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1.
Article in Chinese | WPRIM | ID: wpr-880119

ABSTRACT

OBJECTIVE@#To detecte the carrying rate, the type and distribution of α-Thalassemia gene mutation in Honghe Prefecture, Yunnan Province, and analyze the differences in average erythrocyte volume (MCV), mean erythrocyte hemoglobin content (MCH) and hemoglobin among different types of α-Thalassemia.@*METHODS@#The DNA samples from small cell hypochromic carriers or anemia patients and women of childbearing age who underwent hematological screening in The First People's Hospital of Honghe State was from 2015 to 2019 were enrolled and analyzed, and the mutation types and frequency of alpha-thalassemia positive rate were diagnosed by PCR reverse dot blot or PCR fluorescence dissolution curve.@*RESULTS@#Among the 1 016 samples, 141(13.88%) of the patients were diagnosed as α-thalassemia. The α-thalassemia was subdivided into 3 types, silent (36.17%), minor (51.77%), and HbH disease (12.06%), and the MCV, MCH and HB levels were detected and showed a obvious decrease trend with significant difference (P < 0.05). The gene mutation types were 9 kinds, the deletion type gene was mainly --SEA (51.06%), followed by -α@*CONCLUSION@#Alpha-thalassemia in Honghe prefecture of Yunnan Province shows complex genetic diversity and significant genetic heterogeneity, and the mainly type of gene mutation is --SEA and --


Subject(s)
China , Female , Genotype , Heterozygote , Humans , Mutation , alpha-Thalassemia/genetics , beta-Thalassemia
2.
Article in Chinese | WPRIM | ID: wpr-923743

ABSTRACT

@#Objective To optimize the ways of extubation after tracheotomy.Methods From August, 2016 to April, 2018, 118 patients after tracheotomy for brain injury were divided into early extubation group (n=74) and conventional extubation group (n=44). The success rate and tolerance of extubation were compared, and the biochemical markers of blood were tested before and seven days after extubation. The early extubation group was divided into disposable extubation group (n=37) and occlusion tube-extubation group (n=37), and their success rates of extubation were compared.Results There was no significant difference in success rate between the early extubation group and the conventional extubation group (χ2=0.016, P>0.05). The tolerance was less in the early extubation group on the first day of extubation (χ2=4.909, P<0.05), and it was not different seven days and 15 days after extubation (χ2<1.995, P>0.05). The procalcitonin, hypersensitive C reactive protein and white blood cell count decreased (t>2.680, P<0.05), and hemoglobin and albumin increased seven days after extubation (t>11.620, P<0.001). There was no significant difference in the success rate between the disposable extubation group and the occlusion tube-extubation group (χ2<2.902, P>0.05).Conclusion Satisfactory cough and deglutition reflex are the core indexes of successful extubation, other indications may not influence on the success of extubation but on tolerance. Extubation may benefit to control the complications of tracheotomy. The disposable extubation is more recommended.

3.
China Pharmacy ; (12): 2750-2753, 2017.
Article in Chinese | WPRIM | ID: wpr-616332

ABSTRACT

OBJECTIVE:To investigate the influence of cephalosporins antibiotics on cost and length of hospital stay in pa-tients underwent gallbladder calculus resection,and to provide reference for drug use in clinic. METHODS:A total of 1866 patients underwent gallbladder calculus resection were collected from 9 third grade class A hospitals in Guangxi during 2013-2014. SPSS 22.0 software was adopted to analyze cost and length of hospital stay in patients underwent gallbladder calculus resection. RESULTS:Multi-factor analysis showed that the factors which significantly affected the cost and length of hospital stay were antibiotics,hospi-tals,age,surgery types and disease species (all P0.05). CONCLUSIONS:Cephalospo-rins antibiotics can significantly influence the cost and length of hospital stay in patients underwent gallbladder calculus resec-tion. Antibiotics selection canbe considered comprehensively ac-cording to local medical resources and the patients'demands.

4.
Article in Chinese | WPRIM | ID: wpr-853667

ABSTRACT

Objective: To explore the mechanism of the compatibility of Mongolian medicine Sendeng-4. Methods: Sendeng-4 was composed of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction with Gardeniae Fructus, Toosendan Fructus, and Chebulae Fructus with a mass ratio of 5:3:1:1. BEH C18 column (50 mm×2.1 mm, 1.7 μm) was used as the chromatographic column, water (0.1% formic acid)-methanol were used as mobile phase for the gradient elution, and the ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS), principle component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze the chemical constituent changes of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction. Results: The significant differences between the group of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and Sendeng-4 were observed in PCA and OPLS-DA model. There were significant differences among eight chemical compounds after compatibility (P<0.05). The relative contents of catechin, (-)-gallocatechin, dihydromyricetin, quercetin decreased, xanthocerasic acid, 3β-hydroxytirucalla-7,24-dien-21-oic acid, 3-oxotirucalla-7,24-dien-21-oic acid, and rutin were increased. Conclusion: Maybe the anti-inflammatory and anti-rheumatic activity of Sendeng-4 have the relationship with the content changes of some index component in Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium after compatibility.

