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1.
Article in Chinese | WPRIM | ID: wpr-907617

ABSTRACT

Objective:To develop the Traditional Chinese Medicine (TCM) symptom scores of qi depression and phlegm obstruction patternfor dyslipidemia patients, in order to optimize the TCM pattern evaluation method.Methods:According to Delphi method, the two roundsquestionnaires were distributed to experts through face-to-face interviews or emails, survey were recorded and analyzed via Excel 2016 and SPSS 17.0.Results:The experts are members of the Standing Committee of the Cardiovascular Diseases Branch of the China Association of Chinese Medicine, and the number of experts in the two rounds was 30 and 33, respectively. Active coefficients of both rounds were 100%. The Cronbach’s Alpha of consultations in the first and second rounds were 0.896 and 0.885, respectively. There were 36 symptom items in the initial scales of TCM symptom scores. In the first round, 17 items were eliminated, and in the second round, 1 item was added, 5 items were eliminated. The 15 items scales includededobesity, dizziness, head weight such as wrapping, head faintness, mouth viscosity, phlegm, chest depression, chest and flank distension, abdominal distension, heavy numbness, fatigue and fatigue, body weight, depression, stagnant stool and sighing frequently. The items for symptom rating scale were suitable.Conclusion:The TCM symptom scores of qi depression and phlegm obstruction pattern can be used to evaluate the effect of TCM treatment for the patients with dyslipidemia and TCM pattern of qi depression with phlegm obstruction, and the studyprovides important guidance for TCM in the diagnosis and treatment of dyslipidemia. In addition, the enthusiasm and authority of experts, as well as its concentration, reliability and coordination, are all well considered in this study, and the results of this consultation are desirable.

2.
Article in Chinese | WPRIM | ID: wpr-907345

ABSTRACT

Stroke has become one of the main causes of death in China, and ischemic stroke is the most common type. At the same time, the prevalence of chronic kidney disease (CKD) is also increasing, and there are many similar risk factors with ischemic stroke. More and more studies have shown that CKD increases the incidence, severity and risk of poor outcome of ischemic stroke, but the specific mechanism of its interaction is not completely clear. For ischemic stroke patients with CKD, the most effective treatment and prevention measures are lack of sufficient evidence. This article reviews the relationship between CKD and ischemic stroke, as well as the treatment and prevention.

3.
Article in English | WPRIM | ID: wpr-888688

ABSTRACT

Although microRNA-155 (miR-155) is considered a pro-inflammatory mediator, cumulative evidence indicates that it also has anti-inflammatory effects in macrophages and dendritic cells. In this study, we identified the dramatic expression changes of more than half of potential miR-155-targeted genes upon lipopolysaccharide (LPS) stimulation; 223 genes were down-regulated and 85 genes were up-regulated, including suppressor of cytokine signaling 1 (

4.
Article in English | WPRIM | ID: wpr-888509

ABSTRACT

Proteasome is the eukaryotic organelle responsible for degradation of short-lived proteins and involved in maintaining cellular protein homeostasis. It has been reported that during the occurrence and development of hepatocellular carcinoma (HCC), the regulatory particle subunits of proteasome regulate a series of tumor-related proteins, and proliferation, survival-associated signaling molecules, including PTEN gene, P53, Bcl-2, Bcl-2 interacting mediator of cell death (Bim), cyclin-dependent kinase 4(CDK4), transforming growth factor β receptor (TGFBR), E2F1, growth factor receptor-bound protein 2 (GRB2) . Meanwhile, these subunits regulate some tumor-associated pathway protein, such as signal transducer and activator of transcription 3 (STAT3) and protein kinase B (AKT), inducing their malfunction to promote the occurrence, proliferation, invasion and metastasis of HCC. The core particle subunits are more to perform the degradation of HCC-related proteins, so inhibitors targeting the core particle show a good anti-tumor effect. This review summarizes the current research progress on the regulation and mechanism of proteasome subunits in promoting the occurrence and development .


