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1.
Article in Chinese | WPRIM | ID: wpr-879151

ABSTRACT

The paclitaxel-loaded and folic acid-modified poly(lactic-co-glycolic acid) nano-micelles(PTX@FA-PLGA-NMs) were prepared by the emulsion solvent evaporation method, and the parameters of paclitaxel-loaded nano-micelles were optimized with the particle size and PDI as evaluation indexes. The morphology of the nano-micelles was observed by transmission electron microscopy(TEM), and the stability, drug loading and encapsulation efficiency were systematically investigated. In vitro experiments were performed to study the cytotoxic effects of nano-micelles, apoptosis, and cellular uptake. Under the optimal parameters, the nano-micelles showed the particle size of(125.3±1.2) nm, the PDI of 0.086±0.026, the zeta potential of(-20.0±3.8) mV, the drug loading of 7.2%±0.75%, and the encapsulation efficiency of 50.7%±1.0%. The nano-micelles were in regular spherical shape as observed by TEM. The blank FA-PLGA-NMs exhibited almost no inhibitory effect on the proliferation and growth of tumor cells, while the drug-loaded nano-micelles and free PTX exhibited significant inhibitory effects. The IC_(50) of PTX@FA-PLGA-NMs and PTX was 0.56 μg·mL~(-1) and 0.66 μg·mL~(-1), respectively. The paclitaxel-loaded nano-micelles were potent in inhibiting cell migration as assessed by the scratch assay. PTX@FA-PLGA-NMs had good pro-apoptotic effect on cervical cancer HeLa cells and significantly promoted the uptake of HeLa cells. The results of in vitro experiments suggested that PTX@FA-PLGA-NMs could target and treat cervical cancer HeLa cells. Therefore, as nanodrug carriers, PTX@FA-PLGA-NMs with anti-cancer activity are a promising nano-system for improving the-rapeutic effects on tumors.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Cell Line, Tumor , Drug Carriers , Female , Folic Acid , Glycolates , HeLa Cells , Humans , Micelles , Paclitaxel , Particle Size , Uterine Cervical Neoplasms/drug therapy
2.
Article in Chinese | WPRIM | ID: wpr-878676

ABSTRACT

Objective To investigate the short-term outcome of T1bN0M0 papillary thyroid cancer after ultrasonography-guided radiofrequency ablation(RFA). Methods Eighty-nine patients with T1bN0M0 papillary thyroid cancer who were treated with ultrasonography-guided RFA in our center from April 2014 to January 2019 were retrospectively analyzed.Trans-isthmus approach and moving shot technique were used during the RFA procedure.Ultrasonography and contrast-enhanced ultrasonography were performed before ablation,and immediately,1,3,6 and 12 months after ablation and then every 6 months thereafter. Results RFA was performed in 89 cases of papillary thyroid cancer,and no major complications were observed during the RFA.The mean follow-up was(18.8±7.3)months.The ablation zones decreased gradually during follow-up,and 38 ablation zones(42.7%)completely disappeared.The volume reduction rate was(99.2±2.3)% 30 months after ablation.During follow-up,2 patients(2.2%)developed tumor recurrence and 1 patient(1.1%)developed cervical lymph node metastasis. Conclusion Ultrasonography-guided RFA may be a safe and effective method for patients with T1bN0M0 papillary thyroid cancer.


Subject(s)
Catheter Ablation , Humans , Lymphatic Metastasis , Neoplasm Recurrence, Local , Radiofrequency Ablation , Retrospective Studies , Thyroid Cancer, Papillary/surgery , Thyroid Neoplasms/surgery , Treatment Outcome , Ultrasonography
3.
Article in Chinese | WPRIM | ID: wpr-826360

