ABSTRACT
Objective@#To detect the single nucleotide polymorphism (SNP) at locus 1 165 of β1-adrenoceptor (β1-AR) and to investigate the association between the SNP and the infection by enterovirus A71(EV-A71).@*Methods@#Polymerase chain reaction (PCR) amplification technique was used to detect the SNP at locus 1 165 of β1-AR between hand, foot and mouth disease (HFMD) and healthy controls by sanger sequencing method .@*Results@#There was a G1165C SNP and three kinds of genotypes (GG, GC, CC) in β1-AR gene in the 77 cases of EV-A71 HFMD patients and 66 cases of healthy controls. For HFMD patients, frequencies of GG, GC and CC genotypes of the G1165C locus were 10%, 47% and 43%, respectively, and alleles frequency of G and C were 34% and 66%, respectively. But in healthy children, GG, GC, CC genotype frequencies were 7%, 41% and 52%, respectively, and G and C allele frequencies were 28% and 72% respectively. Chi-square analysis showed that there were no significant differences in distribution of genotypes (χ2=1.154, df=2, P=0.562) and alleles frequency (χ2=1.091, df=2, P=0.296) between the EV-A71-infected group and the healthy control group. Between mild and severe EV-A71-infected group, there were no significant differences in distribution of genotypes (χ2=3.945, df=2, P=0.139) and alleles frequency (χ2=3.763, df=2, P=0.052).@*Conclusions@#The 1 165 SNP in the coding region of β1-AR was not associated with EV-A71 infection and its severity.
ABSTRACT
Objective To investigate the influence of autoantibodies against the second extracellular loop of β1-adrenoceptor ( β1-AA) on the proliferation ability of LPS-stimulated rat B lymphocytes.Methods Active immunization assay was used to obtain adequate IgGs in which β1-AA was positive; MACS (magnetic activated cell sorting) assay was used for gaining rat splenic B lymphocytes; CCK8 assay was used for detecting the influence of β1-AA to the proliferation abilities of silent and LPS-stimulated rat splenic B lymphocytes.Results β1-AA (0.1 μmol/L pIgGs ) promoted the proliferation of LPS-stimulated rat splenic B lymphocytes(A values:0.739±0.036 vs 0.533±0.032,P＜0.05),and the effect was likely to be concentration-dependent.And the effect could be blocked by β1-AR blocker or β2-AR blocker partially,and could be blocked by β1-AR blocker and β2-AR blocker completely; β1-AA had no proliferation effect on silent rat splenic B lymphocytes.Conclusion β1-AA could promote the proliferation of LPS-stimulated rat splenic B lymphocytes via β1-AR and β2-AR which were on the surface of B lymphocytes.Thus,it could provide new clues for complex pathologic mechanism of cardiovascular patients in which β1-AA is positive.