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1.
Chinese Journal of School Health ; (12): 1907-1911, 2023.
Article in Chinese | WPRIM | ID: wpr-1004917

ABSTRACT

Abstract@#Children and adolescents are at an important stage in the development of eating habits and food skills. Nutrition literacy is inseparable from healthy eating behaviors and higher nutritional quality, and improving nutrition literacy is an important pathway to better nutrition. The paper summarizes domestic and international research on nutrition literacy from the perspectives of definition and assessment tools in children and adolescents. It is found that there is no universal definition of nutritional literacy. Adult nutrition literacy or with appropriate adjustments are widely used in children and adolescents, without taking into account the unique developmental characteristics and nutritional needs of children and adolescents; the measurement tools of nutritional literacy are diverse, and there is no unified assessment tools, so it is difficult to compare and analyze research findings and draw reliable conclusions. In the future, authoritative definitions, and standardized evaluation tools are in great need.

2.
Acta Pharmaceutica Sinica ; (12): 1913-1921, 2023.
Article in Chinese | WPRIM | ID: wpr-978665

ABSTRACT

One of the traditional prescriptions for treating lung diseases, Jiegeng decoction (JGT), is still unknown in terms of its chemical makeup and mechanism. In this study, Q-Exactive-Orbitrap MS technology was used to identify the chemical constituents of JGT, and metabolomics was used to examine the effect of JGT on metabolites in the lung tissue of mice with acute lung injury (ALI) model. The potential biomarkers were screened by fold change (FC) > 1.5 or FC < 0.67 and P < 0.05, and enriched for metabolic pathways. A total of 40 compounds, including triterpenoid saponins, flavonoids and glycosides, were identified by mass spectrometry analysis of JGT. All animal experiments were approved by the Experimental Animal Ethics Committee of Tianjin University of Traditional Chinese Medicine (No. TCM-LAEC2021106). The results showed that JGT improved the lung coefficient, and lung tissue morphology of mice with ALI, lowered the levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) in bronchoalveolar lavage fluid (BALF), and reduced myeloperoxidase (MPO) content in lung tissue. The metabolomic results showed that JGT could regulate 22 metabolites associated with ALI, among which leukotriene D4, docosapentaenoic acid, hypoxanthine, L-5-oxoproline, and other metabolites were mainly associated with the body′s inflammatory response and oxidative stress, and were enriched in the pathways of glutathione metabolism, purine metabolism, and primary bile acid biosynthesis. This study analyzed the potential mechanism of JGT in the treatment of ALI through metabolomics, providing an important theoretical basis for the clinical application of JGT.

3.
Chinese Journal of Biotechnology ; (12): 881-897, 2023.
Article in Chinese | WPRIM | ID: wpr-970411

ABSTRACT

Facing the increasingly severe energy shortage and environmental pollution, electrocatalytic processes using electroactive microorganisms provide a new alternative for achieving environmental-friendly production. Because of its unique respiratory mode and electron transfer ability, Shewanella oneidensis MR-1 has been widely used in the fields of microbial fuel cell, bioelectrosynthesis of value-added chemicals, metal waste treatment and environmental remediation system. The electrochemically active biofilm of S. oneidensis MR-1 is an excellent carrier for transferring the electrons of the electroactive microorganisms. The formation of electrochemically active biofilm is a dynamic and complex process, which is affected by many factors, such as electrode materials, culture conditions, strains and their metabolism. The electrochemically active biofilm plays a very important role in enhancing bacterial environmental stress resistance, improving nutrient uptake and electron transfer efficiency. This paper reviewed the formation process, influencing factors and applications of S. oneidensis MR-1 biofilm in bio-energy, bioremediation and biosensing, with the aim to facilitate and expand its further application.


