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1.
Acta Physiologica Sinica ; (6): 265-275, 2022.
Article in Chinese | WPRIM | ID: wpr-927602

ABSTRACT

Group 3 innate lymphoid cells (ILC3) as a family member of innate lymphoid cells (ILCs), have been defined as novel innate immune cells in the past decade. ILC3 include a variety of heterogenous subsets with different phenotypes and functions, which are mainly distributed in barrier organs such as the intestine, lung and skin. They play an important role in immune regulation, tissue repair and lymphoid tissue formation. However, in various inflammatory diseases, ILC3 become dysregulated and participate in the pathogenesis through secreting a series of cytokines such as interleukin (IL)-17, IL-22, interferon-γ (IFN-γ) and granulocyte-macrophage colony-stimulating factor (GM-CSF) to modulate other immune cells and induce the formation of ectopic lymphoid structures. Therefore, it is of great significance to explore the phenotype and function of ILC3 in order to advance the understanding of inflammatory diseases and find new therapeutic targets. In this article, the phenotypic characteristics, biological functions and research progress of ILC3 in inflammatory diseases were reviewed.


Subject(s)
Cytokines , Immunity, Innate , Interferon-gamma , Intestines , Lymphocytes
2.
Acta Pharmaceutica Sinica ; (12): 1459-1464, 2022.
Article in Chinese | WPRIM | ID: wpr-924747

ABSTRACT

A method to measure the antibody-dependent cell-mediated phagocytosis (ADCP) potency of anti-CD38 mAb was developed based on design of experiment (DoE) with a Jurkat/NFAT/CD32a-FcεRIγ transgenic cell line as the effector cell, the Daudi cell line as the target cells, and luciferase as the detection system. The DoE method was used for optimization of experimental parameters and methodological validation. The results show that anti-CD38 mAb exhibits a dose-response relationship with the following four-parameter equation: y = (A - D) / [1 + (x / C)B] + D. Several experimental parameters were optimized by statistical experimental design and determined as follows: the working concentration of anti-CD38 mAb was 800-20.81 ng·mL-1, the density of the target cells was 7.5×104 per well, and the density of effector cells was 2.5×104 per well, with an induction time of 6 h. The method showed good specificity. The recovery rate for samples from 5 different groups showed that the relative potencies of anti-CD38 mAb were (59.97 ± 4.74) %, (82.44 ± 5.15) %, (110.69 ± 11.71) %, (129.23 ± 5.22)% and (162.15 ± 3.66) %. The recoveries ranged from 103% to 120% and the RSDs of the above results were all less than 11%. The linear detection range was 50%-150%. Based on DoE design, this method for measuring ADCP potency of anti-CD38 mAb was optimized and validated with good specificity, repeatability and accuracy. This method can be used for evaluation of ADCP biological activity of anti-CD38 mAbs.

3.
Article in English | WPRIM | ID: wpr-892680

ABSTRACT

Background/Aims@#Depressive symptom is one of the most common symptoms in patients with irritable bowel syndrome (IBS), but its pathogenetic mechanisms remain unclear. As a voxel-level graph theory analysis method, degree centrality (DC) can provide a new perspective for exploring the abnormalities of whole-brain functional network of IBS with depressive symptoms (DEP-IBS). @*Methods@#DC, voxel-wise image and clinical symptoms correlation and seed-based functional connectivity (FC) analyses were performed in 28 DEP-IBS patients, 21 IBS without depressive symptoms (nDEP-IBS) patients and 36 matched healthy controls (HC) to reveal the abnormalities of whole brain FC in DEP-IBS. @*Results@#Compared to nDEP-IBS patients and HC, DEP-IBS patients showed significant decrease of DC in the left insula and increase of DC in the left precentral gyrus. The DC’s z-scores of the left insula negatively correlated with depression severity in DEP-IBS patients.Compared to nDEP-IBS patients, DEP-IBS patients showed increased left insula-related FC in the left inferior parietal lobule and right inferior occipital gyrus, and decreased left insula-related FC in the left precentral gyrus, right supplementary motor area (SMA), and postcentral gyrus. In DEP-IBS patients, abstracted clusters’ mean FC in the right SMA negatively correlated with depressive symptoms. @*Conclusions@#DEP-IBS patients have abnormal FC in brain regions associated with the fronto-limbic and sensorimotor networks, especially insula and SMA, which explains the vicious circle between negative emotion and gastrointestinal symptoms in IBS. Identification of such alterations may facilitate earlier and more accurate diagnosis of depression in IBS, and development of effective treatment strategies.