ABSTRACT

To investigate the value of injecting a small amount of absolute ethanol into the benign solid nodules of the thyroid before radiofrequency ablation(RFA)to improve the efficiency of radiofrequency ablation. A total of 98 eligible patients(98 nodules)with pathologically confirmed benign solid nodules who were treated in our center from December 2016 to February 2018 were included and randomized into ethanol ablation(EA)combined with radiofrequency ablation(RFA)group(EA+RFA group)and RFA group,with 49 patients in each group.Routine ultrasound,contrast-enhanced ultrasound(CEUS),and thyroid function test were performed before treatment and 1,3,6,and 12 months after treatment.The general information,treatment time,ablation energy,ablation power,postoperative nodule volume reduction ratio(VRR),symptom score(SS)and cosmetic score(CS),thyroid function level,and incidence of complications were compared between these two groups. The mean treatment time [(441.30±243.31)s (790.70±349.82)s;= 4.403, =0.000],mean ablation energy [(3.92±2.01)kJ (5.15±2.12)kJ;=2.709, =0.009],and mean ablation power [(6.07±1.44)W (7.30±1.29)W;=3.612, =0.006] were significantly lower in the EA+RFA group than in the RFA group.At 3,6 and 12 months after surgery,the VRR in the EA+RFA group was(57.73±11.07)%(=-3.16, <0.001),(64.40±10.56)%(=-5.45, <0.001),and(77.29±8.48)%(=-10.46, <0.001),respectively;the VRR in the RFA group was(55.44±13.01)%(=-1.76, <0.001),(65.28±11.33)%(=-5.09, <0.001),and(75.17±9.84)%(=-8.93, <0.001),which were significantly smaller than those before surgery.There was no significant difference in VRR between the EA+RFA group and the RFA group at 1(=3.41, =0.33),3(=2.05, =0.21),6(=2.77, =0.49),and 12 months(=5.05, =0.10)after treatment.During the follow-up,no recurrence of nodules was observed on CEUS.In the EA+RFA group,the SS [(1.77±0.86).(5.54±2.15);=9.63, <0.001] and the CS[(1.39±0.77).(3.32±0.61);=10.09, =0.004]at 12 months after surgery were significantly lower than those before surgery.In the RFA group,SS [(1.63±1.04).(5.90±1.79);=12.72, <0.001] and CS [(1.64±0.83).(3.15±0.72);=8.13, =0.012] at 12 months after surgery were also significantly lower than those before surgery.The CSS in the EA+RFA group was significantly lower than that in the RFA group [(0.93±0.55).(2.44±0.53);=-11.70, =0.007].Both groups had no significant change in thyroid function during the follow-up period,and no serious complications were observed. Anhydrous alcohol injection can effectively improve the efficiency of radiofrequency ablation in treating benign solid thyroid nodules and is effective in reducing nodule volume,alleviating compressive symptoms,and decreasing cosmetic discomfort.


Subject(s)
Catheter Ablation , Ethanol , Humans , Neoplasm Recurrence, Local , Prospective Studies , Thyroid Nodule , Treatment Outcome
4.
Article in Chinese | WPRIM | ID: wpr-868805

ABSTRACT

Objective:To investigate the effects of estrogen receptor beta agonist diarylpropionitrile (DPN) on mesenteric artery (MA) responsiveness and on Rho-kinase signaling pathway in cirrhotic portal hypertension rats, and to elucidate the mechanism of ER beta agonist in visceral hyperdynamic circulation in cirrhotic portal hypertension rats.Methods:Female rats were treated with bilateral ovariectomy, and CCl 4 was injected to establish cirrhotic portal hypertension model. After intervention, hemodynamic parameters and vascular reactivity of MA in each group were measured, and the levelof ER beta, Rho-kinase signaling pathway-related proteins and receptor desensitization-related proteins in MA in each group were detected. Results:DPN could significantly improve the hemodynamic parameters of ovariectomized cirrhosis rats and the response of MA to norepinephrine. DPN could increase the level of inhibited ROCK protein and decrease the expression of beta-arrestin-2 and the phosphorylation of ERK1/2.Conclusions:DPN could improve the responsiveness of MA to vasoconstrictor substances, and significantly improve the hyperdynamic circulation in ovariectomized cirrhosis rats. This effect may be related to DPN improving the desensitization of MA to vasoconstrictor receptors and the Rho-kinase pathway activation.

