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1.
Article in Chinese | WPRIM | ID: wpr-907722

ABSTRACT

Objective:To explore the predictive value of Red Blood Cell Distribution Width (RDW) in predicting the prognosis of patients with Extracorporeal Membrane Oxygenation (ECMO).Methods:The clinical data of patients undergoing ECMO admitted to Intensive Care Unit of Sichuan Provincial People’s Hospital from January 2015 to January 2020 were retrospectively analyzed. Patients were divided into the survival group and death group according to the prognosis during ICU hospitalization. The patients' basic data , acute physiology and chronic health score system Ⅱ (APACHE Ⅱ), RDW and activated partial thromboplastin time (APTT) at 72 hours after treatment with ECMO were compared between the two groups. Univariate and Logistic regression multivariate analyses were used to analyze the prognostic factors of patients with ECMO, predictive models and death warning scores were established. The receiver operating characteristic (ROC) curve was plotted to evaluate the diagnostic efficacy of RDW and death warning scores for the prognosis of patients with ECMO.Results:A total of 71 patients with ECMO who met the inclusion criteria were included, including 38 patients in the death group and 33 patients in the survival group. The age, APACHE-Ⅱscore, 72 h RDW and 72 h APTT in the death group were higher than those in the survival group. Respectively, the hospitalization time of ICU in the death group was significantly lower than that in the survival group ( P< 0.05). Logistic regression analysis showed that APACHE-Ⅱscore ( OR=1.117, P=0.047)、72 h RDW( OR=1.102, P=0.029) and 72 h APTT ( OR=1.049, P=0.029) were independent risk factors for death in patients with ECMO. ROC curve analysis showed that the area under ROC curve (AUC) of the APACHE-Ⅱ, score 、72 h RDW and 72 h APTT were 0.691, 0.691 and 0.632( P<0.05), Respectively, the combined AUC was 0.764, the sensitivity was 0.526, and the specificity was 0.909. The death warning score of patients with ECMO was established according to the Predictive model , which is less than 2 points with low risk of death and more than 2 points with high risk of death. The area under the ROC curve of death warning score is 0.8, the sensitivity is 0.607 and the specificity is 0.923. Conclusions:The RDW at 72 hours after treatment with ECMO has a good value in predicting the prognosis of patients with ECMO. Besides, a greater predictive value for the prognosis of patients with ECMO by combining 72 hours RDW, 72 hours APTT with APACHE-Ⅱscore than that of any separate indicator.

2.
Article in Chinese | WPRIM | ID: wpr-906232

ABSTRACT

Objective:To investigate the mechanism of Xiangsha Liujunzi Tang in improving liver lipid deposition in ApoE<sup>-/-</sup> atherosclerotic (AS) mice by affecting long noncoding RNA-HC (Lnc-HC)/microRNA-130b (miR-130b) in the regulation of cholesterol metabolism. Method:Totolly 10 C57BL/6J mice were selected as normal controls, and 30 healthy ApoE<sup>-/-</sup> mice fed with high fat diet for 12 weeks were then randomly divided into the model group, Xiangsha Liujunzi Tang group(19.12 g·kg<sup>-1</sup>·d<sup>-1</sup>) and simvastatin group(2.275 mg·kg<sup>-1</sup>·d<sup>-1</sup>), with gavage administration for 4 weeks. The serum lipid level of mice was detected by automatic biochemistry analyzer, and the histopathological changes of liver cells were observed by hematoxylin-eosin (HE) staining. Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) was used to detect expression of long noncoding RNA-HC, and miR-130b. Real-time PCR and Western blot assay were used to detect gene and protein expression of peroxisome proliferator-activated receptor gamma (PPAR<italic>γ</italic>), liver X receptor (LXR), ATP-binding cassette transporters A1 (ABCA1), ATP-binding cassette transporters G1 (ABCG1), ATP-binding cassette transporters G5 (ABCG5), and ATP-binding cassette transporters G8 (ABCG8). Result:Compared with the normal control group, the mice in the model group showed abnormal blood lipids, larger liver cells, obvious fat vacuoles, significantly increased expression of Lnc-HC, miR-130b in liver, and significantly decreased gene and protein expression of PPAR<italic>γ</italic>, LXR, ABCA1, ABCG1, ABCG5, and ABCG8 in mice liver (<italic>P</italic><0.05,<italic>P</italic><0.01). Compared with the model group, the abnormal blood lipid levels of the mice in the Xiangsha Liujunzi Tang group and the simvastatin group were improved, and the number of fatty vacuoles of liver cells was significantly reduced, the expression of liver Lnc-HC, miR-130b in Xiangsha Liujunzi Tang group decreased significantly (<italic>P</italic><0.05,<italic>P</italic><0.01), the gene and protein levels of liver PPAR<italic>γ</italic>, ABCA1, ABCG1, ABCG5, ABCG8 in mice of the Xiangsha Liujunzi Tang group and the simvastatin group showed an upward trend. Among them, the gene and protein expression of LXR protein in the liver of the Xiangsha Liujunzi Tang group was significantly up-regulated (<italic>P</italic><0.05). Conclusion:Xiangsha Liujunzi Tang may improve the lipid deposition in the liver of ApoE<sup>-/- </sup>AS mice by affecting Lnc-HC/miR-130b to regulate the cholesterol metabolism process mediated by PPAR<italic>γ</italic>, thus playing a role in preventing and treating AS.