Subject(s)
Carcinoma, Hepatocellular , Cell Line, Tumor , Cell Proliferation , Humans , Liver Neoplasms , Proteasome Endopeptidase Complex/metabolism , STAT3 Transcription Factor/metabolism , Signal Transduction
5.
Chinese Journal of Neurology ; (12): 392-398, 2021.
Article in Chinese | WPRIM | ID: wpr-885434

ABSTRACT

Primary angiitis of the central nervous system is relatively rare. In recent years, research of the disease in neuroimaging, differential diagnosis, treatment and other aspects advanced greatly. This paper provided a systematic review of this disease entity in order to promote neurologists, neurosurgeons and radiologists′ experience in the diagnosis and treatment of the disease.

6.
Article in Chinese | WPRIM | ID: wpr-883584

ABSTRACT

Objective:To explore the feasibility and application effect of Mini-CEX in pediatric clinical practice.Methods:Residents who received standardized residency training in pediatrics department of Peking University Third Hospital from March 2016 to September 2017 were selected as research objects. The clinical ability of the residents were evaluated by application of the Mini-CEX structured form. Its effectiveness as a training guide and assessment method was also evaluated. SPSS 25.0 was used for t test. Results:The Mini-CEX scale was refined according to the characteristics of pediatrics. In this study, a total of 36 residents participated in the evaluation and 110 cases were completed by introducing Mini-CEX structured form in 7 examinations. Compared with 2016, the Mini-CEX scores of the residents in the 2017 showed different degrees of improvement in information gathering [(7.4±0.9) vs. (7.7±0.7)], clinical examination [(7.5±1.1) vs. (7.6±0.9)], clinical judgment [(7.5±0.9) vs. (7.6±0.9)], organizational effectiveness [(7.4±0.9) vs. (7.7±0.9)], and overall clinical competency [(7.5±0.9) vs. (7.7±0.9)], without significant differences ( P > 0.05). A total of 17 residents completed the assessments more than or equal to 3 times. Compared with 2016, the mean scores of the 17 individuals' Mini-CEX scales in 2017 were significantly improved in terms of information gathering, organizational effectiveness, and overall clinical competency. Conclusion:The results have shown that the Mini-CEX scale is simple and easy to operate, it maybe helpful to improve the clinical ability of pediatric residents, and it can be used as a method to evaluate the clinical ability of pediatric residents.

7.
Article in Chinese | WPRIM | ID: wpr-883515

ABSTRACT

Parkinson's disease(PD)is the second most common neurodegenerative disease in the world;however,it lacks effective and safe treatments.Ginkgo biloba dropping pill(GBDP),a unique Chinese G.biloba leaf extract preparation,exhibits antioxidant and neuroprotective effects and has a potential as an alternative therapy for PD.Thus,the aims of this study were to evaluate the effects of GBDP in in vitro and in vivo PD models and to compare the chemical constituents and pharmacological activities of GBDP and the G.biloba extract EGb 761.Using liquid chromatography tandem-mass spectrometry,46 GBDP constitu-ents were identified.Principal component analysis identified differences in the chemical profiles of GBDP and EGb 761.A quantitative analysis of 12 constituents showed that GBDP had higher levels of several flavonoids and terpene trilactones than EGb 761,whereas EGb 761 had higher levels of organic acids.Moreover,we found that GBDP prevented 6-hydroxydopamine-induced dopaminergic neuron loss in zebrafish and improved cognitive impairment and neuronal damage in methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mice.Although similar effects were observed after EGb 761 treatment,the neuroprotective effects were greater after GBDP treatment on several endpoints.In addition,in vitro results suggested that the Akt/GSK3β pathway may be involved in the neuroprotective effects of GBDP.These findings demonstrated that GBDP have potential neuroprotective effects in the treatment of PD.

