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1.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 239-248, 2024.
Article in Chinese | WPRIM | ID: wpr-1003786

ABSTRACT

Parkinson's disease (PD) is a kind of chronic progressive neurodegenerative disease that has a high prevalence rate in recent years, especially in the elderly. PD belongs to the category of "tremor disease" and "tremor" in traditional Chinese medicine, and Tianma Goutengyin is a classic prescription contained in the Synopsis of The New Significance of Patterns and Treatment in Miscellaneous Diseases(《中医内科杂病证治新义》). This article explored the theory of Tianma Goutengyin in the treatment of PD, and based on network pharmacological research, the article summarized relevant research on Tianma Goutengyin and its single herb in the treatment of PD. Moreover, it discussed the clinical applications and mechanisms of Tianma Goutengyin and its single herb in the treatment of PD. It is found that the mechanisms of Tianma Goutengyin in treating PD may be related to resisting oxidative stress, inhibiting inflammatory response, regulating neurotransmitters, and protecting dopamine (DA) neurons. Besides, the main components of the single herb in Tianma Goutengyin for treating PD are gastrodin, rhynchophylline, geniposide, gardenial alcohol, eucommitol glycoside, motherwort alkaloid, baicalin, pachyman, and achyranthes bidentata sterol. They can improve the related symptoms of PD patients by inhibiting inflammatory response, resisting oxidative stress, affecting calcium ion concentration, restoring mitochondrial function, and and protecting DA neurons. This article summarized the research progress of Tianma Goutengyin and its single herb in treating PD, so as to provide a reference for the prescription and medication of Tianma Goutengyin in the treatment of PD and subsequent research on the mechanisms of Tianma Goutengyin and its single herb in the treatment of PD and give play to the advantages of traditional Chinese medicine in the treatment of PD.

2.
Chinese Journal of Blood Transfusion ; (12): 681-686, 2023.
Article in Chinese | WPRIM | ID: wpr-1004764

ABSTRACT

【Objective】 To investigate the compatibility of human albumin and its internal packaging materials of Sinopharm Lanzhou Biopharmaceutical Co., Ltd. 【Methods】 One batch of inner packaging materials (medium borosilicate glass-molded injection bottle and halogenated butyl rubber plug for injection) was extracted with 4 extraction solvents to conduct the toxicological evaluation of potential inner packaging extracts. Through the simulated acceleration test, the trend analysis of the elements in the sample and the inner surface of the glass bottle were observed, and the routine drug inspection items during the long-term stability test process were determined. 【Results】 The detection results of the leaching elements of the internal packaging materials did not exceed the limit of 50%, and the organic matter safety threshold (margin of safety, MOS) was greater than 1.0, indicating that both the leaching elements and the organic matter had no safety risk to the user under the current exposure. The results of the simulated acceleration test show that the drug will not have the risk of peeling tablets after the long-term stability condition was placed for a period of time, and the routine inspection items of the long-term stability test drugs all meet the requirements of the pharmacopoeia. 【Conclusion】 The inner packaging material has no significant impact on the quality of drugs and has good overall compatibility, making it suitable for packaging human albumin.

