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1.
Article in Chinese | WPRIM | ID: wpr-888363

ABSTRACT

OBJECTIVE@#To explore the genetic basis of four children with congenital hyperinsulinemia (CHI).@*METHODS@#The four children were subjected to high-throughput whole exome sequencing (WES). Candidate variants were validated by Sanger sequencing.@*RESULTS@#WES analysis has identified 4 variants in the ABCC8 gene and 1 variant in GLUD1, including a ABCC8 c.382G>A variant in case 1, compound heterozygous c.698T>C and c.4213G>A variants of the ABCC8 gene concomitant with a de novo 14.9 Mb microduplication of chromosome 15 in case 2, and ABCC8 c.331G>A variant in case 3, and de novo c.955T>C variant of the GLUD1 gene in case 4. Of these, c.698T>C of the ABCC8 gene and c.955T>C of the GLUD1 gene were unreported previously. Based on the American College of Medical Genetics and Genomics guidelines, the c.382G>A(p.Glu128Lys), c.698T>C(p.Met233Thr) and c.4213G>A(p.Asp1405Asn) variants of ABCC8 gene and c.955T>C(p.Tyr319His) variant of GLUD1 gene were predicted to be likely pathogenic(PM1+PM2+PP3+PP4, PM1+PM2+PM5+PP3+PP4, PM1+PM2+PP3+PP4 and PS1+PM1+PM2+PP3), and the c.331G>A (p.Gly111Arg) variant of ABCC8 gene was predicted to be uncertain significance(PM1+PM2+PP4).@*CONCLUSION@#The variants of the ABCC8 and GLUD1 genes probably underlay the pathogenesis of CHI in the four patients. Above results have facilitated clinical diagnosis and genetic counseling for the affected families.


Subject(s)
Child , Genomics , High-Throughput Nucleotide Sequencing , Humans , Hyperinsulinism , Mutation , Whole Exome Sequencing
2.
Chinese Journal of Neonatology ; (6): 264-268, 2019.
Article in Chinese | WPRIM | ID: wpr-753024

ABSTRACT

Objective To study the relationship between single nucleotide polymorphisms (SNPs) of vascular endothelial growth factor A (VEGFA) gene and neonatal necrotizing enterocolitis (NEC).Method From August 2014 to December 2016,preterm infants with a ≥ Ⅱ stage (Modified Bell staging criteria) of NEC admitted to our hospitals were assigned as NEC group.Preterm infants without NEC with similar gestational age and body weight during the same period were assigned as the control group.SNPs of VEGFA including rs1005230,rs833067,rs3025010,rs3025035,rs3025036,rs10434,and rs6905288 were analyzed using SEQUENOM MassARRAY platform and multiplex allele-specific PCR.The concentration of VEGFA in the plasma of the two groups was examined using enzyme-linked immunosorbent assay (ELISA).Result A total of 110 infants were reviewed,including 30 infants in NEC group and 80 infants in the control group.The results showed a significant association of the minor allele frequency (MAF) for T in rs1005230 and C in rs833067 with NEC.The frequencies of C/T (OR=4.810,95%CI 1.742~13.278) and C/T-T/T (OR=4.892,95%CI 1.801~13.246) genotypes in rs1005230,and frequencies of T/C (OR=4.373,95%CI 1.578~12.129) and T/C-C/C (OR=4.000,95%CI 1.484~10.828) genotypes in rs833067 were significantly higher in NEC group than the control group (P<0.05).Infants with MAF in rs1005230 and rs833067 had significantly lower plasma level of VEGFA than infants without MAF (P<0.01).Conclusion The SNPs of rs1005230 and rs833067 may be associated with lower level of VEGFA in plasma and higher risk for NEC.