8.
Article in Chinese | WPRIM | ID: wpr-883172

ABSTRACT

Objective:To investigate the application of umbilical vein catheterization in neonates.Methods:The patients who underwent umbilical vein catheterization from November 2007 to September 2019 in neonatal intensive care unit (NICU) at Peking University Third Hospital were selected consecutively.Clinical data were collected retrospectively to investigate the application time of umbilical vein catheterization in neonates, the incidence of catheter-related bloodstream infection, pathogenic bacteria and the causes of extubation.Results:A total of 835 newborns were enrolled, including 435 males (52.1%) and 400 females (47.9%). The average gestational age was (30.6±2.4) weeks.The median birth weight was 1 310(1 080, 1 520)g.The average indwelling time of umbilical vein catheterization was (4.850±1.893) days.Catheter related bloodstream infection occurred in ten (1.2%) neonates.The main pathogens were Staphylococcus epidermidis, Staphylococcus wallichi, Staphylococcus haemolyticus and Enterococcus faecium.A total of 770 (92.2%) newborns were extubated in a planned manner, and 65 (7.8%) were unplanned extubation.The top three causes of unplanned extubation were umbilical wheel swelling, clinical diagnosis of sepsis, catheter-related bloodstream infection and tube blockage.Conclusion:Umbilical vein catheterization is simple, high success rate, safe and can be used in NICU for a short time.If possible, abdominal B-ultrasound and echocardiography should be monitored and catheter related complications should be noted.

9.
Article in Chinese | WPRIM | ID: wpr-882978

ABSTRACT

Fetal omphalocele is a surgical disease with severe defect of fetal abdominal wall which requires emergency treatment after birth. The incidence rate is 2.1/10000, and the mortality rate is 52% to 67%. The improper handling of omphalocele can cause capsular rupture and intestinal exposure, leading to neonatal fluid loss, hypothermia, intestinal necrosis and other complications. It is the major cause of death in newborns. In this paper, we reviewed the characteristics, delivery mode, delivery time, umbilical cord care, and transferring of omphalocele newborns to provide reference for clinical nursing.

10.
Article in Chinese | WPRIM | ID: wpr-910439

ABSTRACT

Objective:To investigate the effect and mechanism of RNaseH-1 on the radiosensitivity of the osteosarcoma cells via the alternative lengthening of telomeres (ALT) mechanism to maintain the telomere length.Methods:ALT osteosarcoma cell U2OS and telomerase-positive osteosarcoma cell 143B over-expressing RNaseH-1 were constructed by lentiviral transfection. After cell transfection, cell proliferation and cell cycle were determined using CCK-8 assay and flow cytometry. The effect of RNaseH-1 on the radiosensitivity of osteosarcoma cells was examined by colony formation assay. DNA injury (γ-H 2AX foci) was assessed by immunofluorescent assay. The expression levels of related proteins were detected by Western blot. Results:The proliferation abilities of U2OS cells were significantly declined following the over-expression of RNaseH-1, and G 1 cell cycle arrest was noted (all P<0.05). Over-expression of RNaseH-1 in U2OS cells increased the phosphorylated levels of ATM and Chk 2, down-regulated the expression of homologous recombination (HR)-related proteins RAD51 and BRCA1significantly aggravated DNA damage and remarkably enhanced the radiosensitivity (all P<0.05). Over-expression of RNaseH-1 exerted no inhibitory effect upon the telomerase-positive 143B cells ( P>0.05). Conclusion:RNaseH-1 over-expression suppresses telomerase-negative osteosarcoma cells and enhances the radiosensitivity probably via the role of RNaseH-1 in inhibiting the homologous recombination repair and activating the ATM signaling pathway.

