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1.
Article in Chinese | WPRIM | ID: wpr-873237

ABSTRACT

Objective:To investigate the targets and possible mechanism of Didangtang in the treatment of bladder cancer. Method:Based on multiple traditional Chinese medicine and disease databases, the network pharmacology was used to screen potential targets, analyze the biological functions of potential targets, and construct a network of "Chinese medicine-target-path-disease". Bioinformatics analysis was applied in population and gene databases, in order to explore the differential expressions of core targets in tissues, distribution in the population and the correlation with prognosis. The in vitro experiment was used to verify the biological function of Didangtang. The underlying mechanism of Didangtang on the candidate target was detected. Result:A total of 21 core target genes and 16 highly enriched pathways were screened out. A functional network of Didangtang was constructed systematically. At the same time, six targets, namely cadherin 1 (CDH1), CAMP responsive element binding protein 1 (CREB1), colony stimulating factor 2 (CSF2), AP-1 transcription factor (JUN), matrix metalloproteinase 2 (MMP2), and prostaglandin-endoperoxide synthase (PTGS2), were differentially expressed in bladder cancer tissues (P<0.05). Furthermore, JUN and MMP2 were also differentially distributed in population (P<0.05). At the same time, the expression level of JUN was correlated with the prognosis of patients with bladder cancer (P<0.05). The in vitro experiment revealed that Didangtang inhibited the proliferation of bladder cancer cells and decreased the expression of candidate target JUN (P<0.01). Conclusion:Didangtang has the characteristics of multiple targets and multiple pathways in treatment of bladder cancer. It is initially confirmed that Didangtang can affect the expression of target JUN and inhibit the proliferation of bladder cancer, which lays a good foundation for further studies on mechanism.

2.
Article in Chinese | WPRIM | ID: wpr-871687

ABSTRACT

Objective:To compare the clinical effect of anterior mediastinal tumor resection with video assisted thoracoscopic surgery(VATS) through approach of trans-subsagittal incision and trans-intercostal incision.Methods:The clinical data of 118 patients including 67 males and 51 females with anterior mediastinal tumors using VATS resection from January 2013 to October 2020 in Tongren Hospital, Shanghai Jiao Tong University School of Medicine were analyzed retrospectively. According to different incisional approach, these patients were divided into two groups of trans-subsagittal incision group including 45 cases with 25 males and 20 females, and trans-intercostal incision group including 73 cases with 42 males and 31 females. The sternal incision group was treated with the sub xiphoid incision plus the subcostal arch incision and the sternal retractor traction sternum, and the intercostal incision group was treated with the traditional intercostal space incision. Subsagittal incision combined subcostal arch incision and sternum traction with sternal retractor was used in the approach of trans-subsagittal incision group, and the traditional thoracotomy was used in the intercostal incision group. The clinical data of operation time, volume of intraoperative blood loss, rate of transfer to open thoracotomy, duration of postoperative chest tube drainage, visual analogy score(VAS)assessed on the 1st and 3rd days after surgery, hospitalization time, average cost of hospitalization, and incidence of postoperative complications, were compared between the two groups.Results:The operative time, volume of intraoperative blood loss, the number of cases of transfer to open thoracotomy, duration of postoperative chest tube drainage, VAS score of the first and third days after operation, length of hospitalization, average hospitalization cost and the incidence of postoperative complications were lower than those in the transcostal incision group( P<0.05). Conclusion:Compared to the group of trans-intercostal incision VATS resection of anterior mediastinal tumor, the trans-subsagittal incision group has many advantages as follows, shorter operation time, less intraoperative blood loss, no transfer to open thoracotomy, much simpler and safer operative procedure, less body trauma, lighter postoperative pain, less postoperation complications, shorter hospitalization time, less average hospitalization cost, and rapid rehabilitation after surgery.