3.
Chinese Journal of Radiology ; (12): 21-25, 2019.
Article in Chinese | WPRIM | ID: wpr-745206

ABSTRACT

Objective To establish and validate a voxel-based method for the quantitative detection of air trapping (AT),and to explore its diagnostic value by preliminarily apply this method in chronic obstructive pulmonary disease (COPD) patients.Methods From March 2015 to February 2016,fifty healthy young volunteers and eighteen COPD patients who underwent both end-inspiratory and end-expiratory CT were included from the Digital Lung Multi-center Study.The quantitative parameters of AT and emphysema were measured by both the voxel-based quantitative method and the conventional threshold method,respectively.All subjects underwent pulmonary function examination within 3 days after CT examination.For healthy volunteers,paired sample rank-sum test was used to compare the difference of quantitative parameters between voxel-based method and threshold method,Spearman rank correlation analysis was used to investigate the correlation between quantitative parameters of the two methods and pulmonary function.For COPD patients,the distribution and extent of AT and emphysema in patients with similar degree of pulmonary function (PFT) injury were observed.Results There were varying degrees of AT in the asymptomatic youth,with a median value of 5.70% for the voxel-based method and with a median value of 7.96% for the conventional threshold method,there was significant difference(Z=-4.015,P<0.001).The correlation between AT and emphysema parameters of the voxel-based method and PFT parameters (r=-0.399 and-0.494,-0.335 and-0.439 separately,P<0.05) were higher than that of the conventional threshold method,respectively (r=-0.357 and-0.453,-0.284 and-0.391,respectively;all P<0.05).Furthermore,the voxel-based method can classify COPD patients with similar degree of pulmonary function injury into three subtypes:AT-dominant,emphysema-dominant,and mixed.Conclusions The voxel-based AT quantitative measurement method not only has high sensitivity and accuracy,but also provides imaging phenotype for the diagnosis of COPD and provides assistant decision-making for clinical management.

4.
Chinese Journal of Zoonoses ; (12): 293-299, 2017.
Article in Chinese | WPRIM | ID: wpr-610543

ABSTRACT

In order to investigate the molecular evolution and spatio-temporal migration of Getah viruses (GETV) isolated around the world,the nucleotide and deduced amino acid sequence of GETVs were analyzed and phylogenetic trees were constructed by using informatics software including ClustalX1.83,MegaAlign,GeneDOC and Mega6.0.The Bayesian Stochastic Search Variable Selection (BSSVS) program in the BEAST v 1.8.1 software package was used to analyze the spatial dynamics of the Getah virus.Results showed that the full-length of Getah virus E2 gene consists of 1 266 nueleotides,encoding 422 amino acids.And the homology of nucleotide and amino acid were 94.5% 100% and 96.4% 100% respectively.The molecular evolution analysis revealed that there were no species and geographical distribution difference existing among GETV host animals (e.g.horses and pigs) and vectors (e.g.mosquitoes).Bioinformatics analysis showed that GETV originated in Malaysia,then it was spread to Japan,China,South Korea,Mongolia,Russia,etc.GETV E2 gene was relatively stable since GETV was first isolated in 1955.The differences of species and geographical distribution did not exist among GETV host animals and vectors,and the virus has spread from tropical regions to Eurasian continent.Thus,strengthening the detection and monitoring of GETV and its infections in humans and livestock is critical.

5.
Article in Chinese | WPRIM | ID: wpr-808476

ABSTRACT

Objective@#To investigate the distribution patterns of arboviruses in Yunnan province near the China-Laos-Myanmar border, China, and to provide evidence for prevention and control of arboviruses diseases.@*Methods@#Mosquito samples were collected in Daluo county of Xishuangbanna Dai Autonomous Prefecture and Zhengdong county of Pu’er city in Yunnan province, 2012. Viruses were isolated from the samples by tissue culture, positive isolates were identified by RT-PCR with arbovirus species-specific primers, for further sequencing and phylogenetic analysis.@*Results@#A total of 17 species of mosquitoes from 6 genera were collected. A total of 24 strains of viruses were isolated from the mosquito pools and identified as Tembusu virus (TMUV) (2 strains), Japanese encephalitis virus (JEV) (3 strains), Getah virus (GETV) (2 strains), Banna virus (BAV) (4 strains), Densovirus (DNV) (9 strains) and Nam Dinh virus (NDiV) (3 strains).@*Conclusions@#The China-Laos-Myanmar border of Yunnan province is rich in species of mosquitoes and arboviruses.