3.
Article in Chinese | WPRIM | ID: wpr-921817

ABSTRACT

Tongsaimai Tablets/Capsules are composed of Lonicerae Japonicae Flos, Angelicae Sinensis Radix, Achyranthis Bidentatae Radix, Codonopsis Radix, Dendrobii Caulis, Astragali Radix, Scrophulariae Radix, and Glycyrrhizae Radix et Rhizoma, and are effective in promoting blood circulation, removing blood stasis, supplementing Qi, and nourishing Yin. It is widely used in the treatment of peripheral vascular diseases. With 40 years of clinical application, it has accumulated substantial research data and application experience. Its good clinical efficacy and pharmacoeconomic benefits in improving the clinical symptoms of peripheral vascular diseases have been confirmed by relevant research. Meanwhile, this drug has also been recommended by many expert consensus, guidelines, and teaching materials, serving as one of the most commonly used Chinese patent medicines in clinical practice. To further improve the understanding of the drug among clinicians and properly guide its clinical medication, the China Association of Chinese Medicine took the lead and organized experts to jointly formulate this expert consensus. Based on the questionnaire survey of clinicians and the systematic review of research literature on Tongsaimai Tablets/Capsules with clinical problems in the PICO framework, the consensus, combined with expert experience, concludes recommendations or consensus suggestions by GRADE system with the optimal evidence available through the nominal group technique. This consensus defines the indications, usage, dosage, course of treatment, medication time, combined medication, and precautions of Tongsaimai Tablets/Capsules in the treatment of peripheral vascular diseases, and explains the safety of its clinical application. It is recommended for clinicians and pharmacists in the peripheral vascular department(vascular surgery), traditional Chinese medicine surgery(general surgery), and endocrinology department of hospitals at all levels in China.


Subject(s)
Capsules , Consensus , Drugs, Chinese Herbal , Humans , Medicine, Chinese Traditional , Peripheral Vascular Diseases , Tablets
4.
Article in Chinese | WPRIM | ID: wpr-884813