3.
Article in Chinese | WPRIM | ID: wpr-869269

ABSTRACT

Objective:To compare cerebral vascular hemodynamic indexes (CVHI) and revised Framingham stroke risk profile (FSP) in predicting the risk of stroke among physical examiners.Methods:A total of 1 049 individuals were enrolled from the Health Management Center, Jiangsu Province Hospital during January 2019 and January 2020. Correlation analysis was carried out to confirm the correlations of risk factors of stroke with CVHI accumulative score and FSP. Multivariate logistic regression analysis was performed to evaluate the influence of CVHI classification, hypertension, diabetes and smoking on the 10-year risk of stroke evaluated by FSP.Results:In this study, the number of people whose CVHI accumulative score was less than 75 was 163 (15.54%) and the number of people whose 10-year probabilities of stroke was greater than or equal to 6% was 202 (19.26%). The CVHI accumulative score was negatively related with FSP ( r=-0.284, P<0.001). The result demonstrated that the CVHI classification, hypertension, diabetes and smoking were independent predictors for the 10-year risk of stroke evaluated by FSP. Conclusions:The proportion of individuals with stroke risk in medical examination population evaluated by CVHI is lower than by FSP. Combination of CVHI and FSP can effectively predict the risk of stroke among physical examiners.

4.
Article in Chinese | WPRIM | ID: wpr-864564

ABSTRACT

Objective:To evaluate the effect of standardized training for new nurses in the first year based on Kirkpatrick model as a theoretical framework.Methods:A cross-sectional study was conducted. New nurses' satisfaction with training, theoretical and operational assessment, job performance, core competence, turnover rate and other indicators were used, to evaluate the effect of standardized training for 62 new nurses in the first year.Results:At the reaction level, the overall satisfaction score of new nurses was 4.66±0.47; at the learning level, the theoretical assessment score was 95.32±2.38, the operational assessment score was 97.53±1.07, and the performance score was 97.12±1.64; at the behavioral level 88.45±9.75; and at the result level, there were no complaints and errors among new nurses, and the turnover rate was 1.6% (1/62). The theoretical and operational assessment scores were positively correlated with work performance, with r value of 0.424 and 0.399, respectively, and P = 0.001. The theoretical assessment scores were negatively correlated with the behavioral level, with the r value of -0.264 and P= 0.038. There were no correlation with the operational assessment scores and the behavioral level, with the r value of 0.147 and P value of 0.253. Conclusions:Kirkpatrick model validates the standardized training program for new nurses effectively, and provides scientific basis for improving the training program for new nurses. The results level of the training needs to be evaluated in the long term.

5.
National Journal of Andrology ; (12): 888-894, 2020.
Article in Chinese | WPRIM | ID: wpr-880287

ABSTRACT

Objective@#To investigate the advantages and disadvantages of point electro-cauterization (PEC) and holmium laser cauterization (HLC) in the treatment of post-ejaculation hematuria.@*METHODS@#From January 2015 to December 2018, 73 patients with post-ejaculation hematuria, aged 24-63 (36.8 ± 4.2) years, underwent PEC (n = 35) or HLC (n = 38) after failure to respond to 3 months of conservative treatment. We compared the hospital days, total hospitalization expenses, maximum urinary flow rate (Qmax), average urinary flow rate (Qavg), Hamilton Anxiety Rating Scale (HAMA) score, postoperative duration of hematuria, and recurrence rate at 3 and 6 months after surgery.@*RESULTS@#All the patients experienced first ejaculation but no post-ejaculation hematuria at 1 month after operation. The recurrence rates were lower in the PEC than in the HLC group at 3 months (5.71% vs 2.63%, P > 0.05) and 6 months postoperatively (8.57% vs 5.26%, P > 0.05). Compared with the baseline, the Qmax was decreased from (18.56 ± 2.53) ml/s to (13.68 ± 3.31) ml/s (P < 0.05) and the Qavg from (14.35 ± 2.26) ml/s to (9.69±1.84) ml/s in the PEC group at 1 month after surgery (P < 0.01), but neither showed any statistically significant difference in the HLC group. Mild to moderate anxiety was prevalent in the patients preoperatively, particularly in those without job or regular income and those with a long disease course or frequent onset, the severity of which was not correlated with age, education or marital status. The HAMA score was decreased from18.65 ± 4.33 before to 12.35 ± 3.63 after surgery in the PEC group (P < 0.01), and from 16.88 ± 2.11 to 6.87 ± 4.36 in the HLC group (P < 0.01). The mean hospital stay was significantly longer in the former than in the latter group ([5.2 + 1.3] vs [3.4 ± 0.5] d, P < 0.01), while the total cost markedly lower ([6.35 ± 1.20] vs [12.72 ± 2.15] thousand RMB ¥, P < 0.05).@*CONCLUSIONS@#Both PEC and HLC are safe and effective for the treatment of post-ejaculation hematuria, with no significant difference in the recurrence rate at 3 and 6 months after operation, but their long-term effect needs further follow-up studies. PEC may increase the risk of negative outcomes of the postoperative urinary flow rate, while HLC has the advantages of better relieving the patient's anxiety, sooner discharge from hospital and earlier recovery from postoperative hematuria, though with a higher total cost than the former.