11.
Article in Chinese | WPRIM | ID: wpr-871083

ABSTRACT

Objective:To investigate the relationship of umbilical arterial blood pH with Apgar score and its perinatal influencing factors as well as effects on short-term neonatal outcomes.Methods:This study retrospectively collected the clinical data of 7 183 singleton newborns who were born at ≥35 gestational weeks with umbilical artery blood gas analysis in Peking University Third Hospital from January 2017 to February 2019. All cases were divided into two groups according to their Apgar scores at 1 min: low Apgar group (≤7 scores, n=42) and normal Apgar group (>7 scores, n=7 141). Moreover, according to the pH value of umbilical artery blood they were also allocated into acidosis group (pH<7.2, n=379 ) and normal pH group (pH≥7.2, n=6 804). Independent sample t test, Chi-square test, Fisher's exact test, Pearson's correlation analysis and multivariate logistic regression analysis were used to analyze the correlation between umbilical arterial blood pH and Apgar score, effects of perinatal complications on umbilical arterial blood pH, and influences of acidosis on early neonatal outcomes. Results:The umbilical arterial blood pH in the low Apgar group was significantly lower than that in the normal Apgar group (7.19±0.13 vs 7.32±0.07, t=-6.011, P<0.001). There was a positive correlation between the umbilical arterial blood pH and Apgar score at 1 min ( r=0.217, P<0.001). Fetal distress was an independent risk factor for low Apgar score ( OR=20.553, 95% CI: 4.380-96.443, P<0.001). Premature rupture of membranes was an independent risk factor for acidosis ( OR=1.316, 95% CI: 1.035-1.673, P=0.025). The incidence of low Apgar score [6.33% (24/379) vs 0.26% (18/6 804), χ2=217.075], respiratory distress [6.60% (25/379) vs 2.62% (178/6 804), χ2=21.205] and intracranial hemorrhage [1.06% (4/379) vs 0.04% (3/6 804), Fisher's exact test] was significantly higher in the acidosis group than in the normal pH group (all P<0.001). Conclusions:Neonates with lower umbilical arterial blood pH value should be closely monitored after birth as they are more likely to have respiratory distress and intracranial hemorrhage. Umbilical arterial blood pH value may be effective and is recommended in predicting neonatal early outcome.

12.
Article in Chinese | WPRIM | ID: wpr-869250

ABSTRACT

Objective:To investigate the effect of a family support nursing intervention on mood, negative psychology, quality of life, and illness perception among elderly patients with breast cancer after radical operation.Methods:A total of 94 patients with breast cancer who had under gone radical mastectomy during the period from August 2016 to May 2019 at the Seventh People′s Hospital of Zhengzhou were randomly divided into two groups: a regular care group (control group, 45 patients) and a family support group (49 patients). The regular care group was provided with routine follow-up care after discharge. The family support group was given a family support nursing intervention, along with routine follow-up care. Hamilton Anxiety Rating Scale (HAM-A), Hamilton Depression Rating Scale (HAM-D), quality of life, and illness perception scores were compared between the two groups before and after the intervention.Results:There were no statistically significant differences in HAM-A, HAM-D, quality of life (physical status, emotional status, functional status, breast cancer-specific module scores, and total score), and illness perception scores (cognitive, emotional, comprehension, and total scores) between the two groups before the intervention ( P>0.05). The HAM-A and the HAM-D scores in the family support group were lower than those in the regular care group after the intervention [(15.2±1.7) vs. (19.3±2.0) points, (18.0±1.7) vs. (23.1±2.5) points] ( t=8.061, 8.021; both P<0.001). Physical status, emotional status, functional status, breast cancer-specific module, and total scores in the family support group were higher than those in the regular care group [(21.3±2.5) vs. (16.4±2.1) points, (18.4±2.0) vs. (15.1±1.7) points, (22.0±2.6) vs. (15.1±1.5) points, (28.8±3.6) vs. (24.8±2.6) points, (113.3±15.5) vs. (101.4±12.5) points] ( t=10.126, 8.695, 15.508, 6.205, 4.091; all P<0.001). The cognitive, emotional, comprehension, and total scores in the family support group were lower than those in the regular care group [(29.4±3.0) vs. (34.9±3.7) points, (10.3±1.7) vs. (13.0±1.4) points, (4.1±0.5) vs. (7.4±0.8) points, (48.9±5.3) vs. (59.0±6.1) points] ( t= 8.063, 8.285, 24.456, 8.597; all P<0.001). Conclusion:Interventions that support the family can improve mood and quality of lifeand enhance illness perception among elderly patients with breast cancer after radical mastectomy.

