ABSTRACT
OBJECTIVE: To establish a simple and sensitive method for simultaneous detection of chlorpyrifos( CPF),and its metabolite 3,5,6-trichloro-2-pyridinol( TCP) in plasma samples by ultra performance liquid chromatography( UPLC).METHODS: The 0. 5 m L of blood sample was extracted by ethyl acetate,then separated by C18 column using acetonitrile /water as a mobile phase and detected by diode-array detector under the ultraviolet spectrum of 298 nm( 0. 0-2. 0 min) and289 nm( 2. 0-5. 0 min) through UPLC. RESULTS: The method showed that a good linear range was 1. 00-50. 00 mg / L of both CPF and TCP,and the correlation coefficients was 0. 999 9. The limit of detection was 0. 30 mg / L and the lower limits of quantitation was 1. 00 mg / L of both CPF and TCP. The recovery rates of CPF and TCP were 87. 20 %-103. 08 %and 86. 20 %-99. 28 %,respectively. The within-run relative standard deviation( RSD) of CPF and TCP was 5. 28 %-6. 17 % and 2. 32 %-4. 43 %,and the between-run RSD was 6. 62 %-7. 53 % and 3. 55 %-5. 24 %. Samples can be kept in4 degrees Celsius refrigerator for 7 days. CONCLUSION:s The proposed method is simple,reliable and sensitive. It can be applied for simultaneous detection of CPF and its metabolite TCP in plasma samples.
ABSTRACT
<p><b>OBJECTS</b>We carried out an investigation to clarify the real state of indoor air pollution by chlorpyrifos (termiticide) and exposure to chlorpyrifos of residents by measuring its urinary metabolite 3,5,6-trichloro-2-pyridinol (TCP) as an exposure index, such as biological monitoring.</p><p><b>METHODS</b>The investigation was conducted in 43 individual houses with termiticide application (whether the termiticide was chlorpyrifos is uncertain) and 3 control houses without any termiticide application in Kagawa, Japan. Urine samples were collected from 46 healthy adult residents of the aforementioned houses.</p><p><b>RESULTS</b>Chlorpyrifos in indoor air in the control houses was not detected (ND<1 ng/m(3), n=3), while 41 of 43 houses with termiticide application showed 1-350 ng/m(3). Although the chlorpyrifos concentrations in these 41 houses did not exceeded the indoor air quality guideline of 1000 ng/m(3), but 3 houses were higher than the guideline 100 ng/m(3) for children in Japan. Urinary TCP concentrations of 0.1-7.8 ng/mg·creatinine were detected in 41 residents from the 41 houses where chlorpyrifos had been detected. The chlorpyrifos concentration and the urinary TCP revealed a positive correlation (r=0.5468, p<0.01, n=41).</p><p><b>CONCLUSIONS</b>The immediate health hazard from air born chlorpyrifos in the examined houses was negligible, but the findings suggest that it is necessary to monitor chemicals which may contaminate indoor air and to assess the risk of prolonged exposure to such chemicals. The measuring of urinary metabolite TCP of chlorpyrifos via biological monitoring would be useful, allowing comprehensive evaluation of the exposure to chlorpyrifos in indoor air.</p>