Subject(s)
Adult , Cautery , Ejaculation , Hematuria/surgery , Holmium , Humans , Laser Therapy , Lasers, Solid-State/therapeutic use , Male , Middle Aged , Treatment Outcome , Young Adult
6.
Article in Chinese | WPRIM | ID: wpr-773168

ABSTRACT

To compare the polysaccharides( LBLP),total flavonoids( LBLTF) and total phenols( LBLTP) of Lycium barbarum leaves from 14 production areas from Ningxia,Qinghai and Gansu at different harvesting time. Our results showed that there were significant difference of LBLP,LBLTF and LBLTP among different production areas. The LBLP collected from Xiangride township production area,Dulan,Qinghai was as high as 15. 02%. The LBLTF collected from Dagele township production area,Geermu,Qinghai was as high as28. 63%. The LBLTP collected from Keluke township production area,Delingha,Qinghai was as high as 16. 7%. There were also significant difference of these 3 components between different harvest periods. The average LBLP in May( 10. 20%) was significantly higher than that in June( 8. 49%). However,the average LBLTF( 17. 71%) and LBLTP( 12. 77%) in June was significantly higher than that in May( 14. 15%,11. 19%). The LBLTF and LBLTP have a positive correlation with the altitude of production areas,but there was no significant association between LBLP and altitude. The LBLP,LBLTF and LBLTP in different production areas and harvesting time was significant difference,the results provide some references for furthermore development and utilization of L. barbarum leaves.


Subject(s)
Analysis of Variance , China , Flavonoids , Geography , Lycium , Chemistry , Phenols , Plant Leaves , Chemistry , Polysaccharides , Seasons
7.
Article in Chinese | WPRIM | ID: wpr-800534

ABSTRACT

Objective@#To study the age of onset of malignant tumors in cancer registration areas in Zhejiang province from 2000 to 2015.@*Methods@#Based on the incidence data of cancer from 14 national cancer registration areas in Zhejiang province between 2000 and 2015, the incidence rate, average/standardized average age of onset, and age-specific incidence proportion were calculated, and standardized by Segi′s world population. The population was divided into 7 age groups (0-29, 30-39, 40-49, 50-59, 60-69, 70-79, ≥80 years old). The average age of onset and age-specific incidence proportion were analyzed by using a linear regression. The change trend of the proportion; the average annual percent change (AAPC) of the incidence rate was calculated by using the Joinpoint log-linear regression model. The birth cohort was constructed by using the standardized incidence rate and the year of onset of cancer, and the distribution characteristics of the age groups were described.@*Results@#From calendar year 2000 to 2015, the average age of onset of malignant tumors in Zhejiang Province decreased from 59.7 to 57.6, with an average annual percent decrease of 0.09 year (P=0.033). After the merge of age groups, the standardized incidence proportion of 30-49 and ≥60 years old group standardization increased by an average annual change of 0.38% and decreased by an average annual change of 0.39%, respectively (both P values <0.001). The results of the log-linear regression model showed that the standardized incidence rate of the 30-39 and 40-49 years old age groups increased rapidly, with the AAPC (95%CI) about 5.2% (4.4%-6.0%) and 3.4% (2.5%-4.3%), respectively. The incidence rate of female increased rapidly, especially for 30-39 and 40-49 years old age groups with the AAPC about 6.2% (5.1%-7.2%) and 4.9% (4.0%-5.9%), respectively. The results of the birth cohort analysis showed that the population with different birth years in the same age groups saw an increasing trend with the increase of the birth year.@*Conclusion@#The age of onset of malignant tumors in the cancer registration areas in Zhejiang Province is getting younger. The incidence rate and number of people with malignant tumors in 30-49 years old age group is evident. These characteristics are more obvious in female than that in male.