ABSTRACT

Objective:To investigate the distribution of 99Tc m-methylene diphosphonate (MDP) at different stages of bone injury repair. Methods:A total of 30 rabbit models of femur injury were established by the method of electric drill and perforation of femur. According to the different stages of bone injury repair (at 1, 2 and 3 week), rabbits were divided into group A, B and C ( n=10 each group). Femoral SPECT/CT imaging was performed on the last day of different stages of bone injury repair to obtain radioactivity counts in the region of interest (ROI) on the test side and control side and to calculate target/background ratio (T/B). The light intensity of 3 groups was analyzed by phosphor screen imaging and the distribution of 99Tc m-MDP in bone cells was observed by autoradiography. One-way analysis of variance and paired t test were used to analyze the data. Results:The T/B values of group A, B and C were 1.16±0.14, 1.39±0.23 and 1.18±0.10, respectively ( F=5.83, P<0.01). There were significant differences of the maximum radiation count between the test side (50.00±12.45, 59.50±12.83 and 55.10±9.26) and the control side (43.20±9.57, 50.00±12.30 and 44.30± 6.50) in group A, B and C ( t values: 3.24, 2.28 and 5.77, all P<0.05). There were significant differences in the light intensity of bone specimens in group A, B and C by phosphor screen imaging (37 324.67±6 481.50, 60 950.33±9 781.72 and 43 905.00±4 957.92; F=8.25, P=0.02). 99Tc m-MDP were deposited in both intracellular and extracellular during different stages of bone repair in osteocytes and osteoblasts under autoradiography. Conclusion:At different stages of bone injury repair, the concentration of 99Tc m-MDP is significantly distributed, suggesting that there are other ways of concentration mechanism of 99Tc m-MDP in bone tissue besides the chemical adsorption with hydroxyapatite.

5.
Article in Chinese | WPRIM | ID: wpr-860876

ABSTRACT

Objective To compare breast radiation dose between contrast-enhanced mammography (CEM) and full-field digital mammography (FFDM), and to explore the correlation of breast radiation dose with gland compression thickness. Methods The average gland dose (AGD) of FFDM and CEM were compared through phantom experiment on the same mammography device. Totally 102 patients with breast disease underwent CEM (CEM group), while other 104 underwent FFDM (FFDM group) on the same machine, and AGD and gland compression thickness were recorded. Based on the 5th breast imaging-reporting and data system (BI-RADS) of American college of radiology (ACR), the breasts were divided into dense glands and non-dense glands. The differences of AGD and gland compression thickness were compared between 2 groups and among different types of glands, and the correlations of AGD and gland compression thickness were analyzed. Results AGD of low-energy CEM and FFDM were similar in phantom experiment. There were 22 non-dense glands and 80 dense glands in CEM group, 39 non-dense glands and 65 dense glands in FFDM group. No significant difference in gland compression thickness was found between 2 groups (U=4 823.50, P>0.05). There was no statistical difference of the AGD in CEM group and AGD of FFDM group (U=5 249.50, P>0.05), while AGD of low energy CEM was lower than that of FFDM group (U=2 111.00, P0.05). The AGD of dense glands was higher than that of non-dense glands in FFDM group (U=846.50, P0.05). Conclusion The overall ADC of CEM was comparable to that of FFDM. The thinner the gland compression thickness is, the lower the radiation dose is. CEM had more advantageous for dense breasts than for non-dense glands.

6.
Article in Chinese | WPRIM | ID: wpr-843254

ABSTRACT

Objective: To investigate the feasibility of single-port video-assisted thoracoscopic surgery (VATS) for pulmonary sequestration. Methods: Fifteen cases with intralobar pulmonary sequestration in Renji Hospital, Shanghai Jiao Tong University School of Medicine from January 2014 to December 2018 were reviewed. All the cases had underwent lobectomy by single-port VATS, including 13 cases of left lower lobectomy and 2 cases of right lower lobectomy. Results: Pleural adhesion was found in 11 of the 15 cases. Lobectomy was successfully performed by single-port VATS in 12 cases. Three cases were transferred to multi-port VATS because of severe pleural adhesion. There was no case conversed to thoracotomy in the study. There was no mortality and complication during the perioperative period. For the 15 cases, the operation time was (96.7±27.8) min, the bleeding volume was (75.3±45.5) mL, the postoperative intubation was (5.2±2.0) d and the postoperative hospital stay was (6.2±2.2) d. Conclusion: Single-port VATS treatment for pulmonary sequestration is safe, effective and feasible.

7.
Journal of Medical Postgraduates ; (12): 220-224, 2020.
Article in Chinese | WPRIM | ID: wpr-818407

ABSTRACT

Silica Nanoparticles (SiNPs) is widely used in many fields including antibacterial agent, molecular probe and drug delivery forits special physicochemical properties. Recently, the biosecurity of nano-materials has become a hot spot of research in toxicology due to special structure and functional activeness of nano-materials. This paper briefly summarized recent researchreports at home and abroadto review the toxicity of SiNPs on cells and animals, the factors for influencing the toxicity of SiNPs and the mechanisms underlying SiNPs biotoxicity, aiming to provide references for the development, application and biosecurity of SiNPs.

