ABSTRACT
Background: preeclampsia [PE] is a serious complication of pregnancy with hallmarks of incomplete placentation, placental ischemia and endothelial dysfunction. Imbalance between vascular endothelial growth factor [VEGF], placenta growth factor [PlGF] and their receptors play important role in pathophysiology of PE
Objective: this study was aimed to asses PlGF mRNA expression in placenta of women affected with PE
Material and Methods: in this cross-sectional study, expression of PlGF mRNA was evaluated in 26 mild PE cases, 15 severe preeclamptic women and 20 normotensive controls. Patients were sub-classified as early onset PE [9] and late onset [32]. After RNA extraction, PlGF expression was quantified with qRT-PCR
Results: the results of PlGF mRNA expression between mild-severe, and early-late onset PE patients showed no statistically significant difference compared with the control group [p=0.661, p=0.205 respectively]
Conclusion: despite we found no distinct differential expression of PlGF mRNA in placental tissue of PE patients compared with control women, but according to decreased level of this angiogenic factor in PE even before clinical onset of the disease, determining molecular mechanisms related to reduced secretion of PlGF into the maternal circulation may be useful for future therapeutics
ABSTRACT
Background: The use of biomarkers for diagnosis of Preeclampsia [PE], a life-threatening pregnancy disorder, could reduce serious complications of this disease. In this study, we investigated dysregulation of endoglin [Eng] expression and diagnostic accuracy of soluble endoglin [sEng] in PE patients
Methods: For this case-control study, 26 mild and 15 severe preeclamptic women along with 20 normotensive controls were recruited. The expression level of Eng [the co-receptor of TGF-[31] was evaluated using qRT-PCR
Also, the serum concentration of soluble Eng and expression of membranous Eng were determined by ELISA and immunohistochemistry
Results: A significant up-regulation in Eng mRNA and sEng levels was observed in PE patients versus normal controls. Immunohistochemistry [IHC] showed up-regulation of membranous Eng staining in syncytiotrophoblast and cytotrophoblast cells of PE patients
The serum levels of sEng were significantly increased in all patients [mild, sever, early- and late-onset] as compared to healthy pregnant women [P<0.001]. Receiver-operating characteristic [ROC] curve analysis revealed that sEng had the highest accuracy in distinguishing PE from normal pregnancies with cut-off value of 20.4, sensitivity of 92.1%, specificity of 90%, and area under the curve [AUC] of 0.94 [95% Cl: 0.88-1.00]
Conclusions: Our data showed that the up-regulation of Eng mRNA along with its membranous and soluble form in PE patients leads to defect in angiogenesis pathway. Also, the results of this study revealed sEng potential as a marker for diagnosis of PE and its severity
Subject(s)
Humans , Women , Young Adult , Adult , Endoglin , Biomarkers , Pregnancy Complications , Pregnant Women , RNA, Messenger , Case-Control Studies , IranABSTRACT
Preeclampsia is an importait pregnancy disorder with serious maternal and fetal complications uliich its etiology has not been conlpletely understood yet. Early diagxosis and management of disease could reduce its potential side effects. The vascular endothelial growth Factor [VEGF] family including VEGF-A is the most potent endothelial growth factor which induces angiogenesis and endotheha1 cell proliferation and has basic role in vasculogenesis. VEGF and its tyrosine kinase receptors [Fltl and 1U]R] are major factors for fetal and placental aigiogenic development. Finding mechanisms involved in expression of angiogenic factors may lead to new prognostic and therapeutic points in management of preeclampsia. Recent researches, has shown capability of some antiangiogenic factors as potential candidate to be used as early predictors for preeclampsia. Soluble fins-like tyrosin lcinase-1 [sFltl] is a truncated splice variant of the membrane-bound VEGF receptor Fltl, that is produced by the placenta and it can bind to angiogenic gsowth factors and neutraliz, their effects. It is also observed that the ratio of sFItl to placental growth factor is valuable as prognostic marker. In this review, VEGF family member's role in angiogenesis is evaluated as biomarkers to be used for prediction of preeclampsia.
ABSTRACT
Polycystic ovarian syndrome is one of the most common causes of endocrine disorders and main reason of infertility due to anovulation and recurrent abortions. Progesterone has been shown to have an important role in fertilization of oocyte and fetal implantation. The purpose of this study was to compare the predictive value of progesterone level on IVF success in women with infertility due to tubal factor or PCOS. In a stratified cohort study, we assigned 76 infertile women of 20-38 years old who referred to women hospital into two equal groups with fallopian tube factor infertility and PCOS. We measured the plasma levels of progesterone and estradiol on the day of HCG administration. The patients were divided into two groups based on progesterone level cut off point of 1.2ng/ml. Thereafter the incidence of pregnancy [chemical by beta-HCG measurement and clinical by ultrasonography up to the 6 weeks after fetal transfer] was compared in these groups. Total pregnancy rates were 15.8% in patients with tubal factor infertility and 26.3% in women with PCOS. In women with PCOS, the pregnancy rate was less in patients with progesterone level <1.2 ng/ml. However this difference was not statistically significant. Likewise, we did not observe any significant differences in pregnancy rate in patients with fallopian tube factor infertility. Serum progesterone level on the day of HCG administration is not well predictive of the IVF success in infertile women due to fallopian tube factor or PCOS. To obtain more uniform results, we recommend use of larger samples while the bias variable is taken into account and the ROC curve is used for determination of the unique serum progesterone level