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1.
China Pharmacy ; (12): 378-384, 2022.
Article in Chinese | WPRIM | ID: wpr-913099

ABSTRACT

The volatile oil of Magnolia liliflora ,as the main active part of the medicinal value ,has rich chemical constituents , mainly including cineole ,farnesol,α-pinene,β-pinene,terpineol,cadinene,linalool,citronellol,camphor. It has a wide range of pharmacological effects ,mainly anti-inflammatory and anti-allergic effects. Clinically ,it is mainly used to treat acute and chronic rhinitis,allergic rhinitis and sinusitis. This paper summarizes the research reports on the volatile oil of M. liliflora at home and abroad in the past 10 years,compares the chemical constituents of the volatile oil from M. liliflora under different conditions (origin,variety,part and extraction method ),combs its pharmacological effects and clinical application status ,and looks forward to its development prospect ,in order to provide reference for the further development of the medicinal value of the volatile oil from M. liliflora .

2.
China Pharmacy ; (12): 800-807, 2022.
Article in Chinese | WPRIM | ID: wpr-923184

ABSTRACT

OBJECTIVE To study the composition of chemical constituents of Sargassum fusiforme and its in vitro anti- neuroinflammatory activity ,and to provide reference for its development and utilization and the study of pharmacodynamic substances. METHODS UHPLC-QTOF-MS/MS analysis method and GC-MS/MS method were used to analyze the chemical constituents of S. fusiforme . The lipopolysaccharide (1 μg/mL)was adopted to establish the inflammatory model of neuromicroglia BV2. Using paroxetine (5 μg/mL)as positive control ,CCK-8 assay was used to detect the effects of the extracts of S. fusiforme (20,40,60,80,100 μg/mL)on the activity and morphology of neuromicroglia BV 2. The effects of the extracts of S. fusiforme (40,60,80 μg/mL)on the contents of tumor necrosis factor α(TNF-α)and interleukin- 6(IL-6)in cell supernatant were detected by ELISA. RESULTS A total of 103 non-volatile constituents were identified by UHPLC-QTOF-MS/MS ,and 60 volatile constituents were obtained by GC-MS/MS. The extracts of S. fusiforme (40,60,80 μ g/mL) could significantly reduce the abnormally increased activation of neuromicroglia BV 2 and the contents of TNF-α and IL-6 due to lipopolysaccharide (P<0.05 or P<0.01). CONCLUSIONS The study establish the full spectrum of chemical constituents of S. fusiforme ,and it is confirmed that fusiforme has certain in vitro anti-neuroinflammatory activity.

3.
Acta Pharmaceutica Sinica ; (12): 460-466, 2022.
Article in Chinese | WPRIM | ID: wpr-922922

ABSTRACT

Based on the similar structure of adrenaline shared by higenamine (HI), salsolinol (SA) and coryneine (CO), a photochemical colorimetric sensor based on the displacement reaction of o-diphenol hydroxyl group and alizarin red S-phenylboric acid system was constructed to quickly distinguish and identify the cardiac strength of Shengfupian. The results show that the optimal condition of the sensor is: the molar ratio of alizarin red S (ARS) to phenylboric acid (PA) is 1∶3, reaction temperature is 0 ℃; The preparation method of the sample solution is optimized as follows: 2.5 g of Shengfupian powder was taken, 10 times the amount of methanol was added, and 300 W, 40 kHz ultrasound was carried out for 15 min; methodological studies showed that the method had good precision, repeatability and stability. The |△G| value (G is green, |△G| = |G after - G before|) of each sample was obtained by response values determination of 14 batches of Shengfupian. LC-MS/MS was used to determine the contents of three cardiac components in Shengfupian. It was found that the order of the total contents of cardiotonic components was basically consistent with |△G|. Then the correlation was analyzed, and the correlation coefficient R2 was as high as 0.87, which proved the scientificity and accuracy of this method. This study fills the methodological gap of rapid evaluation of the quality of Shengfupian, and provides the key technical support for the high quality and good price of Shengfupian in the market circulation and clinical application.

4.
Acta Pharmaceutica Sinica ; (12): 331-342, 2022.
Article in Chinese | WPRIM | ID: wpr-922912

ABSTRACT

Ionic liquids (ILs) are liquid mixtures formed by anions and cations in a certain stoichiometric ratio under certain conditions. They are widely used in various fields because of their simple preparation process, low volatility, high stability, high conductivity and non-flammability. Here, we firstly introduce their formation principles, classification, and physical and chemical properties in detail. Then, we summarize their functions in pharmaceutical preparations, such as improving the solubility of insoluble drugs, enhancing the stability of drugs, and promoting the permeability of drugs, as well as their role as active pharmaceutical ingredients (API) to fabricate new drug delivery systems of API-ILs. Finally, we reviewed the applications of ILs in different administration routes, including oral, transdermal, mucosal, and injection routes, and meanwhile offer perspectives for the further use of ILs.