13.
Article in Chinese | WPRIM | ID: wpr-810409

ABSTRACT

Objective@#To explore the acute effect of fine particulate matters (PM2.5), O3, NO2 on daily non-accidental mortality, cardiovascular disease mortality and respiratory mortality data in thirteen cities of Jiangsu province.@*Methods@#Daily average concentrations of non-accidental mortality, cardiovascular disease mortality, respiratory mortality data and environmental data were collected from January 1, 2015 to December 31, 2017 in thirteen cities of Jiangsu Province. Daily air quality, mortality and meteorology data were collected from the Information System of Air Pollution and Health Impact Monitoring of Chinese Center for Disease Control and Prevention. We used generalized additive model to evaluate the association between daily concentrations of air pollutants and mortality at single-city level and multi-city level, after adjusting the long-term and seasonal trend, as well as meteorological factors and the effect of "days and weeks" . A multivariate Meta-analysis with random effects was applied to estimate dose-response relationship between air pollutants and mortality.@*Results@#At multi-city level, per interquartile range increase of PM2.5, O3, NO2 was associated with an increase of 1.10% (95%CI: 0.66%, 1.54%), 0.59% (95%CI: 0.18%, 1.00%), 2.00% (95%CI: 1.29%, 2.72%) of daily non-accidental mortality respectively; 1.01% (95%CI: 0.63%, 1.38%), 0.66% (95%CI: 0.02%, 1.30%), 1.62% (95%CI: 1.00%, 2.23%) of daily cardiovascular mortality respectively; 1.09% (95%CI: 0.35%, 1.82%), 0.44% (95%CI: -0.29%, 1.16%), 2.75% (95%CI: 1.42%, 4.08%) of daily respiratory mortality respectively. The air pollutants effect varied across different cities. The strongest effect of PM2.5 was current day (excess risk (ER)=1.10%, 95%CI: 0.66%, 1.54%)), the strongest effect of O3 was 2-day lag (ER=1.82%, 95%CI: 0.69%, 2.97%) and the strongest effect of NO2 was 1-day lag (ER=2.09%, 95%CI: 1.34%, 2.83%) of daily non-accidental mortality respectively.@*Conclusion@#The increases of PM2.5 and NO2 concentration could result in the increases of daily non-accidental mortality, cardiovascular disease mortality and respiratory mortality. O3 could result in the increases of daily non-accidental mortality and cardiovascular disease mortality. The acute effects for non-accidental mortality from high to low were NO2, PM2.5 and O3, and the strongest effect of PM2.5 was current day. O3 and NO2 had lagged effects.

14.
Article in Chinese | WPRIM | ID: wpr-776766

ABSTRACT

OBJECTIVE@#To explore the genetic basis for a family with non-syndromic autosomal recessive deafness.@*METHODS@#The proband and her parents were subjected to physical and audiological examinations. With genomic DNA extracted from peripheral blood samples, next-generation sequencing was carried out using a panel for deafness genes. Suspected mutation was validated by Sanger sequencing and qPCR analysis of her parents.@*RESULTS@#The proband presented bilateral severe sensorineural hearing loss at three days after birth. Her auditory threshold was 110-120 dBnHL but with absence of vestibular and retinal symptoms. Her brother also had deafness but her parents were normal. No abnormality was found upon physical examination of her family members, while audiological examination showed no middle ear or retrocochlear diseases. Next-generation sequencing identified compound heterozygous mutations of the MYO7A gene, including a previously known c.462C>A (p. Cys154Ter) and a novel EX43_46 Del, which were respectively derived from her mother and father.@*CONCLUSION@#The compound heterozygous mutations of the MYO7A gene probably underlie the disease in this family. Our findings has enriched the mutation spectrum for non-syndromic autosomal recessive deafness 2.


