Correlation between hand-arm vibration disease and vasoactive factors / 环境与职业医学

Background Hand-arm vibration disease (HAVD) is a chronic progressive disease caused by long-term exposure to hand-transmitted vibration, but the mechanism by which vibration affects peripheral vascular function of fingers is not completely clear. Objective To study the association between vasoactive factors and HAVD, and to screen specific indicators for its early diagnosis and prevention. Methods Judgmental sampling method was used to select workers with (HAVD group) and without HAVD (vibration contact group), and non-hand-transmitted vibration operation workers (control group), with 60 workers in each group. The levels of leukotriene B4 (LTB4), vascular endothelial growth factor (VEGF), 5-hydroxy tryptamine (5-HT), interleukin-1β (IL-1β), and calcitonin gene-related peptide (CGRP) in plasma of the three groups were measured by enzyme-linked immunosorbent assay. The association between vasoactive factors and HAVD was analyzed using logistic regression, and the diagnostic HAVD indicators were screened by receiver operator characteristic (ROC) curve of a multivariate model indicator \begin{document}$ \widehat{Y} $\end{document}. Results The hand symptom rates of the HAVD group, the vibration contact group, and the control group were 26.7%, 66.7%, and 96.7% respectively, with a significant difference (P<0.05). The levels of LTB4, 5-HT, IL-1β, and CGRP in the HAVD group were the highest followed by the vibration contact group, and lowest levels were in the control group (P<0.05). There was no significant difference in the VEGF level among the three groups (P>0.05). The logistic regression results showed that higher levels of LTB4 (OR=1.048, 95%CI: 1.022-1.076), 5-HT (OR=1.011, 95%CI: 1.004-1.018), IL-1β (OR=1.148, 95%CI: 1.071-1.230), and CGRP (OR=1.055, 95%CI: 1.008-1.104) were associated with a higher risk of HAVD (P<0.05). The order of the potential indicators' area under the ROC curve from high to low was:\begin{document}$ \widehat{Y} $\end{document} (0.969) > IL-1β (0.907) > LTB4 (0.876) > 5-HT (0.858) > CGRP (0.836). Conclusion The expression levels of LTB4, 5-HT, IL-1β, and CGRP are altered with occupational exposure in hand-transmitted vibration operations and may be associated with HAVD; VEGF is not found to be associated with HAVD. The accuracy of early screening for HAVD can be improved by combining the monitoring of various biochemical indicators.

The effect of "paraplegic triple needling" combined with rehabilitation training on psychological and daily living ability of patients with spinal cord injury / 中国针灸

<p><b>OBJECTIVE</b>To compare the effects of "paraplegic triple needling" and conventional needling on psychological and daily living ability of patients with spinal cord injury.</p><p><b>METHODS</b>A total of 50 patients with spinal cord injury were randomized into an observation group and a control group, 25 cases in each one. Rehabilitation training was applied in the two groups. In the observation group, on the basis of rehabilitation training, "paraplegic triple needling" was added, namely, puncturing the governor vessel (GV) and back- points respectively in the upper and lower segments of the injury plane and locating the key muscle movement points of the lower extremities by the peripheral nerve electrical stimulation device. Electroacupuncture (EA) was given at the points up and down the two sections of the GV points, back- points, the key muscle movement points. In the control group, conventional needling was applied at the points of GV, back- points, Huantiao (GB 30), Zusanli (ST 36), Xuanzhong (GB 39) and Yanglingquan (GB 34). The treatment was given once a day, the treatment for a month as one course and a total of 2 course were required. In addition, 25 health checkers were selected at the physical examination center of General Hospital of Chengdu Military Region as a normal control group. The content of peripheral serum 5-hydroxy tryptamine (5-HT) was tested before treatment, 1 course and 2 courses of treatment, The modified Barthel index (MBI) was used to observe the daily living activities, the Hamilton depression scale (HAMD) and Hamilton anxiety scale (HAMA) were used to evaluate the psychological status.</p><p><b>RESULTS</b>In the two groups, the content of serum 5-HT was lower than that in the normal control group before treatment (both <0.05). After one course of treatment, the MBI score and content of serum 5-HT were all increase in the two groups; the HAMA score and HAMD score were all reduced (all <0.05); the results in the observation were better than those in the control group (all <0.05). After 2 courses of treatment, the MBI score and content of serum 5-HT were all increased in the two groups, and the HAMA score and HAMD score were all reduced (all <0.05), but the difference was not significant statistically between the two groups (all >0.05). Pearson correlation analysis showed that the content of serum 5-HT in patients with spinal cord injury was negatively correlated with disease course, HAMA and HAMD score (all <0.05), and positively correlated with MBI score (<0.05).</p><p><b>CONCLUSION</b>There are differences in the content of serum 5-HT between the normal person and the patients with pinal cord injury. The content of serum 5-HT can early predict the depression and anxiety in patients with spinal cord injury. Compared with the conventional needling, "paraplegic triple needling" can improve depression and anxiety in the early stage and improve the daily living ability of patients with spinal cord injury.</p>