5.
Experimental Neurobiology ; : 387-400, 2021.
Article in English | WPRIM | ID: wpr-914310

ABSTRACT

Insomnia has become a common central nervous system disease. At present, the pathogenesis of insomnia is not clear. Animal models can help us understand the pathogenesis of the disease and can be used in transformational medicine. Therefore, it is very necessary to establish an appropriate model of insomnia. Clinical data show that insomnia patients with high levels of thyroxine and often accompanied by cardiovascular problems, a common mechanism underlying all of these physiological disruptions is the sympathetic nervous system. Combined with the characteristics of chronic onset of clinical insomnia, an insomnia model induced by long-term intraperitoneal injection of thyroid hormone has been created in our laboratory. In this paper, the insomnia-like state of the model was evaluated based on three validity criteria. Face validity has been demonstrated in metabolism, the Morris water maze, electrocardiogram (ECG) and electroencephalogram (EEG). Structure validity has been proved by the results of targeted metabolomics. After treatment with diazepam, a commonly used clinical anti-insomnia drug, the above physiological and pathological disorders were reversed. The results of comprehensive analysis show that the established thyrotoxicosis-associated insomnia model meets the validity requirement to establish an appropriate animal model of insomnia. The model presented in this article might help to study pathogenetic mechanisms of clinical insomnia, as well as to test promising methods of insomnia treatment.

6.
Cancer Research and Clinic ; (6): 801-804, 2021.
Article in Chinese | WPRIM | ID: wpr-912971

ABSTRACT

The incidence of early-onset colorectal cancer is increasing year by year, and it occupies an important position in colorectal cancer. Most of the characteristics of patients with early-onset colorectal cancer are different from those with late-onset colorectal cancer, the patients are diagnosed in a late stage, and their pathological manifestations show a type of poor prognosis. At present, there is a lack of consensus and guideline for the diagnosis, treatment and screening of early-onset colorectal cancer. A full understanding of the characteristics of early-onset colorectal cancer helps to understand its pathogenesis and guide screening and treatment to reduce the incidence and mortality of colorectal cancer. This article reviews the clinicopathological characteristics, molecular pathological mechanisms, diagnosis and treatment, and prognosis of early-onset colorectal cancer, in order to provide references for elucidating its etiology and formulating scientific and standardized screening strategies.

7.
Article in Chinese | WPRIM | ID: wpr-912293

ABSTRACT

Objective:The study aimed to evaluate the effect of systemic-pulmonary shunt(SPS) on the infants with tetralogy of Fallot(TOF) with hypoplasia pulmonary arteries.Methods:Among 949 infants less than three-year-old who underwent surgical intervention between January 2010 and December 2015, 853 infants underwent one-stage primary repair(group Ⅰ), whereas 96 infants underwent SPS(group Ⅱ). Among them, 546 were males and 403 were females. The average age and average weight at operation were 13 months and 9.4 kg, respectively. 20.8% infants had clinical symptoms before operation. Perioperative and follow-up parameters were assessed including age, weight, sex, transcutaneous oxygen saturation(SPO 2), preoperative clinic symptom, ratio of McGoon, NAKATA index, Z score of pulmonary annulus, left ventricular end diastolic diameter(LVEDD). Results:There were 28(3.0%) early postoperative deaths in the whole group. And no significant difference in mortality was found between group Ⅰ(2.9%) and group Ⅱ(3.1%). Compared with group Ⅰ, age and weight were significantly lower in patients in group Ⅱ[(0.95±0.56)years old vs.(1.09±0.59)years old] and[(8.6±1.8)kg vs.(9.5±2.1)kg], respectively. Patients in group Ⅱ had a lower SPO 2(0.75±0.09 vs. 0.85±0.09) and was more prone to appear hypoxic spell(30.2% vs. 19.7%) than patients in group Ⅰ. Patients in group Ⅱ had a more poor development in pulmonary artery. The ratio of McGoon, NAKATA index and Z score of pulmonary annulus in group Ⅱ were significantly less than that in group Ⅰ[1.4±0.3 vs. 1.9±0.5, (124±43)mm 2/m 2 vs.(222±88)mm 2/m 2 and-4.3±2.6 vs.-2.3±2.1], respectively. There was no difference of extubation time bewteen two groups. SPO 2 rose significantly from 0.75 to 0.83 after SPS. And after SPS, the diameter of main pulmonary artery, the ratio of McGoon, NAKATA index and Z score of pulmonary annulus increased significantly from 6.4 mm, 1.42, 126.4 mm 2/m 2 and -4.3 to 9.2 mm, 1.83, 212.6 mm 2/m 2 and -2.4, respectively. Moreover, LVEDD also increased significantly from 21.2 mm to 24.5 mm after SPS. Conclusion:SPS is safe and effective for little infants with anoxic spell and hypoplasia pulmonary arteries. SPS can improve oxygenation significantly and delay the age for radical surgery. SPS also promote the growth of the pulmonary arteries, pulmonary valve annulus and left ventricular, and it facilitates the preservation of pulmonary valve annulus and reduce the rate of transannular patching in a portion of infants.