5.
Article in Chinese | WPRIM | ID: wpr-837848

ABSTRACT

Objective To investigate the expression of microfibril-associated protein 5 (MFAP5) in bladder cancer tissues and its biological effects on bladder cancer cells. Methods The surgical resection specimens of bladder cancer patients, who were diagnosed in Shanghai General Hospital of Shanghai Jiao Tong University, were collected, and the expression level of MFAP5 was detected using immunohistochemistry. The cancer-associated fibroblasts (CAFs) and normal fibroblasts (NFs) were isolated and purified from bladder tumor tissues and normal bladder tissues, respectively. The mRNA expression levels of MFAP5 in CAFs, NFs, and four kinds of human bladder tumor cell lines (T24, 5637, UMUC3 and J82) were detected using quantitative real-time polymerase chain reaction. The correlation of MFAP5 expression with the progression and malignancy of bladder cancer was analyzed using the bladder cancer expression profile data derived from the Cancer Genome Atlas (TCGA) database and the GSE13507 database of Gene Expression Omnibus (GEO). The T24 and 5637cells were treated with recombinant human MFAP5 protein to detect the effect on cell migration and invasion, as well as the expression of migration-associated proteins. Results MFAP5 was mainly expressed in the stroma of bladder cancer tissues, and its expression level was related to T stage and tumor grade of bladder cancer (both P<0.05). The mRNA expression level of MFAP5 in CAFs was significantly higher than those in NFs, and T24, 5637, UMUC3 and J82 cells (all P<0.01). The results of TCGA and GSE13507 database both showed that the overall survival of bladder cancer patients with high expression of MFAP5 was significantly shorter than that of patients with low expression of MFAP5 (both P<0.05). Gene set variation analysis showed that the expression level of MFAP5 increased with the degree of tumor malignancy (P<0.01), and its expression was closely related to expression of epithelial-mesenchymal transition-related genes such as cadherin 2, Twist1, matrix metalloproteinase 9 (MMP9), and zinc-finger E-box-binding protein 1 (ZEB1) (all P<0.01). In T24 and 5637 cells, the stimulation of recombinant human MFAP5 protein could up-regulate the expression of epithelial-mesenchymal transitionrelated proteins, including N-cadherin, MMP9, ZEB1 and Twist, and enhance the migration and invasion abilities (all P<0.01). Conclusion MFAP5 may be a potential molecular marker for predicitng the invasion and progression of bladder cancer.

6.
Article in Chinese | WPRIM | ID: wpr-872934

ABSTRACT

Objective:To predict the anti-inflammatory targets and relevant signaling pathways of Epimedii Folium in the treatment of depression by network pharmacology,in order to explore the potential mechanism of its anti-depression effect. Method:The active constituents of Epimedii Folium were collected and screened out through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) database. PharmMapper server and TCMSP database were used to predict and screen out protein targets. OMIM database,CTD database and GeneCards database were used to screen out relevant targets and anti-inflammatory targets of depression. Enrichment analysis of the gene ontology (GO) function and Kyoto Encyclopedia of genes and genomes(KEGG) signaling pathway for the key anti-inflammatory targets of Epimedii Folium were carried out by DAVID database. Cytoscape 3.6.0 was used to construct the network diagram of "active component-action target-signal pathway" of Epimedii Folium and analyze the topological structure of the network. GOLD molecular docking software was used to verify the results of active components and key anti-inflammatory targets. Result:A total of 12 active components,30 targets and 5 key anti-inflammatory targets of Epimedii Folium were screened out, 65 biological processes,4 cell components and 1 molecular function were enriched with GO function, and 41 KEGG pathways were enriched and analyzed,including 9 inflammation-related signaling pathways. Molecular docking verified that icariin and key anti-inflammatory targets could form the optimal binding structure. Conclusion:The study preliminarily reveals the molecular mechanism of Epimedii Folium on depression through its anti-inflammatory target and its relevant signaling pathway network,so as to provide a basis for further study on the antidepressant effect of Epimedii Folium.

7.
Article in English | WPRIM | ID: wpr-776869

ABSTRACT

Isoflavones are widely consumed by people around the world in the form of soy products, dietary supplements and drugs. Many isoflavones or related crude extracts have been reported to exert pain-relief activities, but the mechanism remains unclear. Voltage-gated sodium channels (VGSCs) play important roles in excitability of pain sensing neurons and many of them are important nociceptors. Here, we report that several isoflavones including 3'-methoxydaidzein (3MOD), genistein (GEN) and daidzein (DAI) show abilities to block VGSCs and thus to attenuate chemicals and heat induced acute pain or chronic constriction injury (CCI) induced pain hypersensitivity in mice. Especially, 3MOD shows strong analgesic potential without inducing addiction through inhibiting subtypes Na1.7, Na1.8 and Na1.3 with the IC of 181 ± 14, 397 ± 26, and 505 ± 46 nmol·L, respectively, providing a promising compound or parent structure for the treatment of pain pathologies. This study reveals a pain-alleviating mechanism of dietary isoflavones and may provide a convenient avenue to alleviate pain.


