ABSTRACT
Thermophilic bacteria strain YBJ-1 was isolated from hot spring samples collected from Yangbajing, Tibet. The 16sr DNA sequence of YBJ-1 (1511bp in length) shares 98% identity with that of Thermus scotoductus strain ITI-252T. The full-length ORF of amylase gene of YBJ-1 (amyT) was amplified by PCR technique and cloned into T-vector. The complete sequence of amyT is 1767bp in length, coding for 588 amino acids. The deduced amino acids share 99% similarity with alpha-cyclodextrinse of Bacillus sterothermophilus, 96% with maltogenic amylse of Thermus. sp IM6501, and 81% with neopullulanase of Bacillus sterothermophlus.
Subject(s)
Amylases , Genetics , Bacterial Proteins , Genetics , Cloning, Molecular , Culture Media , Open Reading Frames , Genetics , Thermus , GeneticsABSTRACT
A bacteria strain DY3 with high endoglucanse activity was isolated from deep sea sediment sample ES0109. The 16S rDNA sequence of DY3 exhibits identity of 99% with those of the same genus bacteria Pseudoalteromonas citrea and Pseudoalteromonas elyakovii . The celX gene of DY3 obtained by PCR method is 1479bp in length and encodes a protein of 492 amino acids. The protein encoded by celX gene exhibits 95% sequence identity with endoglucanase CelG from Pseudoalteromonas haloplanktis. There are two modules in the deduced amino acids sequence, a catalytic domain of glycosyl hydrolases family 5 at the N terminal and a carbohydrate binding domain at the C terminal which was linked to catalytic domain by a short linker. The optimal temperature of CelX is 40 degrees C and the optimal pH was between 6 and 7.
Subject(s)
Amino Acid Sequence , Bacterial Proteins , Genetics , Cellulase , Genetics , Cloning, Molecular , Gene Expression Regulation, Enzymologic , Molecular Sequence Data , Pseudoalteromonas , Genetics , Sequence AlignmentABSTRACT
ObjectiveTo explore the renal functions of vasopressin-deficient Brattleboro(BB) rats by using different concentrated Gadolinium-diethylenetetramine pentaacetic acid(Gd-DTPA) dynamic enhanced magnetic resonance imaging(MRI).MethodsThe study included 14 BB rats(male rats of 3 month-old) and 14 normal male Wistar rats used as control group.Dynamic MRI was performed by using either a low dosage(0.05 mmol/kg) or a high dosage of Gd-DTPA(0.5 mmol/kg).Data of 0-60 min renal cortex,medulla and pelvic were obtained after using contrast medium.MRI of kidneys at different time was analyzed and the mean relative signal intensity(RSI) was measured.Then the RSI curves of different groups were marked.Data of each group were caculated separately by SPSS 11.0 software.ResultsThe findings demonstrated that RSI curves of the vasopressin-deficient kidneys showed different patterns as compared with those of the control group(P