8.
Article in Chinese | WPRIM | ID: wpr-911298

ABSTRACT

Objective:To identify the risk factors for early neurological complications after revascularization in adult patients with moyamoya disease.Methods:The medical records of patients of both sexes with moyamoya disease, aged 18-65 yr, of American Society of Anesthesiologists physical status Ⅱ or Ⅲ, who underwent revascularization in our hospital from January 2017 to June 2019, were retrospectively collected.According to the occurrence of early postoperative neurological complications, patients were divided into early postoperative neurological complication group and non-early postoperative neurological complication group.The factors such as patient′s age, gender, preoperative clinical symptoms, previous history of hypertension, history of diabetes, history of coronary heart disease, American Society of Anesthesiologists physical status, methods of anesthesia, type of operation, anesthesia time, time for start of operation, operation time, intraoperative urine volume, times of intraoperative vasoactive drugs used, and time of the post-anaesthesia observation room (PACU) stay were collected.Logistic regression analysis was used to identify the risk factors for postoperative early neurological complications.Results:A total of 510 adult patients with moyamoya disease underwent revascularization were enrolled in this study, and the incidence of early postoperative neurological complications was 9.0%.The results of logistic regression analysis showed that preoperative ischemia, intraoperative use of vasoactive drugs more than 3 times and PACU stay time>90 min were risk factors for postoperative neurological complications ( P<0.05). Conclusion:Preoperative ischemia, intraoperative use of vasoactive drugs >3 times and PACU stay time>90 min are risk factors for early neurological complications after revascularization in the patients with moyamoya disease.

9.
Article in Chinese | WPRIM | ID: wpr-910393

ABSTRACT

Objective:To study the feasibility that using size-specific dose estimation (SSDE) to estimate organ dose and effective dose in coronary CT angiography (CTA).Methods:Totally 421 consecutive patients with coronary artery CTA were included and retrospectively analized. All patients were scanned using the 3rd generation dual-source Force CT with prospectively ECG gated axis scan mode. The size specific dose estimation(SSDE) for each patient was conducted by calculate water equivalent diameters with Radimetrics. The organ doses of heart, lung, liver and breast, were estimated with Monte Carlo method. Patient-specific effective dose was calculated as a weighted sum of simulated organ doses with the coefficients from ICRP 103. Linear correlation analysis was used to validate the relationship between SSDE and organ doses as well as effective dose, and to derive coefficients for patient specific dose estimation. The mean error rate was used to evaluate estimation accuracy.Results:The CTDI vol, SSDE and effective dose were (16.8±8.7)mGy, (20.8±8.8)mGy and (4.4±2.9)mSv, respectively. The linear fitting formula for estimating organ dose based on SSDE were: Y=1.2 X-6.4 ( R2=0.91, P<0.05, mean error 0.1%) for heart, Y=1.4 X-7.4 ( R2=0.91, P<0.05, mean error 7.9%) for breast, Y=0.89 X-4.6 ( R2=0.86, P<0.05, mean error 8.3%) for lung, and Y=0.36 X-1.8 ( R2=0.64, P<0.05, mean error -17.9%) for liver. The linear fitting formula for estimating the individual effective dose based on SSDE were: Y=0.21 X-1.2 ( R2=0.92, P<0.05, mean error 0.2%) for men, Y=0.39 X-2.2 ( R2=0.93, P<0.05, mean error 1.7%) for women. Conclusions:In coronary artery CTA, the absorbed dose of the organs and patient specific effective dose could be estimated with SSDE and the corresponding conversion coefficients, which will help to achieve personalized assessment and precise management of patient radiation dose and risk in clinical practice.