8.
Article in Chinese | WPRIM | ID: wpr-810543

ABSTRACT

In this study, 1 065 infants and young children aged 24 months below in ethnic minorities gathering in poor rural areas in poor rural areas of Liangshan Yi Autonomous Prefecture of Sichuan Province and Gannan Tibetan Autonomous Prefecture of Gansu Province were investigated for their anemia status from October to November 2014, and the association between anemia and the utilization of maternal and child health services was analyzed. The prevalence of anemia in this area was 52.68%(561/1 065). After the adjustment of socio-demographic characteristics of mothers and infants, compared with infants aged 2-5 months, Han ethnic group, and infants whose mother was not anemic, the OR(95%CI) values of infant anemia for infants aged 6-12 months, 13-8 months, 19-24 months, ethnic minorities group, and infants whose mother was anemic were 11.65 (7.09-19.14), 9.91 (5.99-16.38), 5.87 (3.39-10.16), 1.55 (1.10-2.18) and 1.52 (1.14-2.04), respectively; Compared with infants whose child examination times not up to standard, and who were not only non-hospital delivered but also received inadequate number of inoculation, the OR (95%CI) values of infant anemia for infants whose child examination times up to standard, and who were not only hospital delivered but also received adequate number of inoculation were 0.60 (0.38-0.94) and 0.71 (0.52-0.98), respectively. The infants anemia is associated with the utilization of maternal and child health services.

9.
Article in Chinese | WPRIM | ID: wpr-774541

ABSTRACT

This research aims to develop an UHPLC method, based on core-shell column(i.e. superficially porous particles), for simultaneous determination of eight isoflavonoids including formononetin,(6αR,11αR)-3-hydroxy-9,10-dimethoxypterocarpan, calycosin-7-O-β-D-glucopyranoside,(3R)-7,2-dihydroxy-3,4-dimethoxyisoflavone, calycosin, ononin,(6αR,11αR)-9,10-dimethoxypterocarpan-3-O-β-D-glucopyranoside, and(3R)-7,2-dihydroxy-3,4-dimethoxyisoflavan-7-O-β-D-glucopyranoside in Astragali Radix. The analysis was performed on an Agilent Poroshell EC-C_(18 )column(2.1 mm×100 mm, 2.7 μm) with 0.2% formic acid solution(A)-acetonitrile(B) as mobile phase for gradient elution. The flow rate was 0.5 mL·min~(-1), with column temperature of 40 ℃ and the wavelengths were set at 260 and 280 nm. According to the results, all calibration curves showed good linearity(R~2>0.999 8) within the tested concentration ranges. Both the intra-and inter-day precisions for 8 isoflavonoids were less than 0.80%, with the mean recovery at the range of 94.71%-104.6%. Thus, the newly developed UHPLC method using core-shell column owned the advantages in terms of rapid analysis, low column pressure and less solvent consumption, thus enabling the usage of conventional HPLC systems. Meanwhile, quantitative evaluation was carried out for 22 batches of commercial Astragali Radix. It has been found that great variations occurred for the content of the individual isoflavonoids among different batches; in contrast, the total content of total 8 isoflavonoids(>0.1%) was stable in most samples, indicating that it was reasonable to involve all isoflavonoids as the chemical markers for the quality control of Astragali Radix.


Subject(s)
Astragalus Plant , Chemistry , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Reference Standards , Flavones , Phytochemicals , Plant Roots , Chemistry , Quality Control
10.
Article in Chinese | WPRIM | ID: wpr-742899

ABSTRACT

Under normal physiological conditions, hemostatic process is a dynamic balance process dependent on many factors.It involves interaction between primary hemostasis (vasoconstriction, platelet clot formation), secondary hemostasis (thrombin generation, fibrin polymerization) and fibrinolysis, and any abnormalities of the three may cause hemorrhagic or thrombotic diseases.Conventional coagulation tests are useful for a limited degree since they only reflect a portion of the coagulation cascade.Thromboelastography (TEG) provides a more complete picture of coagulation status, taking into account more factors involved in the clotting process, including platelet activity and clotting factors, which has been widely used in clinic.In this article, we will briefly discuss clinical applications and new development of TEG.