4.
Article in English | WPRIM | ID: wpr-900384

ABSTRACT

Background/Aims@#Depressive symptom is one of the most common symptoms in patients with irritable bowel syndrome (IBS), but its pathogenetic mechanisms remain unclear. As a voxel-level graph theory analysis method, degree centrality (DC) can provide a new perspective for exploring the abnormalities of whole-brain functional network of IBS with depressive symptoms (DEP-IBS). @*Methods@#DC, voxel-wise image and clinical symptoms correlation and seed-based functional connectivity (FC) analyses were performed in 28 DEP-IBS patients, 21 IBS without depressive symptoms (nDEP-IBS) patients and 36 matched healthy controls (HC) to reveal the abnormalities of whole brain FC in DEP-IBS. @*Results@#Compared to nDEP-IBS patients and HC, DEP-IBS patients showed significant decrease of DC in the left insula and increase of DC in the left precentral gyrus. The DC’s z-scores of the left insula negatively correlated with depression severity in DEP-IBS patients.Compared to nDEP-IBS patients, DEP-IBS patients showed increased left insula-related FC in the left inferior parietal lobule and right inferior occipital gyrus, and decreased left insula-related FC in the left precentral gyrus, right supplementary motor area (SMA), and postcentral gyrus. In DEP-IBS patients, abstracted clusters’ mean FC in the right SMA negatively correlated with depressive symptoms. @*Conclusions@#DEP-IBS patients have abnormal FC in brain regions associated with the fronto-limbic and sensorimotor networks, especially insula and SMA, which explains the vicious circle between negative emotion and gastrointestinal symptoms in IBS. Identification of such alterations may facilitate earlier and more accurate diagnosis of depression in IBS, and development of effective treatment strategies.

5.
Acta Pharmaceutica Sinica ; (12): 2276-2281, 2021.
Article in Chinese | WPRIM | ID: wpr-887037

ABSTRACT

The high performance liquid chromatography-fluorescence micelle assay (HPLC-FMA) method for the content determination of polysorbate 80 in monoclonal antibody drugs was validated to study its applicability and transferability between various laboratories, and the feasibility to be included in the Chinese Pharmacopoeia. Both J.T. Baker and Nanjing Well-sourced polysorbate 80 was used in the collaborative validation of polysorbate 80 content analysis in seven different laboratories. The results show that when the protein concentration was no more than 20 mg·mL-1 and the concentration of polysorbate 80 ranged from 0.05 to 0.5 mg·mL-1, the method had good specificity. The recovery rates of the spiked samples ranged from 92.20% to 117.70% for J.T.Baker and from 93.90% to 117.20% for Nanjing Well. The intra-laboratory precision (%RSD) was less than 4.30% for J.T. Baker, and less than 2.60% for Nanjing Well, while the overall precision was less than 5.45% for J.T. Baker, and less than 6.70% for Nanjing Well. The linear correlation coefficient was more than 0.98 for J.T. Baker and more than 0.99 for Nanjing Well. The results of the collaborative validation prove that the HPLC-FMA method has good accuracy, precision, linearity, and specificity, and could be used for release control analysis of polysorbate 80 content in monoclonal antibodies across different laboratories.