Subject(s)
Bioelectric Energy Sources/microbiology , Biofilms , Electrodes , Electron Transport , Shewanella/metabolism
4.
China Journal of Chinese Materia Medica ; (24): 6396-6402, 2023.
Article in Chinese | WPRIM | ID: wpr-1008838

ABSTRACT

A quantitative proton nuclear magnetic resonance(qHNMR) method was established to determine the glucose content in commercially available Massa Medicata Fermentata(MMF) products and explore the variations of glucose content in MMF products during processing. The qHNMR spectrum of MMF in deuterium oxide was obtained with 2,2,3,3-d_4-3-(trimethylsilyl) propionate sodium salt as the internal standard substance. With the doublet peaks of terminal hydrogen of glucose with chemical shift at δ 4.65 and δ 5.24 as quantitative peaks, the content of glucose in MMF samples was determined. The glucose content showed a good linear relationship within the range of 0.10-6.44 mg·mL~(-1). The relative standard deviations(RSDs) of precision, stability, repeatability, and recovery for determination were all less than 2.3%. The glucose content varied in different commercially available MMF samples, which were associated with the different fermentation days, wheat bran-to-flour ratios, and processing methods. The glucose content in MMF first increased and then decreased over the fermentation time. Compared with the MMF products fermented with wheat bran or flour alone, the products fermented with both wheat bran and flour had increased glucose. The glucose content of bran-fried MMF was slightly lower than that of raw MMF, while the glucose content in charred MMF was extremely low. In conclusion, the qHNMR method established in this study is simple, fast, and accurate, serving as a new method for determining the glucose content in MMF. Furthermore, this study clarifies the variations of glucose content in MMF during processing, which can not only indicate the processing degree but also provide a scientific basis for revealing the fermentation mechanism and improving the quality control of MMF.


Subject(s)
Protons , Drugs, Chinese Herbal/chemistry , Dietary Fiber , Magnetic Resonance Spectroscopy
5.
Chinese Journal of Clinical Nutrition ; (6): 95-99, 2023.
Article in Chinese | WPRIM | ID: wpr-991914

ABSTRACT

Objective:To investigate the serum 25-hydroxyvitamin D [25(OH)D] level in patients with Graves' disease (GD) and its correlation with thyrotropin receptor antibody (TRAb) and bone metabolism markers.Methods:A total of 124 patients with newly diagnosed or relapsed GD were selected and divided into three groups according to serum 25(OH)D level, namely vitamin D deficiency group with 25(OH)D <12 μg/L, vitamin D insufficiency group with 25(OH)D of 12 to 20 μg/L, and vitamin D sufficiency group with 25(OH)D ≥ 20 μg/L. The levels of serum 25(OH)D, TRAb, type I procollagen N-terminal pro-peptide (PINP), type I collagen cross-linked C-terminal peptide (S-CTX), parathyroid hormone (PTH), total triiodothyronine (TT 3), total thyroxine (TT 4) and thyroid-stimulating hormone (TSH) were measured in all patients, and the differences of these biochemical indices were compared across groups. Oneway analysis of variance or Kruskal-Wallis test was used for comparison between groups, and Pearson or Spearman correlation analysis was applied for correlation test. Results:The levels of serum TT 3, TT 4, PINP, and S-CTX significantly increased ( P < 0.01) and the level of phosphorus (P) decreased ( P < 0.01) with the decreased vitamin D levels. The levels of PTH and calcium (Ca) were significantly lower in the vitamin D sufficiency group compared with the vitamin D insufficiency group and vitamin D deficiency group ( P < 0.01). Correlation analysis showed that serum 25(OH)D level was negatively correlated with the levels of TT 3, TT 4, PINP, S-CTX, PTH and Ca ( P < 0.01), and positively correlated with the levels of P and TSH ( P < 0.01). Conclusions:Decreased serum 25(OH)D level is closely related with increased bone turnover, PTH, and thyroid hormone levels in patients with GD, but not related with TRAb. Thyroid hormone levels have a certain predictive value regarding vitamin D deficiency in GD patients. It is necessary to monitor the vitamin D levels in patients with GD and provide vitamin D supplementation to reduce the incidence of osteoporosis, improve the effectiveness of antithyroid treatment and reduce the recurrence of GD.