11.
Article in Chinese | WPRIM | ID: wpr-850739

ABSTRACT

Objective: To establish the fingerprint of Buyang Huanwu Decoction (BHD) based on core-shell column for its quality control. Methods: The extract of BHD was separated on an Agilent Poroshell 120 SB-C18 (150 mm × 4.6 mm, 2.7 μm) with a gradient elution. The mobile phase consisted of acetonitrile-0.1% formic acid. The detection wavelength was 280 nm. The similarity evaluation of 15 batches of BHD was carried out by the “Similarity Evaluation System for Chromatographic Fingerprints of TCM”. HPLC-Q-Orbitrap HRMS/MS was used to characterize the 21 chemical compounds of the extract of BHD. Results: The chromatographic fingerprints of 15 batches of BHD were generated 21 common peaks (P1-P21), belonging to all seven medicinal herbs in 38 min. Methyl quinate (P4), calycosin-7-O-β-D-glucoside (P11), calycosin-7-O-β-D-glucoside-6″-O-malonate (P13), ononin (P15), (6αR, 11αR)-9,10-dimethoxypterocarpan-3-O-β-D-glucoside (P16), isomucronulatol-7-O-β-D-glucoside (P17), calycosin (P18), formononetin (P19), (6αR,11αR)-9,10-dimethoxypterocarpan (P20), and isomucronulatol (P21) were from Astragali Radix. Succinyl adenosine (P3), 6-hydroxykaempferol-3,6-diglucoside (P5), and kaempferol-3-O-sophoroside (P10) were from Carthami Flos. Gallic acid (P2) and paeoniflorin (P9) were from Paeoniae Radix Rubra. Vanillic acid (P7) was from Angelicae Sinensis Radix. Guanoside (P1) was from Pheretima. Amygdalin (P6) and prunasin (P8) were from Persicae Semen. Ferulic acid (P12) and senkyunolide H (P14) were from Angelicae Sinensis Radix and Chuanxiong Rhizoma. A total of 21 components were identified by UHPLC-Q-Orbitrap HRMS/MS. It was the first time to establish the UHPLC fingerprint of BHD by core-shell chromatography technology. Conclusion: The method is simple and accurate with a good reproducibility and time consuming, which will provide a basis for the further research on effective constituents in BHD.

12.
Article in Chinese | WPRIM | ID: wpr-818896

ABSTRACT

Objective To understand the current situation of soil-transmitted nematode infections and influencing factors of pupils in rural areas of Yi Ethnicity in Butuo County, Sichuan Province, so as to provide the evidence for the prevention and control of soil-transmitted nematode infections. Methods In 2016, the pupils of 2 villages in Butuo County were selected by the multi-stage random sampling method, and the relevant information was obtained by questionnaires, including social demographic information, diet and hygiene habits and acknowledge of parasitic disease prevention. The soil-transmitted nematode infections were examined by Kato-Katz technique. The results were statistically analyzed by the single factor analysis and multivariate logistic regression methods. Results A total of 160 pupils were investigated in this survey. The soil-transmitted nematodes included Ascaris lumbricoides, Trichuris trichiura and hookworm. The overall infection rate was 89.38%, and the multi-infection rate was 54.55%. The infection rates of A. lumbricoides and T. trichiura were higher. The infection densities were mild (75.68%) or moderate (22.97%). The logistic regression analysis showed that the protective factor of soil-transmitted nematode infections was the knowledge about correct diet and health habits notified by teachers (OR = 0.67), but the risk factor was drinking unboiled water (OR = 19.26). Conclusions The infection rate of nematodes is still high in the pupils in rural areas of Yi Ethnicity in Butuo County, Sichuan Province. Therefore, we should strengthen the prevention and control of soil-transmitted nematode infections, especially A. lumbricoides and T. trichiura infections, and mostly develop the teachers’function in health education.