10.
Article in Chinese | WPRIM | ID: wpr-910351

ABSTRACT

Objective:To investigate the value of the size-specific dose estimate (SSDE) on dose estimations of children's head CT scans.Methods:A retrospective study was conducted on plain head CT scans of 252 patients with the 64-row detector CT device of Discovery 750HD in the Union Hospital, Tongji Medical College, Huazhong University of Science and Technology from January to September in 2019. The volume CT dose index (CTDI vol)values were recorded. The head circumference (HC), area, and CT value were measured using a self-developed computer program, and the water equivalent diameter (WED), size-specific conversion factors ( f293 and f220), and absorbed dose (SSDE 293 and SSDE 220) were calculated according to the AAPM reports 293 and 220.The patients were divided into three groups by the quartering meth odaccording to their HC(<47.8 cm, 47.8-52.7 cm, >52.7 cm)and four groups based on their ages(0-2, 3-6, 7-10 and 11-14 years old). The difference between parameters ( f220 and f293, SSDE 220 and SSDE 293、SSDE 293 and CTDI vol) were compared for different groups, and the correlation of HC with f293 and SSDE 293 was analyzed. Results:There was an overestimation of f220 by 11.11% ( t=252.61, P<0.05) compared with f293. SSDE 220 was overestimated by 10.31% ( t=228.21, P<0.05) compared with SSDE 293, and SSDE 293 was underestimated by 9.60% ( t=-31.34, P<0.05)compared with CTDI vol. For the three HC groups, SSDE 220 was overestimated by 8.54%, 10.37%, and 11.57% ( t=73.73, 438.58, 275.52, P<0.05)compared with SSDE 293, and SSDE 293 was underestimated by 1.30%, 9.79%, and 14.61% ( t=-1.91, -60.95, -47.64, P<0.05)compared with CTDI vol. For the four age groups SSDE 220 was overestimated by 8.45%, 10.00%, 10.57%, and 11.36% ( t=63.58, 232.29, 247.84, 302.95, P< 0.05)compared with SSDE 293, and SSDE 293 was underestimated by 1.49%, 8.27%, 10.63%, and 13.78% ( t=-1.83, -28.27, -37.30, -49.80, P< 0.05)compared with CTDI vol. Furthermore, HC was highly correlated with f293 and SSDE 293 ( r2=0.88 and 0.76, respectively, P< 0.05). Conclusions:The radiation dose in children′s head CT scanning can be more accurately estimated according to the AAPM Report 293, while it can be overestimated by CTDI vol. Meanwhile, the CT radiation dose can be patently overestimated with the AAPM Report 220 compared with Report 293.HC is closely correlated with f293 and SSDE 293 and it can be used to estimatee more accurately for SSDE and the radiation dose received by children during head CT scanning.

11.
Article in Chinese | WPRIM | ID: wpr-908917

ABSTRACT

Objective:To explore the implementation method of case teaching in pharmacology teaching according to the standards of golden course.Methods:The students from Batch 2017 clinical medical students in our university were selected as the research objects, and randomized into the control group ( n=121) and the case teaching group ( n=171). The control group used online learning and traditional lecture, and the case teaching group adopted the online teaching and case teaching methods. And the teaching effect was evaluated by questionnaire survey and final performance analysis. Results:Compared with control group, the case teaching group had better final performance ( P<0.01). The questionnaire survey showed that the case teaching improved the learning interest of students and also enhanced their application ability of the knowledge ( P<0.01 or P<0.05). Conclusion:Case teaching has achieved good teaching effect in pharmacology teaching.