6.
International Journal of Traditional Chinese Medicine ; (6): 464-471, 2023.
Article in Chinese | WPRIM | ID: wpr-989656

ABSTRACT

Objective:To analyze the mechanism of Jindan Tablets, Xiaoyan Lidan Tablets and ursodeoxycholic acid in the treatment of gallstone and cholecystitis based on network pharmacology; To conduct a comparative analysis.Methods:The chemical components of Jindan Tablets, Xiaoyan Lidan Tablets and ursodeoxycholic acid and their drug targets were collected from Traditional Chinese Medicine Database and Analysis Platform (TCMSP). DAVID 6.8 database was used to search for the associated diseases of the drug targets. The disease targets of gallstone and cholecystitis were collected from GeneCards and other databases. The protein-protein interactions network was established based on the intersecting targets of three drugs and two diseases. KEGG enrichment analysis was performed based on the DAVID 6.8 database. Cytoscape 3.7.1 software was used to construct a complex network and topology analysis of component- target- disease between three drugs and diseases.Results:222 chemical components and 3 133 drug targets were collected for Jindan Tablets. 104 chemical components and 1 425 action targets were collected for Xiaoyan Lidan Tablets. 1 chemical component and 119 action targets were collected for ursodeoxycholic acid. The three drugs were associated with 31 diseases. 1 382 disease targets for gallstones and cholecystitis were collected. There were 237, 163 and 33 targets for gallstones and cholecystitis in the three drugs, of which 17 were shared by the three drugs and 20 were shared by Jindan Tablets and Xiaoyan Lidan Tablets. Based on the DAVID database, 113, 74 and 10 significant KEGG enrichment pathways were obtained for the three drugs respectively.Conclusions:The three drugs shared many targets and pathways in the treatment of gallstones and cholecystitis, which all had the function of regulating metabolism and inhibiting inflammatory response, while participating in apoptosis, oxidative stress and cancer pathology process. However, they had their own special effects, with Jindan Tablets favoring involving in the cancer process and inhibition of inflammation, and promoting angiogenesis. Xiaoyan Lidan Tablets and ursodeoxycholic acid focused on regulating cholesterol metabolism, and Xiaoyan Lidan Tablets also regulated steroid metabolism and inhibit inflammation, while ursodeoxycholic acid regulated bile acid metabolism.

7.
International Journal of Traditional Chinese Medicine ; (6): 1327-1346, 2022.
Article in Chinese | WPRIM | ID: wpr-954468

ABSTRACT

The standardization of pediatric Tuina is beneficial to pediatric Tuina practitioners in a norm practices. The paper collects the content from teaching textbooks, TCM ancient books and database literature, and tries to develop the technical specifications of pediatric Tuina by four rounds Delphi surveys and expert consensus. This specification covers the manipulation of pediatric Tuina, the position of acupoints, the effects of acupoints and the diagnosis and treatment of pediatric Tuina, including indications, contraindications, cautious use, operation steps and methods.

8.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 19-25, 2022.
Article in Chinese | WPRIM | ID: wpr-940513