3.
Journal of Traditional Chinese Medicine ; (12): 2561-2569, 2023.
Article in Chinese | WPRIM | ID: wpr-1003902

ABSTRACT

ObjectiveTo observe the possible mechanism of Xixin Decoction (洗心汤, XXD) in the prevention and treatment of Alzheimer 's disease(AD). MethodsFifty rapid aging model mice (SAMP8) were randomly divided into model group, probiotic group, high-, moderate- and low-dose group of XXD, with 10 mice in each group. Another 10 homologous anti-rapid aging mice (SAMR1) were set as control group. After 10 weeks of feeding, the control group and the model group were given 10 ml·kg-1·d-1 of distilled water by gavage, while the probiotic group (0.39 g·kg-1·d-1), the high-dose group of XXD (5.08 g·kg-1·d-1), the moderate-dose group of XXD (2.54 g·kg-1·d-1), and the low-dose group of XXD (1.27 g·kg-1·d-1) were given corresponding drugs or decoctions by gavage, once a day in all groups. After 10 weeks of intragastric administration, Morris water maze was used to detect the spatial learning and memory ability of mice in each group. HE staining was used to observe the pathological changes of hippocampal CA3 region and colon. The levels of β-amyloid 1-42 (Aβ1-42), lipopolysaccharide (LPS), serum amyloid A (SAA) and acetylcholine (ACH) in hippocampus and colon were detected by ELISA.The diversity of intestinal flora in mouse feces was detected by 16S rRNA sequencing. ResultsCompared to those in the control group, the levels of Aβ1-42,LPS, SAA increased, while the level of ACH decreased in the model group (P<0.05 or P<0.01). Compared to those in the model group, the escape latency period of the probiotic group was significantly shortened on the 2nd and 5th days, while the escape latency period was shortened, and the residence time in the target platform quadrant increased in the high-dose XXD group during the 2nd to 5th days; the escape latency period was shortened significantly in the moderate-dose XXD group on the 5th day (P<0.05 or P<0.01). Compared to those in the model group, the hippocampal neuron cells in the high- and moderate-dose XXD groups were arranged more closely, with decreased levels of SAA, Aβ1-42 and LPS, increased ACH level, Simpson and Shannon index (P<0.05 or P<0.01); the arrangement of hippocampal neuron cells in the probiotic group and the low-dose XXD group was relatively loose; the proportions of Bacteroidetes and Prevotella were significantly reduced in the probiotic group and the high-dose XXD group, while that of Firmicutes and Lactobacillus significantly increased (P<0.05 or P<0.01). Compared to those in the probiotic group and the high-dose XXD group, the number of goblet cells in the moderate-dose XXD group decreased, and the number of glands in the low-dose XXD group decreased with atrophy. The high-dose XXD group had decreased Aβ1-42 level in the hippocampus, increased ACH level in thehippocampus and colon tissue, and decreased SAA in the colon tissue than the moderate- and low-dose XXD groups (P<0.05 or P<0.01); moreover, the SAA level in the hippocampus was significantly higher in the low-dose XXD group than the high- and moderate-dose groups (P<0.01). ConclusionXXD can improve the spatial learning and memory ability of SAMP8, reduce the production and deposition of LPS, SAA and Aβ1-42 in brain and intestine, and increase the content of ACH. The mechanism of its prevention and treatment of AD maybe related to regulating intestinal microecology, affecting flora diversity and improving inflammatory response.

4.
Chinese Journal of Digestive Endoscopy ; (12): 131-139, 2023.
Article in Chinese | WPRIM | ID: wpr-995370

ABSTRACT

Objective:To investigate the risk factors for intraoperative hemorrhage during endoscopic submucosal dissection (ESD) for colorectal lesions.Methods:Data of 386 patients with colorectal lesions, who underwent ESD at The Third People's Hospital of Datong and its cooperative hospital, Nanjing Drum Tower Hospital, from December 2019 to August 2021 were retrospectively analyzed. The patients were divided into the hemorrhage group ( n=85) and the non-hemorrhage group ( n=301) according to intraoperative hemorrhage. The correlationship of patients'basic information, lesion-related factors and hemorrhage during colorectal ESD was analyzed. Univariate and multivariate logistic regression were used to identify the risk factors for intraoperative hemorrhage during ESD. The risk predictive model of intraoperative hemorrhage during ESD was established according to the screened risk factors, and receiver operator characteristic (ROC) curve was used to evaluate the predictive model. Results:Univariate logistic regression showed that a history of diabetes ( OR=2.340, P<0.05), a history of coronary atherosclerotic heart diseases ( OR=3.100, P<0.05), the lesion located in the rectum ( OR=3.272, P<0.05), longer lesion ( OR=1.093, P<0.05), wider lesion ( OR=1.057, P<0.05), larger lesion ( OR=1.126, P<0.05), depressed lesion ( OR=6.128, P<0.05), the laterally spreading lesion ( OR=2.651, P<0.05), the lesion infiltrated into the SM-S layer ( OR=0.088, P<0.05), the lesion infiltrated into the SM-D layer ( OR=0.174, P<0.05), the diameter of hemorrhage vessels 0.5~<1.0 times of the diameter of incision knife ( OR=246.854, P<0.05), the postoperative pathology as early cancer ( OR=7.000, P<0.05) were risk factors for intraoperative hemorrhage during ESD. Considering the quantitative relationship between the length, the width and the area of lesions, multi-factor models were constructed using the length and area of lesions respectively. Forward stepwise regression was used to screen variables and determine the final model, and the results showed that a history of coronary atherosclerotic heart diseases, the depressed lesion, the longer lesion, the larger lesion, the diameter of hemorrhage vessels 0.5~<1.0 times of the diameter of the incision knife were independent risk factors for intraoperative hemorrhage during ESD. The two modeling results of the lesion length and the lesion area were very similar. Therefore, lesion length was recommended to describe lesions in clinical practice. Conclusion:A history of coronary atherosclerotic heart disease, the depressed lesion, the longer lesion, the larger lesion, the diameter of vessels 0.5~<1.0 times of that of the incision knife are independent risk factors for intraoperative hemorrhage during ESD.

