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1.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 26-36, 2024.
Article in Chinese | WPRIM | ID: wpr-1003405

ABSTRACT

ObjectiveTo investigate the effect of Yishen Tongluo prescription (YSTLP) on apoptosis of renal tubular epithelial cells and explore the mechanism based on endoplasmic reticulum stress pathway of protein kinase R-like endoplasmic reticulum kinase (PERK)/activating transcription factor 4 (ATF4)/transcription factor C/EBP homologous protein (CHOP). MethodThe db/db mice were randomly divided into model group, valsartan group (10 mg·kg-1), and low, middle, high-dose YSTLP groups (1, 2.5, 5 g·kg-1). Samples were collected after eight weeks of drug intervention. In addition, db/m mice in the same litter served as the control group. Human renal tubular epithelial cells (HK-2) were cultured in vitro and divided into the control group, advanced glycated end-product (AGE) group, and AGE + low, middle, and high-dose YSTLP groups (100, 200, 400 mg·L-1). TdT-mediated dUTP nick end labeling (TUNEL) staining was used to detect the apoptosis rate of HK-2 cells. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was conducted to detect the viability of HK-2 cells. Calcium fluorescence probe staining and luciferase reporter gene method were adopted to detect the luciferase activity of folded protein response element (UPRE) and endoplasmic reticulum stress. Immunohistochemical (IHC) analysis was carried out to measure the protein expressions of phosphorylated PKR (p-PERK), CHOP, and ATF4. Real-time polymerase chain reaction (Real-time PCR) was used to measure the mRNA expression levels of CHOP and X-box binding protein 1 (XBP1) in mouse kidney and HK-2 cells. Western blot was used to detect the protein expression level of p-PERK, PERK, CHOP, ATF4, B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), and cleaved Caspase-3 in mouse kidney and HK-2 cells. ResultIn the cellular assay, HK-2 cell viability was significantly reduced, and the apoptosis rate was elevated in the AGE group compared with the control group (P<0.01). The mRNA and protein expression levels of apoptosis-related factor Bcl-2 were significantly reduced (P<0.01), and those of Bax were significantly increased (P<0.01). The protein expression level of cleaved Caspase-3 was significantly increased (P<0.01). Compared with the AGE group, YSTLP administration treatment resulted in elevated cell viability and reduced apoptosis rate (P<0.01). The mRNA and protein expression levels of Bcl-2 were significantly elevated in a time- and dose-dependent manner (P<0.01), and those of Bax were significantly reduced in a time- and dose-dependent manner. The protein expression level of cleaved Caspase-3 was significantly reduced in a time- and dose-dependent manner (P<0.01). The intracellular Ca2+ imbalance and UPRE luciferase fluorescence intensity were increased in the AGE group compared with the control group (P<0.01). The mRNA levels of endoplasmic reticulum stress-related factors CHOP and XBP1 were significantly increased (P<0.01), and the protein expression levels of p-PERK, CHOP, and ATF4 were significantly increased (P<0.05). Compared with the AGE group, YSTLP effectively improved intracellular Ca2+ imbalance in HK-2 cells and decreased UPRE luciferase fluorescence intensity in a dose-dependent manner (P<0.01). It reduced the mRNA levels of endoplasmic reticulum stress-related factors CHOP and XBP1 (P<0.01) and the protein expression levels of intracellular p-PERK, CHOP, and ATF4 in a dose- and time-dependent manner (P<0.01). In animal experiments, the protein expression level of Bcl-2 was significantly reduced(P<0.01), and that of cleaved Caspase-3 and Bax was significantly increased in the model group compared with the control group (P<0.05). The protein expression level of Bcl-2 was dose-dependently elevated, and that of cleaved Caspase-3 and Bax was dose-dependently decreased in the YSTLP groups compared with the model group (P<0.01). Compared with the control group, the mRNA expression levels of CHOP and XBP1 were significantly elevated in the model group (P<0.05, P<0.01), and the protein expression levels of p-PERK, CHOP, and ATF4 were significantly increased (P<0.05). Compared with the model group, YSTLP significantly decreased the mRNA expression levels of CHOP and XBP1 (P<0.01) and the protein expression levels of p-PERK, CHOP, and ATF4 (P<0.01). ConclusionYSTLP can effectively inhibit endoplasmic reticulum stress and improve apoptosis of renal tubular epithelial cells, and its mechanism may be related to the regulation of the PERK/AFT4/CHOP pathway.

2.
Rev. bras. med. esporte ; 29: e2023_0016, 2023. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1431660

ABSTRACT

ABSTRACT Introduction: Dedicated fitness training has greatly influenced many qualities required of volleyball players, but specific upper limb training is still poorly documented. Objective: Study the impacts of fitness training on upper limb strength and functional impacts during volleyball players' ball throws. Methods: Twenty volleyball students were randomly divided into experimental and control groups for a six-week experiment. The experimental group mainly performed a training protocol involving ball throws while the control group continued with traditional training methods. The data regarding functional kinematics and fitness performance were collected and statistically compared. Results: In the experimental group, shoulder flexor strength evidenced an increase from 0.41±0.19 J/kg to 1.29±0.19 J/kg; elbow flexor strength elevated from 0.38±0.23 J/kg to 1.61±0.02 J/kg. On the specific functional performance, the peak score increased from 4.84±1.44 to 7.47±1.43; The overall score increased from 9.05±1.70 to 13.31±1.72. It was noted that the rate of increase of each index was more significant than that of the control group. Conclusion: Dedicated fitness training can improve the upper limb strength and hitting effect of volleyball players, and its combination with special strength training is recommended to improve the overall skills of athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.