ABSTRACT

ObjectiveTo explore the pharmacodynamic effect of gramine on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice and its potential mechanism. MethodThe mice were divided into the normal control group, model group, dexamethasone (0.05 g·kg-1) group, and high- and low-dose (0.12,0.06 g·kg-1) gramine groups. Mice in all groups except for the normal control group were stimulated with DNCB, followed by medication 13 d later. The changes in skin lesions were then observed, and the skin thickness, moisture content, and transepidermal water loss (TWEL) in each group were measured. The pathological changes in skin lesions were observed by hematoxylin-eosin (HE) staining, and the effects of drugs on CD4+/CD8+T-cell ratio in the spleen were detected by flow cytometry. The levels of immunoglobulin E (IgE), interleukin (IL)-4, and IL-6 in serum were detected by enzyme-linked immunosorbent assay (ELISA), and the changes in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (CRE) by microplate method. The mRNA expression levels of inflammatory cytokines γ-interferon(IFN-γ), IL-13, IL-17, IL-1β, IL-6, and tumor necrosis factor-α (TNF-α) in skin lesions were assayed by real-time polymerase chain reaction (Real-time PCR), and the protein expression levels of nuclear transcription factor -κB (NF-κB) and NF-κB inhibitory protein α (IκBα) in skin lesions by Western blot. ResultCompared with the normal control group, the model group showed skin edema, erythema, scab, scratch, and lymphocyte and neutrophil infiltration, decreased skin moisture content, as well as increased skin thickness, TWEL (P<0.01), spleen index, CD4+/CD8+ T-cell ratio in the spleen (P<0.05), mRNA expression of IFN-γ, IL-13, IL-17, IL-1β, IL-6, and TNF-α in the skin lesions (P<0.05), serum contents of IgE, IL-4, and IL-6 (P<0.05), and protein expression of IκBα and NF-κB in skin lesions (P<0.05). Compared with the model group, dexamethasone and gramine at different doses alleviated skin erythema, scale, scab, and inflammatory cell infiltration, elevated skin moisture content, inhibited skin thickening and TWEL, and decreased spleen index, CD4+/CD8+T-cell ratio in the spleen, mRNA expression of inflammatory factors in the skin lesions, serum contents of IgE and inflammatory factors, and protein expression of IκBα and NF-κB in skin lesions, especially in the dexamethasone group and the high- dose gramine group(P<0.05,P<0.01). ConclusionGramine can inhibit the expression of related inflammatory factors and regulate the immune function of AD mice via the IκBα/NF-κB pathway, enabling it become a potential drug for treating AD.

9.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 204-213, 2022.
Article in Chinese | WPRIM | ID: wpr-940404

ABSTRACT

This scoping review aimed to summarize the application information and clinical studies of oral Chinese patent medicines. The oral Chinese patent medicines in treating lung cancer were screened out by searching pf the drug directory, related guidelines, and medical information websites. The data including functions, application, ingredients, and prices of these medicines were collected. Six public databases were searched with the time interval of establishment to August 22, 2021 for collection of the clinical studies of oral Chinese patent medicines in the treatment of lung cancer. The expert consensuses, systematic reviews, randomized controlled trials, non-randomized controlled trials, and non-controlled trials were selected for analysis. A total of 104 oral Chinese patent medicines were screened out, including 31 capsules, 16 granules, 20 oral liquids, 17 tablets, 17 pills, and 3 ointments, in which altogether 198 herbal medicines were involved. The single-dose prices of 2, 36, and 66 medicines were > CNY 100, CNY 10-100, and < CNY 10, respectively. There were 410 clinical studies associated with 48 oral Chinese patent medicines, which were published from 1986 to 2021. These publications included 1 expert consensus, 21 systematic reviews, 277 randomized controlled trials, 87 non-randomized controlled trials, and 24 non-controlled trials. In the clinical studies, the Chinese patent medicines were usually applied in combination with radiotherapy and chemotherapy. The evaluation of primary outcomes focused on 9 indicators including clinical efficacy, quality of life, and incidence of side effects. In conclusion, the oral Chinese patent medicines demonstrated significant advantages in the treatment of lung cancer, and the relevant clinical trials were increasing year by year, with multiple outcome indicators being evaluated. More comprehensive and standardized clinical studies need to be designed for oral Chinese patent medicines in treating lung cancer in the future.