8.
Chinese Pharmaceutical Journal ; (24): 1520-1526, 2019.
Article in Chinese | WPRIM | ID: wpr-857914

ABSTRACT

OBJECTIVE: Medication errors (ME) more frequently affect pediatric patients than adults. Chemotherapeutic drug MEs seems more serious and less detected, among which, the most commonly involved chemotherapeutic agent was methotrexate (MTX). To engage multidisciplinary teams of childhood malignancy in a multisite study using proactive risk assessment methods to identify how MTX errors occur and propose risk reduction strategies. METHODS: We recruited doctors, nurses, pharmacists and parents from three children's hospitals in the northeast and southeast China to participate in failure mode and effects analyses (FMEA). An FMEA is a systematic team-based proactive risk assessment approach in understanding ways a process can fail and develop prevention strategies. Steps included diagram the process, collect failure modes /risks and prioritize failure modes, and propose risk reduction strategies. We focused on MTX-use process in and out of hospitals. RESULTS: A multidisciplinary medication safety team was formed of total 66 members. They developed a four-stage flow diagram with four main phases, based on which, 56 potential risks were recognized and 17 were classified as higher risks by the hazard-scoring matrix. The highest priority failure modes in hospital included wrong solvents, wrong frequency label and lake of monitoring; furthermore, errors involving excessive intake of oral MTX after discharge were worth extra attention. Meanwhile, remediation strategies were developed, consisting of constrained and recommended strategies. CONCLUSION: FMEA is a useful tool to identify the risk of MTX MEs and several years later, with the concerted efforts of all the healthcare staff and technicians, we wish to see a reduction in the potential for errors being made and an improvement of children safety.

9.
Chinese Pharmacological Bulletin ; (12): 620-623, 2019.
Article in Chinese | WPRIM | ID: wpr-857251

ABSTRACT

Aim To investigate the effect of rhyncho-phylline on methamphetamine ( METH )-induced condition place preference (CPP) zebrafish and the relationship with GluRl. Methods METH was injected intraperitoneally to zebrafish, and the expression of GluRl in zebrafish brain was detected by immunohisto-chemistry and Western blot. Results METH-induced CPP model was established after the administration of METH (40 mg • kg-1) in zebrafish. Compared to control group, the resident time in white box of model group zebrafish significantly increased; compared to model group, the resident time in white box of ket-amine (150 mg • kg-1) group and rhynchophylline (100 mg • kg-1) group dramatically decreased. Compared to control group, the number of GluRl positive cells and the GluRl expression of model group ze-brafish markedly increased; compared to model group, the number of GluRl positive cells and the GluRl expression of rhynchophylline group zebrafish evidently decreased. Conclusions GluRl is important for METH-induced CPP formation in zebrafish. Further, rhynchophylline reduces GluRl expression in METH-treated zebrafish brain, and its mechanism may be related to GluRl expression in the brain of zebrafish.

10.
Article in Chinese | WPRIM | ID: wpr-817956

ABSTRACT

OBJECTIVE: To investigate the imaging characteristics and their relationship with histopathological changes of pilomyxoid astrocytomas(PMA)in children. METHODS: The CT and MRI findings as well as clinical data of 20 pediatric patients with PMA admitted to Beijing Tiantan Hospital,Capital Medical University between October 2010 and June 2018 were analyzed retrospectively,and their clinical features,pathological and immunohistochemical results were also analyzed.RESULTS: There were 15 males and 5 females in total 20 patients with PMA. Tumors were located at cerebellar hemisphere in 10,cerebral hemisphere in 5,chiasmatic-hypothalamic region in 4 and at the third ventricle in 1. Of 20 patients with PMA,10 presented hypodense on CT images,11 showed mixed signal intensity on both T1 and T2 weighted images on MRI,7 demonstrated hypointensity on T1 WI and 7 had hyperintensity on T2 WI. Absence of diffusion restriction were noticed in 12 tumors. The tumors showed significant inhomogeneous enhancement in 17 cases under MRI enhanced screening. The cases of neoplasm clear margin,necrosis inside tumors and peritumor edema were 18,16 and 11,respectively. Histopathologically,PMA was characterized by the presence of bipolar spindle cells,angiocentric arrangement,prominent myxoid background and the lack of Rosenthal fibers. CONCLUSION: The pediatric PMA has low-grade tumor features and characteristic imaging features. There are several imaging features which are relatively specific for PMAon CT and MRimages,such as heterogeneous texture,clear border,cystic changes of edge and inhomogeneous enhancement. CT and MRI may provide valuable information for preoperative diagnosis,clinical treatment and prognostic evaluation in patients with PMA.