Effect of auricular intradermal needling combined with erjian (HX) bloodletting on sleep quality and neuroendocrine level in patients with perimenopausal insomnia / 中国针灸

<p><b>OBJECTIVE</b>To compare the effect differences between auricular intradermal needling combined with erjian (HX) bloodletting and oral administration of western medicine, and to explore the efficacy of neuroendocrine level in patients with perimenopausal insomnia.</p><p><b>METHODS</b>Ninety patients were randomized into an observation group and a control group, 45 cases in each one. In the observation group, auricular intradermal needling combined with erjian (HX) bloodletting were adopted alternately in the two ears. The auricular points were shen (CO), xin (CO), gan (CO), shenmen (TF), jiaogan (AH), neifenmi (CO) and erjian (HX). The treatment was required once 3 days on the auricular points of one side alternatively. Oral administration of estazolam (1mg each day) was applied in the control group for 2 courses, 4 weeks as 1 course, once a day. The scores of Pittsburgh sleep quality index (PSQI), the levels of serum estrogen (E), 5-hydroxy tryptamine (5-HT) and norepinephrine (NE) were valuated in the two groups before and after treatment.</p><p><b>RESULTS</b>After treatment, the total scores of PSQI reduced in the two groups (both <0.05), and the improvements of sleeping quality, sleeping time, sleeping difficulty, daytime dysfunction and the total PSQI score in the observation group were superior to those in the control group (all <0.05). There was no significant difference in serum E before and after treatment in the two groups，and between the two groups after treatment (all >0.05). After treatment, 5-HT contents increased and NE levels decreased in the two groups (all <0.05), with better results in the observation group (both <0.05). The total effective rate was 95.6% (43/45) in the observation group, which was higher than 82.2% (37/45) in the control group (<0.05).</p><p><b>CONCLUSION</b>Auricular intradermal needling combined with erjian (HX) bloodletting can improve the sleep quality of patients with perimenopausal insomnia, and adjust the neurotransmitter level, which achieves better effect than western medication.</p>

Effects of Acupuncture on 5-HT, NE and DA in Serum of Insomnia Rats / 中国中医药信息杂志

Objective To observe the effects of acupuncture in acupoints at different meridians on 5-HT, DA, NE in the peripheral serum of insomnia rats; To explore the mechanism of action of acupuncture for the treatment of insomnia. Methods Sixty SD rats were randomly divided into blank group, model group, Baihui+Shenmen group, Baihui+Sanyinjiao group, and Baihui+non-acupoint group, with 12 rats in each group. Injection of PCPA suspension of rats was used to establish insomnia model. Each acupuncture group received corresponding acupoints, 30 min each time, for 7 days. The levels of 5-HT, DA and NE in serum were measured by ELISA. Results Compared with the blank group, the levels of 5-HT, DA and NE in the model group were significantly higher than those in the blank group (P<0.01). Compared with the model group, the contents of DA and NE in the acupuncture groups were significantly lower than those in the model group (P<0.05, P<0.01), and the 5-HT in Baihui+Sanyinjiao group was significantly lower than those in the model group (P<0.05). Among all acupuncture groups, overall efficacy of Baihui+Shenmen group was better than Baihui+Sanyinjiao group and Baihui+non-acupoint group. Conclusion Acupuncture can improve sleep structure in rats may be related to serum monoamine neurotransmitters. Acupuncture in acupoints at different meridians may be one of the influencing factors of acupoint compatibility effects.

Tryptophan metabolism change in patients with chronic hepatitis B and hepatitis B cirrhosis and its correlation analysis / 重庆医学