5.
Chinese Journal of Blood Transfusion ; (12): 89-92, 2022.
Article in Chinese | WPRIM | ID: wpr-1004053

ABSTRACT

【Objective】 To establish and verify the vacuum decay method for the tightness inspection of blood products. 【Methods】 The method for inspecting the tightness of blood product was established, and the detection limit, linearity, range, accuracy, precision and durability were verified according to the requirements of methodological verification.The validated method was used to check the tightness of blood product packaging. 【Results】 The detection limit of this method was 2.5 μm, linear correlation coefficient was r=1, the differential pressure of positive sample was within the allowable range of accuracy, and the durability met the requirements.The RSD of results of 6 repeatability tests and 12 intermediate precision tests were both less than 10%, and all validation items met the verification standards. 【Conclusion】 Vacuum decay method can be used to check the tightness of blood products.

6.
Chinese Journal of Medical Genetics ; (6): 488-493, 2022.
Article in Chinese | WPRIM | ID: wpr-928443

ABSTRACT

OBJECTIVE@#To conduct clinical and genetic analysis of two male patients with atypical Rett syndrome.@*METHODS@#Collection of clinical data in the two patients and these parents; whole exome sequencing (WES) was used to detect the potential variants, which were verified by Sanger sequencing. X chromosome inactivation (XCI) detection is performed in the Patient 1's mother to detect the allelic expression difference of the MECP2 gene.@*RESULTS@#Patient 1, a 5-year and 10-month-old boy, had mental disorders and mild intellectual disability (ID) (IQ: 54), whose mother had ID. Patient 2 was a 9-month and 18-day-old male presented with recurrent infections, respiratory insufficiency, hypotonia and global developmental delay. WES indentified a hemizygous mutation, c.499C>T (p.R167W), in the MECP2 gene in patient 1, which was inherited from his mother. The inactivation of X chromosome is skewed, and the expression ratio of wild-type and mutant MECP2 is 100%:0. Patient 2 was found a de novo splicing mutation, c.62+2_62+3del in the MECP2 gene. They were both reported pathogenic variant related to Rett syndrome. c.499C>T (p.R167W) was defined as likely pathogenic (PS1+PM2+PP3) and c.62+2_62+3del was pathogenic (PVS1+PM2+PM6) based on American College of Medical Genetics and Genomics standards and guidelines.@*CONCLUSION@#Both the two patients were diagnosed with rare male Rett syndrome, which had atypical clinical manifestations and large difference. Above foundings have revealed novel phenotypes in Chinese male patients with Rett syndrome.