RESUMO Introdução: O treinamento dedicado de aptidão física tem mostrado grande influência sobre muitas qualidades requisitadas aos jogadores de voleibol, porém o treinamento específico em membros superiores ainda é pouco documentado. Objetivo: Estudar os impactos do treinamento de aptidão física sobre a força dos membros superiores e os impactos funcionais durante os arremessos de bola dos jogadores de voleibol. Métodos: Vinte estudantes de voleibol foram divididos aleatoriamente em grupo experimental e controle para uma experiência de 6 semanas. O grupo experimental realizou principalmente um protocolo de treinamento envolvendo arremessos de bola enquanto o grupo de controle prosseguiu com os métodos tradicionais de treinamento. Dados referentes a cinemática funcional e desempenho de aptidão física foram coletados e comparados estatisticamente. Resultados: No grupo experimental, a força do flexor do ombro evidenciou um aumento de 0,41±0,19 J/kg para 1,29±0,19 J/kg; a força do flexor do cotovelo elevou-se de 0,38±0,23 J/kg para 1,61±0,02 J/kg. Sobre o desempenho funcional específico, a pontuação do pico aumentou de 4,84±1,44 para 7,47±1,43; A pontuação geral aumentou de 9,05±1,70 para 13,31±1,72. Notou-se que a taxa de aumento de cada índice foi mais significativa do que a do grupo de controle. Conclusão: O treinamento dedicado de aptidão física pode melhorar a força dos membros superiores e o efeito de acertos dos jogadores de vôlei, sendo recomendada a sua combinação com o treinamento especial de força para melhorar as habilidades gerais dos atletas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción: El entrenamiento físico específico ha demostrado una gran influencia en muchas cualidades exigidas a los jugadores de voleibol, pero el entrenamiento específico de extremidades superiores está aún poco documentado. Objetivo: Estudiar las repercusiones del entrenamiento físico en la fuerza de los miembros superiores y los impactos funcionales durante los lanzamientos de balón de los jugadores de voleibol. Métodos: Veinte estudiantes de voleibol fueron divididos aleatoriamente en grupo experimental y de control para un experimento de 6 semanas. El grupo experimental realizó principalmente un protocolo de entrenamiento que incluía lanzamientos de balón, mientras que el grupo de control continuó con los métodos de entrenamiento tradicionales. Se recogieron y compararon estadísticamente los datos relativos a la cinemática funcional y el rendimiento físico. Resultados: En el grupo experimental, la fuerza flexora del hombro evidenció un aumento de 0,41±0,19 J/kg a 1,29±0,19 J/kg; la fuerza flexora del codo se elevó de 0,38±0,23 J/kg a 1,61±0,02 J/kg. Respecto al rendimiento funcional específico, la puntuación máxima aumentó de 4,84±1,44 a 7,47±1,43; la puntuación global aumentó de 9,05±1,70 a 13,31±1,72. Se observó que el índice de aumento de cada índice fue más significativo que el del grupo de control. Conclusión: El entrenamiento físico dedicado puede mejorar la fuerza de las extremidades superiores y el efecto de golpeo de los jugadores de voleibol, y se recomienda su combinación con un entrenamiento especial de fuerza para mejorar las habilidades generales de los atletas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

3.
Rev. bras. med. esporte ; 29: e2022_0699, 2023. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1423390

ABSTRACT

ABSTRACT Introduction: Volleyball requires athletes to have high global sport capacity. Compound exercises require more than one muscle group to complete a given movement. They are ideal for jump development in volleyball because they replicate how the athlete's body moves naturally. However, the attributes relevant to this technique are not fully understood. Objective: Study compound exercise's effects on volleyball players' jumping ability. Methods: The controlled experiment method was used in 30 volunteers equally distributed in two groups, with a duration of 8 weeks. The control group received traditional training. The experimental group received training sessions directed by compound exercises. The sessions were applied twice a week, lasting 80 minutes per session, including 15 minutes of warm-up and 10 minutes of cool-down. The indicators were collected individually before and after the experiment, and the data were analyzed and statistically confronted. Results: The experimental group showed better results on standing vertical jump height, touch height, and blocking height after the compound exercise. Conclusion: The proposed compound exercise can improve players' physical quality, impacting their professional quality, to ensure sporting interest. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.


RESUMO Introdução: O voleibol exige que os atletas tenham alta capacidade esportiva global. Os exercícios compostos requerem mais de um grupo muscular trabalhando em conjunto para completar um determinado movimento, sendo ideal para o desenvolvimento de salto no esporte do voleibol por replicar a maneira como o corpo do atleta move-se naturalmente. Porém, os atributos relevantes para essa técnica não estão totalmente esclarecidos. Objetivo: Estudar os efeitos do exercício composto na capacidade de salto dos jogadores de voleibol. Métodos: Utilizou-se o método de experimento controlado em 30 voluntários igualmente distribuídos em dois grupos, com duração de 8 semanas. O grupo controle recebeu o treinamento tradicional. Treinamentos direcionados foram direcionados por exercícios compostos ao grupo experimental. A sessões foram aplicadas duas vezes por semana, com duração de 80 minutos por sessão, incluindo 15 minutos de aquecimento e 10 minutos resfriamento. Os indicadores forem coletados individualmente antes e depois do experimento, os dados foram analisados e confrontados estatisticamente. Resultados: O grupo experimental apresentou melhores resultados sobre a altura do salto vertical em pé, altura de toque e altura de bloqueio após o exercício composto. Conclusão: O exercício composto proposto pode melhorar a qualidade física dos jogadores, impactando na qualidade profissional, com a premissa de garantir o interesse esportivo. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción: El voleibol exige que los atletas tengan una alta capacidad deportiva global. Los ejercicios compuestos requieren más de un grupo muscular trabajando en conjunto para completar un movimiento determinado, siendo ideal para el desarrollo del salto en el deporte del voleibol por replicar la forma en que el cuerpo del atleta se mueve naturalmente. Sin embargo, los atributos relevantes para esta técnica no se conocen del todo. Objetivo: Estudiar los efectos del ejercicio compuesto sobre la capacidad de salto de los jugadores de voleibol. Métodos: Se utilizó el método de experimento controlado en 30 voluntarios distribuidos equitativamente en dos grupos, con una duración de 8 semanas. El grupo de control recibió formación tradicional. El grupo experimental recibió sesiones de entrenamiento dirigidas por ejercicios compuestos. Las sesiones se aplicaron dos veces por semana, con una duración de 80 minutos por sesión, incluidos 15 minutos de calentamiento y 10 minutos de enfriamiento. Los indicadores se recogieron individualmente antes y después del experimento, se analizaron los datos y se confrontaron estadísticamente. Resultados: El grupo experimental mostró mejores resultados en la altura de salto vertical de pie, la altura de toque y la altura de bloqueo tras el ejercicio compuesto. Conclusión: El ejercicio compuesto propuesto puede mejorar la calidad física de los jugadores, repercutiendo en la calidad profesional, con la premisa de garantizar el interés deportivo. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