10.
Journal of Experimental Hematology ; (6): 559-564, 2022.
Article in Chinese | WPRIM | ID: wpr-928754

ABSTRACT

OBJECTIVE@#To construct a mouse model of Glanzmann's thrombasthenia (GT) with ITGA2B c.2659 C>T (p.Q887X) nonsense mutation by CRISPR/Cas9 technology, and then further explore the expression and function of glycoprotein αIIbβ3 on the surface of platelet membrane.@*METHODS@#The donor oligonucleotide and gRNA vector were designed and synthesized according to the ITGA2B gene sequence. The gRNA and Cas9 mRNA were injected into fertilized eggs with donor oligonucleotide and then sent back to the oviduct of surrogate mouse. Positive F0 mice were confirmed by PCR genotyping and sequence analysis after birth. The F1 generation of heterozygous GT mice were obtained by PCR and sequencing from F0 bred with WT mice, and then homozygous GT mice and WT mice were obtained by mating with each other. The phenotype of the model was then further verified by detecting tail hemorrhage time, saphenous vein bleeding time, platelet aggregation, expression and function of αIIbβ3 on the surface of platelet.@*RESULTS@#The bleeding time of GT mice was significantly longer than that of WT mice (P<0.01). Induced by collagen, thrombin, and adenosine diphosphate (ADP), platelet aggregation in GT mice was significantly inhibited (P<0.01, P<0.01, P<0.05). Flow cytometry analysis showed that the expression of αIIbβ3 on the platelet surface of GT mice decreased significantly compared with WT mice (P<0.01), and binding amounts of activated platelets to fibrinogen were significantly reduced after thrombin stimulation (P<0.01). The spreading area of platelet on fibrinogen in GT mice was significantly smaller than that in WT mice (P<0.05).@*CONCLUSION@#A GT mouse model with ITGA2B c.2659 C>T (p.Q887X) nonsense mutation has been established successfully by CRISPR/Cas9 technology. The aggregation function of platelet in this model is defective, which is consistent with GT performance.


Subject(s)
Animals , Humans , Mice , CRISPR-Cas Systems , Codon, Nonsense , Disease Models, Animal , Fibrinogen/genetics , Integrin alpha2/genetics , Oligonucleotides , Platelet Glycoprotein GPIIb-IIIa Complex/genetics , Thrombasthenia/genetics , Thrombin/genetics
11.
Journal of Experimental Hematology ; (6): 323-326, 2022.
Article in Chinese | WPRIM | ID: wpr-928714

ABSTRACT

Tubulin affects platelets count through the control of mitosis and the formation of pro-platelets during the maturation of megakaryoblast to platelets. Tubulin is involved in maintaining the integrity of platelet skeleton, and also participates in the change of platelet morphology during platelet activation. Some new anti-tumor drugs targeting cell mitosis are trying to reduce the effect on tubulin in order to reduce the side effect of drugs on platelet formation. In some patients with thrombocytopenia, the variation and polymorphism of the tubulin gene affect the structure of microtubule multimers, which leads to the decrease of platelet formation. This review summarized the latest progresses of tubulin in the regulation of megakaryopoiesis and thrombopoiesis.


Subject(s)
Humans , Blood Platelets , Megakaryocytes , Platelet Count , Thrombopoiesis , Tubulin
12.
Tissue Engineering and Regenerative Medicine ; (6): 141-150, 2022.
Article in English | WPRIM | ID: wpr-919379

ABSTRACT

BACKGROUND@#Scleroderma is a multisystem disease in which tissue fibrosis is caused by inflammation and vascular damage. The mortality of scleroderma has remained high due to a lack of effective treatments. However, exosomes derived from human umbilical cord mesenchymal stem cells (HUMSCs)-Ex have been regarded as potential treatments for various autoimmune diseases, and may also act as candidates for treating scleroderma. @*METHODS@#Mice with scleroderma received a single 50 lg HUMSCs-Ex. HUMSCs-Ex was characterized using transmission electron microscopy, nanoparticle tracking analysis and nanoflow cytometry. The therapeutic efficacy was assessed using histopathology, immunohistochemistry, immunofluorescence, quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay and western blot. @*RESULTS@#HUMSCs-Ex ameliorated the deposition of extracellular matrix and suppressed the epithelial-mesenchymal transition process, and the effects lasted at least three weeks. In addition, HUMSCs-Ex promoted M1 macrophage polarization and inhibited M2 macrophage polarization, leading to the restoration of the balance of M1/M2 macrophages. @*CONCLUSION@#We investigated the potential antifibrotic and anti-inflammatory effects of HUMSCs-Ex in a bleomycininduced mouse model of scleroderma. So HUMSCs-Ex could be considered as a candidate therapy for scleroderma.

