quantum yield, which limits its application in biomedicine. We used liposomes as the carrier to increase their water solubility, and co-loaded a near-infrared region(NIR-I)fluorescent dye IR-780 into liposomes to enhance the fluorescence intensity of IR-1061 in NIR-II. Results showed that the liposomes exhibited significantly enhanced characteristic fluorescence under 980 nm laser irradiation. In addition, particle size, appearance, photothermal conversion efficiency, photoacoustic performance and cytotoxicity of liposomes were examined. This study showed that these combined liposomes exhibited potential as a NIR-II fluorescence/photoacoustic bimodal imaging system for the diagnosis and guidance of tumor photothermal therapy.
ABSTRACT
@#The combination of diagnosis and treatment through nanotechnology is conducive to the development of cancer treatment. Fluorescent imaging in the second near-infrared window(NIR-II)developed rapidly in recent years due to its imaging advantages. In this paper, we prepared a novel nano drug system, DOX-IR1061-cationic liposome, in which NIR-II fluorescent probe IR1061 was loaded as imaging agents and doxorubicin was loaded as therapeutic agents. It also explores enhanced cellular uptake and cancer cell inhibition rate through octadecylamine. Our NIR-II performance test on liposomes showed that DOX-IR1061-cationic liposome has NIR-II imaging ability. Analysis of liposome cell uptake behavior and cancer cell inhibition experiments demonstrated that octadecylamine can promote liposome uptake by cells and synergize with DOX to enhance anticancer effects. This suggests that the DOX-IR1061-cationic liposome can be used to achieve imaging and therapy effect with further research value.
ABSTRACT
Objective To evaluate the expressions of CD68 and alpha/beta hydrolase domain-containing protein 5 (ABHD5) in gastric carcinoma and their interrelation,and the relationship with the clinicopathological features of gastric carcinoma.Methods The immunohistochemistry staining was used to determine the expressions of CD68 and ABHD5 in 60 cases of gastric carcinoma and 30 cases of adjacent normal tissues.The relationships between CD68,ABHD5 and clinicopathological features of gastric carcinoma,and the correlation of them were analyzed.Results The expressions of CD68 and ABHD5 in gastric carcinoma were significantly higher than those in adjacent normal tissues,and the positive expression rates were 65.00％ vs.26.67％(x2 =11.779,P =0.001) and 60.00％ vs.30.00％ (x2 =7.200,P =0.007) respectively,with significant differences.The expression of CD68 was related to TNM stage (x2 =7.959,P =0.005),lymph node metastasis (x2 =6.901,P =0.009) and serosal invasion (x2 =5.833,P =0.016),but it was not associated with pathological grade (x2 =0.028,P =0.866),gender (x2 =0.533,P =0.465) and age (x2 =0.060,P =0.807).The expression of ABHD5 was related to pathological grade (x2 =4.318,P =0.038),TNM stage (/x2 =4.051,P =0.044) and serosal invasion (x2 =5.275,P =0.022),but it was not associated with lymph node metastasis (x2 =0.545,P =0.460),gender (x2 =0.191,P =0.662) and age (x2 =1.358,P =0.244).Contingency correlation analysis showed that there was positive correlation between CD68 and ABHD5 in gastric cancer (c =0.328,P =0.010).Conclusion The expressions of CD68 and ABHD5 in gastric carcinoma are higher than those in adjacent normal tissues,which are correlated with the different clinicopathological features of gastric carcinoma.The expression of CD68 is positively correlated with the expression of ABHD5.The combined detection of CD68 and ABHD5 expressions in gastric carcinoma is helpful to evaluate the invasion,metastasis and prognosis of gastric carcinoma.