Subject(s)
Female , Humans , Male , Craniosynostoses , Genetic Testing , Intellectual Disability/genetics , Methyl-CpG-Binding Protein 2/genetics , Mutation , Phenotype , Rett Syndrome/genetics
7.
Chinese Journal of Obstetrics and Gynecology ; (12): 339-345, 2022.
Article in Chinese | WPRIM | ID: wpr-932443

ABSTRACT

Objective:To establish a scoring scale for trial of labor after cesarean section (TOLAC), to explore the evaluation ability of this scoring scale for vaginal delivery after cesarean section (VBAC), and to improve the success rate of TOLAC.Methods:The delivery information of 661 TOLAC pregnant women admitted to Zhengzhou Central Hospital Affiliated to Zhengzhou University from 2014 to 2017 was retrospectively analyzed, and the TOLAC scoring scale was established by referring to relevant literatures. A prospective cohort study of pregnant women with TOLAC from January 2018 to December 2019 in Zhengzhou Central Hospital was conducted, including 440 pregnant women who were excluded from contraindications in trial labor. According to TOLAC scoring scale, pregnant women were divided into 3 groups, 0-6 group (94 cases), 7-9 group (234 cases) and 10-15 group (112 cases). The success rate of trial labor, failure reasons and incidence of maternal and neonatal complications were compared among the three groups.Results:(1) The overall success rate of TOLAC in 440 pregnant women was 75.0% (330/440). The success rates of 0-6, 7-9 and 10-15 groups were 53.2% (50/94), 76.9% (180/234) and 89.3% (100/112), respectively. The success rate of 10-15 group were significantly higher than those of 0-6 and 7-9 groups (all P<0.05). (2) Among the causes of trial labor failure, there were statistically significant differences between the three groups in terms of threatened uterine rupture and maternal abandonment (all P<0.05). Pairings showed that the incidences of threatened uterine rupture and maternal abandonment in 0-6 group was lower than those in 7-9 and 10-15 groups, and the differences were statistically significant (all P<0.05). (3) Maternal and neonatal complications mainly included postpartum hemorrhage and neonatal asphyxia, but there were no significant difference in the incidence of TOLAC success or failure among the three groups (all P>0.05). There was no uterine rupture in all groups. (4) The main factors affecting TOLAC score of pregnant women in the three groups included natural labor, estimated weight of the fetus at this time, Bishop score of the cervix at admission and gestational age, and the scores of the above indexes in 10-15 group were significantly higher than those in 0-6 group and 7-9 group (all P<0.05). Conclusions:TOLAC scoring scale has more accurate evaluation ability for VBAC, which could improve the success rate of TOLAC and maternal and child safety. The score of 0-6 is not recommended for vaginal trial labor, the score of 7-9 is recommended for vaginal trial labor, and the score of 10-15 is strongly recommended for vaginal trial labor.

8.
Journal of Public Health and Preventive Medicine ; (6): 141-145, 2022.
Article in Chinese | WPRIM | ID: wpr-920393

ABSTRACT

Objective To evaluate the dynamic characteristics of serum complement C1q, C3 and C4 levels in stroke patients over 85 years of age, and to analyze the effects of these indicators on the prognosis of cardiovascular events, and to explore its influencing factors. Methods A total of 320 stroke (AIS) patients admitted to our hospital from April 2017 to October 2020 were selected. Then, according to the prognosis of cardiovascular events (cardiovascular events and death, cardiovascular events but not fatal, no cardiovascular events), they were divided into 3 groups. At the same time, the serum of outpatients in the hospital was selected as a control. During treatment, 3 days after treatment, and 7 days after treatment, serum complement C1q, C3 and C4 levels were evaluated. The dynamic changes of serum complement C1q, C3 and C4 levels were determined by enzyme-linked immunosorbent assay. Results During the treatment, the levels of complement C1q, C3 and C4 showed a gradual decrease in 3 days after treatment and 7 days after treatment. Cardiovascular events occurred and died, and cardiovascular events occurred but not fatal. In the three groups of patients without cardiovascular events, serum complement C1q, C3 and C4 levels were significantly higher than those of the normal population. The levels of complement C1q, C3 and C4 in the cardiovascular event and fatal group were significantly higher than those in the cardiovascular event but not fatal group, and the complement C1q, C3 and C4 levels in the cardiovascular event but not fatal group were significantly higher than those in the non-cardiovascular event group , The differences between the two groups were statistically significant (P<0.05). Logistic regression analysis showed that the levels of complement C1q, C3 and C4 at the two time points after treatment can be used as independent factors influencing the prognosis of cardiovascular events (increased levels are risk factors). At the same time, the decreasing trend of complement level is a protective factor for the prognosis of adverse cardiovascular events. Conclusion Elevated serum levels of complement C1q, C3 and C4 in stroke patients over 85 years of age indicate a poor prognosis for cardiovascular events. Dynamic monitoring of serum complement C1q, C3 and C4 levels is of great significance to improve the prognosis of patients.