6.
Article in Chinese | WPRIM | ID: wpr-882808

ABSTRACT

Objective:To investigate the incidence of asthma and the characteristics of acute attacks in children aged 3-14 in different administrative areas of Wuxi, and to analyze the correlation of asthma with the outdoor environmental factors.Methods:A total of 10 175 children aged 3-14 were randomly selected from 5 different administrative regions in Wuxi City, Jiangsu Province for childhood asthma epidemiological survey from January to December in 2017.Results:The complete questionnaires were collected from 8 318 children with a response rate of 81.70%.There were 6 734 valid questionnaires.Asthma occurred to 259 children, and the prevalence rate was 3.84%.The prevalence of asthma in male and female children was 4.89% (163 cases) and 2.82% (96 cases), respectively.The ratio of male patients to female patients was 1.70∶1.00, and the difference was significant ( χ2=19.72, P<0.01). Children were most prone to suffer from asthma attacks at the turn of seasons (87/259 cases, 33.59%). The frequency of asthma was the lowest from June to August (9/259 cases, 3.47%). Among 244 cases attacked by acute asthma, the prevailing time period of acute attacks was irregular in 88 patients (36.07%), bedtime in 64 cases (26.23%). In different regions of the city, the lowest prevalence rate of asthma was found in Binhu District (50/1 830 cases, 2.73%), the highest prevalence rate of asthma was found in Xinwu District (71/1 502 cases, 4.72%), the difference between the highest and lowest prevalence rates of asthma was significant ( χ2=13.19, P<0.05). In terms of the air quality in different admi-nistrative regions of Wuxi, the nitrogen dioxide (NO 2) concentration, the carbon monoxide (CO) concentration, PM 10 and PM 2.5 in Binhu District were lower than other 4 regions.In different seasons in Wuxi city, the sulfur dioxide(SO 2) concentration, the NO 2 concentration, the CO concentration, PM 10 and PM 2.5 from June to August were significantly lower than those in other months.The prevalence of asthma in children in different administrative districts of Wuxi was weakly positively correlated with the SO 2 concentration, the NO 2 concentration and PM 10 ( r=0.10, P<0.01; r=0.22, P<0.01; r=0.06, P<0.01, respectively). The prevalence of asthma was weakly negatively associated with the ozone(O 3) concentration ( r=-0.06, P<0.01). Acute asthma attacks were weakly positively correlated with the SO 2 concentration ( r=0.22, P<0.01), the NO 2 concentration ( r=0.28, P<0.01), the CO concentration ( r=0.23, P<0.01), PM 10 ( r=0.18, P<0.01) and PM 2.5 ( r=0.18, P<0.01), and weakly negatively correlated with the O 3 concentration ( r=-0.40, P<0.01). Conclusions:The prevalence of asthma in Wuxi is higher in boys than in girls, and the frequency of asthma attacks is related to the season.The SO 2, NO 2, CO, PM 10, PM 2.5 and O 3 concentration affect the prevalence and acute attacks of asthma.

7.
Article in Chinese | WPRIM | ID: wpr-864726

ABSTRACT

Objective:To understand the driving factors of postpartum health promotion behaviors in pregnant women with gestational diabetes.Methods:From September to October 2019, the purpose of the sampling method was to select 14 cases of gestational diabetic gynaecologists who visited the outpatient department of diabetes care in our hospital as the research subjects. The phenomenological research method was used to conduct a semi-structured interview. Colaizzi′s 7-step analysis method was used to organize and analyze the data to refine the subject.Results:The driving force of postpartum health promotion behaviors of gestational diabetes mothers mainly came from the internal driving force and the external driving force. The internal driving force was mainly the uncertainty of gestational diabetes, the solution of the problem improves self-worth, and the post-traumatic growth made patients re-understand life. The external driving force was mainly family support, full mobilization of subjective initiative, quantitative diet intake and building of a healthy lifestyle.Conclusions:The driving factors for postpartum health promotion of gestational diabetic women are complex and diverse. Medical staff should timely grasp the post-natal health promotion status of gestational diabetic women, and promote their self-support and social support.

8.
Article in Chinese | WPRIM | ID: wpr-905449

ABSTRACT

Objective:To investigate the changes of metabolite concentration in the precentral gyrus (primary motor cortex, M1) in spinal cord injury (SCI) patients. Methods:From December, 2018 to October, 2019, 20 SCI patients and 15 healthy controls were scaned with magnetic resonance spectroscopy to measure the concentrations of N-acetylaspartate (NAA), choline (Cho), creatine (Cr) and myo-inositol (MI) in region of interest (ROI) of left M1. Results:Concentration of MI was more in the patients than in the controls (t = 3.745, P < 0.01). There was no significant difference in the concentrations of NAA, Cho and Cr, as well as the ratios of NAA/Cr, Cho/Cr, Cho/NAA between the patients and the controls (t < 1.431, P > 0.05). Conclusion:There may be hyperplasia of glial cells in M1 of SCI patients, indicating compensatory repair in cerebral motor cortex.