6.
Chongqing Medicine ; (36): 4666-4668, 2016.
Article in Chinese | WPRIM | ID: wpr-513869

ABSTRACT

Objective To evaluate the value of monitoring circular blood interleukin(IL)-17 for predicting 60 d mortality rate in premature infants with necrotizing enterocolitis(NEC).Methods A total of 85 neonates with surgically or medically treated NEC were retrospectively included.Serum IL-17 level was detected in diagnosing NEC and before preparing operation for evaluating its predictive value.Results The mortality rate after 60 d was 20 % (17/85),in which 5 (10 %)was in the medical treatment group and 12(34%) in the surgical treatment group.The IL-17 level was significantly correlated with mortality rate after 30 d [odds ratio (OR):1.38;CI:1.14-1.67;P=0.001].The preoperative IL-17 level in the surgical treatment group was 27-7 500 pg/mL(median 2 625 pg/mL),which was significantly higher than mean 156 pg/mL in the medical treatment group.The difference in the area under curve(AUC)between the medical treatment group and surgical treatment group was 0.82(CI:0.74-0.90).The critical value of IL-17 for detection significance was 1 783 pg/mL(sensitivity was 90.5%,specificity was 59.2%).Conclusion Serum IL-17 level is correlated with the mortality rate after 60 d.

7.
Chongqing Medicine ; (36): 5142-5144, 2016.
Article in Chinese | WPRIM | ID: wpr-506835

ABSTRACT

Objective To investigate the prevalence of congenital digestive tract malformation in Foshan city ,and to investi‐gate the influential factors on congenital digestive tract malformation .Methods A total of 17 hospitals in Foshan city were moni‐tored from 2011 to 2014 ,the data of digestive tract malformation defects of neonates were analyzed .Results A total of 308 530 newborn infants were monitored ,among them ,189 cases were diagnosed with digestive tract malformation ,the incidence rate was 0 .61‰ .The high risk factors for digestive tract malformation were as follows :infant‐mother aged or more than 30 years old ,smok‐ing or passive smoking ,adverse pregnancy history of infant‐mother ,male gender of neonate and infant born at full term . Conclusion The age of infant‐mother ,smoking or passive smoking ,adverse pregnancy history of infant‐mother ,gender of neonate and gestational age could influence the incidence of gastrointestinal malformations .

8.
Article in Chinese | WPRIM | ID: wpr-503915

ABSTRACT

Objective To observe the effect of intravitreal triamcinolone acetonide injection on choroidal neovascularization in brown Norway rats. Methods Thirty-six healthy brown Norway rats were divided randomly into control group (n=18) and experimental group (n=18), while one eye was chosen randomly as experimental eye. The choroidal neovascularization model was establishied, while 8μl triamcin-olone acetonide was injected in vitreous body immediately after photocoagulation in the experimental group, and the same volume isotonic balanced salt solution was injected in the control group. The eyes of six rats were enucleated for histological slices in the second, forth, and sixth week, respectively. The expression of nuclear factor-kappa B was detected with immunohistochemical method, and the max area of choroidal neovascularization was also measured. Results The area of choroidal neovascularization was smaller, and the expression of nucle-ar factor-kappa B was lower in the experimental group than in the control group (t>7.450, P<0.001). Conclusion Triamcinolone acetonide could effectively inhibit the development of choroidal neovascularization, and decrease the expression of nuclear factor-kappa B in brown Norway rats.

9.
Chinese Journal of Dermatology ; (12): 809-811, 2016.
Article in Chinese | WPRIM | ID: wpr-501859

ABSTRACT

Objective To assess the in vitro antimite activity of artemether against Demodex folliculorum, and to provide evidence for the use of artemether in the treatment of skin diseases caused by Demodex folliculorum infection. Methods Artemether was diluted to different concentrations(20, 10, 5 and 2.5 g/L)with peanut oil. The pH values of working solutions of artemether and peanut oil were measured. Demodex folliculorum mites were divided into several groups(32 mites in each group)to be treated with artemether(20, 10, 5 and 2.5 g/L, artemether groups) or peanut oil(control group). Results There were significant differences in the time required for killing of Demodex folliculorum among the 20?, 10?, 5?and 2.5?g/L artemether groups and control group(Median[P25-P75]:3.00[2.00-3.88]vs. 6.00[4.13- 7.25]vs. 13.00[11.63- 14.50]vs. 17.00[15.25- 20.75]vs. 34.00[23.50- 39.50]hours, H=133.954, P 0.05). Moreover, the pH values of working solutions of artemether and peanut oil ranged between 7.0 and 7.1, and were close to neutral. Conclusion Artemether at 20, 10, 5 and 2.5 g/L can kill Demodex folliculorum in vitro, so artemether may serve as an alternative drug for the treatment of Demodex folliculorum infection.

