ABSTRACT
Objective: To study the chemical constituents of the herbs of Patrina scabiosaefolia. Methods: Many column chromatographies such as silica gel, Sephadex LH-20, ODS column chromatography and preparative high-performance liquid chromatography were used to separate and purify the chemical constituents of P. scabiosaefolia. The structures of these compounds were identified by physical-chemical data and spectroscopic techniques. Results: Eighteen compounds were isolated and purified from the ethanol extract of P. scabiosaefolia, and their structures were identified as follows: 6-methoxy-7-(1’-henicosacyloxy- 3’-hydroxy-2’-propoxy) coumarin (1), oleanolic acid (2), ursolic acid (3), palmitic acid (4), dodecanoic acid (5), α-tocopherolquinone (6), maslinic acid (7), 4-hydroxy-3-methoxybenzaldehyde (8), 2,6-dimethoxy benzoquinone (9), 3β-acetyloxy-12-en-28-ursolic acid (10), α-tocospiro B (11), 22E-3β-hydroxy-5α,6α-epoxyergosta-22-en-7-one (12), 2α-hydroxy ursolic acid (13), linoleic acid (14), β- sitosterol palmitate (15), 3-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-1-propanone (16), burselignan (17), and hederagenin (18). Conclusion: Compound 1 is a new compound named scabiosaelactone and compounds 6, 8, 9, 11, 12, 16, and 17 are isolated from the genus Patrina for the first time.
ABSTRACT
Objective: To investigate the chemical constituents in the roots of Ilex kudingcha. Methods: Column chromatography such as silica gel, Sephadex LH-20, and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS, 1H-NMR, and 13C-NMR, and physical constants were used to elucidate their structures. Results: Nineteen compounds were isolated from 95% ethanol extracts of I. kudingcha, including α-aromadendrol-3β-palmitate (1), β-sitosterol (2), α-amyrin (3), 3-acetoxyolean-12-en-28-ol (4), 3-acetoxyurs-12-en-28-ol (5), 3β-acetoxyurs-12-en-28-al (6), oleanolic acid (7), ursolic acid (8), quercetin (9), daucosterol (10), ficuscarpanoside B (11), 3β-hydroxy-28-norurs-12, 17-dien (12), siaresinolic acid (13), pomolic acid (14), α-D-glucose (15), 2, 6-dimethoxy-benzoquinone (16), syringaldehyde (17), dibutyl phthalate (18), and ilexoside D (19). Conclusion: Compounds 6, 12, 16-18 are isolated from the plant for the first time. Compound 11 is isolated from Ilex L. for the first time.