12.
Article in Chinese | WPRIM | ID: wpr-906392

ABSTRACT

Objective:To explore the effect of Gandou Fumu decoction (GDFMD) on the oxidative damage of HepG2 cells induced by CuCl<sub>2 </sub>based on the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling pathway. Method:CuCl<sub>2</sub> (200 μmol·L<sup>-1</sup>) was used to induce a copper-loaded HepG2 cell model. HepG2 cells were divided into a blank group (HepG2 cells + blank rat serum), a model group (HepG2 cells + CuCl<sub>2</sub> + normal rat serum), a GDFMD group (HepG2 cells + CuCl<sub>2</sub> + GDFMD-medicated rat serum), an inhibitor group (HepG2 cells + NVP-BEZ235 + normal rat serum), and a GDFMD + NVP-BEZ235 group (HepG2 cells + NVP-BEZ235 + GDFMD-medicated rat serum). ELISA method was used to determine superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, and malondialdehyde (MDA) content. The expression of microtubule-associated protein 1 light chain 3 (LC3) was detected by immunofluorescence. Phospho-PI3K/PI3K (p-PI3K/PI3K), p-Akt/Akt, p-mTOR/mTOR, Beclin-1, LC3Ⅱ/LC3Ⅰ, and p62/Actin were determined by Western blot. PI3K, Akt, mTOR, Beclin-1, LC3Ⅰ, LC3Ⅱ, p62 mRNA expression was measured by real-time polymerase chain reaction (PCR). Result:Compared with the blank group, the model group displayed decreased activities of SOD and GSH-Px and increased content of MDA (<italic>P</italic><0.01). Compared with the model group, the GDFMD group showed elevated activities of SOD and GSH-Px and reduced content of MDA (<italic>P</italic><0.05, <italic>P</italic><0.01), while the inhibitor group exhibited weakened GSH-Px activity and up-regulated content of MDA (<italic>P</italic><0.05). Compared with the blank group, the model group showed diminished expression of p-PI3K/PI3K, p-Akt/Akt, p-mTOR/mTOR, and p62, and increased expression of Beclin-1 and LC3Ⅱ/LC3Ⅰ (<italic>P</italic><0.01). The expression of p-PI3K/PI3K, p-Akt/Akt, p-mTOR/mTOR, and p62 was elevated, and the expression of Beclin-1 and LC3Ⅱ/LC3Ⅰ declined in the GDFMD group (<italic>P</italic><0.05,<italic> P<</italic>0.01), while the p-PI3K/PI3K and p-mTOR/mTOR expression was down-regulated and the Beclin-1 and LC3Ⅱ/LC3 I expression was increased in the inhibitor group (<italic>P</italic><0.05, <italic>P<</italic>0.01) as compared with those in the model group. Compared with the GDFMD group, the GDFMD + NVP-BEZ235 group showed down-regulated expression of p-Akt/Akt and p-mTOR/mTOR and up-regulated expression of Beclin-1 and LC3Ⅱ/LC3Ⅰ(<italic>P</italic><0.05, <italic>P</italic><0.01). The expression of LC3Ⅱ protein in the model group was increased (<italic>P</italic><0.01) as compared with that in the blank group. The expression of LC3Ⅱ protein was lower in the GDFMD group than in the model group, and higher in the GDFMD + NVP-BEZ235 group than in the GDFMD group. No significant difference in the expression of PI3K, Akt, and mTOR mRNA was observed among the groups. Compared with the blank group, the model group displayed lowered expression of p62 mRNA, and elevated expression of Beclin-1, LC3Ⅰ, and LC3Ⅱ mRNA (<italic>P</italic><0.01). Compared with the model group, the GDFMD group exhibited increased expression of p62 mRNA, and declining expression of Beclin-1, LC3Ⅰ, and LC3Ⅱ mRNA (<italic>P</italic><0.01), while the inhibitor group showed increased expression of Beclin-1 mRNA (<italic>P</italic><0.05). The expression of Beclin-1 and LC3Ⅱ mRNA in the GDFMD + NVP-BEZ235 group was elevated (<italic>P</italic><0.01) as compared with that in the GDFMD group. Conclusion:GDFMD may inhibit the excessive autophagy and alleviate the oxidative damage of HepG2 cells induced by CuCl<sub>2</sub>, with the underlying mechanism related to the activation of PI3K/Akt/mTOR signalling pathway.

13.
Article in Chinese | WPRIM | ID: wpr-906320

ABSTRACT

Perilla frutescens is a traditional medicinal and edible plant widely distributed in China and enjoys an extensive usage. P. frutescens contains multiple essential oils, which are composed of monoterpenes, sesquiterpenes, and their oxygen-containing derivatives. Compared with other parts of P. frutescens, Perillae Folium produce more oils, with volatile oils as the main constituents. There are many active substances in the volatile oils from Perillae Folium, mainly including perillaldehyde, perillaketone, perillaalcohol, D-limonene, β-caryophylene, etc. Such factors as germplasm, growth environment, extraction method, cultivation time, and harvest period all can trigger changes in volatile oil constituents and content from Perillae Folium. The volatile oils from Perillae Folium have diverse pharmacological effects like anti-oxidation, anti-bacteria, anti-inflammation, vasodilation, anti-tumor, and anti-depression, implying its high clinical application value. However, the chemical constituents in volatile oils from Perillae Folium are complex and unstable and their pharmacological activities are affected by many factors, so the safety and effectiveness of clinical medication fail to be guaranteed, which may has impeded the rational and effective use of these volatile oils. Many scholars in China and abroad have conducted a lot of research on the volatile oils from Perillae Folium, but there is currently no systematic and comprehensive research report on the chemical constituents of volatile oils from Perillae Folium and their pharmacological effects. This paper reviewed the relevant domestic and foreign literature, analyzed the development status of volatile oils from Perillae Folium, and summarized their extraction process, chemical constituents, and pharmacological actions, aiming to provide a reference for their further development, clinical application, and risk assessment.