13.
Article in Chinese | WPRIM | ID: wpr-818774

ABSTRACT

Objective To understand the current situation of soil-transmitted nematode infections and influencing factors of pupils in rural areas of Yi Ethnicity in Butuo County, Sichuan Province, so as to provide the evidence for the prevention and control of soil-transmitted nematode infections. Methods In 2016, the pupils of 2 villages in Butuo County were selected by the multi-stage random sampling method, and the relevant information was obtained by questionnaires, including social demographic information, diet and hygiene habits and acknowledge of parasitic disease prevention. The soil-transmitted nematode infections were examined by Kato-Katz technique. The results were statistically analyzed by the single factor analysis and multivariate logistic regression methods. Results A total of 160 pupils were investigated in this survey. The soil-transmitted nematodes included Ascaris lumbricoides, Trichuris trichiura and hookworm. The overall infection rate was 89.38%, and the multi-infection rate was 54.55%. The infection rates of A. lumbricoides and T. trichiura were higher. The infection densities were mild (75.68%) or moderate (22.97%). The logistic regression analysis showed that the protective factor of soil-transmitted nematode infections was the knowledge about correct diet and health habits notified by teachers (OR = 0.67), but the risk factor was drinking unboiled water (OR = 19.26). Conclusions The infection rate of nematodes is still high in the pupils in rural areas of Yi Ethnicity in Butuo County, Sichuan Province. Therefore, we should strengthen the prevention and control of soil-transmitted nematode infections, especially A. lumbricoides and T. trichiura infections, and mostly develop the teachers’function in health education.

14.
National Journal of Andrology ; (12): 226-230, 2018.
Article in Chinese | WPRIM | ID: wpr-689772

ABSTRACT

<p><b>Objective</b>To compare the clinical effects and postoperative complications of microsurgical subinguinal varicocelectomy (MSV) with or without delivery of the testis and ligation of gubernacular veins in the treatment of varicocele.</p><p><b>METHODS</b>We retrospectively analyzed the clinical data about 163 varicocele patients treated by MSV, 40 with (group A) and the other 123 without delivery of the testis and ligation of gubernacular veins (group B). We compared the operation time, postoperative complications, rate of recurrence, and semen parameters before and at 3 months after surgery between the two groups of patients.</p><p><b>RESULTS</b>The operation time was significantly longer in group A than in B ([81.1 ± 20.0] vs [62.3 ± 9.6] min, P = 0.041). Sperm concentration, total sperm count per ejaculate, sperm viability, and the percentage of progressively motile sperm were significantly improved in both groups at 3 months after MSV as compared with the baseline (P < 0.05). There were no statistically significant differences in the above semen parameters between the two groups of patients with grade Ⅲ varicocele before and after surgery (P < 0.05). Scrotal edema developed in 5 cases in group A and wound infection in 2 cases in group B after MSV, but no postoperative testicular atrophy or recurrence was observed in either of the two groups.</p><p><b>CONCLUSIONS</b>MSV with delivery of the testis and ligation of gubernacular veins showed no advantages over that without in reducing varicocele recurrence and improving semen parameters, but rather involved longer operation time and a higher incidence rate of postoperative complications.</p>


Subject(s)
Edema , Humans , Ligation , Male , Microsurgery , Methods , Operative Time , Postoperative Complications , Recurrence , Retrospective Studies , Semen , Semen Analysis , Sperm Count , Spermatozoa , Testis , Treatment Outcome , Varicocele , General Surgery , Vascular Surgical Procedures , Methods , Veins , General Surgery
15.
Article in Chinese | WPRIM | ID: wpr-695712

ABSTRACT

Various forms of noninvasive respiratory support have been applied to the treatment of infant respiratory distress and hypoxemia.The most common noninvasive respiratory support in neonatal intensive care unit is nasal continuous positive airway pressure (NCPAP).But the NCPAP systems are not always well accepted by the neonatal population,with the risk of mucosal injury and nosocomial infection.In recent years,humidified high-flow nasal cannula (HHFNC) has been introduced and developed as a possible alternative to NCPAP for noninvasive respiratory support mode,because it increases patients' comfort and the effectiveness of the ventilation.This article summarized the current research progress of HHFNC therapy in pediatric patients.