Subject(s)
Female , Hearing Loss, Sensorineural , Genetics , High-Throughput Nucleotide Sequencing , Humans , Male , Mutation , Myosins , Genetics , Pedigree
15.
Article in Chinese | WPRIM | ID: wpr-796458

ABSTRACT

Objective@#To explore the genetic basis for a family with non-syndromic autosomal recessive deafness.@*Methods@#The proband and her parents were subjected to physical and audiological examinations. With genomic DNA extracted from peripheral blood samples, next-generation sequencing was carried out using a panel for deafness genes. Suspected mutation was validated by Sanger sequencing and qPCR analysis of her parents.@*Results@#The proband presented bilateral severe sensorineural hearing loss at three days after birth. Her auditory threshold was 110-120 dBnHL but with absence of vestibular and retinal symptoms. Her brother also had deafness but her parents were normal. No abnormality was found upon physical examination of her family members, while audiological examination showed no middle ear or retrocochlear diseases. Next-generation sequencing identified compound heterozygous mutations of the MYO7A gene, including a previously known c. 462C>A (p. Cys154Ter) and a novel EX43_46 Del, which were respectively derived from her mother and father.@*Conclusion@#The compound heterozygous mutations of the MYO7A gene probably underlie the disease in this family. Our findings has enriched the mutation spectrum for non-syndromic autosomal recessive deafness 2.

16.
Article in Chinese | WPRIM | ID: wpr-743962

ABSTRACT

Single-port and reduced-port laparoscopic radical gastrectomy as the innovative surgery for gastric cancer are gradually accepted nowadays,and more attentions are also paid to single-port and reduced-port laparoscopic radical gastrectomy due to its better cosmetic effect,less pain,lower incidence rate of surgical site infection and more advantages in enhanced recovery after surgery.However,in the early stage,the development of single-port and reduced-port laparoscopic radical gastrectomy were facing challenges and obstacles on account of limited surgical skills,the lack of special laparoscopic instruments,laparoscope and multi-port Trocar.In recent years,the dilemma and difficult situations were gradually resolved following by surgical techniques innovation,laparoscopic instruments and facilities improvement.It is believed that single-port and reduced-port laparoscopic radical gastrectomy will have a good prospect and breakthrough in the field of gastric cancer treatment in the future.

17.
Article in Chinese | WPRIM | ID: wpr-712546

ABSTRACT

Objective To study the problems encountered in the coding of cataract surgeries. Methods Medical record statistical system was used to retrieve 213 such patients discharged from a hospital between January and June 2016. ICD-9-CM-3 coding principle was called into play to analyze the form completion and coding of major surgeries, along with errors found in them. Results Study of the 213 medical records identified 12 errors of surgery form completion from the surgeons′medical record homepages, accounting for 5. 6% of all. With reference to ICD-9-CM-3, 188 errors were found from the surgical coding made by encoders, accounting for 88. 3% of all. 10 copies were missing with surgery coding, accounting for 4. 7%. 13 coding errors were found in major surgical choices, accounting for 6. 1%. Conclusions Failure of the encoders in their command of coding rules for cataract surgery tops the rest as the main reason for the error of surgical coding. Encoders should make effective use of the ICD-9-CM-3 manual, and master both clinical knowledge, professional skill and coding ability.

18.
Article in Chinese | WPRIM | ID: wpr-712518

ABSTRACT

Progress of the DRGs payment reform is raising higher demands on coders'competence at county-level hospitals. These coders at present are mostly transferred from nurses or medical technicians, who tend to be older in age and lower in education and academic title. To improve their competence, it is imperative to promote coders cultivation in terms of regular career needs, self-determination theory and performance management. Coder ranks deserve attention to build professional and outstanding coders.