10.
Article in Chinese | WPRIM | ID: wpr-494294

ABSTRACT

Objective To study the expression and significance of Ezrin in triple negative breast cancer tissues .Methods We selected 102 cases ,including 24 ones of triple negative breast cancer ,58 ones of non‐triple negative breast cancer ,and 20 of benign breast disease .The expression of Ezrin in all the specimens was detected by SP immunohistochemistry .We observed whether there was any difference between the positive expression rates of Ezrin in the three groups . We also analyzed the correlation between Ezrin expression and clinicopathologic parameters of triple negative breast cancer .Results The positive expression rate of Ezrin in groups of triple negative breast cancer , non‐triple negative breast cancer , and benign breast disease was 15 .00% ,48 .28% and 75 .00% ,respectively . The difference between the three groups differed significantly ( P 0 .05 ) . Conclusion Ezrin is highly expressed in triple negative breast cancer tissues ;therefore , it can be used as an important indicator of poor prognosis of triple negative breast cancer .

11.
The Journal of Practical Medicine ; (24): 1598-1601, 2016.
Article in Chinese | WPRIM | ID: wpr-493629

ABSTRACT

Objective To explore the diagnostic value of transvaginal three-dimensional ultrasound in uterus abnormal bleeding caused by uterine incision diverticulum after cesarean section. Methods We collected 480 cases with cesarean section patients from January 2011 to July 2015 in our hospital. A retrospective analysis of the cases was performed by transvaginal ultrasound , if the incision diverticulum was found , for measuring and recording the depth , length , width and assessing the residual muscle layer thickness and the shape and volume of the diverticulum with vaginal bleeding in the following up. Results 118 cases were found incision diverticu-lum by ultrasound in 480 patients , including 70 cases of triangular , 33 cases of semicircle , 15 cases of other shapes , of which 56 patients of uterine abnormal bleeding were significantly correlated with the volume and depth of the diverticulum. While menstruation PBAC scores were higher , the greater probability associated with abnormal uterine bleeding , but bleeding did not associate with the shape and length and width of diverticula; In this study we also found that OR value (OR = 15.809) of the diverticulum volume maximum was the key factors leading to the abnormal uterine bleeding in uterine incision diverticulum. Conclusion Transvaginal three-dimen-sional ultrasound can be convenient , fast and accurate diagnosis of uterine abnormal bleeding caused by incision diverticulum , and is of important significance for guiding clinical medication and surgical treatment.

12.
Chinese Journal of Virology ; (6): 264-268, 2015.
Article in Chinese | WPRIM | ID: wpr-280263

ABSTRACT

To explore the spatial distribution mechanism of Japanese encephalitis virus (JEV), PhyML v3.0 was used to build phylogenetic tree using JEV sequences in the dataset. PAUP v4.0 and Migrapyhla softz ware were then used to analyze the migration events. The results showed that a total of 95 migration events were observed during the dispersal of JEV throughout Asia. Further analysis revealed that Thailand, and several Chinese provinces (including Shandong, Shanghai, Sichuan and Yunnan), were the main migration sources of JEV. JEV spread from these migration sources as follows: from Thailand to Australia, Cambodia, Tibet and India; from Shanghai to eastern coastal Asian regions and Yunnan; from Shandong to Korea, Zhejiang, Hubei, Shanxi and Liaoning; from Sichuan mainly to inland regions of China, as well as Vietnam and Japan; and from Yunnan to Zhejiang. This study indicated that frequent migration events occurred during the dispersal of JEV in the Asia and Pacific regions, and that Thailand, Shandong, Shanghai, Sichuan and Yunnan were the sources of JEV dispersal.