11.
Article in Chinese | WPRIM | ID: wpr-707724

ABSTRACT

Objective To explore the predictive accuracy of two estimated fetal weight ( EFW ) standards ( INTERGROWT H and Hadlock) and Doppler parameters for late-onset fetal growth restriction ( FGR) . Methods A prospective cohort of women with singleton pregnancies who attended research scans and had a livebirth at the obstetrics and gynecology hospital of Fudan University during 32 - 41 weeks of gestation was involved . The markers of ultrasound examinations (including growth measurements ,umbilical artery and middle cerebral artery parameters) were obtained every two weeks . The INTERGROWT H -EFWc and Hadlock-EFWc data were obtained from the last ultrasonography ( within 7 days before delivery) and were used to predict later-onset FGR in a single model or in combined models with other Doppler parameters by logistic regression analyses ,respectively . According to delivery gestation of age and Chinese birth weight (BW) standards ,all cases were divided into a control group ( non-FGR ,BW ≥ 10th % ) and a FGR group ( Late-onset FGR ,BW < 10th % ) . ROC curve analyses were performed to compare the predictive accuracy for the late-onset FGR between the Hadlock-EFWc and INTERGROWT H-EFWc . Results A total of 820 eligible women were identified and 676 had finished the follow-up and were enrolled in this prospective cohort study . Among them ,116 neonates were assigned to the late-onset FGR group ,and 560 as control group ( non-FGR) . The cut-off value of the INTERGROWT H-EFWc was percentile 27 .5 ,at which had a sensitivity and specificity of 71 .4% and 83 .7% . The corresponding sensitivity and specificity were 87 .3% and 82 .8% at a cut-off value of percentile 22 .6 of the Hadlock-EFWc . The Hadlock-EFWc had a higher predictive accuracy for the late-onset FGR than the INTERGROWT H-EFWc ,their AUC were 0 .930 ( 0 .908 - 0 .953 ) and 0 .847 ( 0 .807 - 0 .888 ) ,respectively . The accuracy of Doppler single-parameter ( umbilical artery and middle cerebral artery) for late-onset FGR were low ( AUC < 0 .7) ,but the accuracy of combined model- Ⅰ and Ⅱ were high ( AUC 0 .865 and 0 .936 ,respectively) ,similar to their corresponding EFWc models ,respectively . Conclusions The INTERGROWT H-EFWc could predict effectively for late-onset FGR ,however ,its predictive accuracy is lower than that of the Hadlock-EFWc . The predictive accuracy of Doppler parameters for late-onset FGR are poor ,routine monitoring of non-selected populations is not recommended .

12.
Chinese Pharmacological Bulletin ; (12): 1226-1230, 2018.
Article in Chinese | WPRIM | ID: wpr-705180

ABSTRACT

Aim To explore the effect of rhynchophyl-line on the expression of tyrosine hydroxylase ( TH) in hippocampus of methamphetamine-induced condition place preference ( CPP) mice. Methods Metham- phetamine was injected intraperitoneally to mice, and the expression of TH was observed by immunohisto-chemistry and Western blot. Results The CPP mouse model was established successfully by methamphet-amine ( 4 mg·kg-1) . Ketamine ( 15 mg·kg-1) , rhynchophylline low dosage group (40 mg·kg-1) and rhynchophylline high dosage group ( 80 mg·kg-1) could remove the effect of methamphetamine on CPP mice. The result of immunohistochemistry showed that methamphetamine ( 4 mg·kg-1) could increase the number of TH positive cells in hippocampus while ket-amine (4 mg·kg-1), rhynchophylline (40, 80 mg· kg-1) group could attenuate the change. Western blot-ting indicated the expression of TH of model group in-creased significantly, whereas ketamine ( 15 mg· kg-1) , rhynchophylline ( 40, 80 mg·kg-1) group presented less expression. Conclusions The CPP in-duced by methamphetamine in mice may be inhibited to some extent by rhynchophylline, and its mechanism may be associated with the expression of TH.

