Your browser doesn't support javascript.
Show: 20 | 50 | 100
Results 1 - 1 de 1
Chinese Journal of Pathology ; (12): 513-517, 2004.
Article in Chinese | WPRIM | ID: wpr-283483


<p><b>OBJECTIVE</b>To develop a protocol for gene rearrangement study in non-Hodgkin's lymphoma (NHL) by PCR-directed gel-scan method and to set up quantitative criteria for IgH gene rearrangement which can be applied in the follow up of lymphoma patients.</p><p><b>METHODS</b>IgH gene rearrangement studies were carried out in 96 cases of B-cell NHL. The detection rate of clonality was evaluated. Sixty-five cases of IgH gene rearranged cases proven by FR3A-directed PCR and PAGE and 8 cases of benign lymphoid tissues (5 cases of reactive lymphoid hyperplasia, 3 cases of chronic tonsillitis), 5 cases of normal peripheral blood mononuclear cells were analyzed by gel-scan method and the proportion of h1/h2 (heights of peak1 and peak2 of gel-scan) was calculated.</p><p><b>RESULTS</b>The detection rate of IgH gene clonality was up to 68% using primer FR3A in the 96 B-cell NHL cases. The detection rate was up to 61% using primer FR2A. With a combination of primers FR3A and FR2A, the detection rate increased to 83%. Gel-scan curve showed that the value of h1/h2 was greater than 3 in all the 65 cases with IgH gene rearranged. In the 8 benign lymphoid tissue cases showed h1/h2 < 1.5, 5 cases with normal peripheral blood mononuclear cells showed a bell-shaped curve.</p><p><b>CONCLUSIONS</b>In the gel-scan curve of gene rearrangement studies in non-Hodgkin's lymphoma samples, the value of h1/h2 greater than 3 represents a true clonal proliferation. The peaks with relative heights less than 1.5 may not be significant and likely represent polyclonal cell population. A value between 1.5 and 3 however requires clinical follow-up. The success rate of rearrangement studies in B-cell NHL can be increased by using a combination of primers FR3A and FR2A.</p>

Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Humans , Male , Middle Aged , DNA Primers , Electrophoresis, Gel, Two-Dimensional , Gene Rearrangement, B-Lymphocyte, Heavy Chain , Genes, Immunoglobulin , Immunoglobulin Heavy Chains , Genetics , Lymphoma, B-Cell , Genetics , Allergy and Immunology , Pathology , Lymphoma, B-Cell, Marginal Zone , Genetics , Allergy and Immunology , Pathology , Lymphoma, Large B-Cell, Diffuse , Genetics , Allergy and Immunology , Pathology , Polymerase Chain Reaction