13.
Journal of Clinical Hepatology ; (12): 1578-1581., 2021.
Article in Chinese | WPRIM | ID: wpr-886123

ABSTRACT

ObjectiveTo investigate the value of platelet-albumin-bilirubin score (PALBI) in predicting the 30-day mortality of patients with liver cirrhosis and acute upper gastrointestinal bleeding (AUGIB). MethodsA retrospective analysis was performed for the clinical data of 211 patients with liver cirrhosis who were admitted to Jinshan Hospital of Fudan University due to AUGIB from January 2016 to February 2020, and according to the survival status within 30 days, they were divided into death group with 24 patients and survival group with 187 patients. Epidemiological data (including age and sex) and laboratory examination results (including routine blood test results, hepatic and renal function, and coagulation function) were collected, and the scores of PALBI, albumin-bilirubin (ALBI), Child-Turcotte-Pugh (CTP), and Model for End-Stage Liver Disease (MELD) on admission were calculated and compared between the two groups. The t-test was used for comparison of continuous data between groups, and the chi-square test was used for comparison of categorical data between groups. With the application of 95% confidence interval, the receiver operating characteristic (ROC) curve and the area under the ROC curve (AUC) were used to evaluate the predictive ability of the model. The DeLong test was used for comparison of ROC curve. ResultsCompared with the survival group, the death group had significantly higher PALBI score (-1.47±0.35 vs -1.94±0.36, P<0.001), ALBI score (-0.74±0.49 vs -1.38±0.51, P<0.001), CTP score (10.25±1.98 vs 8.06±1.70, P<0.001), and MELD score (17.25±4.68 vs 11.63±4.83, P<0.001). PALBI, ALBI, CTP, and MELD scores had an AUC of 0.827, 0.824, 0.790, and 0811, respectively, and there was no significant difference in AUC between any two scores (P>0.05). ConclusionPALBI score has good performance in predicting the 30-day mortality of patients with liver cirrhosis and AUGIB and is comparable to CTP and MELD scores.

14.
Journal of Experimental Hematology ; (6): 1175-1180, 2021.
Article in Chinese | WPRIM | ID: wpr-888535

ABSTRACT

OBJECTIVE@#To investigate the clinical efficacy of high dose methotrexate (HD-MTX), temozolomide (TMZ), and rituximab (R) in the treatment of patients with primary central nervous system lymphoma (PCNSL).@*METHODS@#Clinical data of patients with PCNSL diagnosed and treated in Guangdong Provincial People's Hospital from February 2010 to May 2017 were collected. First, patients were given 6-8 cycles of MTX (3.5 g/m@*RESULTS@#There were 42 patients enrolled in the study, 17 cases in HD-MTX+TMZ group and 25 cases in HD-MTX+TMZ+R group. The median PFS and OS times in HD-MTX+TMZ+R group were 56.7 months and N/A, respectively, while, 7.3 months and 34.7 months in HD-MTX+TMZ group, respectively. In addition, there was no significant difference in median survival between patients who received TMZ maintenance therapy and those who were only actively monitored. During the induction period, all the patients had grade 1-2 nausea and vomiting, while in the consolidation treatment period, no grade 3/4 toxicity was observed.@*CONCLUSION@#The combination of HD-MTX+TMZ+R in the treatment of PCNSL patients shows a definite short-term effect, which can increase the survival rate of the patients. The side effects are mild, and the patients can generally tolerate.


Subject(s)
Humans , Antineoplastic Combined Chemotherapy Protocols , Central Nervous System , Central Nervous System Neoplasms/drug therapy , Lymphoma, Non-Hodgkin/drug therapy , Methotrexate/therapeutic use , Retrospective Studies , Rituximab/therapeutic use , Temozolomide/therapeutic use , Treatment Outcome
15.
Journal of Experimental Hematology ; (6): 591-595, 2021.
Article in Chinese | WPRIM | ID: wpr-880118