13.
Article in Chinese | WPRIM | ID: wpr-851893

ABSTRACT

Atrial fibrillation (AF) is the most common type of arrhythmia in clinic. At present, the therapies of AF by western medicine are fewer with obvious side effects. It is expected to improve the curative effect and reduce side effects by the combination of traditional Chinese medicine (TCM) and western medicine. The research progresses of Chinese materia medica (CMM) treatment for AF were summarized, and the biological molecular network was carried out for the analysis of blood components of ginsenoside from the key CMM Panax ginseng in AF treatment. The blood components of ginsenoside in network analysis including targets searching, network construction, and GO biological processes analysis were selected to explore the possible mechanism in treating AF. It indicated that the possible mechanism might be related to apoptosis regulation, cell membrane potassium ion flow, and so on.

14.
Article in Chinese | WPRIM | ID: wpr-697009

ABSTRACT

Objective To investigate the quality of the chronic kidney information found on websites. Methods Three search engines were searched for relevant chronic kidney websites. The independent raters use HON code(The Health On Net Code of Conduct for medical and health Web sites, HON code) and DISCERN instrument to evaluate the chronic kidney websites in Chinese. Results A total of 18 websites were included into our study.The overall mean quality of HON code was 3.6±1.6.The overall mean quality of DISCERN instrument was 32.3 ± 10.8. In the research site, the number of sites built by nonprofits accounted for only 5.6%(1/18). Conclusions The information quality on chronic kidney found online was of poor.The main reason is health websites contain lots of advertisement,and it is rare that non-profit websites can provide medical information which is suitable for general public,the overall reliability is poor,Chinese health websites need to put efforts on developing the quality of health information.

15.
Article in Chinese | WPRIM | ID: wpr-691132

ABSTRACT

<p><b>OBJECTIVE</b>Establishing a rabbit model of vertebral artery type of cervical spondylosis(CSA) and to observe the characteristics of timeliness in improving the blood flow of vertebral artery by massage, and discusse the material basis of this timeliness based on NPY and ET-1.</p><p><b>METHODS</b>Fifty New Zealand healthy and white rabbits, 6-month-old, the body mass of (2.0±0.5) kg, with half males and half females, were randomly divided into blank group, model group, three massage groups(including massage for 10 min, 20 min, and 30 min group by random number table), 10 rabbits in each group. In addition to the blank group, CSA rabbit model was made by injection of sclerosing agent in other groups. The rabbits of massage for 10 min, 20 min, and 30 min groups received the massage therapy of corresponding duration, one times a day, continuous 10 days. The blood flow of vertebral artery in each group was detected by PeriFlux5000 laser doppler, and the contents of NPY and ET-1 in serum were detected by ELISA before and after treatment.</p><p><b>RESULTS</b>Changes in blood flow of vertebral artery before and after treatment:there was no significant difference between model group and massage for 10 min group;there was significant difference between model group and massage for 20, 30 min groups(<0.01); there was significant difference between massage for 10 min group and massage for 20, 30 min groups(<0.01); there was no significant difference between massage for 20 min group and massage for 30 min group. Changes of NPY content before and after treatment: there was significant difference between model group and massage for 10 min group(<0.05); there was significant difference between model group and massage for 20, 30 min groups(<0.01); there was significant difference between massage for 10 min group and massage for 20, 30 min groups(<0.01); there was no significant difference between massage for 20 min group and massage for 30 min group. Changes of ET-1 content before and after treatment:there was no significant difference between model group and massage for 10 min group;there was significant difference between model group and massage for 20, 30 min groups(<0.01); there was significant difference between massage for 10 min group and massage for 20 min, 30 groups (<0.01); there was no significant difference between massage for 20 min group and massage for 30 min group.</p><p><b>CONCLUSIONS</b>Massage needed 20 min for rabbits with CSA can only significantly improve the blood flow of vertebral artery. However, prolonging the time of massage has no obvious effect. The material basis of this timeliness characteristic of massage is closely related to the change of NPY and ET-1 levels in serum.</p>