9.
Clinical Medicine of China ; (12): 527-533, 2022.
Article in Chinese | WPRIM | ID: wpr-956413

ABSTRACT

Objective:To investigate the clinical situation of 201 emergency adult sudden death patients, and analyze the influence of white blood cell count and arterial blood lactate level on prognosis.Methods:The clinical data of 201 patients diagnosed with sudden death in the emergency department of Medical College of Cangzhou people's Hospital from January 2017 to January 2021 were retrospectively analyzed. The gender, age, disease composition and etiology of the patients were statistically analyzed. The independent sample t-test was used to compare the measurement data with normal distribution, the χ 2 test or Fisher exact probability method was used to compare the counting data between groups, and the logistic regression model was used to screen the risk factors of emergency death, and the impact of white blood cell count and arterial blood lactate level on the prognosis was analyzed. Results:After active rescue, 11.44% (23/201) of the patients were successfully rescued, and 88.56% (178/201) of the patients were ineffective; ≥46-≤65 years old was the age group with high incidence of sudden death (55.22%(111/201)). The proportion of male (43.28% (87/201), 23.38% (42/201)) in the age group of ≥46-≤65 years old and the age group over 65 years old were higher than that of female (11.94% (24/201), 14.43% (29/201)), with a statistically significant difference (χ 2=4.801, 9.209; P=0.028, 0.002). In the past history of sudden death patients, the proportion of cardiovascular disease (53.23% (107/201)) was the highest; the proportion of patients may have inducements before sudden death was 74.13% (149/201), the proportion of patients have premonitory symptoms before sudden death was 67.66% (136/201), and sudden cardiac death was the first cause. Logistic regression analysis showed that white blood cell count ( OR=4.442,95% CI: 1.898-10.395), arterial blood lactic acid concentration ( OR=4.272,95% CI: 2.024-9.016), and albumin concentration ( OR=2.657,95% CI: 1.302-5.422) were independent risk factors affecting emergency sudden death patients ( P values were 0.001, <0.001, 0.007, respectively). Conclusions:There are some differences in gender, age and past history of adult sudden death patients. Most of them have premonitory symptoms and inducements. Sudden cardiac death is the primary cause. The increases of white blood cell count and lactic acid level, the decrease of albumin level are the risk factors of sudden death.

10.
Sichuan Mental Health ; (6): 580-584, 2022.
Article in Chinese | WPRIM | ID: wpr-987368

ABSTRACT

This article aims to provide a review of the domestic and foreign studies on the quality indicators for evaluating the psychiatric nursing care in recent years. According to Donabedian model, the research progress of quality indicators for evaluating the psychiatric nursing care were described from the three dimensions of structure quality, process quality and outcome quality. This paper summarizes the status of the research, points out the shortcomings of existing research, and provides references for the future research on the quality indicators for evaluating the psychiatric nursing care.

11.
Chinese Journal of Blood Transfusion ; (12): 1147-1149, 2021.
Article in Chinese | WPRIM | ID: wpr-1004318

ABSTRACT

【Objective】 To establish a microbial limit test method for diatomite and pearlite, and verify its applicability. 【Methods】 According to the requirements of general rule 1105, Microbial Limit Test for Non Sterile Products of Pharmacopoeia of the People′s Republic of China (2020 Edition), the applicability test of microbial counting methods for three batches of perlite and diatomite was conducted before the microbial limit test of samples. The microbial growth of filter aid was analyzed and the recovery rate of each test bacterium was calculated. 【Results】 The ratio of the colony number of the test group minus the colony number of the test sample control group to the bacterial liquid control group was in the range of 0.5~2.0. 【Conclusion】 The method is accurate, reliable and can be used for microbial limit test of diatomite and perlite.