Subject(s)
Analgesics , Chemistry , Animals , Humans , Isoflavones , Chemistry , Male , Mice , Mice, Inbred C57BL , Pain , Drug Therapy , Genetics , Metabolism , Voltage-Gated Sodium Channel Blockers , Voltage-Gated Sodium Channels , Genetics , Metabolism
8.
Journal of Experimental Hematology ; (6): 1118-1122, 2019.
Article in Chinese | WPRIM | ID: wpr-775755

ABSTRACT

OBJECTIVE@#To explore the significance of lymphocyte to monocyte ratio (LMR) in the disease progress of primary gastrointestinal diffuse large B-cell lymphoma (PGI-DLBCL).@*METHODS@#The clinical data of 43 patients diagnosed as PGI-DLBCL in our hospital from January 2011 to December 2015 were collected, and the disease progress was followed up.@*RESULTS@#According to the ROC curve, the threshold value of LMR for 2 years PFS (%) of PGI-DLBCL patients was 2.6. Unvariate analysis showed that LMR (P<0.05), large enclosed mass lesion (P<0.01) and IPI (P<0.05) were prognostic factors affecting PFS, the COX regression model multivariate analysis showed that LMR<2.6 [ (risk ratio (RR)=3.083, 95%CI 1.828-8.313, P<0.01], and large enclosed mass lesions (RR=2.718, 95%CI 1.339-6.424, P<0.05) were the independent adverse prognostic factor for two years PFS.@*CONCLUSION@#Both LMR<2.6 and large enclosed mass lesions relate with the progress of PGI-DLBCL.


Subject(s)
Humans , Leukocyte Count , Lymphocytes , Lymphoma, Large B-Cell, Diffuse , Monocytes , Prognosis , Retrospective Studies
9.
Journal of Experimental Hematology ; (6): 1482-1489, 2019.
Article in Chinese | WPRIM | ID: wpr-775695

ABSTRACT

OBJECTIVE@#To investigate the effects and its potential mechanism of asparaginase on proliferation, cell cycle and apoptosis of diffuse large B-cell lymphoma (DLBCL) cell lines.@*METHODS@#CCK-8 assay was used to detect the effect of asparaginase on proliferation of DLBCL cell lines. Flow cytometry was used to analyze cell cycle and apoptosis. Western blot was used to analyze apoptosis and its potential mechanism.@*RESULTS@#Asparaginase obviously inhibited the proliferation of multiple DLBCL cell lines and caused G/G cell arrest. Furtherly, asparaginase inhibited the expression of HIF-1α which related to poor prognosis of patients with DLBCL, up-regulated the expression of DR4 and caspase 8, reduce the expression of c-FLIP. Meanwhile, asparaginase induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1.@*CONCLUSION@#Asparaginase can inhibit the proliferation of DLBCL cell lines, cause the arrest of cells in G/G and induce apoptosis via the endogenous and exogenous apoptotic pathways.


Subject(s)
Apoptosis , Asparaginase , Cell Line, Tumor , Cell Proliferation , Humans , Lymphoma, Large B-Cell, Diffuse
10.
Article in Chinese | WPRIM | ID: wpr-798491

ABSTRACT

Objective:To campare the hepatotoxicity on BRL and nephrotoxicity on NRK caused by dichloromethane site of Genkwa Flos before and after being processed with vinegar. Method:BRL of normal hepatocytes and NRK of normal renal cells in rats were selected as the subjects.Thiazolyl blue tetrazolium bromide method(MTT) was adopted to evaluate the effect of dichloromethane sites of raw and vinegar-processed products on cell activity of NRK and BRL.The levels or contents of aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP),glutathione(GSH),lactate dehydrogenase(LDH),blood urea nitrogen(BUN) were determined in cell culture supernatant and splitting supernatant for evaluation of their oxidative damage effect. Result:Compared with the blank group,dichloromethane site of raw products could obviously inhibit the cell activity of NRK and BRL,and increase the levels of AST,ALT,ALP and LDH(PPPPConclusion:Processing with vinegar can attenuate the hepatotoxicity and nephrotoxicity on NRK and BRL caused by dichloromethane site of Genkwa Flos,it can improve hepatic and renal function and antioxidant capacity.