16.
Article in Chinese | WPRIM | ID: wpr-695643

ABSTRACT

Elevated low density lipoprotein cholesterol (LDL-C) is a major risk factor for cardiovascular disease.Studies show that proprotein convertase subtilisin/kexin type 9 (PCSK9) is a circulation enzyme and serves a pivotal function in the degradation of low density lipoprotein receptor,which contributes to the decrease in hepatic cholesterol uptake and increase in circulating LDL-C.PCSK9 inhibitor can significantly elevate the surface of low density lipoprotein receptor of liver cells and bond more LDL-C to decrease the level of LDL-C.Thus PCSK9 has emerged as a popular target for the development of new cholesterol lowering drugs and therapeutic intervention of cardiovascular disease.In this article,the history,mechanism of action,metabolic effects of PCSK9 and the clinical outcomes of PCSK9 inhibitors will be briefly reviewed.

17.
Article in Chinese | WPRIM | ID: wpr-695623

ABSTRACT

Atherosclerosis is an immune-mediated inflammatory disease of the arterial wall,with both the innate and adaptive immune systems responding to many endogenous and exogenous antigens.Both proatherogenic as well as atheroprotective roles have been identified for the immune system in atherosclerosis.Hence,it is conceivable that an immuno-modulatory strategy via active immunization against many of these antigens could potentially alter the natural history of atherosclerosis.Cholesterol ester transfer protein (CETP) plays an important role in lipid metabolism,suggesting it might prevent atherosclerosis.This review discusses the recent advances of vaccine targeting the development of atherosclerosis,mainly about the CETP targeting vaccines.

18.
Article in Chinese | WPRIM | ID: wpr-692673

ABSTRACT

Objective Broth dilution method was used as a reference method to observe the capability of Kirby-Bauer disc diffusion assay(K-B)for correcting automated ampicillin susceptibility detection of He-mophilus influenzae(HI).Methods A total of 228 HI strains isolated were collected,broth dilution assay,K-B and automated microdilution broth test(ATB)were used to determine the susceptibility of HI to ampicillin. Analyze the essential agreements of the three methods and the correction of K-B to the errors of A TB. Results The essential agreement of K-B or ATB with broth dilution method were 77.19%,70.18% respec-tively,combination of K-B and ATB could make the essential agreement increase up to 86.0%,which was sig-nificantly higher than ATB(χ2=16.600,P=0.000).Major error of ATB(42.0%)was higher than that of K-B(10.0%)(χ2=13.306,P=0.001),but very major error and minor error showed no significant difference be-tween the two methods(χ2=1.208,P=0.272;χ2=1.182,P=0.227),meanwhile,76.19% of major error of ATB could be corrected by K-B.For the very major error of ATB,53.57% could be corrected by K-B.Howev-er,the corrective capability of K-B to minor error of ATB was relative low.Conclusion K-B test could correct some errors generated by ATB.For the β-lactamase negative strains which were judged as ampicillin resistance by A TB,K-B test should be used to correct the errors by ATB.Moreover,it is necessary to apply K-B to confirm am-picillin sensitivity of the β-lactamase positive strains which were judged as ampicillin susceptible by ATB.