12.
Chinese Journal of Radiological Medicine and Protection ; (12): 469-474, 2021.
Article in Chinese | WPRIM | ID: wpr-910341

ABSTRACT

Pelvic radiotherapy is a way for treatment of most pelvic tumors, of which the pelvic insufficiency fracture (PIF) is a long-term complication. In this review, research progress of pelvic insufficiency fracture is summarized and discussed. The pathogenesis of PIF is mainly about inhibition of osteoblasts and the risk factors of PIF include old age, postmenopausal status, absence of hormonal replacement therapy, high number of births, smoking history, low body mass index (BMI), concurrent rheumatoid arthritis, concurrent diabetes mellitus, intracavitary brachytherapy of the high dose rate (HDR-ICBT), high dose of radiotherapy, etc. Effective drugs for prevention or treatment of PIF have not been found yet. Delayed diagnosis and misdiagnosis of PIF can cause serious consequences. As a result, further studies are needed to guide clinical work.

13.
International Journal of Traditional Chinese Medicine ; (6): 1264-1268, 2021.
Article in Chinese | WPRIM | ID: wpr-907703

ABSTRACT

Acupuncture has achieved good effect in the treatment of various types of cervical spondylosis, which can alleviate the symptoms of neck pain and vertigo, and improve the blood supply to the brain. In terms of acupuncture location, treatment mainly selects acupoints according to meridians and syndrome differentiation, that is, identifying the diseased meridians and giving acupuncture treatment according to massage along meridians and pressing in the traditional meridian diagnosis method,or taking points according to location, including near and far ends based on meridian theory, acupuncture needlingcorresponding locations of hands and feet based on holographic theory, or taking points according to innervated areas or muscle fibers based on anatomical structure. Acupuncture methods mainly include warming and dredging acupuncture, short needling, fast needling and so on. In addition to filiform needles, floating needles, long needles, electroacupuncture and other special needles arealso usedin clinic.

14.
Chinese Journal of Medical Science Research Management ; (4): 284-291, 2021.
Article in Chinese | WPRIM | ID: wpr-912612

ABSTRACT

Objective:This study analyzes COVID-19 prevention and treatment technology patents from 103 countries and regions worldwide, in order to have a good grasp of the most-current trends in COVID-19 prevention and treatment technology research worldwide, discuss the research directions of such technologies, provide theoretical foundations for interdisciplinary and multi-team cooperation.Methods:The retrieval strategies were constructed utilizing the retrieval and analysis system by the Regional Patent Information Service (Nanjing) Center of The State Intellectual Property Office and IncoPat's scientific and technological innovation platform, using third-level branches as the unit and separating the retrieval and proof processes, the queries built using IPC classification numbers and key words; the search was limited to invention patents, with utility models and designs excluded.Results:On February 14, 2020, there had been a total number of 136 087 invention patent applications for COVID-19 prevention and treatment technologies globally. In terms of the country of origin, the U. S. ranked first with 56 095 patents, followed by China (18 096). In terms of technical field, the top three were chemical therapeutic agents (47 634), vaccines (47 248) and diagnostic tests (12 991), with the U. S. possessing absolutely advantageous patent portfolios in all three fields. The applicants were mainly comprised of large enterprises abroad and universities and research institutes in China.Conclusions:China′s COVID-19 related patent applications are relatively scattered. In the field of chemotherapy, there is room for improvement compared with the advanced countries, and cooperation between scientific institutions and enterprises is insufficient. Therefore, China should further increase investment in chemical drug research and development and seize the opportunity to actively promote the research and development of vaccine technology. On the other hand, we need to consolidate the technical advantages and product advantages of epidemic prevention and control which are already accumulated, and strengthen the protection and layout of intellectual property rights. Furthermore, the technological innovation system combining production, education and research is the breakthrough point to encourage enterprises to become the subject of technological innovation.Last but not least, we should build a service platform for the transfer and transformation of scientific and technological achievements to enhance intellectual property rights and promote the transformation of scientific and technological achievements.