11.
Article in Chinese | WPRIM | ID: wpr-850799

ABSTRACT

Objective: By screening the quality markers of Ca2+ antagonistic ingredients, a rapid evaluation system about the vasodilatory effect of Angelica sinensis (AS) by near infrared spectroscopy (NIR) was established. Methods: The Ca2+ antagonists in AS were screened by UPLC/Q-TOF combined with Ca2+ double luciferase reporter gene system. To establish the quality markers, the antagonistic effects were further evaluated in cells and in vitro. The quality markers in multi batches of AS were quantitatively analyzed, and the corresponding NIR was obtained, and then the NIR fitting algorithm was established. Meanwhile, the relationship between Ca2+ antagonistic holistic activity of AS extract and the content of quality markers was investigated, and the prediction model for vasodilation efficacy of AS was constructed based on quality markers check analysis via NIR technique. Results: The screening result showed that ligustilide (X1) and levistilide A (X2) in AS had significant Ca2+ antagonistic effects, and the change of the content was consistent with the capacity of Ca2+ antagonistic action of AS extracts, so they were confirmed as quality markers. According to nonlinear regression analysis, the relationship between Ca2+ antagonistic effect (Y) of AS and the content of two quality markers satisfied the following functions: Y = 31.257 9 X1 + 381.352 0 X2 - 248.979 0 X1X2 + 18.482 2. In addition, the measured values from NIR simulation method for ligustilide and levistilide A detection showed a good correlation with the predicted values. Conclusion: In this study, it was found that ligustilide and levistilide A were quality markers responsible for vasodilation efficacy in AS, and the quantitative correlation between quality markers and the function of vasodilation effect was established. With the help of NIR technology, a novel solution for the rapid monitoring of the quality of traditional Chinese medicine was demonstrated.

12.
China Pharmacy ; (12): 3057-3061, 2019.
Article in Chinese | WPRIM | ID: wpr-817441

ABSTRACT

OBJECTIVE: To study the vasodilatory effect of oxysophocarpine (OSC) on isolated thoracic aortic rings of rats and its possible mechanism. METHODS: Thoracic aortic rings of rats were collected (called “vascular ring” for short). Using K-H nutrient solution as blank control and the diastolic rate as index, the effects of different concentrations (0.2-1.0 mg/mL) of OSC on normal vascular rings in basal state, normal or endothelium-free vascular rings pre-contracted by norepinephrine (PE, 1×10-6 mol/L) were investigated. After pre-culturing normal thoracic aortic rings by nitric oxide synthase inhibitor L-nitro-arginine methyl ester(L-NAME)and cyclooxygenase inhibitor indomethacin(INDO),as well as pre-culturing endothelium-free vascular rings by potassium ion channel blocker BaCl2,tetraethylammonium(TEA)and 4-aminopyridine(4-AP), the diastolic effects of OSC of different concentrations (0.2-1.0 mg/mL) on the above vascular rings were investigated by using the same method. RESULTS: Compared with blank control, there was no significant effects of different concentrations of OSC on the diastolic rate of normal vascular rings in basal state (P>0.05), but 0.4-1.0 mg/mL OSC could significantly improve the diastolic rate of normal or endothelium-free vascular rings pre-contracted by PE (P<0.01), in concentration-dependent manner. After preculturing with L-NAME, INDO, 4-AP and BaCl2, different concentrations of OSC had no significant effect on the diastolic rate of normal or endothelium-free vascular rings pre-contracted by PE (P>0.05). After pre-culturing with TEA and Gli, 0.4-1.0 mg/mL OSC could significantly reduce the diastolic rate of endothelium-free vas- cular rings pre-contracted by PE (P<0.01). CONCLUSIONS: OSC did not significantly dilate the thoracic aortic rings of rats in the basal state within the dose range (0.2-1.0 mg/mL), but OSC of 0.4-1.0 mg/mL have significant diastolic effects on the normal or endothelium-free thoracic aortic rings of rats pre-contracted with PE. The mechanism of thoracic aortic rings dilation is endothelium-independent, which may be associated with receptor operational calcium channel,Ca2+-activated potassium channels and ATP-sensitive potassium channels.