16.
Article in Chinese | WPRIM | ID: wpr-660260

ABSTRACT

Objective To observe the inhibitory effect of ginsenoside RG3 on choroidal neovascularization in vitro and in vivo.Methods MTT assay was used to evaluate the inhibitory effect of ginsenoside RG3 on human umbilical vein endothelial cells (HUVEC) proliferation in vitro,and HUVEC were divided into normal group,in which the cells were cultured in DMEM/F-12 medium containing 10% fetal bovine serum,control group with its medium containing 2 g · L-1 dimethyl sulfoxide (DMSO) and different concentrations of ginsenoside RG3 administration group (12.5 μmol · L-1,25.0 imol · L-1,50.0 μmol · L-1,100.0 μmol · L-1).Then the absorbance value was measured after 6 h.Then,a small amount of HUVEC was collected again and divided into control group with its medium containing 2 g · L-1 DMSO and 100.0 μrnol · L-1 ginsenoside RG3 group for detecting the inhibitory effect of ginsenoside RG3 on tubular formation and vascular endothelial growth factor (VEGF) protein expression by Western blots.In vivo,20 male C57BL/6J mice were collected and randomly divided into control group and ginsenoside RG3 group.After 2 weeks,followed by establishment of model with a semiconductor laser,fundus fluorescein angiography was performed on the 1 st day and 21 st days after treatment.Results MTT results showed that absorbance value of the normal group,control group,12.5 μnol · L-1,25.0 μmol · L-1,50.0 μmol · L-1,100.0 μmol · L-1 ginsenoside RG3 group was 0.43 +0.17,0.43 ±0.05,0.33 +0.02,0.24 +0.02,0.18 ±0.01,0.15 ±0.01 accordingly,and there was no significant difference between the control group and the normal group (all P > 0.05),but the difference between the other group and control group was statistically significant (all P < 0.05).Tubular formation assay showed that the number of tubular formation in the control group and 100.0 μmol · L-1 ginsenoside RG3 group was 72.5 + 5.56 and 11.33 ± 3.71,respectively,and the difference was statistically significant (P < 0.01).Western blots showed that the relative expression of VEGF in 100.0 μmol · L-1 ginsenoside RG3 group (0.14 ±0.01) was significantly lower than that in the control group (0.46 ±0.01),and the difference was statistically significant (P <0.01).In vivo fundus fluorescein anglography showed that the fluorescein leakage area of ginsenoside RG3 group was lower than that of the control group.Conclusion Ginsenosid RG3 can inhibit the formation of choroidal neovascularization by inhibiting the expression of VEGF in vitro and in vivo.

17.
Asian Journal of Andrology ; (6): 473-476, 2017.
Article in Chinese | WPRIM | ID: wpr-842736

ABSTRACT

The laparoendoscopic single-site (LESS) technique is the latest technical innovation in laparoscopic surgery to undergo exponential development in urology. This study undertaken to illustrate our initial experience LESS radical prostatectomy (RP) and analyze early outcomes. Nineteen patients diagnosed with prostate cancer underwent LESS-RP in our institute. The patients were divided into two groups: conventional LESS and transurethral assistant LESS. Preoperative, perioperative, postoperative, pathologic, and functional outcomes data were assessed. With the help of a transurethral assistant, the mean operation and anastomosis time were decreased markedly. No focal positive margins were encountered. No prostate-specific antigen recurrence was detected 1 month postoperatively. Complete continence recovery (no pad) was observed in 32% of the patients at 1 month after the operation. No intraoperative and postoperative complications were reported. LESS-RP is a feasible and effective surgical procedure for treatment of prostate cancer. Moreover, transurethral assistant LESS could reduce the difficulty of LESS-RP and shorten the operation time.

18.
Article in Chinese | WPRIM | ID: wpr-618390

ABSTRACT

Objective To explore the application value of INTERGROWTH-21st standard evaluate intrauterine growth restriction (IUGR).We aimed at predicting IUGR with ultrasonic fetal growth measurements.Methods A prospective cohort of singleton pregnancy scanned after 28 gestational weeks was constructed.The Z-score values of growth measurements were calculated using the INTERGROWTH-21st standard.Logistic regression equation was used to establish the prediction formula.Finally,the new formula was compared with the traditional Hadlock method to predict the accuracy of IUGR.We assessed outcomes for consenting participants who attended research scans and delivered at the Obstetrics and Gynecology Hospital of Fudan University.Results A total of 834 patients fulfilling the inclusion criteria were included.After the exclusion of 143 lost cases,565 of control cases and 126 of IUGR cases were obtained.The sensitivity,specificity,positive predictivevalue,negative predictive value,false positive rate and false negative rate of prediction model wererespectively:88.9 %,85.4 %,57.7 %,97.2 %,14.6 %,11.1 %.In contrast,the values of Hadlock wererespectively:81.7%,82.7%,51.2%,95.3%,17.3%,18.3%.Conclusions This study shows aprediction model of IUGR.INTERGROWTH-21st standard improved the diagnostic accuracy of IUGR,especially enhanced the positive predictive value.