5.
Article in Chinese | WPRIM | ID: wpr-327669

ABSTRACT

Since plague is an important natural focus zoonosis, the typing of natural plague foci becomes one of the elements in understanding the nature and developing related prevention program of the disease. Natural foci of plague are composed by four fundamental parts which include Eco-geographical landscape (natural plague foci), hosts, vectors and pathogens (Yersinia pestis) that comprehensively interact through the large temporal scale of evolution. Human activities have had great impact on the foci of natural plague. Based on the published serial research papers, we tried to integrate the knowledge of each factor in natural plague foci and focusing on theoretical aspects, so as to strengthen the prevention and surveillance programs of plague to be extrapolated to other zoonosis.


Subject(s)
Animals , Biological Evolution , China , Epidemiology , Disease Reservoirs , Geography , Insect Vectors , Plague , Epidemiology , Yersinia pestis , Genetics
6.
Chinese Journal of Epidemiology ; (12): 1144-1150, 2012.
Article in Chinese | WPRIM | ID: wpr-289564

ABSTRACT

Objective To group and characterize natural plague foci in China.Methods A novel two-class typing method as well as a three-indication nomenclature method were established to group and characterize the natural plague foci,on the basis of eco-geographical landscapes of plague foci,genetics of Yersinia pestis,zoology of rodent reservoirs and the entomology of flea vectors.Results A total of 12 distinct natural plague foci (including 19 subtypes) as well as their biological features were characterized.Conclusion Natural plague foci in China were grouped and characterized in this study.

7.
Chinese Journal of Epidemiology ; (12): 818-822, 2012.
Article in Chinese | WPRIM | ID: wpr-288098

ABSTRACT

Objective To characterize the biological characteristics of natural vectors of Yesinia (Y.) pestis in China.Methods Species and genera of natural vectors of Y.pestis in China and their faunal distribution were characterized with modem insect taxonomic techniques.The ecological roles of natural vectors of Y.pestis in natural plague foci were determined according to insect ecological experiments.Results There were 63 species of natural vectors of Y.pestis including 28 major reservoirs and 35 secondary ones.Conclusion The biology characteristics of major vectors on Y.pestis and their roles in natural plague foci were defined.

8.
Chinese Journal of Epidemiology ; (12): 692-697, 2012.
Article in Chinese | WPRIM | ID: wpr-288076

ABSTRACT

Objective To study the biological characteristics of natural reservoirs of Yesinia ( Y.) pestis in China.Methods Species and genera of natural reservoirs of E pestis in China and their faunal distribution were characterized with modem taxonomic techniques.The ecological roles of natural reservoirs of Y.pestis in the natural plague foci were determined according to the animal ecological experiments.Results There were 86 species of natural reservoirs of Y.pestis including 14 major reservoirs and 72 kinds of the secondary or occasional reservoirs.Conclusion The biological characteristics of major reservoirs of Y.pestis and their roles in natural plague foci were defined.

9.
Article in Chinese | WPRIM | ID: wpr-635677

ABSTRACT

Background Corneal neovascularization (CNV) is an important cause of visual impairment and graft rejection after allograft corneal transplantation in inflammatory corneal diseases. The mechanisms and therapy relating to CNV are intensely investigated at all times. Objective This study was to evaluate the effect of eicosapentaenoic acid (EPA) on CNV induced by alkali cauterization and its mechanism. Methods The animal models of corneal neovasculation were induced in the right eyes in 72 Sprayue-Dawley rats by putting a piece of 3 mmfilter paper with 1 mol/L NaOH at the center of the cornea for 30 seconds. The rats were then divided randomly into the 0.02 mg EPA treatment group (24 rats) ,0.03 mg EPA treatment group (24 rats) ,model group (24 rats) and normal group (6 rats). EPA of 0.04 ml with doses of 0.02 mg or 0. 03 mg or saline solution of 0. 04 ml was injected subconjunctivally in model rats and immediately after cauterization. The presence of CNV and corneal edema were observed daily by slit lamp biomicroscope. 1,4,7 and 14 days after operation, corneal histopathological examination was performed by hematoxylin and eosin staining. The vascular endothelial cells were stained with CD34 by immunohistochemistry,and the expression of IL-1α,IL-6 mRNA and the nuclear factor-κBp65 ( NF-κBp65 ) proteins was measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. The use of animals complied with the Regulations for the Administration of Affair Concerning Experimental Animals by Hebei Province( version 1998 ). Results Under the slit lamp, CNV grew slowly from days 2-4 with obvious corneal edema and defect of epithelium. Larger CNV area and less edema were seen from days 7-10. Maximal vessel growth was observed 14 days after injury with thinner vessels in the model group. Histological examination showed that part of the corneal epithelium was damaged;serious corneal edema, more inflammatory cells and a lot of CNV in the stroma were presented in the model group. However, repairing of the corneal epithelium without CNV ,light corneal edema and less inflammatory cells were found in both the 0. 02 mg EPA and 0. 03 mg EPA treatment groups 7 days after alkali cauterization. The relative area of CNV in the 0. 02 mg EPA treatment group was ( 15.80±6.43 )% and ( 11.06±2. 14)% ,and that in the 0. 03 mg EPA treatment group was (16. 10±7.41 )% and (11.06±2. 51 )%, showing significant reduction in comparison with the model group [ (84. 74±7.77)% and (89.63±7.50) % ] 7 days and 14 days after operation ( P<0. 05 ). Stronger expression of CD34 in the vascular endothelial cells of the cornea stroma was observed in the model group and an absence of CD34 was observed in the EPA-treated groups on the 7th day. RT-PCR revealed that the expression of IL-1α mRNA and IL-6 mRNA was lower in the EPA treatment groups than the model group ( P<0. 05 ), and Western blot analysis showed that the expression of NF-κB/p65 in the corneas in the EPA treatment groups was significantly lower than that in the model group on the 4th day after operation (P<0.05).Conclusion Topical application of EPA suppresses CNV induced by alkali burn possibly by inhibiting the expression of NF-κB,IL-1α and IL-6.