19.
Chinese Journal of Neurology ; (12): 942-948, 2018.
Article in Chinese | WPRIM | ID: wpr-711056

ABSTRACT

Objective To report the clinical features, myopathological changes, and gene mutations in five Chinese patients with mitochondrial diseases caused by POLG gene mutations. Methods Clinical materials of five unrelated patients who were referred to Department of Neurology, Peking University First Hospital from April 2012 to January 2018, carrying POLG gene mutations, were retrospectively analyzed. Muscle/nerve biopsies and targeted second-generation gene sequencing were performed on the patients. Results Among the five patients, three were male and two were female. Two cases were dominant inheritance and three were sporadic or recessive inheritance. The ages of onset were from 15 to 40 years with disease course of one to 26 years. One of them showed atypical SANDO (sensory ataxic neuropathy, dysarthria, and ophthalmoparesis) syndrome accompanied by cardiac preexcitation syndrome. There were two cases with autosomal dominant and one case with recessive progressive external ophthalmoplegia plus syndrome. One case presented with cognitive delay and sensory neuropathy. The pathological changes of mitochondrial myopathy were observed in all four patients with muscle involvement. Sural nerve biopsy in the patient with cognitive delay and sensory ataxia revealed chronic axonal pathological changes. POLG gene mutations were found in all five patients by targeted next generation sequencing, including single heterozygous mutations in two dominant inherited patients (c. 914 G>A and c. 2864A>G, respectively), and compound heterozygous POLG gene mutations in the other three sporadic/recessive inherited patients (c. 2591 A>G/c. 1790 G>A, c. 924G>T/c. 3002delG and c. 1613A>T/c. 1612 G>T, respectively). There were six novel mutations not reported before, i.e., c.914G>A(p.S305N), c.924G>T(p.Q308H), c.1613A>T(p.E538V), c.1612G>T(p.E538*), c.1790 G>A(p.R597Q) and c.3002delG. Conclusions POLG gene mutations can lead to different clinical spectrums. Progressive external ophthalmoplegia, limb weakness and axonal sensory neuropathy are common presentations in this group of patients with POLG gene related mitochondrial neuromuscular diseases. Novel mutations found in this study expand the mutational spectrum of POLG gene.

20.
Article in Chinese | WPRIM | ID: wpr-710075

ABSTRACT

Objective To evaluate the injury of retinal microstructure using optical coherence tomography (OCT) and investigate the role of aquaporin 4 antibody (AQP4 Ab) in this injury process.Methods Forty patients with neuromyelitis optica spectrum disorders (NMOSD) were retrospectively studied,each of whom reported at least one episode of optic neuritis (ON),namely 59 ON eyes involved in all.All patients were divided into two subgroups based on AQP4 Ab tests including 25 patients (37 ON eyes) with AQP4 positive (Ab+/NMOSD group) and 15 patients (22 ON eyes) negative (Ab-/NMOSD group).In addition,10 healthy controls (20 eyes) matched for age and sex (HC group) were analyzed.Spectral domain optical coherence tomography (SD-OCT) was used to quantify peripapillary retinal nerve fiber layer (RNFL).Nonparametric test was used to compare differences between groups.Results Age distribution and gender ratio were comparable in three groups (P>0.05).Visual acuity in ON eyes of Ab+/NMOSD group was worse than that of Ab-/NMOSD group (P=0.02).There were no significant differences between Ab+/NMOSD and Ab-/NMOSD in aspects of disease duration (2.6 vs.1.9 year),ON episodes (2 vs.1),longitudinal extensive transverse myelitis (LETM) ratio (48.0% vs.66.7%),NMOSD specific intracranial lesions ratio (32.0% vs.53.3%),positive autoimmune antibody ratio (52.0% vs.20.0%) (P=0.13,0.08,0.25,0.18,0.06,respectively).The thickness of temporal,superior,nasal,inferior and average RNFL in ON eyes of both Ab+/ NMOSD and Ab-/NMOSD group were thinner than those in eyes of HC group (all P<0.05).The thickness of superior and inferior RNFL in ON eyes of Ab+/NMOSD were 61.0 μm and 62.0 μm,which was thinner than those of Ab-/NMOSD 94.5 μm and 97.0 μm (P=0.03 and 0.01,respectively).Conclusions RNFL reflects the injury of retinal microstructure in NMOSD patients.AQP4 Ab seropositivity is correlated to the severity of RNFL damage,implying the potential role of AQP4 Ab in this pathological process.

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