14.
Article in Chinese | WPRIM | ID: wpr-906283

ABSTRACT

Objective:Based on database mining, the high-frequency compatibility of Caryophylli Fols as the core in formulas for treating diarrhea was analyzed, and the network pharmacology was used to elucidate the mechanism of the core drug group containing Caryophylli Fols in the treatment of diarrhea. Method:The online database of traditional Chinese medicine (TCM) was intelligently crawled by Python 3.8.1 programming, and the compatibility rules of Caryophylli Fols were analyzed, and the TCM with support≥0.30, confidence≥0.90 and lift≥1.00 was set as the core drug group of Caryophylli Fols. The components were searched and screened by TCM Systems Pharmacology Database and Analysis Platform (TCMSP), and the disease targets were collected in Therapeutic Target Datebase (TTD), GeneCards and DisGeNET database with "Diarrhea" as the key word. The network diagram of "TCM-ingredients-potential targets" was constructed by Cytoscape 3.7.1 software, and the network of protein-protein interaction (PPI) was constructed by STRING 11.0. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of potential targets was analyzed by R language, and the components of the core drug group were preliminarily verified and evaluated by Discovery Studio Client 2016 software. Result:A total of 155 formulas containing Caryophylli Fols for treating diarrhea were screened, involving 54 TCMs. The analysis of association rules showed that Caryophylli Fols was strongly associated with Myristicae Semen, Glycyrrhizae Radix et Rhizoma, Aucklandiae Radix and Atractylodis Macrocephalae Rhizoma in the treatment of diarrhea. The core drug group composed of these five TCMs involved 119 kinds of TCM ingredients and 114 potential targets, of which 104 potential targets were distributed in the nervous system, and the key targets were tumor protein p53 (TP53), transcription factor activator protein-1 (JUN), mitogen-activated protein kinase 8 (MAPK8), interleukin-6 (IL-6), 90 kDa heat shock protein <italic>α</italic>A1 (HSP90AA1) and so on. GO enrichment analysis mainly involved biological processes such as the regulation of neurotransmitter levels, blood circulation, hormone-mediated signal pathway and regulation of chemical synaptic transmission. IL-17 signal pathway, helper T cell 17 (Th17) cell differentiation, epidermal growth factor receptor and salmonella infection in KEGG pathways were closely related to the treatment of diarrhea. Molecular docking showed that the key target protein had high affinity with quercetin, kaempferol and <italic>β</italic>-sitosterol. Conclusion:The multi-components, multi-targets and multi-pathways involved in the core drug group of Caryophylli Fols are closely related to inflammation and nervous system, so it is speculated that it may treat diarrhea by repairing intestinal shielding integrity and regulating the levels of neurotransmitters.

15.
Article in Chinese | WPRIM | ID: wpr-906180

ABSTRACT

Objective:To observe the clinical efficacy of Didangtang combined with gemcitabine chemotherapy in postoperative patients with non-muscle invasive bladder ctraditional chinese medicineancer (NMIBC). Method:A total of 100 patients with NMIBC in Jiangsu Provincial Second Chinese Medicine Hospital and Jiangsu Province Hospital of Chinese Medicine from January 2015 to September 2020 were randomly divided into control group and treatment group. 50 cases in the treatment group received Didangtang combined with intravesical infusion of gemcitabine, and 50 cases in the control group received placebo combined with intravesical infusion of gemcitabine. Before and after the treatment cycle, the blood biochemistry, immune cell subsets(CD3<sup>+</sup>,CD4<sup>+</sup> and CD4<sup>+</sup>/CD8<sup>+</sup>) were detected to assess the safety and immune function changes in treatment group. Quality of life scale for patients with bladder cancer prepared by European Organization for the Research and Treatment of Cancer (EORTC QLQ-C30) and Specificity scale for patients with superficial bladder cancer (EORTC QLQ-BLS24) were scored to assess the changes in physical, role, emotional and social functioning of the patients, symptomatic conditions such as fatigue, pain, nausea and vomiting, shortness of breath, insomnia, loss of appetite, diarrhea and constipation, as well as bladder cancer-specific symptoms such as treatment-generated problems, future concerns, bowel symptoms and urinary tract symptoms. Result:There were no differences in terms of age, sex, disease duration and tumor states. During the treatment period, both groups had no recurrence or adverse events. The traditional Chinese medicine(TCM) efficacy in the treatment group was significantly better than that in the control group(<italic>χ<sup>2</sup></italic><sup> </sup>= 7.23,<italic>P </italic>= 0.02). After treatment, the number of CD3<sup>+</sup>,CD4<sup>+</sup> ,CD8<sup>+</sup> cells and the CD4<sup>+</sup>/CD8<sup>+</sup> ratio in the treatment group were significantly higher than those in control group(<italic>P</italic><0.05). Both groups showed significant improvements in the domains of role functioning, social functioning, and general health after treatment (<italic>P</italic><0.05). In addition, fatigue, diarrhea, constipation, intestinal symptoms, and urinary tract symptoms in the treatment group were significantly better than those in the control group after treatment (<italic>P</italic><0.05). Conclusion:Didangtang can reduce adverse clinical symptoms during postoperative gemcitabine infusion chemotherapy in patients with NMIBC, reduce urethral and intestinal discomfort reactions, improve immune function and enhance quality of life for patients.