13.
Article in Chinese | WPRIM | ID: wpr-734321

ABSTRACT

Objective To investigate the expression of miR-124 in glioblastoma (GBM) cell lines LN229 and LN229R,as well as the regulatory mechanism of miR-124 on radiosensitivity of LN229R cells.Methods miR-124 mimic (miR-124) and negative control (miR-NC),STAT3 overexpression plasmid (STAT3) and pcDNA3.1 vector (pcDNA) were transfected or co-transfected into radioresistant glioma cells LN229R.qRT-PCR was employed to analyze the expression of miR-124 in LN229 and LN229R cells.The survival rate and sensitivity-related parameters of LN229R cells at different doses were analyzed by cloning formation assay.Cell apoptosis of LN229R was evaluated by flow cytometry.Targeting gene of miR-124 was predicted using Targetscan software and verified by the double-luciferase reporter assay.Western blot assay was performed to detect STAT3 protein expression.Results The expression of miR-124 in LN229R cells (0.32 ± 0.03) was significantly lower than that in LN229 cells (1.02 ± 0.09) (t =12.780,P<0.05).Transfection of miR-124 mimics promoted the expression of miR-124 in LN229R cells (4.02±0.39) compared with miR-NC group (0.95±0.06) (t=13.476,P<0.05).After 8 Gy irradiation,the survival rate of LN229R cells transfected with miR-124 mimics (0.003 ± 0.000 4) was significantly lower than that in miR-NC group (0.033±0.005 0) (t=5.655,P<0.05),and the apoptosis rate (22.34±2.42) % was significantly higher than that in miR-NC group (4.69 ± 0.51) % (t =12.361,P<0.05).STAT3 was identified to be a target gene of miR-124.Exogenous restoration of STAT3 reversed the inhibitory effect of miR-124 on LN229R cell survival.Conclusion miR-124 increases the radiosensitivity of LN229R cells by targeting STAT3.

14.
Article in Chinese | WPRIM | ID: wpr-755173

ABSTRACT

Objective To study the clinical value of enhanced recovery after surgery (ERAS) in the perioperative treatment of type Ⅰ (Todami,1975) biliary dilatation (BD) of children.Methods To retrospectively analyze the data of children with type Ⅰ BD who were treated in the General Surgery Department of Zhengzhou Children's Hospital from June 2014 to May 2018.A total of twenty children with type Ⅰ BD treated with ERAS and 20 children treated with the traditional method in our department were selected in this study using the random number table method.Postoperative indicators (including operation time,first defecation time,changes in amylase in blood and abdominal cavity exudates,length of hospital stay,and hospitalization fee) and relevant postoperative complications (including sore throat,nausea and vomiting,urethral pain,upper respiratory tract infection,incision wound infection,adhesive intestinal obstruction,anastomotic leakage and pancreatic fistula) of the ERAS group and the control group were compared.Results The first defecation time,length of hospital stay and hospitalization fee were significantly lower in the ERAS group than the control group (all P < 0.05) [first defecation time (1.98 ± 0.25) d vs.(2.25 ± 0.31) d;length of hospital stay (6.91 ± 1.25) d vs.(9.95 ± 1.53) d;hospitalization fee (23.32 ± 2.25)thousand yuan vs.(25.99 ±3.10) thousand yuan].Moreover,the incidences of sore throat,nausea and vomiting,urethral pain and upper respiratory tract infection were significantly lower in the ERAS group than the control group (all P < 0.05) [the incidences of sore throat (5.0% vs.45.0%);the incidences of sickness and vomiting (5.0% vs.30.0%);the incidences of urethral pain (5.0% vs.45.0%);the incidences of upper respiratory tract infection (5.0% vs.40.0%)].On the other hand,there were no significant differences in the mean operation times,changes in amylase levels in the blood or abdominal cavity exudates,incision wound infection,and incidences of adhesive intestinal obstruction,anastomotic leakage and pancreatic fistula (all P > 0.05).Conclusions ERAS for type Ⅰ BD surgery was safe and reliable in children.It effectively promoted recovery of postoperative gastrointestinal function and reduced the incidence of complications.

15.
Article in English | WPRIM | ID: wpr-812350

ABSTRACT

A series of new hybrids of dehydroandrographolide (TAD), a biologically active natural product, bearing nitric oxide (NO)-releasing moieties were synthesized and designated as NO-donor dehydroandrographolide. The biological activities of target compounds were studied in human erythroleukemia K562 cells and breast cancer MCF-7 cells. Biological evaluation indicated that the most active compound I-5 produced high levels of NO and inhibited the proliferation of K562 (IC 1.55 μmol·L) and MCF-7 (IC 2.91 μmol·L) cells, which were more potent than the lead compound TAD and attenuated by an NO scavenger. In conclusion, I-5 is a novel hybrid with potent antitumor activity and may become a promising candidate for future intensive study.