19.
Article in Chinese | WPRIM | ID: wpr-699596

ABSTRACT

Objective To evaluate the clinical effect of femtosecond laser assisted cataract surgery with Toric IOL implantation on correcting astigmatism and IOL rotating stability.Methods A prospective,nonrandomized,controlled study was conducted in 68 patients (82 eyes) diagnosed as age-related cataract with more than 1.0 diopter (D) regular astigmatism,including 41 eyes undergoing Toric IOL implantation with femtosecond laser assisted cataract surgery,and the other eyes receiving Toric IOL with phacoemulsification.And observation was performed for the uncorrected visual acuity (UCVA) before operation and 6 months after operation,preoperative corneal astigmatism,anticipated and postoperative residual astigmatism 6 months after operation.Then,comparison was performed for the degree of Toric lens axis rotation,as well as the horizontal and vertical movement of the IOL and the degree of vertical deflection in the capsule at 1,3,and 6 months after operation.Results In the observation group,preoperative UCVA (logMAR),preoperative corneal astigmatism,postoperative BCVA and the residual astigmatism at 6 months after operation was 1.03 ± 0.32,(2.09 ± 0.73) D,0.13 ± 0.11 and (0.33 ± 0.27) D,respectively,while the corresponding data in the control group was 0.92 ± 0.40,(1.69 ± 0.64) D,0.13 ± 0.09,(0.26 ± 0.22) D;the postoperative visual acuity in both groups was significantly improved,and residual astigmatism was significantly decreased at 6 months after operation (all P < 0.001).There was no significant difference in Toric lens axis after femtosecond laser assisted surgery and phacoemulsification at 1 month,3 months,6 months after operation (all P > 0.05),but the differences in the IOL movement in horizontal and vertical direction were statistically significant at the different time-points (all P < 0.05),and there was significant differences in the degree of vertical deflection (all P < 0.05).Conclusion Both femtosecond laser assisted cataract surgery and phacoemulsification combined with Toric IOL can achieve a better stability and predictability.

20.
Article in Chinese | WPRIM | ID: wpr-776581

ABSTRACT

OBJECTIVE@#To study the effects and mechanisms of curcumin alleviating oxidative stress induced by overtraining and inhibiting renal apoptosis in rats.@*METHODS@#Male Wistar rats of 7 weeks old were divided into control group (C group, 12), overtraining group (OM group, 11), curcumin + overtraining group (COM group, 14). Group C did not undergo any exercise intervention. Rats in OM group and COM group underwent 8-week incremental load swimming training. During the training, the COM group was treated with curcumin at the dose of 200 mg/(kg·d) in the volume as 5 ml/kg by intragastric administration, and the other groups was treated with an equal volume of 0.5% carboxymethylcellulose. Twenty-four hours after the last training, renal histopathological changes were observed by light microscopy, related biochemical indicators in blood and renal tissue were detected.@*RESULTS@#The results showed that after 8 weeks of incremental load swimming training, the renal tissue structure of group C was normal under light microscope; histopathological changes were observed in OM group; COM group was significantly relieved compared with OM group. Compared with group C, serum levels of corticosterone (Cor), creatinine (Cr) and blood urea nitrogen (BUN) in OM group were increased (0.05), while the expression of heme oxygenase-1 (HO-1) was decreased (<0.05), total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity were decreased (<0.01), malondialdehyde (MDA) concentration was increased (<0.01); the renal apoptosis was increased (<0.01), the expression of anti-apoptotic B cell lymphoma-2 protein (Bcl-2) was decreased (<0.01), and the expression of proapoptotic Bcl-2 associated X protein (Bax) was increased (<0.01). Compared with the OM group, Cor level was decreased (<0.01) in the COM group, T level was increased (<0.01), Cr and BUN levels were lower (<0.05); the expression of Nrf2 and HO-1 were increased (<0.05), T-AOC and SOD activity were increased (<0.01), MDA concentration was decreased (<0.05); the renal apoptosis was decreased (<0.05), the expression of Bcl-2 was increased (<0.05), and the expression of Bax was decreased (<0.01). The trend of testosterone/corticosterone ratio between groups was consistent with testosterone change, and the change trend of Bcl-2/Bax ratio was consistent with the change of Bcl-2.@*CONCLUSIONS@#The 8-week incremental load swimming training triggered excessive training in rats, aggravated oxidative stress and accelerated renal apoptosis, leading to pathological changes and dysfunction of kidney. Curcumin can up-regulate expression of Nrf2 and HO-1, effectively alleviates oxidative stress induced by overtraining, thereby increasing Bcl-2 expression, decreasing Bax expression, inhibiting renal apoptosis and protecting renal tissue structure and function properly.


Subject(s)
Animals , Apoptosis , Curcumin , Kidney , Male , Oxidative Stress , Rats , Rats, Wistar
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