Liquid resuscitation combined with hydrogen inhalation reduce acute kidney injury during septic shock in rats / 中国病理生理杂志

AIM:To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2％ hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide (LPS) in rats.METHODS:Male Wistar rats were randomly divided into 4 groups (15 rats per group):control group,septic shock group,septic shock with early fluid resuscitation group (fluid group) and septic shock with early fluid resuscitation plus 2％ hydrogen inhalation group (fluid + H2 group).The rats were ventilated,and a 2％ hydrogen mixture was used in fluid + H2 group.LPS (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in fluid group and fluid + H2 group.RESULTS:Fluid resuscitation with 2％ hydrogen inhalation decreased the levels of serum creatinine,blood urea nitrogen and neutrophil gelatinase-associated lipocalin.It also reduced oxidative stress injury and decreased renal tumor necrosis factor-α and interleukin-6 levels compared with fluid resuscitation alone.CONCLUSION:Early fluid resuscitation plus 2％ hydrogen inhalation provided more protection against acute kidney injury during septic shock.

Liquid resuscitation combined with hydrogen inhalation reduce acute kidney injury during septic shock in rats / 中国病理生理杂志

AIM:To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2％ hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide (LPS) in rats.METHODS:Male Wistar rats were randomly divided into 4 groups (15 rats per group):control group,septic shock group,septic shock with early fluid resuscitation group (fluid group) and septic shock with early fluid resuscitation plus 2％ hydrogen inhalation group (fluid + H2 group).The rats were ventilated,and a 2％ hydrogen mixture was used in fluid + H2 group.LPS (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in fluid group and fluid + H2 group.RESULTS:Fluid resuscitation with 2％ hydrogen inhalation decreased the levels of serum creatinine,blood urea nitrogen and neutrophil gelatinase-associated lipocalin.It also reduced oxidative stress injury and decreased renal tumor necrosis factor-α and interleukin-6 levels compared with fluid resuscitation alone.CONCLUSION:Early fluid resuscitation plus 2％ hydrogen inhalation provided more protection against acute kidney injury during septic shock.