4.
Chinese Journal of Primary Medicine and Pharmacy ; (12): 810-813, 2023.
Article in Chinese | WPRIM | ID: wpr-991824

ABSTRACT

Objective:To investigate the relationship between the peak load of Epstein-Barr virus (EPV) and live function damage in children with infectious mononucleosis caused by EPV.Methods:Eighty children with infectious mononucleosis caused by EPV who received treatment in Pingxiang People's Hospital from January 2018 to December 2021 were included in this study. Five mL of venous blood was taken from each child for detecting the peak load of EBV-DNA and liver function indicators. These children were divided into a low-load group ( n = 25, EBV-DNA load < 10 4 copies/mL), a medium-load group ( n = 34, EBV-DNA load of 10 4-10 5 copies/mL), and a high-load group ( n = 21, EBV-DNA load > 10 5 copies/mL) according to the peak EBV-DNA load. The relationships between different peak loads of EBV-DNA and live function, age, and sex were analyzed. Results:The rate of liver dysfunction in the high-load group [85.71% (18/21)] was significantly higher than [38.24% (13/34)] in the medium-load group and [20.00% (5/25)] in the low-load group ( χ2 = 11.90, 19.71, P = 0.001, P < 0.001). Alanine aminotransferase and aspartate aminotransferase levels in the high-load group were (156.24 ± 13.21) U/L and (171.69 ± 13.49) U/L, respectively, which were significantly higher than (125.89 ± 10.54) U/L and (143.26 ± 10.29) U/L in the medium-load group and (89.64 ± 6.75) U/L and (64.89 ± 5.74) U/L] in the low-load group (all P < 0.001). There was no significant difference in the peak load of EBV-DNA between children of different ages and between children of different sexes (both P > 0.05). Conclusion:Children with infectious mononucleosis caused by EPV have a high EBV-DNA peak load. A higher peak load of EVB-DNA indicates a higher risk of liver function damage. More attention should be paid in clinical practice. Effective diagnosis and treatment should be performed in time to control the patient's condition as early as possible.

5.
Journal of Pharmaceutical Analysis ; (6): 421-429, 2023.
Article in Chinese | WPRIM | ID: wpr-991155

ABSTRACT

Chiral metal-organic frameworks(CMOFs)with enantiomeric subunits have been employed in chiral chemistry.In this study,a CMOF formed from 6-methoxyl-(8S,9R)-cinchonan-9-ol-3-carboxylic acid(HQA)and ZnCl2,{(HQA)(ZnCl2)(2.5H2O)}n was constructed as a chiral stationary phase(CSP)via an in situ fabrication approach and used for chiral amino acid and drug analyses for the first time.The{(HQA)(ZnCl2)(2.5H2O)}n nanocrystal and the corresponding chiral stationary phase were systematically characterised using a series of analytical techniques including scanning electron microscopy,X-ray diffraction,Fourier transform infrared spectroscopy,circular dichroism,X-ray photoelectron spectros-copy,thermogravimetric analysis,and Brunauer-Emmett-Teller surface area measurements.In open-tubular capillary electrochromatography(CEC),the novel chiral column exhibited strong and broad enantioselectivity toward a variety of chiral analytes,including 19 racemic dansyl amino acids and several model chiral drugs(both acidic and basic).The chiral CEC conditions were optimised,and the enantioseparation mechanisms are discussed.This study not only introduces a new high-efficiency member of the MOF-type CSP family but also demonstrates the potential of improving the enantiose-lectivities of traditional chiral recognition reagents by fully using the inherent characteristics of porous organic frameworks.

6.
Tissue Engineering and Regenerative Medicine ; (6): 753-766, 2023.
Article in English | WPRIM | ID: wpr-1003134

ABSTRACT

METHODS@#Rat BMSCs were isolated, cultured and identified. The time-dependent expressions of TSP-2 and Sox9 in BMSCs under a dynamic mechanical pressure of 0–120 kPa at 0.1 Hz for 1 h were tested by qPCR and Western blotting. The role of TSP-2 in chondrogenic differentiation of BMSCs under mechanical pressure was validated by using small interfering RNA. The impact of TSP-2 and mechanical pressure on chondrogenesis were detected and the downstream signaling molecules were explored using Western blotting. @*RESULTS@#Mechanical pressure stimulation of 0–120 kPa for 1 h significantly upregulated the expression of TSP-2 in BMSCs. The expression of the chondrogenesis markers Sox9, Aggrecan, and Col-II were all upregulated under dynamic mechanical pressure or TSP-2 stimulation. Additional exogenous TSP-2 may potentiate the chondrogenic effect of mechanical stimulation. After knock down TSP-2, the upregulation of Sox9, Aggrecan and Col-II under mechanical pressure was inhibited. The NF-jB signaling pathway responded to both dynamic pressure and TSP-2 stimulation, and the cartilage-promoting effect was blocked by an NF-jB signaling inhibitor. @*CONCLUSION@#TSP-2 plays an essential role in the chondrogenic differentiation of BMSCs under mechanical pressure. NF-jB signaling is involved in the mechano-chemical coupling of TSP-2 and mechanical pressure for the chondrogenic differentiation of BMSCs.