Subject(s)
Antineoplastic Agents , Chemistry , Cell Proliferation , Diterpenes , Chemistry , Pharmacology , Drug Design , Drug Screening Assays, Antitumor , Humans , K562 Cells , MCF-7 Cells , Nitric Oxide , Chemistry , Pharmacology , Structure-Activity Relationship
16.
Article in English | WPRIM | ID: wpr-773561

ABSTRACT

A series of new hybrids of dehydroandrographolide (TAD), a biologically active natural product, bearing nitric oxide (NO)-releasing moieties were synthesized and designated as NO-donor dehydroandrographolide. The biological activities of target compounds were studied in human erythroleukemia K562 cells and breast cancer MCF-7 cells. Biological evaluation indicated that the most active compound I-5 produced high levels of NO and inhibited the proliferation of K562 (IC 1.55 μmol·L) and MCF-7 (IC 2.91 μmol·L) cells, which were more potent than the lead compound TAD and attenuated by an NO scavenger. In conclusion, I-5 is a novel hybrid with potent antitumor activity and may become a promising candidate for future intensive study.


Subject(s)
Antineoplastic Agents , Chemistry , Cell Proliferation , Diterpenes , Chemistry , Pharmacology , Drug Design , Drug Screening Assays, Antitumor , Humans , K562 Cells , MCF-7 Cells , Nitric Oxide , Chemistry , Pharmacology , Structure-Activity Relationship
17.
Article in Chinese | WPRIM | ID: wpr-806446

ABSTRACT

Objective@#To analyses the antimicrobial resistance and molecular characterization of 21 MRSA isolates cultured from retail foods from different provinces in China, and evaluate the molecular typing methods.@*Methods@#Twenty-one MRSA isolates were obtained from national foodborne pathogen surveillance network in 2012 (Chinese salad, n=3; milk, n=1; cake, n=2; rice, n=1; cold noodle, n=1; spiced beef, n=1; dumpling, n=1; packed meal, n=1; salad, n=1; raw pork, n=9). The antimicrobial resistance of 21 strains to 12 antimicrobial agents was tested by broth dilution method. Polymerase chain reaction (PCR) and DNA sequencing were performed to obtain the genetic types of MLST (ST) and spa typing. The clonal complex (CC) was assigned by eBURST soft and the MLVA type (MT) and MLVA complex (MC) were identified via the database of the MLVA website (http://www.mlva.net). SmaI pulsed-field gel electrophoresis (SmaⅠ-PFGE) was also carried out to obtain the PFGE patterns of 21 strains. The genetic diversity and discriminatory power of typing were calculated by the Simpson's index of diversity (diversity index, DI) to find out the best genotyping method for MRSA.@*Results@#All MRSA isolates showed multi-drug resistance(MDR), and were resistant to oxacillin, benzylpenicillin, clindamycin and erythromycin, and 71.4% (15/21), 47.6% (10/21), 42.9% (9/21) and 28.6% (6/21) of the MRSA isolates were resistant to tetracycline, ciprofloxacin, trimethoprim/sulfamethoxazole and gentamicin, respectively. Moreover, one strain was found to be resistant to all three antimicrobials of levofloxacin, moxifloxacin and rifampicin. Great diversity was found in these food-associated MRSA (6 STs, 7 spa types, and 9 MTs). PFGE patterns were more diverse than those of other three molecular typing methods (19 pulse types). The index of diversity (DI) of PFGE, MLVA, spa typing and MLST was 0.99, 0.80, 0.73, and 0.61, respectively. Among the MRSA isolates, CC9-ST9-t899-MT929-MC2236 (PFGE Cluster Ⅴ) was the most prevalent clone, which were all cultured from raw pork (9 isolates). Besides, two MRSA were identified as CC59-ST338-t437-MT621-MC621 (PFGE Cluster Ⅳ). Different clone had their own resistance spectrum profiles.@*Conclusion@#The food-borne MRSA isolates were all MDR in this study. Different clones had their own resistance spectrum profiles. MLVA represented a promising tool for molecular epidemiology tracing of MRSA in foodborne disease events.