9.
Chinese Journal of School Health ; (12): 1128-1130, 2020.
Article in Chinese | WPRIM | ID: wpr-825062

ABSTRACT

Objective@#To compare injuries prevalence among elementary and high school students in Beijing in the year of 2012 and 2017, and to provide a scientific basis for formulating the policies and measures on injuries prevention and control for the students in Beijing.@*Methods@#A multi-stage sectional random group sampling method had been used in the two surveys to sample from elementary and high school students in 16 districts of Beijing, and a retrospective questionnaire survey was conducted to investigate the injury occurrence during the past year, injury incidence in the year of 2012 and 2017 was compared.@*Results@#The incidence of injuries was 10.68% among elementary and high school students in 2016-2017 year period, which was lower than that in 2011-2012 year period(12.87%)(χ2=41.65,P<0.01). In 2016-2017 year period, the incidence of injuries for boys was 12.98%, and for girls was 8.63%, which were all lower than that in 2011-2012 year period(15.64% and 10.19%)(χ2=25.09, 13.57, P<0.01). Among all types of injuries, the incidence of falls was the highest in the two academic years. The incidence of falls in elementary and high school students in 2016-2017 year period was 6.35%, which was lower than that in 2011-2012 year period(7.36%)(χ2=14.68, P<0.01). The number of the injury cases being occurred in schools was at the top in the two academic years. The incidence rate of school injuries among students in 2016-2017 year period was 4.60%, which was lower than that in 2011-2012 year period (5.36%)(χ2=11.02, P<0.01). The incidence rates of unintentional injury, self-injury as well as intentional injury from otherrs in elementary and high school students in 2016-2017 year period were all lower than the rates in 2011-2012 year period(χ2=25.58, 10.05, 16.14, P<0.01).@*Conclusion@#After adopting a series of strategies and measures, student injuries decreased indicating that these measures are effective. In the future,more effective methods for student injury prevention should be developed to prevent and control the occurrence of the injuries for students.

10.
Chinese Journal of School Health ; (12): 965-966, 2020.
Article in Chinese | WPRIM | ID: wpr-823154

ABSTRACT

Abstract@#In order to prevent and control the occurrence of injuries in family settings among the elementary and high school students, information on injury prevention and control of the injuries in family settings was collected regarding injuries among the elementary and high school students together with serveillance data on student injuries in some of the elementary and high schools in Beijing. Also through searching the relevant literatures, the potential safety hazards and the key prevention and control linkages for the elementary and high school students in family settings had been pointed out through the five living scenes of living room, dining room, children’s room, kitchen and bathroom. This had guided the parents to create a good family life plus the learning environment. The behavioral skills of preventing and controlling the home injuries for the elementary and high school students had been proposed also. The guideline is proposed to prevent and control injuries in family settings for elementary and high school students too. Those can guide the parents and the students in their home life and learning period, or can be used by the Teachers and the Professionals to popularize and promote the applications in teaching and working aspects.

11.
International Eye Science ; (12): 837-839, 2019.
Article in Chinese | WPRIM | ID: wpr-735215

ABSTRACT

@#AIM: To observe the eye development and complications after primary intraocular lens implantation(IOL)in infantile cataract.<p>METHODS: The postoperative complications, corrected vision, refractive state, ocular axis of 46 patients(92 eyes)with congenital cataract treated by IOL implantation within 10y were retrospectively analyzed.<p>RESULTS: Five eyes(5%)had postoperative complications. In this study, the average corrected visual acuity was 0.40±0.10 at 3a follow-up after surgery, 0.51±0.01 at 5a follow-up after surgery, and 0.71±0.01 at 7a follow-up; The axial length of the eyes was 20.04±0.02mm at 3a after surgery, 22.09±0.09mm at 5a after surgery, and the axial length of the eyes was 22.96±0.06mm at 7a after surgery; The mean refractive state(equivalent spherical lens degree)at 3a after surgery was observed to be +5.37±0.12 DS, +3.20±0.20 DS at 5a after surgery, and +0.92±0.20 DS at 7a after surgery.<p>CONCLUSION: The appropriate surgical strategies can give safely and effectively opportunity for the patients with infantile cataract, which also have relatively normal refractive development.