7.
China Pharmacy ; (12): 2835-2840, 2023.
Article in Chinese | WPRIM | ID: wpr-999214

ABSTRACT

OBJECTIVE To explore the mechanism of Yishen tongluo formula (YSTLF) in improving abnormal lipid metabolism based on the sterol regulatory element binding proteins (SREBPs) pathway. METHODS Using C57BLKS/J (db/db) mice as model and C57BLKS/J (db/m) mice as normal control, the mechanism of 1, 2.5 and 5 g/kg YSTLF improving abnormal lipid metabolism of db/db mice was investigated by determining the liver coefficient, the contents of serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL) and high-density lipoprotein (HDL), observing steatosis and lipid accumulation in liver tissue of mice, detecting the protein expressions of SREBP-1 and SREBP-2 as well as mRNA transcription levels of Srebp- 1c, Srebp-2 and their downstream lipid metabolism-related target genes (Fasn, Acc1, Scd5, Fads1, Hmgcr, Dhcr24, Insig-1, Fdps) in liver tissue of mice. Using low-fat cultured human liver cancer cell HepG2 as an in vitro cell model for abnormal lipid metabolism, and 25-HC (SREBPs inhibitor, 10 μmol/L) as the control, the effects of 125, 250 and 500 μg/mL YSTLF on protein expressions of SREBP-1 and SREBP-2 as well as mRNA transcription of SREBP-1c, SREBP-2 and their downstream lipid metabolism-related target genes were investigated to verify the mechanism in vitro. RESULTS 1, 2.5, 5 g/kg YSTLF significantly reduced the levels of TC, TG and LDL, the percentage of lipid droplet-positive region in liver tissue and liver coefficient, significantly down-regulated protein expressions of Pre-SREBP-1, n-SREBP-1, Pre-SREBP-2 and n-SREBP-2, and mRNA transcription of Srebp-1c, Srebp-2 and their downstream target genes in liver tissue, while significantly increased HDL level, with statistical significance (P<0.05 or P<0.01). In the cell experiment in vitro, the expressions of the above-mentioned proteins and genes in the cells treated with YSTLF at 125, 250 and 500 μg/mL for 24 hours were consistent with those in the animal experiment; there was no significant difference in the expressions of the above-mentioned proteins and genes between inhibitor control group and 250, 500 μg/mL YSTLF groups (P>0.05). CONCLUSIONS YSTLF can regulate the expression of transcription factor SREBPs, so as to inhibit the high expression of fatty acid and cholesterol synthesis-related genes, promote the degradation of TC and TG, improve the abnormality of lipid metabolism and inhibit lipid accumulation, thus playing the role of lipid-lowering.

8.
Chinese Journal of Nuclear Medicine and Molecular Imaging ; (6): 343-348, 2023.
Article in Chinese | WPRIM | ID: wpr-993602

ABSTRACT

Objective:To develop a tetramer probe targeting fibroblast activation protein (FAP), named 1, 4, 7, 10-tetraazacyclododecane-1, 4, 7, 10-tetraacetic acid (DOTA)-4P(FAP inhibitor (FAPI)) 4, evaluate its biodistribution and PET image in FAP-positive-tumor bearing nude mice, and explore its feasibility as a novel radio-regent for treatment of FAP-positive tumor. Methods:FAP tetramer probe was constructed on the FAPI-46 motif with four mini-polyethylene glycol (PEG)(PEG 3) spacers between the four FAPI motifs, denoted as 4P(FAPI) 4. DOTA was used as the chelator for radiolabeling with 68Ga and 177Lu. The FAP binding characteristics were test by in vitro cell competitive binding experiment. Small-animal PET, in vivo biodistribution, and radionuclide targeting therapy were performed in HT-1080-FAP tumor bearing nude mice ( n=39). Independent-sample t test was performed to analyze tumor uptake data, and two-factor repeated measures analysis of variance was utilized to compare tumor volume data in radioactive isotope therapy. Results:Cell experiment showed that FAPI-tetramer and FAPI-monomer had similar half maximal inhibitory concentration values (3.29 and 2.15 nmol/L). 68Ga/ 177Lu radiolabeled FAPI-tetramer had better tumor uptake and retention than FAPI-monomer in small-animal PET and in vivo biodistribution experiment, with the tumor uptake for 177Lu-DOTA-4P(FAPI) 4 and 177Lu-FAPI-46 at 48 h of (18.72±1.32) vs (2.72±1.20) percentage activity of injection dose per gram of tissue (%ID/g) ( t=15.55, P<0.001). 177Lu-DOTA-4P(FAPI) 4 group showed best anti-tumor efficacy compared with 177Lu-FAPI-46 and control group in radionuclide targeting therapy. On the 2nd day after the start of treatment, the tumor volume in the tetramer treatment group was significantly smaller than that in the control group (mean difference 67.19 mm 3, P=0.049); on the 14th day after the start of treatment, the tumor volume in the tetramer treatment group was significantly smaller than that in the monomer treatment group (mean difference 414.33 mm 3, P=0.005). Conclusion:FAPI-tetramer can improve tumor uptake and retention ability compared with FAPI-46, and 177Lu-DOTA-4P(FAPI) 4 can be a promising radio-agent for FAP-positive tumor therapy.

9.
Chinese Journal of Lung Cancer ; (12): 119-134, 2023.
Article in Chinese | WPRIM | ID: wpr-971187

ABSTRACT

BACKGROUND@#The incidence and mortality of lung cancer have always been at the forefront of malignant tumors. With the development of lung cancer detection techniques, more peripheral pulmonary lesions (PPLs) have been detected. The diagnostic accuracy of procedures for PPLs keeps controversial. This study aims to systematically evaluate the diagnostic value and the safety of electromagnetic navigation bronchoscopy (ENB) in the diagnosis of PPLs.@*METHODS@#The relevant literatures in the diagnostic yield of PPLs by ENB were systematically retrieved from Wanfang Data Knowledge Service Platform, China National Knowledge Infrastructure, Embase, PubMed, Cochrane Library and Web of Science. The software of Stata 16.0, RevMan 5.4 and Meta-disc 1.4 were used to conduct the meta-analysis.@*RESULTS@#A total of 54 literatures with 55 studies were included in our meta-analysis. The pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and diagnostic odds ratio of ENB in the diagnosis of PPLs were 0.77 (95%CI: 0.73-0.81), 0.97 (95%CI: 0.93-0.99), 24.27 (95%CI: 10.21-57.67), 0.23 (95%CI: 0.19-0.28) and 104.19 (95%CI: 41.85-259.37), respectively. The area under curve (AUC) was 0.90 (95%CI: 0.87-0.92). Meta-regression and subgroup analyses indicated that the potential heterogeneity resulted from study type, additional localization techniques, sample size, lesion size and type of sedation. The use of additional localization techniques and general anesthesia have improved the diagnostic efficiency of ENB in PPLs. The incidence of adverse reactions and complications associated with ENB was very low.@*CONCLUSIONS@#ENB provides well diagnostic accuracy and safety.