15.
Cancer Research on Prevention and Treatment ; (12): 333-340, 2021.
Article in Chinese | WPRIM | ID: wpr-988373

ABSTRACT

Objective To observe the expression difference of lncRNA FAL1 in ovarian cancer cells and their drug-resistant cell lines, and to explore the effect and mechanism of lncRNA FAL1 down-regulation on cell chemotherapy resistance. Methods The expression levels of fal1 gene in SKOV3 and COC1 cells and their drug-resistant cell lines were detected by qRT-PCR. fal1 siRNA was transfected to downregulate fal1 gene expression. MTT was used to detect cell proliferation. Transwell method was used to detect cell invasion ability. Plate clone formation test was used to detect cell clone ability, and Western blot was used to detect MDR-1, mpr-1, ABCG2 and phosphorylation levels of p38 MAPK, ERK1/2 and JNK. SKOV3/DDP and COC1/DDP cells transfected with FAL1-siRNA were injected subcutaneously into BALB/c nude mice. The volume and mass of subcutaneous transplanted tumors were measured. Results Compared with SKOV3 and COC1 cells, SKOV3/DDP and COC1/DDP cells were less sensitive to DDP, and the expression levels of FAL1 gene increased (P < 0.01). After transfection with FAL1-siRNA, the sensitivity of SKOV3/DDP and COC1/DDP cells to DDP increased (P < 0.01), and the invasion (P < 0.05) and cloning ability (P < 0.01) decreased. The expression levels of MDR-1, MPR-1, ABCG2 (P < 0.01) and the phosphorylation levels of p38 MAPK, ERK1/2 and JNK (P < 0.05) decreased. The volume and mass of subcutaneous transplanted tumors were significantly reduced (P < 0.01). Conclusion Down-regulation of lncRNA FAL1 could significantly reduce the chemotherapy resistance of cisplatin-resistant ovarian cancer cell lines and inhibit the proliferation of drug-resistant cells in vivo. Its mechanism is related to inhibiting the activation of MAPK signaling pathway.

16.
Sichuan Mental Health ; (6): 75-78, 2021.
Article in Chinese | WPRIM | ID: wpr-987572

ABSTRACT

The purpose of this paper is to optimize the course construction and teaching process of psychopharmacology, and analyze the problems in the course of teaching and assessment of psychopharmacology from many aspects. This article is to deeply excavate the space for improvement, and enrich the teaching links by using existing conditions, technology and personnel to enhance the teaching effect and improve the teaching quality, so as to provide references for the reform of similar course teaching.

17.
Sichuan Mental Health ; (6): 469-474, 2021.
Article in Chinese | WPRIM | ID: wpr-987492

ABSTRACT

ObjectiveTo investigate the current status of knowledge, attitude and practice of nurses in mental health hospitals on the prevention and control of nosocomial infections, and to analyze the influencing factors. MethodsFrom June to August 2020, 402 clinical nurses from 8 mental health hospitals in a city were enrolled using simple random sampling method, and were assessed using a self-compiled knowledge-attitude-practice questionnaire related to the nosocomial infection prevention and control, thereafter, the influencing factors were screened. ResultsThe scores of knowledge, attitude and practice dimensions in relation to the nosocomial infection prevention and control among the nurses were (8.43±1.84), (76.01±5.70) and (57.42±3.75), respectively. Univariate analysis showed that the knowledge dimension score demonstrated significant difference among nurses of different types of hospital, grades of hospital, professional titles, education levels, and emphasis degrees of hospital and individual on nosocomial infection (P<0.05 or 0.01). The attitude dimension scores yielded significant difference among nurses of different types of hospital, position, grades of hospital, and emphasis degrees of hospital and individual on nosocomial infection (P<0.05 or 0.01). The practice dimension scores manifested significant difference among nurses of different types of hospital, grades of hospital, professional titles, emphasis degrees of hospital and individual on nosocomial infection, and training frequency (P<0.05 or 0.01). Multiple linear regression analysis showed that professional titles and individual emphasis on nosocomial infection entered the regression model of nurses' knowledge dimension of nosocomial infection prevention and control (P<0.05 or 0.01). Individual emphasis on nosocomial infection, hospital types and position entered the regression model of attitude dimension (P<0.05 or 0.01). Individual emphasis on nosocomial infection, hospital types, professional titles, and hospital emphasis on nosocomial infection entered the regression model of practice dimension (P<0.05 or 0.01). ConclusionNurses in mental health hospital have a positive attitude towards nosocomial infection prevention and control, but their knowledge base needs to be strengthened.