12.
Article in Chinese | WPRIM | ID: wpr-837916

ABSTRACT

Objective To explore the mechanism of gastrointestinal dysfunction caused by chronic renal failure (CRF), and to determine whether colon is involved in the activation of oxidative stress (OS) in CRF. Methods Thirty male SD rats were randomly divided into control group (n=10) and CRF group (n=20). The rats in the CRF group were treated with 5/6 nephrectomy to establish CRF model, and the rats in the control group were only sutured after opening renal capsule. The rats were sacrificed at 10 weeks after model administration, and the serums and colon tissues near ileocecal valve were collected. Blood urea nitrogen (BUN) and serum creatinine (SCr) were measured to evaluate the success of the model. Malonodialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (TAC) and 8-hydroxy deoxyguanosine (8-OHdG) in the serum and colon tissues were detected to evaluate the level of OS. The ubiquinol cytochrome C reductase core protein(UQCRC1) was tested for the evaluation of mitochondrial function. Results Compared with the control group, the levels of BUN and SCr in serum of the rats in the CRF group were increased, suggesting that the model was successfully established. Compared with the control group, serum and colonic MDA levels were significantly increased in the CRF group (P0.05); however, there were no significant differences in 8-OHdG or anti-oxidative markers (SOD, TAC) in serum or colon tissues between the two groups (P0.05). The protein level of UQCRC1 in colon tissues was significantly reduced in the CRF group compared with the control group (P0.05). However, there was no significant difference in the mRNA level of UQCRC1 in colon tissues between the control and CRF groups (P0.05). Conclusion There is an imbalance between oxidation and antioxidation in the colonic tissues of CRF rats, which may be related to mitochondrial dysfunction.

13.
Article in Chinese | WPRIM | ID: wpr-776498

ABSTRACT

OBJECTIVE@#To investigate the therapeutic effects of puerarin on rats with type 2 diabetes mellitus (T2DM).@*METHODS@#T2DM models were established by high fat and high glucose feeding combined with a one-time intraperitoneal injection of streptozotocin (STZ, 60 mg/kg). Then the rats were randomly divided into normal group, model group, metformin group (MET, 40 mg/kg), puerarin low-dose group, medium-dose group and high-dose group (40, 80, 160 mg/kg), n=10. After the model was successfully established, rats were treated with corresponding drug intervention by intragastrical administration for 4 weeks. The body weight and fasting blood glucose (FBG) were measured per week, and blood samples were collected 24 h after the last administration, and serum levels of blood glucose, serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholestrol (HDL-C), serum enzyme activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), serum creatinine (SCr), and blood uric acid (UA) were measured.@*RESULTS@#As compared with normal group, the body weight was decreased after 4 weeks-intervention in the model group, and the levels of FBG, TC, TG, LDL-C, ALT, AST, BUN, SCr and UA were all increased,while HDL-C level was decreased (P<0.05). As compared with model group,the body weight was increased after 4 weeks-intervention in metformin group and puerarin groups, and the levels of FBG, TC, TG, LDL-C, ALT, AST, BUN, SCr and UA were decreased (P<0.01); meanwhile, HDL-C level was increased significantly (P<0.05).@*CONCLUSION@#Puerarin can reduce the weight loss of T2DM rats, decrease the blood lipid and blood glucose levels of T2DM rats, which can be used to control T2DM.


Subject(s)
Animals , Blood Glucose , Diabetes Mellitus, Experimental , Drug Therapy , Diabetes Mellitus, Type 2 , Drug Therapy , Isoflavones , Pharmacology , Lipids , Blood , Random Allocation , Rats , Streptozocin , Weight Loss
14.
Article in Chinese | WPRIM | ID: wpr-816125