Subject(s)
Humans , Bronchoscopy , Lung Neoplasms , Anesthesia , China , Electromagnetic Phenomena
10.
Chinese Acupuncture & Moxibustion ; (12): 721-726, 2023.
Article in Chinese | WPRIM | ID: wpr-980785

ABSTRACT

A multifunctional moxibustion treatment machine is designed and developed to assist the heat-sensitive moxibustion therapy. Through the motion control of the stepping motor by programmable logic controller (PLC), the automatic control is obtained for the acupoint detection of heat-sensitive moxibustion therapy and the manual operation of moxibustion. The skin temperature is monitored in real-time, using infrared non-contact temperature measurement technology. Based on the deviation of the temperature set value and the monitoring one, the distance between the moxibustion device and the exerted region is adjusted automatically by PLC so that the temperature is controlled practically. The multifunctional moxibustion treatment machine based on the heat-sensitive moxibustion therapy is capable of the operation control of mild moxibustion, circling moxibustion, sparrow-pecking moxibustion and along-meridian moxibustion techniques, as well as real-time monitoring of skin temperature. The temperature change curve of this machine is coincident with that obtained by the manual operation of heat-sensitive moxibustion. This multifunctional moxibustion treatment machine assists the delivery of heat-sensitive moxibustion therapy and it is satisfactory in temperature control and precise in operation.


Subject(s)
Hot Temperature , Moxibustion , Pain Management , Acupuncture Points , Meridians
11.
Acta Pharmaceutica Sinica ; (12): 1770-1779, 2023.
Article in Chinese | WPRIM | ID: wpr-978650

ABSTRACT

On the basis of the qualitative preparation quality markers of Zhibao Sanbian Wan (ZBSBW), we screened out the quantitative markers and evaluated the content consistency of ZBSBW. A method capable of simultaneously determining 34 compounds in ZBSBW was established based on HPLC-MS/MS, and 16 batches of ZBSBW were simultaneously analyzed by this method. Furthermore, we explored a general strategy for analyzing the component migration in preparation, plasma, brain tissue and cerebrospinal fluid. The methodological investigation was confirmed by linear range, recovery (85.10%-105.07%), precision (RSD: 1.37%-4.58%), stability, and repeatability (3.00%-12.45%), the established method was suitable for the detection and quantification of the compounds in ZBSBW. The contents of compounds in ZBSBW were all lower than 1 mg·g-1, and the contents and daily dose of nystose were the highest, followed by echinacoside, paeoniflorin, osthole and paeonol. The results of systematic clustering showed that the contents were consistent for ordinary preparations of ZBSBW. The principal component analysis showed that the components of berberine, ginsenoside Re, ginsenoside Rg1, pinoresinol diglucoside and tenuifolin had large variation, which contributed significantly to the grouping. The contents of echinacoside, verbascoside, polygalaxanthone Ⅲ, β-ecdysterone, osthole, alisol B 23-acetate, liquiritin and glycyrrhizic acid were stable from batch to batch. The animal experiment results showed that osthole, paeonol and liquiritin in ZBSBW could be absorbed into the blood and enter the brain tissue by passing through the blood-brain barrier. All animal studies were reviewed and approved by the Institutional Animal Care and Use Committee at Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences (No. 2020B071). The above compounds contributed the quantitative preparation quality markers of ZBSBW. In conclusion, the HPLC-MS/MS method established in this study was sensitive, accurate and rapid, and could be used for simultaneous quantification of 34 compounds and content consistency evaluation of multiple batches of preparations in ZBSBW. The result provided a methodological basis for the screening of quantitative preparation quality markers and material basis research of ZBSBW.

12.
International Eye Science ; (12): 1390-1394, 2023.
Article in Chinese | WPRIM | ID: wpr-978640

ABSTRACT

AIM: To evaluate the damage to optic nerve by detecting the morphological and functional changes of optic nerve in patients with acute primary angle-closure glaucoma.METHODS: The correlation among the positive rates of fundus photography, visual field examination, optical coherence tomography(OCT)scan of the optic nerve, and pattern visual evoked potentials(P-VEP)were analyzed in 28 patients with acute primary angle-closure glaucoma affecting.RESULTS: Among 28 eyes of 28 patients, optic nerve images were obtained in 25 eyes(89%), optic disc color in 25 eyes: there were 9 normal eyes and 16 eyes with congestion and edema, without pallor; C/D: &#x003C; 0.6 in 5 eyes, &#x003E; 0.6 in 1 eye, and indistinguishable in 19 eyes; disk edge: 6 eyes were normal, and 19 eyes could not be distinguished.Optic nerve images could not be obtained in 3 eyes(11%). Visual field examination: 9 eyes(32%)were normal, 2 eyes(7%)had visual field damage, 8 eyes(29%)had no reference value due to cataract visual field examination, 2 eyes(7%)had false positive rate ≥15%, and 7 eyes(25%)could not cooperate with the examination. The retinal nerve fiber layer thickness around optic disc was measured by OCT in 26 eyes(93%), of which the thickness of nerve fiber layer was normal in 24 eyes and local thinning in 2 eyes. Severe opacification of refractive stroma could not be measured in 2 eyes(7%). PVEP showed that the peak of P100 was normal in 18 eyes(64%)and prolonged in 10 eyes(36%). The amplitude of P100 wave was normal in 11 eyes(39%)and decreased in 17 eyes(61%).CONCLUSION: After a major acute attack of primary angle-closure glaucoma, the optic nerve does not exhibit typical glaucomatous damage but may present with optic disc congestion and edema. Therefore, a comprehensive evaluation combining subjective and objective examination methods is necessary for optic nerve assessment, and currently, no more precise assessment method is available.

