ABSTRACT
OBJECTIVE: To study the anti-inflammatory active constituents of Mangifera indica L. seed kernel. METHODS: The compounds were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography and ODS column chromatography.Their structures were elucidated by spectral analyses and physicochemical properties. The anti-inflammatory activities of selected isolated compounds were evaluated as inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cell lines by Griess reaction. RESULTS: Eight compounds were obtained from n-butanol fraction oi Mangifera indica L. seed kernel, whose structures were elucidated as 4-O-ethylgallic acid (1), gallic acid (2), citric acid(3), 1,2,3,4,6-penta-O-galloylglucose(4),1,3, 6-tri-O-galloylglucose(5), hyperoside(6), quercetin-3-O-rhamnopyranoside(7), and mangiferin(8). Compounds 1, 2 and 4-8 exhibited potent inhibitory effect on NO production in LPS-induced macrophages, especially compounds 6 and 8 showed the best inhibitory activity with IC50 values of 16.5 and 19.5 μmol·L-1, respectively. CONCLUSION: Compounds 1 and 3-8 were obtained from Mangifera indica L.seed kernel for the first time.Compounds 1, 2 and 4-8 are the anti-inflammatory active constituents oi Mangifera indica L.seed kernel.Compounds 6 and 8 are the most potent anti-inflammatory active constituents.
ABSTRACT
Objective: To study the chemical constituents of mango (Mangifera indica) seed kernel and their effects on sperm quality of asthenospermia rats in vitro. Methods: The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography, and recrystallization. Their structures were elucidated by spectral analyses such as 1H-NMR, 13C-NMR, and MS. The rats were treated with a single ig administration tripterygium glycosides to induce asthenospermia rat model, so that the effects of the compounds could be evaluated on the sperm motility and sperm survival rate of asthenospermia rats in vitro. Results: Nine compounds were isolated and purified from mango seed kernel, whose structures were elucidated as vitamin E (1), methyl gallate (2), kaempferol (3), quercetin (4), 3,4-O-isopropylidene shikimic acid (5), 5-hydroxy-4-oxopyran-2-carboxylic acid (6), ferulic acid (7), isoquercitrin (8), and 1,2,3,4,6-penta-O-galloylglucose (9). Compounds 1, 7, and 9 could increase the sperm motility and sperm survival rate of asthenospermia rats in vitro. Conclusion: Compounds 1, 3-6 and 8 are obtained from mango seed kernel for the first time; Compounds 1, 7, and 9 could improve the sperm quality of asthenospermia rats in vitro.