19.
Article in Chinese | WPRIM | ID: wpr-618368

ABSTRACT

Objective:To investigate cancer mortality in Zhejiang cancer registration areas from 2010 to 2014. Methods:Cancer regis-tration data were collected from 14 cancer registries of Zhejiang province. The number of cases, crude rates, proportions, age stan-dardized rates, cumulative rates, cut rates, age-specific rates, top 10 mortality ranks, and annual percentage change (APC) of cancer mortality were calculated. Results:A total of 108,514 cancer deaths in Zhejiang cancer registration areas from 2010 to 2014, including 70,578 males and 37,936 females, were recorded. The crude mortality rate was 186.06/105. The standardized mortality rates in Chi-nese (ASMRC) and world populations were 103.02/105 and 101.73/105, respectively. The cumulative mortality rate (0-74 years old) was 11.46%. The ratio of ASMRC in male and female was 1.95, and that in urban and rural areas was 0.93. Age-specific cancer mortality in-creased significantly after 40 years old and peaked at the 85+age group. The crude cancer mortality increased from 2010 (182.85/105) to 2014 (191.00/105) by 4.46%. After age standardization, the mortality rate standardized by Chinese population was reduced from 107.85/105 to 100.60/105. The APC of ASMRC was-1.96%(P=0.017). The top three mortality cancers were lung cancer, liver cancer and gastric cancer. The top 10 cancers accounted for 89.51%of all cancer deaths. The mortality rates in different age groups were inconsis-tent. Leukemia was the main cancer type among 0~14-year-old population. Liver cancer was the most common cancer type in 15~44-year-old population. Lung cancer was the main cancer in population above 45 years old. The ASMRCs of liver cancer, gastric cancer and esophageal cancer decreased, whereas those of pancreatic cancer, prostate cancer and ovarian cancer increased. Conclusion:Can-cer mortality in Zhejiang cancer registration areas increased, which revealed that the number of cancer deaths will continue to in-crease. However, the ASMRC decreased, which indicated that cancer prevention in Zhejiang province had achieved certain effects. Fur-ther measurements, such as health education, cancer screening, and early detection and treatment, should be improved to reduce the danger of cancer.

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Journal of Experimental Hematology ; (6): 1487-1492, 2017.
Article in Chinese | WPRIM | ID: wpr-301701

ABSTRACT

<p><b>OBJECTIVE</b>To establish a myelodysplastic syndrome transformed to leukemia cell line stably expressing green fluorescent protein (GFP), and to evaluate its biological characteristics and applications.</p><p><b>METHODS</b>SKM-1 cells were transfected by lentiviral particles with vector of GFP. The GFP positive single cell clone was isolated by limiting dilution and continued being cultured. The cells were injected into mice subcutaneously and were screened in vivo. Then SKM-1/GFP cells were obtained after tumour plaque was separated and cultivated. The cell morphology was observed by fluorescence microscopy. The GFP expression was further detected by flow cytometry. The cell proliferation was analysed by CCK-8 assay. SKM-1/GFP cells were inoculated to subcutaneous tissue of the immunodeficiency mice. The growth and invasion of the tumour were observed after tumour formation.</p><p><b>RESULTS</b>No differences in cell morphology and growth characteristics were observed between SKM-1 cells and SKM-1/GFP cells. The rate of GFP expression was 100%. No differences in cell proliferation were observed between SKM-1 cells and SKM-1/GFP cells. The tumour mass was observed after 14 days of subcutaneous vaccination in NOD/SCID mice. Spontaneous fluorescence from plaque was observed by living fluorescence microscopy at 30th day after vaccination. Homogenous GFP positive cells were observed by fluorescence microscopy in the frozen section of tumour mass. The invasion of SKM-1/GFP cells was also detected in heart, liver, stomach and kidney of mice.</p><p><b>CONCLUSION</b>A myelody-splastic syndrome transformed to leukemia cell line stably expressing green fluorescent protein has been established successfully, which can track tumor cell sensitively and can be applied to the research of minimal residual leukemia. The establishment of SKM-1/GFP cells may serve as a powerful means for studing myelodysplastic syndrome transformation.</p>

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