13.
Chinese Journal of Applied Clinical Pediatrics ; (24): 281-285, 2023.
Article in Chinese | WPRIM | ID: wpr-990026

ABSTRACT

Objective:To investigate the value of bronchoalveolar lavage fluid (BALF) genechip analysis for the identification of pathogens in children with refractory pneumonia.Methods:A retrospective study of 500 children clinically diagnosed with refractory pneumonia in the Department of Respiratory and Critical Care Medicine, Kunming Children′s Hospital, Kunming Medical University between January 2020 to January 2022 was made.During hospitalization, bronchoscopic examination and bronchoalveolar lavage were performed.BALF was collected and analyzed using genechip technology to detect potential pathogens.At the same time, bacterial culture tests of sputum and BALF samples from the patients were performed. χ2 test was used to compare the positive rates of pathogens detected by different detection methods. Results:Of the 500 children patients, 482 cases (96.4%) were positive of BALF genechip analysis for pathogen identification.There were 71 cases (14.7%) infected with a single pathogen, and 411 cases (85.3%) with 2 or more pathogens.The top 3 bacteria were Streptococcus pneumoniae [117 cases (8.3%)], Haemophilus influenzae [63 cases (4.5%)], and Bordetella pertussis [32 cases (2.3%)]. The patients were mostly infected with respiratory syncytial virus [269 cases (19.1%)], followed by parainfluenza virus [217 cases (15.4%)], and adenovirus [132 cases (9.3%)]. Among the 500 patients, 116 cases (23.2%) were positive of BALF genechip analysis for bacteria identification, 47 cases (9.4%) had a positive BALF culture, 43 cases (8.6%) had a positive sputum culture.The bacterial detection rate of BALF genechip analysis was statistically significantly higher than that of BALF culture and sputum culture tests ( χ2=34.90, 39.85; all P<0.001). Conclusions:Most patients with refractory pneumonia have mixed infections.The genechip technology can rapidly and efficiently identify the pathogens, thus providing clinical guidance for anti-infection treatment.

14.
Chinese Journal of Hepatology ; (12): 49-55, 2023.
Article in Chinese | WPRIM | ID: wpr-970951

ABSTRACT

Objective: To understand the basic characteristics of previously reported patients with hepatitis C and analyze the related factors affecting their antiviral treatment. Methods: A convenient sampling method was adopted. Patients who had been previously diagnosed with hepatitis C in the Wenshan Prefecture of Yunnan Province and Xuzhou City of Jiangsu Province were contacted by telephone for an interview study. The Andersen health service utilization behavior model and related literature were used to design the research framework for antiviral treatment in previously reported hepatitis C patients. A step-by-step multivariate regression analysis was used in previously reported hepatitis C patients treated with antiviral therapy. Results: A total of 483 hepatitis C patients, aged 51.73 ± 12.06 years, were investigated. The proportion of male, agricultural occupants who were registered permanent residents, farmers and migrant workers was 65.24%, 67.49%, and 58.18%, respectively. Han ethnicity (70.81%), married (77.02%), and junior high school and below educational level (82.61%) were the main ones. Multivariate logistic regression analysis results showed that married patients with hepatitis C (OR = 3.19, 95% CI: 1.93-5.25, compared with unmarried, divorced, and widowed patients) with high school education or above (OR = 2.54, 95% CI: 1.54-4.20, compared with patients with junior high school education or below) were more likely to receive antiviral treatment in the predisposition module. Patients with severe self-perceived hepatitis C in the need factor module (compared with patients with mild self-perceived disease, OR = 3.36, 95% CI: 2.09-5.40) were more likely to receive treatment. In the competency module, the family's per capita monthly income was more than 1,000 yuan (compared with patients with per capita monthly income below 1,000 yuan, OR = 1.59, 95% CI: 1.02-2.47), and the patients had a high level of awareness of hepatitis C knowledge (compared with patients with a low level of knowledge, OR = 1.54, 95% CI: 1.01-2.35), and the family members who knew the patient's infection status (compared with patients with an unknown infection status, OR = 4.59, 95% CI: 2.24-9.39) were more likely to receive antiviral treatment. Conclusion: Different income, educational, and marital statuses are related to antiviral treatment behavior in hepatitis C patients. Family support of hepatitis C patients receiving hepatitis C-related knowledge and their families knowing the infection status is more important in promoting the antiviral treatment of patients, suggesting that in the future, we should further strengthen the hepatitis C knowledge of hepatitis C patients, especially the family support of hepatitis C patients' families in treatment.


Subject(s)
Humans , Male , Antiviral Agents/therapeutic use , China , Hepatitis C/drug therapy , Hepacivirus , Logistic Models
15.
Chinese Journal of Biologicals ; (12): 11-15+20, 2023.
Article in Chinese | WPRIM | ID: wpr-965559