ABSTRACT

OBJECTIVE@#To test the anticoagulation functions, perform the genetic diagnosis and analyze the clinical characteristics in a family with combined heterozygous genetic variants of PROC and PROS1.@*METHODS@#Peripheral blood was collected from all the family members. Hematological phenotypes and activity of anticoagulant factors were analyzed. Target genes were amplified by PCR from DNA isolated from peripheral blood, and then were analyzed by Sanger DNA sequencing.@*RESULTS@#Many members in the family displayed the combined genetic variants in protein C and protein S, and six family members accompanied by deep venous thrombosis (DVT). The influences of genetic and secondary factors on the incidence of venous thrombosis in the family members were analyzed. The results showed that in this family, carriers of combined protein C and protein S gene defects had a higher incidence of VTE, but acquired factors still played a key role in the eventual thrombotic symptoms.@*CONCLUSION@#Venous thromboembolism (VTE) is a multifactorial disease, the combined genetic heterozygous mutations of protein C and S is an important genetic factor, and the clinical phenotype show a high heterogenicity, the secondary factors contribute to the VTE incidence.


Subject(s)
Humans , Heterozygote , Mutation , Protein C/genetics , Protein S/genetics , Risk Factors , Venous Thromboembolism , Venous Thrombosis/genetics
16.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 216-224, 2021.
Article in Chinese | WPRIM | ID: wpr-906070

ABSTRACT

Sepsis, a common critical disease in the intensive care unit(ICU), features high morbidity and mortality. At present, it is mainly tackled with western medicine, which may trigger a series of problems like antibiotic resistance, adverse hormonal reactions, and high cost after a long-term use. Therefore, exploring new efficient, safe, and cheap drugs and treatment modes has become the focus of our research at this stage. By virtue of unique advantages including "the concept of holism and individualized treatment based on syndrome differentiation", Chinese medicine has accumulated quite rich experience in the prevention and treatment of sepsis. In recent years, research on the regulation of Chinese medicine on nuclear transcription factor-κB(NF-κB) signaling pathway in sepsis has kept emerging. On this basis, this paper reviewed the etiology and pathogenesis of sepsis, syndrome differentiation and treatment, NF-κB signaling pathway, and its intervention with Chinese medicine. It has been found that some single Chinese herbs and their extracts, Chinese herbal compounds, and Chinese herbal injections effectively inhibit the expression of such inflammatory factors as NF-κB-mediated tumor necrosis factor-α(TNF-α), interleukin-1(IL-1), and IL-6 as well as the related proteins, reduce the systemic inflammatory response and organ injury, and improve the prognosis by regulating the activation of NF-κB signaling pathway and the immune function of macrophages. However, due to the limitations of objective conditions, some studies also have the problems of fuzzy pro-inflammatory anti-inflammatory balance mechanism, unclear pharmacokinetics and low drug safety evaluation, which need to be further studied and explored in order to provide a new theoretical basis and diagnosis and treatment thinking for the treatment of sepsis with traditional Chinese medicine.

17.
Chinese Journal of Medical Genetics ; (6): 106-109, 2020.
Article in Chinese | WPRIM | ID: wpr-781288

ABSTRACT

OBJECTIVE@#To detect genetic variants among 7 pedigrees affected with ornithine transcarbamylase deficiency (OTCD) and provide prenatal diagnosis for them.@*METHODS@#The pedigrees were subjected to targeted next-generation sequencing (NGS) with a gene panel for inherited metabolic diseases. Suspected pathological variants were confirmed by Sanger sequencing of the probands and their family members. Prenatal diagnosis was provided for 4 of the pedigrees.@*RESULTS@#Seven variants of the OTC gene, including c.583G>A (p.Gly195Arg), c.626C>T (p.Ala209Val), c.674C>T(p.Pro225Leu), c.482A>G (p.Asn161Ser), IVS1-2A>G, c.116G>T(p.Gly39Val), c.898delT(p.300Phefs*22), were detected, among which IVS1-2A>G, c.116G>A (p.Gly39Val) and c.898delT (p.300Phefs*22) were unreported previously. Three male fetuses were found to carry hemizygotic variants upon prenatal diagnosis. One female fetus were found to be heterozygous for a variant, and appeared to be normal at birth as well as by newborn screening.@*CONCLUSION@#OTC gene variant is the etiology of OTCD in the 7 pedigrees. Genetic testing of OTC could assist physicians in OTCD diagnosis and provide genetic counseling and prenatal diagnosis for the pedigrees.