18.
China Pharmacy ; (12): 581-585, 2020.
Article in Chinese | WPRIM | ID: wpr-817314

ABSTRACT

OBJECTIVE:To establish th e method for the determination of related substances in fidaxomicin raw material. METHODS:The detection ability of NP-HPLC-UV ,RP-HPLC-ELSD and RP-HPLC-UV systems for the related substances in fidamycin raw material was investigated and the best chromatographic system was selected . The HPLC detection method for the related substances was established. The detection was performed on Agilent Eclipse XDB C 18 column with mobile phase A consisted of 0.2% triethylamine buffer solution (pH 3.8)-acetonitrile(55∶45,V/V),mobile phase B consisted of 0.2% triethylamine buffer solution(pH 3.8)-acetonitrile(20∶80,V/V)at the flow rate of 1.0 mL/min(gradient elution );the detection wavelength was set at 230 nm,and column temperature was 35 ℃;the sample size was 10 µL. Calculation of the content of related substances was principal component self-control method without correction factor. RESULTS :The impurities C and F could not be separated effectively in NP-HPLC-UV system. In RP-HPLC-ELSD system ,only impurities C ,D,E and F could be detected. In RP-HPLC-UV system ,11 impurities could be detected. In the study of methodology ,the linear ranges were 0.5-20.0 μg/mL for fidaxomicin(R2=0.999 9);the LOD was 0.05 ng,LOQ was 0.15 ng;RSDs of reproducibility and intermediate precision tests were less than 2.0%(n=6);average recovery was 98.4%(RSD=3.6%,n=9). The sum of impurities in 3 batches of raw materials were 0.53%,0.51%,0.51%,respectively. CONCLUSIONS :The effect of detecting impurities by RP-HPLC-UV are the best. Established method is specific and sensitive ,and can be used for the determination of related substance in fidaxomicin raw material.

19.
Western Pacific Surveillance and Response ; : 5-10, 2020.
Article in English | WPRIM | ID: wpr-877114

ABSTRACT

Abstract@#During the yellow fever epidemic in Angola in 2016, cases of yellow fever were reported in China for the first time. The 11 cases, all Chinese nationals returning from Angola, were identified in March and April 2016, one to two weeks after the peak of the Angolan epidemic. One patient died; the other 10 cases recovered after treatment. This paper reviews the epidemiological characteristics of the 11 yellow fever cases imported into China. It examines case detection and disease control and surveillance, and presents recommendations for further action to prevent additional importation of yellow fever into China.

20.
Journal of Xi'an Jiaotong University(Medical Sciences) ; (6): 931-935, 2020.
Article in Chinese | WPRIM | ID: wpr-843831

ABSTRACT

Objective: To study the characteristics and regularity of the improvement of early clinical symptoms of coronavirus disease 2019 (COVID-19) treated with Chinese medicine plus fumigation and absorption combined with super dose of vitamin C. Methods: We randomly divided 30 patients diagnosed with COVID-19 admitted by the Hubei medical team in our hospital since February 2020 into groups A, B and C, with 10 cases in each group. Group A was the control group. Group B was treated with traditional Chinese medicine and fumigation. Group C was the treatment group of traditional Chinese medicine plus fumigation and absorption combined with super dose of vitamin C. We analyzed the symptoms of fatigue, cough, dry throat, shortness of breath and the improvement of chest CT and nucleic acid detection, and compared the treatment status of each group. Results: The improvement degree and disappearance time of fatigue, cough, dry throat and shortness of breath in group B and group C were better than those in group A, and the effect of group C was better than that of group B (P0.05). Conclusion: The combination of traditional Chinese medicine and fumigation and absorption combined with super dose of vitamin C has a definite effect on the improvement of fatigue, cough, dry throat and shortness of breath in patients with COVID-19.

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