Subject(s)
Asia , Epidemiology , China , Epidemiology , Encephalitis Virus, Japanese , Classification , Genetics , Physiology , Encephalitis, Japanese , Epidemiology , Virology , Phylogeny
13.
Chinese Journal of Zoonoses ; (12): 212-215, 2015.
Article in Chinese | WPRIM | ID: wpr-460505

ABSTRACT

The Flanders virus (FLAV) is a number of family Rhabdoviridae ,contains a single‐stranded ,negative‐sense vi‐ral RNA .Here we describe a molecular detection method developed for fast measurement of FLAV based on Taqman RT‐PCR method .In this study ,FLAV specific primers and probe were designed based on the FLAV L gene sequences published in GeneBank .Quantitative standard curve of FLAV TaqMan PCR was also successfully established .The specificity and stability test showed that the system is specific and the coefficient variables were all less than 1 .7% .Quantitative standard curve based on the genomic copy was drawn ,and the lowest detectable limit (LOD) of system was 100 copies/PCR ,with higher sensitivity and stability than that of the conventional RT‐PCR assay targeting the same gene .

14.
Journal of Practical Radiology ; (12): 1022-1025, 2015.
Article in Chinese | WPRIM | ID: wpr-459591

ABSTRACT

Objective To study the feasibility of dual-source CT with dual-energy imaging based on single contrast enhancement. Methods The clinical data of 60 cases of patients who underwent dual-energy CT (DECT)examination of the heart in our hospital were analyzed.statistically.Results The success rate of 60 cases were 100% for displaying all 600 coronary artery segments at all stages.The total mean score of image quality was 4.68 ±0.57,and the good image rate reached 95%.6 cases of patients who ac-cepted DSA examination were unanimous with DECT findings;In 6 cases of patients who accepted SPECT examination,60 segments were no perfusion abnormalities,but DECT iodine figures of 54 segments were normal,and 6 segments showed perfusion defect. Conclusion DECT with dual-energy imaging based on single contrast enhancement can get excellent coronary and myocardial perfu-sion imaging in the circumstance of appropriate heart rate,which has some potentials for clinical application.

15.
Article in Chinese | WPRIM | ID: wpr-474423

ABSTRACT

Abstract] Objective To analyze the molecular characteristics of the full-length genome of a new-ly isolated genotypeⅤJapanese encephalitis virus (JEV) strain (XZ0934) in China and the first genotypeⅤJEV strain ( Muar) isolated in Malaya 60 years ago.Methods Several softwares including ClustalX 2.0.9, DNAStar 7.1, Bioedit 7.2.5 and MEGA6.06 were used to conduct sequence alignments and phylo-genetic analysis.Results The full-length genomes of XZ0934 strain (isolated in Tibet, China in 2009) and Muar strain (isolated in Malaya in 1952) were composed of 10 983 and 10 988 nucleotides, respective-ly.The XZ0934 strain was highly similar with the Muar strain showing the homology of 90.6%in nucleotide (nt) and 98.3%in amino acid (aa).The open reading frame (ORF) of the two genotype Ⅴ JEV strains encoded 3433 aa while the ORF of other four genotypes (Ⅰ-Ⅳ) (10 299 nt) encoded 3432 aa.Compared with JEV strains of other genotypes, a serine were inserted into the NS4A gene of JEV strains genotype Ⅴand 10-14 nucleotides were inserted into the downstream of the ORF stop codon in 3′-untranslated region. Phylogenetic analysis of E sequences of all JEV strains genotypedⅠ-Ⅴrevealed that in the cluster of geno-typeⅤ, XZ0934 and 10-1827 ( isolated from mosquitoes in South Korea, 2010) stains formed a branch and were divergent from that of Muar strain indicating that there were molecular genetic differences among geno-typeⅤJEV strains after a 60 years hiatus.Conclusion The two genotypeⅤJEV strains showed high lev-els of identity in nucleotide sequences and amino acid sequences with serine insertion in the NS4A gene. However, there were molecular genetic differences between genotypeⅤJEV strains isolated after a 60 years hiatus.

16.
Acta Pharmaceutica Sinica ; (12): 613-20, 2015.
Article in Chinese | WPRIM | ID: wpr-483370

ABSTRACT

In this paper, the new carbon nanotube modified glassy carbon electrode (F-CNTs/GCE) was prepared to establish a new method for studying the molecular interaction mechanism between baicalin metal complexes (BMC) and bovine serum album (BSA), and the principle of this method was discussed deeply. Under the physiological condition, the thermodynamics and kinetics properties of interaction between BMC and BSA were studied by cyclic voltammetry (CV) to inference their molecular effective mechanism. The results show that the presence of F-CNTs can accelerate the electron transfer, and better response signal was showed in the BMC/BMC-BSA system. The detection of interaction of BMC-BSA used new method show that BMC-BSA generates stable thermodynamically non-covalent compounds, and the obtained average binding sites of BMC-BSA were 1.7; the number of electron transfer in BMC/BMC-BSA reaction process was 2, and non electroactive supramolecular compounds of BMC-BSA were generated by this interacting reaction. The relevant research work provides a new way to study the molecular mechanism for the interaction of drugs with protein, and with a certain reference value for discussion on the non covalent interactions.