ABSTRACT

Objective@#To evaluate the inhibitory effect of tumor vaccines in colon carcinoma model mice.@*Methods@# Mouse bone marrow⁃derived dendritic cells(BMDCs)were stimulated by using CpG β⁃glucan nanoparticles(CNP)in vitro. The BMDCs were divided into PBS group,NP group(without CpG nanoparticles),Lysate group(MC38 cell lysate)and CpG group(CpG1826),which were determined for the expression of marker molecules on the surface by flow cytometry and for the contents of interleukin⁃6(IL⁃6)and IL⁃12p40 in the culture supernatant by ELISA. The tumor lysate nano⁃vaccine was pre⁃ pared by mixing 50 mg/mL tumor lysate(MC38 cell lysate)with 200 mg/mL CNP in a volume ratio of 1∶1,with which mice were subcutaneously immunized as Vaccine group. Vaccine group,PBS group,CNP group and Lysate group were im⁃ munized once a week,for three times in total. Mice were subcutaneously inoculated with MC38 cells,2 × 105 cells for each, in the right lower limb 1 h after the last immunization,and measured for tumor volume once every three days to plot the tumor growth curve. The ratios of CD3+ CD4+ T and CD3+ CD8+ T cells in the blood were analyzed by flow cytometry and the levels of tumor necrosis factor⁃α(TNF⁃α)and interferon γ(IFNγ)in the blood and spleen of mice were determined by ELISA.@*Results@# CNP effectively increased the expression of CD11c+ CD80+,CD11c+ CD86+,CD11c+ MHC⁃Ⅱ+ and the secretion of IL⁃6 and IL⁃12p40 in BMDCs in vitro,which were significantly higher than those in other 4 groups(t = 4. 3 ~ 46. 2,each P < 0. 05). Compared with that of the other three groups,the tumor volume of mice in Vaccine group decreased significantly(t =2.6~3.4,eachP <0. 05);TherewasnosignificantdifferenceinCD3+ CD8+ TandCD3+ CD8+ Tcellratios(t = 0.5~ 1. 9,each P > 0. 05);The content of IFNγ in blood increased significantly(t = 3. 8 ~ 4. 6,P < 0. 05),while thatof TNF⁃α showed no significant difference(t = 0. 4 ~ 2. 0,each P > 0. 05);However,the contents of IFN γ and TNF⁃α in spleen increased significantly(t = 6. 3 ~ 13. 0,each P < 0. 001).@*Conclusion@#The prepared nano⁃vaccine of tumor lysate improvedtheimmune level in mice and effectively inhibited the growth of colon carcinoma.

16.
Chinese Journal of Traumatology ; (6): 27-31, 2022.
Article in English | WPRIM | ID: wpr-922356

ABSTRACT

PURPOSE@#To investigate the clinical value of urine interleukin-18 (IL-8), neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) for the early diagnosis of acute kidney injury (AKI) in patients with ureteroscopic lithotripsy (URL) related urosepsis.@*METHODS@#A retrospective study was carried out in 157 patients with urosepsis after URL. The patients were divided into AKI group and non-AKI group according to the Kidigo guideline and urine IL-8, NGAL and KIM-1 levels were detected by enzyme-linked immunosorbent assay at 0, 4, 12, 24 and 48 h after the surgery. Receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of these three biomarkers for postoperative AKI.@*RESULTS@#The level of urine IL-8, NGAL and KIM-1 in AKI group was significantly higher than that in non-AKI group at 4, 12, 24 and 48 h (p < 0.01). The ROC analysis showed the combined detection of urine IL-8, NGAL and KIM-1 at 12 h had a larger area under curve (AUC) than a single marker (0.997, 95% CI: 0.991-0.998), and the sensitivity and specificity were 98.2% and 96.7%, respectively. Pearson correlation analysis showed that the levels of urine NGAL at 4, 12, 24 and 48 h in AKI patients were positively correlated with the levels of urine KIM-1 and IL-18 (p < 0.01).@*CONCLUSION@#AKI could be quickly recognized by the elevated level of urine IL-8, NGAL and KIM-1 in patients with URL-related urosepsis. Combined detection of the three urine biomarkers at 12 h after surgery had a better diagnostic performance, which may be an important reference for the early diagnosis of AKI.


Subject(s)
Humans , Acute Kidney Injury/etiology , Biomarkers , Early Diagnosis , Hepatitis A Virus Cellular Receptor 1 , Interleukin-18 , Interleukin-8 , Lipocalin-2 , Lithotripsy , Retrospective Studies , Ureteroscopy
17.
Chinese Journal of Cardiology ; (12): 585-590, 2022.
Article in Chinese | WPRIM | ID: wpr-940892

ABSTRACT

Objective: We evaluated the safety and efficacy of lipoprotein apheresis (LA) in patients with familial hypercholesterolemia (FH) who can't reach low-density lipoprotein cholesterol(LDL-C) target goals with the maximal tolerated dose of lipid-lowering agents. Methods: This was a retrospective cross-sectional study. Between February 2015 and November 2019, patients with FH who were admitted in Fuwai hospital and treated with LA were consecutively enrolled. Based on intensive lipid-lowering agents, these patients received LA by double filtration plasma pheresis (DFPP) method. The changes of lipid levels such as LDL-C and lipoprotein(a)[Lp(a)] were compared before and after LA treatment, and the changes of immunoglobulin (Ig) concentration and LA-related adverse effects were also discussed. Results: A total of 115 patients with FH were enrolled in this study, of which 8 cases were homozygous FH and 107 cases were heterozygous FH. The age was (43.9±12.2) years and there were 75 (65.2%) males, and 108 (93.8%) with coronary artery disease. For pre-and immediately after LA treatment, the LDL-C was (5.20±2.94) mmol/L vs. (1.83±1.08) mmol/L, Lp(a) concentration was 428.70(177.00, 829.50)mg/L vs. 148.90(75.90, 317.00) mg/L (P<0.001), with a decrease of 64.2% and 59.8% respectively. The levels of IgG and IgA measured 1 day after LA treatment were both in the normal range and IgM concentration was below the reference value, the reductions of which were 15.1%, 25.0% and 58.7% respectively (P<0.001). Six patients had mild symptoms of nausea, hypotension dyspnea and palpitation, the symptoms were relieved by symptomatic treatment. Conclusion: For patients with FH who do not achieve LDL-C target goal with the maximal tolerated lipid-lowering agents, especially those with elevated Lp(a) levels, LA, which can significantly further reduce LDL-C and Lp(a) levels, is an effective and safe option.