18.
Journal of Medical Postgraduates ; (12): 1172-1176, 2018.
Article in Chinese | WPRIM | ID: wpr-818004

ABSTRACT

ObjectiveChronic pancreatitis (CP) is an irreversible pancreatic parenchymal disease with complicated etiology. Chymotrypsin C (CTRC) gene variation may be related to CP occurrence. The article analyzed the mutation of CTRC gene in Han population with CP in Sichuan, and discussed its clinical relevance.MethodsPeripheral blood samples were collected from 106 patients with CP and 148 healthy controls and DNA was extracted for whole exon sequencing of CTRC gene and analysis of clinical correlation.ResultsOne case of c.611G>A heterozygous missense mutation and three cases of single nucleotide polymorphism (SNP) site variation were found which included one new SNP c.40+133G>A and two known SNPs: rs6679763 and rs555015. The variation of c.40 +133G> A and rs6679763 showed a significant difference between case group and control group (P=0.029, P=0.011). Clinical baseline data showed significant differences between two groups on smoking (P=0.042), biliary disease (P=0.013), and blood glucose(P=0.017). When the confounding factors were eliminated, we found that smoking and rs6679763 variant site were significantly associated with CP risk (OR=2.817, 95%CI: 1.016-7.811, P=0.047;OR = 4.893, 95%CI: 1.152-20.781, P=0.031, respectively). There was no multiplicative interaction between gene mutation and environment or clinical data.ConclusionSmoking, biliary disease, blood glucose, c.611G>A mutation, rs6679763 and c.40+133G>A variation may be related to the occurrence of CP. Smoking and rs6679763 locus variation are independent risk factors for CP, and the CTRC gene SNP locus rs6679763 and c.40+133G>A and the point mutation site c.611G>A are predisposing loci in Chinese Han population of Sichuan province.

19.
Article in Chinese | WPRIM | ID: wpr-690498

ABSTRACT

To screen the toxic polar fractions of Daphne genkwa, compare the toxicity of D. genkwa on crypts epithelial cells IEC-6 before and after vinegar processing, and preliminarily investigate the mechanism of D. genkwa vinegar processing on toxicity reducing. The proliferation of IEC-6 cells was observed by MTT. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), lactate dehydrogenase (LDH), as well as the enzyme activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase were determined in IEC-6 cells to evaluate the oxidative damages degree of IEC-6 cells. The apoptosis and cell cycle were analyzed by Flow Cytometry. The results showed that the dichloromethane extraction was the toxic polar fraction of D. genkwa, and after vinegar processing, the toxicity of dichloromethane fraction was significantly reduced (<0.01). As compared with the blank control group, the dichloromethane fraction of D. genkwa can obviously decrease the levels of SOD, Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-ATPase (<0.01) and content of GSH, but increase the level of LDH and MDA in cell supernatant (<0.01). Besides, it obviously increased the early and late apoptotic rate of IEC-6 cells, obviously decreased the proportion of G₁stage cells, increased the ratio of S stage cells and M stage cells (<0.01). After vinegar processing, as compared with D. genkwa groups of various doses, it can significantly increase the levels of SOD, Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-ATPase (<0.01) and content of GSH, decrease the level of LDH, MDA(<0.01), significantly decrease the early and late apoptosis rate of IEC-6 cells (<0.01), increase the proportion of G₁stage cells, and decrease the ratio of S stage cells and M stage cells (<0.01). Vinegar processing can reduce the toxicity of dichloromethane fraction of D. genkwa, and its mechanism may be associated with improving the activity of antioxidant enzymes and permeability in IEC-6 cells, and decreasing the oxidative damage.

20.
Article in Chinese | WPRIM | ID: wpr-690454

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of chloroquine in inducing apoptosis of human hepatocellular carcinoma cells and explore the possible mechanism.</p><p><b>METHODS</b>MTT assay and flow cytometry were used to evaluate chloroquine-induced growth inhibition and apoptosis in human hepatocellular carcinoma HepG2 cells, respectively. The ATP levels in chloroquine-treated cells were detected using an ATP assay kit. PCR and Western blotting were used to detect the expression levels of miR-26b and Mcl-1 in the cells, respectively.</p><p><b>RESULTS</b>Chloroquine inhibited the proliferation of HepG2 cells in a time- and concentration-dependent manner. Treatments with 80 µmol/L chloroquine for 24, 48, and 72 h induced survival rates of (71.59∓0.2)%, (45.40∓0.5)%, and (26.34∓1.4)% in the cells. Treatments with chloroquine at 40, 80, and 160 µmol/L for 5 h resulted in obviously lowered intracellular ATP levels in the cells to 87.80%, 71.29%, and 38.02% of the control level, respectively. At 80 µmol/L, chloroquine significantly increased the expression of miR-26b and down-regulated the expression of Mcl-1 in HepG2 cells, and the application of the miR-26b inhibitor increased the cellular expression of Mcl-1.</p><p><b>CONCLUSION</b>s Chloroquine can inhibit the cell proliferation, reduce ATP level and induce apoptosis in HepG2 cells possibly through miR-26b-mediated regulation of Mcl-1.</p>

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