17.
Article in Chinese | WPRIM | ID: wpr-637707

ABSTRACT

Background Extracellular matrix (ECM) has various kinds of types and important biological function.The important role of ECM during choroidal neovascularization (CNV) arouse attention.Intercellular adhesion molecule-1 (ICAM-1), which is one of ECMs, involves in the formation of blood vessels, but the relationship between CNV and ICAM-1 is still unknown.Objective This study was to observe the dynamic expression of ICAM-1 in krypton laser-induced CNV and explore the effect of ICAM-1 on CNV.Methods Forty-eight healthy male clean BN rats were randomly divided into post-photocoagulation 1-week group,2-week group,3-week group,4-week group, 5-week group, 6-week group, 7-week group and 8-week group.Laser-induced CNV models were monocularly established and the fellow eyes served as the normal controls.Fundus fluorescein angiography (FFA) was carried out to quantitate the leakage degree (absorbency).The eyeballs were enucleated on various time points,and CNV areas were assessed by hematoxylin and eosin staining;immunohistochemistry and in situ hybridization assay were employed, respectively, for the detection of relative expression levels of ICAM-1 protein and mRNA (absorbency).The use and care of the animals complied with the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Committee.Results After laser photocoagulation,the retinal inner and outer nuclear layers were ruptured and invaginated.Disruption of Bruch membrane and macrophages migrating to the photocoagulation zone were seen under the optical microscope.The proliferation of fibroblasts and vascular endothelial cells was exhibited.ICAM-1 protein was mainly expressed in vascular endothelial cells,retinal pigment epithelium (RPE) cells and macrophages,and ICAM-1 mRNA was mainly expressed in the outer nuclear layer.Statistically significant differences were found in the fluorescence leakage degree, the relative expression levels of ICAM-1 protein and mRNA as well as CNV areas among the groups (F=178.839, 739.077,2 463.508,194.434, all at P<0.05).The gradually enlarged CNV area, increased fluorescence leakage, up-regulation of ICAM-1 protein and mRNA expressions were matched with the extension of time after photocoagulation and peaked in the post-photocoagulation 8-week group.Conclusions The expressions of ICAM-1 protein and mRNA up-regulate upon the increase of CNV area and fluorescein leakage, suggesting that ICAM-1 might play an important role in CNV development.

