ABSTRACT
Objective: This study is aimed at developing an UPLC method for simultaneous determination of 1, 3-O-dicaffeoylquinic acid, 1,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, and 5-O-caffeoylquinic acid and fingerprint analysis of Inula cappa. Methods: WATERS ACQUITY UPLC BEH C18 column with gradient elution of 0.1% acetic acid-acetonitrile at a flow rate of 0.4 mL/min was employed for analysis of 20 batches of samples from three provinces or autonomous region. The detected wavelength was set at 329 nm. The column temperature was maintained at 30℃ and the sample solutions were maintained at 4℃ before analysis. Results: Twenty peaks were selected as the common peaks in fingerprint and the similarity of samples was above 0.900 except S18. The variance analysis, hierarchical clustering analysis, and principle component analysis were employed for the quality evaluation based on the contents of seven components and fingerprint similarity. The results indicated that the contents of 1,3- and 1,5-O-dicaffeoylquinic acid were significantly different among three provinces or autonomous region. The clustering analysis results showed that 20 batches of samples were divided into two classes and the quality of samples from Guangxi provinves were more stable than those from Guizhou and Yunnan. Conclusion: The established method could be rapid and accurate to evaluate the quality of I. cappa.
ABSTRACT
Objective: To establish the specific chromatogram of Yinhuang Drop Pills (YDP) by High performance liquid chromatography. Methods: The chromatographic column Kromasil 100-5C18 (250 mm × 4.6 mm, 5 μm) was used, acetonitrile-0.4% phosphoric acid as mobile phase with gradient elution, and the detection wavelength was 372 nm. Through similarity evaluation software, the similarity of specific chromatogram of 10 batches YDP were calculated. Results: The HPLC specific chromatogram of YDP showed 7 common peaks, of which 6 peaks (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid) from Lonicerae Flos, 1 common peaks (baicalin) from Scutellariae Radix; By comparing with the reference substances, identified 7 components, respectively, the neochlorogenic acid (peak 1), chlorogenic acid (peak 2), cryptochlorogenic acid (peak 3), 3,4-O-dicaffeoylquinic acid (peak 4), 3,5-O-dicaffeoylquinic acid (peak 5), 4,5-O-dicaffeoylquinic acid (peak 6), baicalin (peak 7). By the methodology validation, verified that this method has good precision, repeatability and stability, 10 batches of sample similarity is greater than 0.9. Conclusion: This method was available for the quality control of YDP.
ABSTRACT
CONCLUSION: The established HPLC method is simple, rapid and accurate, and can be used to control the quality of Valeriana jatamansi.