18.
Chinese Journal of Medical Genetics ; (6): 151-153, 2019.
Article in Chinese | WPRIM | ID: wpr-775791

ABSTRACT

OBJECTIVE@#To explore the clinical features and genetic diagnosis of two cases with rare diseases and X chromosome abnormalities.@*METHODS@#Multiple ligation-dependent probe amplification (MLPA) and karyotype analysis were carried out on an 8-year-old girl who was diagnosed with Duchenne muscular dystrophy. Karyotype analysis and PCR assay for SRY and AZF genes were carried out for a-2-month-old male infant with short penis.@*RESULTS@#The girl, who featured short stature and cubitus valgus, was diagnosed as Turner syndrome with a karyotype of 46,X,i(Xq). The male infant was detected with a karyotype of 45,X, with presence of SRY gene but absence of AZF gene.@*CONCLUSION@#Both cases may be associated with abnormalities of X chromosome. Genetic testing can facilitate early diagnosis and clinical intervention for such patients.


Subject(s)
Humans , Infant , Male , Chromosomes, Human, X , Karyotyping , Muscular Dystrophy, Duchenne , Genetics , Rare Diseases , Turner Syndrome , Genetics
19.
Chinese Journal of Natural Medicines (English Ed.) ; (6): 729-737, 2019.
Article in English | WPRIM | ID: wpr-776834

ABSTRACT

Non-small cell lung cancer (NSCLC) accounts for 80%-85% of all lung malignancies and good diagnosis and prognosis of NSCLC are critical to the increase of its survival rate. Tumor-associated macrophages (TAM) abundantly present in numerous cancer types, and the role of TAMs in tumor biology and their prognostic value in cancer become major topics of interest. After various stimulations in the tumor microenvironment, TAMs develop into a M1 (tumor-inhibitory) phenotype or M2 (tumor-promoting) phenotype. Recent studies show that traditional Chinese medicine (TCM) monomers have markedly inhibitory actions for NSCLC through M1/M2 modulation. Due to the TCM monomers mainly covered five categories, i.e. terpenoids, flavonoids, polysaccharides, natural polyphenols, and alkaloids. Thus, we will discuss the regulation of TCM monomers on TAM involve in these five parts in this review. In addition, the potential role of TAMs as therapeutic targets will be discussed.

20.
Chinese Journal of Contemporary Pediatrics ; (12): 663-669, 2019.
Article in Chinese | WPRIM | ID: wpr-775127

ABSTRACT

OBJECTIVE@#To investigate the composition of gut microbiota and its correlation with the severity of behavior symptoms in children with autism spectrum disorder (ASD).@*METHODS@#A total of 30 children with ASD were enrolled as the ASD group, and 20 healthy children matched for age and sex were enrolled as the healthy control group. Related clinical data were analyzed. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene in fecal samples were sequenced. The severity of behavior symptoms in children with ASD was assessed using the autism behavior checklist. The Spearman's correlation analysis was used to investigate the correlation between gut microbiota and the severity of behavior symptoms in children with ASD.@*RESULTS@#There was a significant difference in the composition of gut microbiota between the two groups. Compared with the healthy control group, the ASD group had significant reductions in Shannon index and Shannoneven index (P<0.05), as well as a significant reduction in the percentage of Firmicutes and a significant increase in the percentage of Acidobacteria in feces (P<0.05). In the ASD group, the dominant bacteria were Megamonas, Megasphaera, and Barnesiella, while in the healthy control group, the dominant bacteria were Eubacterium_rectale_group, Ezakiella, and Streptococcus. In the children with ASD, the abundance of Megamonas was positively correlated with the scores of health/physical/behavior and language communication (P<0.05).@*CONCLUSIONS@#The development of ASD and the severity of behavior symptoms are closely associated with the composition of gut microbiota.


Subject(s)
Child , Humans , Autism Spectrum Disorder , Bacteria , Feces , Gastrointestinal Microbiome , RNA, Ribosomal, 16S
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