18.
Article in Chinese | WPRIM | ID: wpr-455009

ABSTRACT

OBJECTlVE To investigate the correIation between baicaIin metaI(Ni2+,Co2+,Cu2+) compIexes(BmC)with their anti-tumor activity and the abiIity of BmC to bind to hepatoma ceII DNA. METHODS The cheIating Iigand method was used to synthesize BmC,and the composition and struc-ture of BmC were characterized. mTT,PI staining method and AnnexinⅤ-FITC doubIe staining method were used to anaIyze the effect of BmC on SmmC-7721 ceII proIiferation,cycIe and apoptosis,and to expIore their cytotoxic effect on SmmC-7721 ceIIs in combination with morphoIogy. With DNA extracted from hepatoma ceIIs as a target,cycIic voItammetry and AC impedance were used to study the interaction of BmC with DNA. The interaction mechanism between BmC and DNA was expIored. RESULTS Three new types of BmS were successfuIIy prepared. The moIecuIar formuIas of compIexes were Na2 Ni(C21 H16 O11 )2·10H2 O,Na2 Co(C21 H16 O11 )2·8H2 O,and Na2 Cu(C21 H16 O11 )2·8H2 O,respec-tiveIy. CeII proIiferation and morphoIogy detection reveaIed that BmC 6.25-100 mg·L-1 treatment for 24, 48 and 72 h couId inhibit SmmC-7721 ceII survivaI. BmC cytotoxicity was Iisted as foIIows:baicaIin-cop-per( BC-Cu)﹥ baicaIin-cobaIt( BC-Co)﹥ baicaIin-nickeI( BC-Ni)﹥ baicaIin( BC),in a concentration-dependent manner(P﹤0.01)and time-dependent manner(P﹤0.01). According to the resuIts of ceII cycIe and apoptosis detection,BmC retarded the growth of ceIIs from G0 / G1 phase into S phase or G2 / m phase whiIe inducing apoptosis of SmmC-7721 ceIIs. The resuIts of eIectrochemicaI anaIysis showed that BmC and hepatoma SmmC-7721 ceII DNA formed a non-eIectroactive supermoIecuIar compound through the mixed-mode of eIectrostatic interaction and insertion effect. The binding parameters were obtained:the binding number m = 2,the binding constant βBC = 2.77 ×106 L·moI-1 ,βBC-Ni = 5.46 ×106 L·moI-1 ,βBC-Co =7.74×106 L·moI-1 ,and βBC-Cu =1.21×107 L·moI-1 . The abiIity of BmC to bind to DNA was signifi-cantIy enhanced by BC compIexes with metaI ions,and their abiIity was Iisted as foIIows:BC-Cu﹥BC-Co﹥BC-Ni﹥BC. CONCLUSlON BmC shows cytotoxicity by bIocking the ceII cycIe,inhibiting ceII proIiferation and motivaing apoptosis. The abiIity of BmC to bind to DNA is consistent with its cytotoxicity. BmC,after binding to ceII DNA,wiII bIock DNA repIication,inhibit ceII proIiferation,Iead to ceII apoptosis,and exhibit anti-tumor activity.

19.
Acta Pharmaceutica Sinica ; (12): 337-45, 2014.
Article in Chinese | WPRIM | ID: wpr-448764

ABSTRACT

Chelating ligand method has been used to synthesize baicalin-metal (Ni2+, Co2+, Cu2+) complexes (BMC). The composition and structure of BMC were characterized by the element analysis, ultraviolet spectrum (UV), infrared spectrum (IR), mass (MS) and thermal gravitational analysis (TGA). MTT was used to analyze the effects of BMC on SMMC-7721 cell proliferation. PI staining method and Annexin-V/FITC double staining method were used to analyze the effects of BMC on the cell cycle and apoptosis of SMMC-7721 cell. Fluorescence quantitative RT-PCR was used to analyze the expression of BMC on Bcl-2 gene and Bax mRNA, flow cytometry was used to analyze BMC on the expression of Bcl-2 protein and Bax protein. The antineoplastic activity and mechanism of action of BMC was explored comprehensively. The results showed that three new kinds of BMC (molar ratio of 2 : 1) were successfully prepared, the complexes molecular formula are: Na2Ni(C21H16O11)2 x 10H2O, Na2Co(C21H16O11)2 x 8H2O and Na2Cu(C21H16O11)2 x 8H2O. According to the results of cell cycle and apoptosis detection, BMC stopped cells at G0/G1 phase to S phase and G2/M phase. Gene and protein detection showed that under the given concentration and time, BMC can downregulate the expression of Bcl-2 gene in SMMC-7721 cells, and significantly decrease the expression of Bcl-2 protein, at the same time, with the increase of expression of Bax gene, the Bax protein's expression increased significantly. Which indicates that BMC restrain cell proliferation and cell apoptosis by stopping cell cycle, reducing the expression of Bcl-2 and increasing that of Bax; The anti-tumor activities of three kinds of complexes were: baicalin-copper (BC-Cu) > baicalin-cobalt (BC-Co) > baicalin-nickel (BC-Ni) > baicalin (BC), showing the dose-response relationship.

20.
Modern Clinical Nursing ; (6): 66-67, 2013.
Article in Chinese | WPRIM | ID: wpr-435769

ABSTRACT

Objective To study the effect of SMART principle on the management of nursing research in hospitals. Methods The SMART principle was used in the management of nursing research,including the development,specification and verification of research goals as well as supervision of the process.The number of nurses having published research papers and the number of published papers were calculated during November 2008 to November 2011. Result The number of published papers went down but the number of scientific research papers went up in the year of 2003.Conclusion The SMART principle is effective for promoting the quality of nursing research.

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