10.
Chinese Journal of Epidemiology ; (12): 1232-1236, 2011.
Article in Chinese | WPRIM | ID: wpr-241146

ABSTRACT

Objective To study the eco-geographic landscapes of natural plague foci,in China.Methods According to the surveillance records on plague epidemics and the eco-geographic landscapes of natural plague foci based on the county level,the criterion for classifying the ecological geographic zone of Chinese natural plague foci was established.Results 12 types and 19 subtypes of eco-geographic landscapes on Chinese natural plague foci were identified.Conclusion Scientific basis for Chinese natural plague foci classification was provided.

11.
Chinese Journal of Epidemiology ; (12): 989-992, 2009.
Article in Chinese | WPRIM | ID: wpr-321061

ABSTRACT

Objective To investigate Bartonella infection in rodent hosts from different environments and types of climate in Fujian coastal regions. Genetypes of the Bartonella strains was also studied to provide scientific basis for prevention and control of the correlated diseases. Methods By random sampling method, we selected six study sites in Fujian southeastern coastal regions. Rodents were captured by cages to Isolate Bartonella strains. Bartonella-like isolates were confirmed by polymerase chain reaction (PCR). The 379 bp fragment of gltA gene was sequenced and the growth and development tree was constructed to determine Bartonella species. Distribution of Bartonella species in the different area and related hosts was also analysed. Results Bartonella species were isolated from 188 of 1161 small animals including five rodent species. The infected animals were grouped into 2 genera and 2 orders. They were Suncus murinus, Rattas norvegicus, Rnttus flavipectus, Mus masculus and Rattus rattus. The overall prevalence of Bartonella bacteremia was 16.19% in the most prevalent species of rodents in Fujian southeastern coastal regions including 21.43% in Suncus murinus, 13.54% in Rattas norvegicus and 18.27% in Rattus flavipectus. Rodents in every investigated areas were infected by Bartonella species (9.25% in Ningde, 9.52% in Fuzhou, 9.38% in Putian, 28.18% in Quanzhou, 17.42% in Xiamen and 13.33% in Zhangzhou). There were significant differences among infected rates in different annual accumulated temperature districts (χ~2=12.93, P<0.001). Isolates from rodents were clustered in three genotypes (B.elizabethae, B.qeenslandensis and B.tribocorum A, B). Conclusion The local rodents in Fujian southeastern coastal regions were widely infected by Bartonella spp. Differences among the prevalent species of Bartonella in Fujian southeastern coastal region, Yunan and Beijing were noticed. Our findings suggested there was a need to study the prevalence, related vectors and the molecular organism of Bartonella spp.

12.
Article in Chinese | WPRIM | ID: wpr-552593

ABSTRACT

AIM To investigate the possible role of the mitochondrial transmembrane potential (??m) and caspase 3 in arsenic trioxide(As 2O 3)-induced apoptosis of cancer cells. METHOD Namalwa, SGC7901 and Bcap37 cell lines were used as in vitro models. Apoptosis was confirmed by sub-G 1 cells content as well as phosphatidylserine(PS) externalization. The ??m was detected on flow cytometry through double staining of Rhodamine 123(Rh123) and popidium iodide(PI). In addition, the effect of DEVD-CHO, a selective inhibitor of Caspase 3, on As 2O 3-induced apoptosis was studied. RESULT The As 2O 3-induced apoptosis closely associated with the externalization of the ??m and the activation of Caspase 3. As 2O 3 induced cells necrosis when Caspase 3 was inhibited. CONCLUSION As 2O 3 may selectively activates Caspase 3 after it induces externalization of ??m, which causes cancer cells apoptosis.

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