16.
Article in Chinese | WPRIM | ID: wpr-906028

ABSTRACT

Objective:To study the effects of different processing methods on the anti-gouty arthritis and cardiotoxicity of Aconiti Radix, and to explore the possible attenuation and synergism mechanism of these different processing methods. Method:The swelling degree of knee joint, levels of inflammatory factors [interleukin (IL) -1<italic>β</italic>, IL-18, tumor necrosis factor (TNF)-<italic>α</italic>] and the activities of liver energy metabolism-related enzymes [Ca<sup>2+</sup>-Mg<sup>2+</sup>-adenosine triphosphatase (ATPase), Na<sup>+</sup>-K<sup>+</sup>-ATPase, succinate dehydrogenase (SDH)] in rats with gouty arthritis were used as indicators to evaluate the effects of pharmacopoeia steaming Aconiti Radix, pharmacopoeia boiling Aconiti Radix, Jianchang faction processed Aconiti Radix, Zhang faction processed Aconiti Radix and raw Aconiti Radix. The activity of creatine kinase (CK), lactate dehydrogenase (LDH) and the content of B-type natriuretic peptide (BNP) were used as indexes to evaluate the cardiotoxicity of Aconiti Radix and its different processed products. Result:In the anti-gouty arthritis test, compared with the blank group, the knee joint of the model group was significantly swollen (<italic>P</italic><0.01), the levels of IL-1<italic>β</italic>, IL-18, and TNF-<italic>α</italic> in serum were significantly increased (<italic>P</italic><0.01), and the Ca<sup>2+</sup>-Mg<sup>2+</sup>-ATPase activity was significantly decreased (<italic>P</italic><0.01). Compared with the model group, raw Aconiti Radix and the four processed products could reduce knee joint swelling and decrease IL-1<italic>β</italic>, IL-18, and TNF-<italic>α</italic> levels in serum of rats. The activity of Ca<sup>2+</sup>-Mg<sup>2+</sup>-ATPase in the liver of rats from the pharmacopoeia steaming Aconiti Radix group was significantly higher than that in the model group (<italic>P</italic><0.01), and there was no statistical difference in other groups. In the cardiotoxicity test, compared with the blank group, the activities of CK and LDH were significantly increased and the level of BNP was significantly increased in the raw Aconiti Radix group and the pharmacopoeia steaming/boiling Aconiti Radix groups (<italic>P</italic><0.01). In terms of LDH activity and BNP content, the Zhang faction and Jianchang faction processed Aconiti Radix groups were significantly lower than those in the raw Aconiti Radix group (<italic>P</italic><0.01). In the CK activity, the Zhang faction processed Aconiti Radix group was significantly lower than that in the raw Aconiti Radix group (<italic>P</italic><0.01). Conclusion:Raw Aconiti Radix and the four processed products have certain anti-inflammatory effects, but there are some differences among different indicators. There are significant differences in cardiotoxicity between the raw products and processed products of Aconiti Radix, and the cardiotoxicity of Jianchang faction and Zhang faction processed products was the weakest.

17.
Article in Chinese | WPRIM | ID: wpr-906008

ABSTRACT

Volatile oil is widely distributed in Chinese medicinal materials with complex chemical components. The main components are terpenes, aromatics, aliphatics, and nitrogen and sulfur containing. It has a variety of pharmacological activities. Such as antibacterial, anti-inflammatory, analgesic, anti-tumor, anti-oxidant, anti-aging and so on. It is widely used in medical and health care, agricultural efficiency enhancement, and daily products. In recent years, there have been a large number of studies on the pain relief of traditional Chinese medicine (TCM) essential oils, but there is no systematic generalization. The author found that the mechanism of TCM essential oils to exert analgesic effects mainly includes regulation of the central nervous system, anti-inflammatory and analgesic, antispasmodic and analgesic effects by consulting Chinese and foreign literatures in recent years, but the exact mechanism needs to be further verified. This article reviews the research progress of TCM essential oil pain relief from the aspects of pain classification, generation, analgesic mechanism and combination of other technologies, in order to provide reference for related research in the future.