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Blood Component Removal/methods , Cholesterol, LDL , Cross-Sectional Studies , Hyperlipoproteinemia Type II/therapy , Lipoprotein(a)/chemistry , Lipoproteins/chemistry , Retrospective Studies
18.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 100-108, 2022.
Article in Chinese | WPRIM | ID: wpr-940766

ABSTRACT

ObjectiveTo explore the effect of Yifei Huatan decoction on relieving airway hyperviscosity in asthmatic rats with spleen deficiency syndrome and its mechanism. MethodFifty-five SPF level SD rats at 8-9 week of age were used to induce asthma with spleen deficiency syndrome by animal modeling of traditional Chinese medicine combined with asthma of western medicine. After successful modeling, the rats were divided into model group, dexamethasone group, low, medium, and high-dose Yifei Huatan decoction groups by random number table method, and 11 clean SD rats at 8-9 week of age were recorded as a normal group. Rats in the dexamethasone group were given 0.087 5 mg kg-1 dexamethasone acetate by gavage. Rats in the low, medium, and high-dose Yifei Huatan decoction groups were given 0.8, 1.6, 3.2 g kg-1 Yifei Huatan decoction liquid extract by gavage, respectively. Rats in the model group and the normal group were given 10 mL kg-1 distilled water. The medicine were given once per day for 8 w, and the general situation of each group was observed. The levels of interleukin-4 (IL-4), IL-13, and interferon-γ (IFN-γ) in bronchoalveolar lavage fluid (BALF) were determined by enzyme-linked immunosorbent assay (ELISA). The pathological changes in lung tissues of rats were observed by hematoxylin-eosin (HE) staining. Alcian blue-periodic acid Schiff (AB-PAS) staining was used to detect the hyperplasia of airway goblet cells and mucus secretion in rats. The mRNA expressions of transforming growth factor-β1 (TGF-β1), Smad2, Smad3, mucin 5AC (MUC5AC), and mucin 5B (MUC5B) in the lung tissues of rats were detected by real-time quantitative polymerase chain reaction (Real-time PCR). The protein expressions of TGF-β1, Smad2, Smad3, MUC5AC, and MUC5B in the lung tissues of rats were detected by Western blot. ResultAs compared with the normal group, rats in the model group showed the symptoms of spleen deficiency syndrome, such as decreased body weight, muscle emaciation, decreased food intake, increased water intake, increased anal temperature, tiredness, and decreased swimming endurance, accompanied by dyspnea symptoms such as wheezing and nodding. As compared with the normal group, IL-4 and IL-13 levels in the BALF of the model group were significantly increased (P<0.01), while the IFN-γ level was significantly decreased (P<0.01). In the model group, a large number of inflammatory cells were observed in the mucosa and submucosa of the airway, and the smooth muscle of the trachea was significantly thickened. The hyperplasia, deformation, and exfoliation of various epithelial cells were observed in the mucosa, and the pathological scores of lung tissue increased significantly (P<0.01) in the model group. A large number of goblet cells were observed in the airway with the formation of plenty of mucous thrombus in the model group, and the positive relative staining area of airway, and mRNA and protein expressions of TGF-β1, Smad2, Smad3, MUC5AC, and MUC5B were significantly increased (P<0.01). As compared with the model group, IL-4 and IL-13 levels in BALF of the dexamethasone group and the Yifei Huatan decoction groups decreased, while the IFN-γ level increased. The inflammatory cell infiltration in airway mucosa and submucosa, the thickening of tracheal smooth muscle, the hyperplasia, deformation, and exfoliation of epithelial cells in mucosa were gradually decreased, and the pathological scores of lung tissues decreased significantly (P<0.01) in the dexamethasone group and the Yifei Huatan decoction groups. Goblet cell proliferation gradually decreased, and the positive relative staining area of airway, and mRNA and protein relative expressions of TGF-β1, Smad2, Smad3, MUC5AC, and MUC5B decreased with statistically significant difference (P<0.05, P<0.01). There was no significant difference in the above indexes in the dexamethasone group and the Yifei Huatan decoction low-dose group. The above indexes were dose-dependent in the low, medium, and high-dose Yifei Huatan decoction groups. ConclusionYifei Huatan decoction reduces airway hyperviscosity in asthmatic rats with spleen deficiency syndrome, which may be related to the inhibition of TGF-β1, Smad2, Smad3, MUC5AC, and MUC5B expressions, down-regulation of IL-4 and IL-13 levels, and up-regulation of IFN-γ level.

19.
Chinese Journal of Preventive Medicine ; (12): 212-217, 2022.
Article in Chinese | WPRIM | ID: wpr-935271

ABSTRACT

The evaluation methods of immune persistence include direct evaluation, indirect evaluation, model prediction, and meta-analysis and so on. Direct evaluation is the gold standard for evaluating the immune persistence of vaccines by quantifying the protective effect of vaccines on the onset and (or) infection of preventable diseases. Indirect evaluation of immune persistence by immunological surrogate indicators is more widely used in practice. In addition, mathematical models and meta-analysis can also be used to evaluate the immune persistence of vaccines. It is of great significance to select the appropriate evaluation method to analyze the immune persistence of the vaccine according to the specific situation.


Subject(s)
Humans , Immunity , Meta-Analysis as Topic , Vaccines/immunology
20.
Acta Pharmaceutica Sinica ; (12): 3494-3501, 2022.
Article in Chinese | WPRIM | ID: wpr-964324

ABSTRACT

Depression is a common emotional mental disorder. Patients not only continuously showed depression, pessimism and apathy in mood, but also have gastrointestinal symptoms such as anorexia and constipation in body. Widely attention has been also received in the potential biological role of gut microbiota in the pathogenesis of depression. It plays an important role in the interaction between the intestine and the brain, not only affecting the intestinal barrier function, but also maintaining the homeostasis of host through the microbiota-gut-brain axis. In recent years, the traditional Chinese medicine (TCM) has the advantages of obvious therapeutic effects and few side effects when treating neuropsychiatric diseases, such as depression. The pharmacological mechanism of TCM exerting antidepressant effects by regulating the structure of gut microbiota, reducing displacement, and maintaining the normal function of gut microbiota has been also widely concerned. By investigating the relevant literature in recent years, this paper summarizes the antidepressant effect of TCM in different directions such as Chinese medicine monomer, single medicine and compound medicine. And this paper reviews the antidepressant effects and mechanisms of TCM at different levels, such as the correction of gut microbiota structure, the regulation of immunity, the transplantation of gut microbiota and the regulation of its metabolites. This paper will provide a basis for further explaining the mechanism of gut microbiota in depression and the mechanism of antidepressant effect of TCM.

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