ABSTRACT

OBJECTIVE: To investigate the clinical characteristics of patients with Fanconi syndrome(FS)sencondary to Sjogren's syndrome(SS). METHODS: The clinical data of 7 patients with FS secondary to SS were retrospectively analyzed. The clinical manifestations, auxiliary examinations, treatment options and curative effects were analyzed. RESULTS: Besides xerostomia and xerophthalmia, fatigue, polyuria and bone pain were found in 7 patients. Osteoporosis occurred in 6 cases and renal insufficiency in 3 cases. Immunoglobulin was increased in 7 cases, including 7 with IgG increase and 4 with IgG, IgA and IgM increase. All patients showed different degrees of ionic disorder and vitamin D deficiency. Renal glycosuria and amino acid urine were found in all 7 patients. All patients were treated with glucocorticoid combined with immunosuppressive agents. At the same time,they were treated with maintenance of acid-base and electrolyte balance and supplementation of active vitamin D. The curative effect was good. CONCLUSION: FS secondary to SS is rare, and patients are more prone to osteoporosis and renal insufficiency. Early diagnosis and treatment are essential.

15.
Article in Chinese | WPRIM | ID: wpr-703183

ABSTRACT

Objective To observe the changes of renal tubular injury and the extent of interstitial fibrosis in the C57BL/6 mouse models of chronic kidney disease(CKD),and provide experimental animal evidence for study of the pro-gression of acute kidney injury(AKI)to chronic kidney disease as well as its mechanisms. Methods Twenty-four 8-week-old male C57BL/6 mice were randomly and equally divided into control group, low-dose, medium-dose, and high-dose cisplatin groups,6 mice in each group. Mice in the cisplatin groups were administrated with 5,7 or 10 mg/kg cispla-tin by intraperitoneal injection once a week for 4 weeks. Plasma creatinine and 24-hour urinary protein were detected to as-sess the renal function. The mice were sacrificed, and plasma and kidney samples were collected for subsequent tests. Pathological changes were observed using periodic acid-Schiff(PAS)staining. To evaluate renal tubules injury, the ex-pression of kidney injury molecule 1(KIM-1)was examined by immunohistochemistry and the level of urinary N-Acetyl-β-D-glucosaminidase was detected with a commercial kit. The infiltration of CD3-positive T cells and F4/80-positive macro-phages was observed by immunohistochemistry(IHC)and immunofluorescence. The expression of collagen I and α-smooth muscle actin(α-SMA)were tested by immunohistochemistry to assess the renal fibrosis, while total kidney collagen was detected by Picrosirius red staining. Results In contrast to the normal control group,the kidney injury became more seri-ous in the cisplatin-treated mice as cisplatin concentration increased. Particularly,significant kidney damage was observed in the high-dose cisplatin group. Compared with the control group,the plasma creatinine and 24-hour urinary protein were significantly increased in the high-dose cisplatin group(P<0.05 and P<0.001)indicating impaired renal function. Mor-phologically,numerous clear vacuoles and necrosis were present in renal tubule epithelial cells in the high-dose cisplatin group. The expression of KIM-1 was markedly up-regulated and the level of urinary NAG was elevated. Infiltration of CD3-positive T cells and F4/80-positive macrophages was enhanced in the mice of high-dose cisplatin group. Data from immuno-histochemistry and picrosirius red staining showed that mice of the high-dose cisplatin group developed renal fibrosis evi-denced by markedly up-regulated expression of collagen I and α-SMA. Conclusions Repeated administration of 10 mg /kg cisplatin for 4 weeks can induce chronic renal insufficiency in mice,which may serve as a novel model for the research on underlying mechanisms of progression from acute kidney injury to chronic kidney disease.

16.
Chinese Journal of Immunology ; (12): 618-622, 2018.
Article in Chinese | WPRIM | ID: wpr-702785

ABSTRACT

Acute kidney injury (AKI) refers to the clinical syndrome of rapid loss of renal function caused by various causes.AKI increases the risk of developing chronic kidney disease (CKD) and the mortality of patients.The increase in the rate has caused a great economic burden on the family and society.The pathogenesis of mediating AKI is numerous,and it is currently believed that innate and adaptive immune-mediated inflammatory reactions are involved in the initiation,progression and repair stage of AKI.T lymphocytes play a key role in it.This article will review the progress of the role of T cells in AKI.

