ABSTRACT
Fundus photography is an arduous task as it involves using 90 D in one hand and a smartphone attached on an eyepiece of a slit-lamp biomicroscope in the other hand. Similarly, with a 20 D lens, the filming distance is adjusted by moving the lens or mobile forward or backward, which makes it difficult to adjust and focus the image in busy ophthalmology outpatient departments (OPDs). Moreover, fundus camera costs thousands of dollars. Authors describe a novel technique of performing fundus photography with a 20 D lens and a universal slit-lamp–mounted mobile adapter made from trash. By the use of this simple, yet frugal innovation, primary care physicians or ophthalmologists without a fundus camera can easily snap a fundus photo and subject it to digital analysis by retina specialists across the world. This will help in simultaneous ocular examination and fundus photos taken via mounted 20 D on a slit lamp itself and also reduce the need for unnecessary retina referrals to tertiary eye care centers.
ABSTRACT
@#[摘 要] 目的：探讨肽酶M20结构域1（peptidase M20 domain containing 1，PM20D1）在人弥漫大B细胞淋巴瘤（diffuse large B-cell lymphomas，DLBCL）细胞中的表达及其与缺氧相关性和预后的关系。方法：通过GDC、TCGA、GTEx公共数据库分析PM20D1表达对DLBCL细胞增殖、迁移和凋亡的影响及其与患者预后的关系。通过对不同分组患者的富集分析及PM20D1与CD274相关性分析验证PM20D1是否为DLBCL的缺氧相关基因；采用ChEA、ENCODE和hTFtarget数据库分析上游调控PM20D1表达的转录因子（TF）和miRNA，以及差异表达PM20D1与化疗药物敏感性的关系。采用WB法检测PM20D1在正常淋巴细胞和DLBCL细胞中的表达水平，通过设计PM20D1的siRNA序列敲减目的基因，并采用qPCR和WB法检测验证SUDHL2和SUDHL10细胞中PM20D1的敲减效率，采用CCK-8法和Transwell实验分别检测敲减PM20D1对细胞增殖和迁移能力的影响，流式细胞术检测细胞凋亡水平。结果：PM20D1在DLBCL组织中高表达且患者预后差（P<0.05或P<0.01）；富集分析显示，PM20D1高表达组与ssGSEA高分组主要涉及细胞电耦合通讯、甘油三酯代谢过程调节和细胞质翻译起始复合物过程，且PM20D1表达与免疫检查点CD274表达呈正相关（P<0.01，r=0.757）。在SUDHL2和SUDHL10细胞中敲减PM20D1后，细胞的增殖和迁移均显著降低（均P<0.05），细胞凋亡明显增加（P<0.05）。结论：PM20D1基因在DLBCL组织和细胞中高表达且与患者预后密切相关；PM20D1可能通过促进DLBCL细胞的增殖、迁移并抑制凋亡，从而促进DLBCL的发生发展。