18.
Article in Chinese | WPRIM | ID: wpr-905918

ABSTRACT

Cerebral ischemia/reperfusion injury (CIRI) is a common feature and the main pathophysiological mechanism of ischemic stroke(IS), which is caused by a blood reperfusion injury in ischemic brain tissues. It can aggravate brain tissue injury and cause irreversible brain damage, seriously affecting the quality of life or even the life of patients. Hence, we must find out the exact mechanism as well as the effective therapeutic drugs and targets for CIRI. The Chinese medicine effective in Xingnao (restoring consciousness) and Kaiqiao (opening orifices) has been widely used in the treatment of CIRI and serves as a classic therapy for IS. In recent years, scholars have conducted extensive and in-depth studies on the mechanism and therapeutic targets of Chinese medicine in Xingnao and Kaiqiao. They found that those drugs could interfere with a series of changes after IS and achieve the remarkable curative effect. This study summarized the effect and mechanism of Chinese medicine in Xingnao and Kaiqiao in the treatment of CIRI, including reducing the inflammatory response and oxidative stress, alleviating brain edema and the toxicity of excitatory amino acids, reducing cell apoptosis, promoting angiogenesis and neurovascular remodeling, and improving blood-brain barrier injury. It is expected to provide references to clarify the mechanism and important targets of those drugs in resisting CIRI and ideas for the in-depth investigation and application of brain protection of Chinese medicine in Xingnao and Kaiqiao.

19.
Article in Chinese | WPRIM | ID: wpr-905850

ABSTRACT

Succus Bambusae, regarded as the holy medicine for the treatment of phlegm by traditional Chinese medicine experts, has the functions of clearing heat and resolving phlegm, resuscitating and relieving convulsions. In clinical practice, it is mainly used to treat phlegm-heat cough, phlegm yellow and shortness of breath, stroke with exuberant phlegm and other syndromes. The research of its processing procedure and quality standard is the key issue to realize the process modernization and quality standardization of this traditional medicine resource. On the basis of literature research, this paper conducted the herbal textual research on Succus Bambusae, sorted out its medicinal history, and compared the advantages and disadvantages of its traditional and modern preparation procedure. Based on the historical records, it is pointed out that there are still many problems in the traditional and modern processing procedure of Succus Bambusae, such as low-yield processing procedure and extensive production technology that cannot meet the requirements of large-scale green manufacturing. At present, there are a lot of literature on the comparative study of the origin, variety and processing procedure of Succus Bambusae, but most of them are limited to the chemical components, and lack of in-depth study on its pharmacodynamic substance basis and mechanism. Meanwhile, the pharmacological effects of Succus Bambusae are mostly limited to antitussive and expectorant, and few other pharmacological effects have been reported. Based on the above analysis, it is suggested that the follow-up research work can be carried out from these five aspects:①Clarifying the original source, enriching and perfecting the medicinal resources. ②Standardizing the processing procedure, establishing relevant quality standards. ③Clarifying the pharmacological mechanism, strengthening the basic research on the pharmacological effect and clinical application. ④Inheriting ancient processing, researching and developing special preparation equipment and standardizing production operation rules. ⑤Comprehensive utilization of the by-products in preparation process.

20.
Article in Chinese | WPRIM | ID: wpr-905842

ABSTRACT

Objective:To establish a high performance liquid chromatography (HPLC) fingerprint of the substance benchmark of Xiao Chengqitang and evaluate its quality with chemical pattern recognition method. Method:Diamonsil C<sub>18</sub> column (4.6 mm×150 mm, 5 μm) was used, mobile phase was consisted of methanol (A)-0.1% phosphoric acid solution (B) for gradient elution (0-60 min, 20%-90%A; 60-70 min, 90%-100%A), the flow rate was 1 mL·min<sup>-1</sup>, the column temperature was 25 ℃, and the detection wavelength was 254 nm. The similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine (2012 edition) was used to evaluate the similarity of HPLC fingerprint of 15 batches of substance benchmark of Xiao Chengqitang, and the chromatographic data were analyzed by cluster analysis, principal component analysis and orthogonal partial least squares-discriminant analysis, in order to evaluate the quality difference between different batches of substance benchmarks of Xiao Chengqitang and find out the main chemical components that caused the quality difference. Result:The HPLC fingerprint of Xiao Chengqitang substance benchmarks was established, 31 common peaks were identified, and 18 components were identified by comparing with the reference substances. The similarities of 15 batches of HPLC fingerprint of Xiao Chengqitang substance benchmarks were >0.92. The samples could be divided into two categories by three chemical pattern recognition methods. Nine main components leading to the quality discrepancy of samples between batches were screened out, including rhein, chrysophanol-8-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucoside, aloe-emodin-8-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucoside, sennoside A, chrysophanol-1-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucoside, rhein-8-<italic>O</italic>-glucoside and others. Conclusion:The established fingerprint analysis method is accurate, stable and reproducible, which basically reflects the overall chemical composition characteristics of Xiao Chengqitang, and can be used for the quality control of Xiao Chengqitang preparations.

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