17.
Chinese Journal of Immunology ; (12): 127-131, 2018.
Article in Chinese | WPRIM | ID: wpr-702687

ABSTRACT

Systemic lupus erythematosus (SLE)is an autoimmune disease whose pathogenesis is extremely complicated.With the further research,the role of inflammasome in the pathogenesis of Lupus nephritis has also been gradually emphasized.Among them,the nucleotide-binding oligomerization domain-like receptors family pyrin domain containing 3 (NLRP3) inflammasome is the most exhaustive inflammasome.We summarize the related studies on the role of NLRP3 inflammasome in Lupus nephritis in recent years.We found that NLRP3 inflammasome not only plays an important role in the pathogenesis of Lupus nephritis,but also participates in the process of kidney injury by circulating immune cells and renal innate cells.Finally,we introduced two specific inhibitors of NLRP3 inflammasome,β-hydroxybutyrate and MCC950,which provided a new strategy for the treatment of Lupus nephritis.

18.
Article in Chinese | WPRIM | ID: wpr-702505

ABSTRACT

Neurogenic bowel dysfunction is one of the most common complications of spinal cord injury.It can cause abdominal distension,constipation,fecal incontinence,difficult defecation,prolonged defecation and other symptoms.At present, neurogenic bowel dysfunction is mainly evaluated through general condition,physical examination,experimental exami-nation,professional scales and intestinal microbiota,etc.

19.
Article in Chinese | WPRIM | ID: wpr-698232

ABSTRACT

Objective To investigate the possible molecular mechanism for alpha(α)-mangostin's inhibition of the proliferation and apoptosis of human gastric cancer cells.Methods Human gastric adenocarcinoma SGC7901 cell line was treated with α-mangostin.CCK8 method was used to measure the viability of SGC7901 cells.The effect of α-mangostin on apoptosis and cell cycle was determined by immune fluorescence and flow cytometry.The expression of the relevant proteins was detected using Western blot.The shapiro-wilk test was performed for evaluation of deviation from normality.Normally distributed data was analyzed with one-way ANOVA.Welch test was used in data with heterogeneity of variance and multiple compared by Games-Howell test after that.Results CCK8 results showed that cell viability differed significantly among groups treated with different concentrations of α-mangostin(10,15,20,25,and 30 μmol/L)(P<0.05).QPCR data showed that the concentration of α-mangostin was positively correlated with mRNA level of LC 3 but not caspase protein(r=0.976,P<0.05).In 15 μmol/L but not 10 μmol/L α-mangostin treatment system,the autophagy inhibitors 3-MA(10 μmol/L),bafilomycin A(10 μmol/L)and LY294002(10 μmol/L)could significantly alleviate α-mangostin's killing effect on SGC7901 cells(P<0.05).Conclusion The anti-tumor effects of α-mangostin against human gastric adenocarcinoma cells in vitro can be partly attributed to apoptosis,autophagy and arresting cell phase.

20.
International Eye Science ; (12): 1271-1274, 2018.
Article in Chinese | WPRIM | ID: wpr-695426

ABSTRACT

·AIM: To investigate the visual recovery factors in patients with complex traumatic and non-traumatic retinal detachment. ·METHODS: According to the history of ocular trauma before admission, 135 patients with complex retinal detachment were divided into traumatic group ( n=66, 66 eyes) and non-traumatic group (n=69, 69 eyes). The results of visual recovery and complications were compared between the two groups. Logistic regression was used to analyze the related factors of visual recovery in the two groups. ·RESULTS: There was no significant difference in the recovery rate between the two groups within 24h after surgery (77. 3% vs 78. 3%, P>0. 05), and there was no significant difference in the total incidence of complications at 3mo after surgery(18. 2% vs 17. 4%, P>0. 05). Multifactorial Logistic regression analysis showed significant correlation between age, injury type, time since retinal detachment, rage of retinal detachment, preoperative vitreous blood,proliferative vitreoretinopathy ( PVR ) and postoperative visual recovery in patients with complex traumatic retinal detachment ( P < 0. 05 ); age, time since retinal detachment, rage of retinal detachment and macular status were significantly associated with visual recovery in patients with complex non-traumatic retinal detachment (P<0. 05). ·CONCLUSION: Age, time since retinal detachment and rage of retinal detachment were significantly associated with traumatic and non-traumatic retinal detachment. The injury type, preoperative vitreous hemorrhage, PVR were significantly correlated with the visual recovery of traumatic retinal detachment patients. The condition of macular was significantly associated with the visual recovery of non-traumatic retinal detachment patients.

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