Objective To investigate the tryptophan (Trp) metabolism change in the patients with chronic hepatitis B (CHB) and hepatitis B cirrhosis (HBC) and to analyze the related risk .Methods Eighty-one cases of CHB and 40 cases of HBC hospitalized in the First Affiliated Hospital of Chongqing Medical University from October 2015 to January 2016 served as the stud-y subjects ,and 40 healthy persons as the controls .High performance liquid chromatography -fluorescence detection (HPLC-FD) were adopted to detect the levels of plasma Trp ,kynurenine (Kyp) and its metabolite 5-hydroxy tryptamine (5-HT) .HBV-DNA vi-ral replication number was detected by real-time quantitative PCR .The serological indicators differences among 3 groups were ana-lyzed .The risk factors were analyzed and screened by the Logistic regression analysis and Pearson correlation analysis .Results Compared with the control group ,plasma 5-HT and Trp levels in the CHB group and HBC group were significantly decreased (P<0 .01);plasma Kyn/Trp in the HBC group was significantly higher than that in the other two groups (P<0 .01) .Kyn/Trp in the HBC group was negatively correlated with A/G (r= -0 .686 ,P<0 .01) ,and Kyn/Trp in the CHB group was positively correlated with Log DNA (r= 0 .784 ,P<0 .01) .The Logistic regression analysis indicated that Kyn/Trp was the risk factor of CHB and HBC .Conclusion Detecting plasma Trp and its metabolite can serve as the adjuvant indicators for assessing the progression of hep-atitis B .

Effect of Polymorphisms of Three Genes Mediating Monoamine Signalling on Brain Morphometry in Schizophrenia and Healthy Subjects

OBJECTIVE: We examined the effect of risk alleles of polymorphisms of three schizophrenia risk genes that mediate monoamine signalling in the brain on regional brain volumes of schizophrenia and healthy control subjects. The risk alleles and the gene polymorphisms studied were: Val allele of catechol o-methyltransferase (COMT) rs4680 polymorphism; short allele of 5-hydroxy tryptamine transporter linked polymorphic region (5HTTLPR) polymorphism; and T allele of 5-hydroxy tryptamine 2A (5HT2A) rs6314 polymorphism. METHODS: The study was carried out on patients with recent onset schizophrenia (n=41) recruited from the outpatient department of National Institute of Mental Health and Neurosciences, Bangalore, India and healthy control subjects (n=39), belonging to South Indian Dravidian ethnicity. Individual and additive effects of risk alleles of the above gene polymorphisms on brain morphometry were explored using voxel-based morphometry. RESULTS: Irrespective of phenotypes, individuals with the risk allele T of the rs6314 polymorphism of 5HT2A gene showed greater (at cluster-extent equivalent to family wise error-correction [FWEc] p<0.05) regional brain volumes in the left inferior temporal and left inferior occipital gyri. Those with the risk alleles of the other two polymorphisms showed a trend (at p<0.001, uncorrected) towards lower regional brain volumes. A trend (at p<0.001, uncorrected) towards additive effects of the above 3 risk alleles (subjects with 2 or 3 risk alleles vs. those with 1 or no risk alleles) on brain morphology was also noted. CONCLUSION: The findings of the present study have implications in understanding the role of individual and additive effects of genetic variants in mediating regional brain morphometry in health and disease.

The concentrition change of serum 5-Hydroxy tryptamine and clinical symptoms improvement in primary premature ejaculation with the treatment of paroxitine / 国际检验医学杂志

Objective To investigate the relationship between concentration change of serum 5‐hydroxy tryptamine and clinical symptoms improvement in primary premature ejaculation with the treatment of paroxitine .Methods 81 cases of lifelong PE and an intra‐vaginal ejaculation latency time (IELT ) ≤60 s were included in this study .Subjects were divided into 2 groups according to the IELT ,group A (IELT≤30 s) and group B (30 s

Effects of timeshare administration of Jiaweisinisan on biological rhythm of rats with chronic stress / 中国药理学通报

Aim To study the effects of timeshare ad-ministration of Jiaweisinisan ( JWSNS ) on biological rhythm of rats with chronic stress. Methods Unpre-dictable mild chronic stress was used. Cosine rhythm analysis was adopted to observe and analyze the chan-ges of circadian rhythms of body temperature and se-cretion of 5-hydroxy tryptamine ( 5-HT ) , melatonin ( MT) and corticosterone ( CORT ) and the effects of JWSNS. Serum 5-HT,MT and CORT were detected by enzyme-linked immunosorbent assay. Results Three groups of administration of JWSNS had no inhibitory effects on weight gain in model rats,but improved the sucrose preference ( P Administration at 17 : 30 had biphasic regulatory effects on the peak and valley value,while administra-tion at 7 : 30 and 17 : 30 respectively mainly acted on the light phase,namely regulated the valley value. Rats with chronic stress showed delays of body temperature rhythm,namely postponed phase, which could only be inhibited by administration at 7 : 30 . There were de-creases in votatility and disorders in secretion rhythm, as was shown by dramatically accelerated phase of ser-um 5-HT, MT and CORT in rats with chronic stress. Administration of JWSNS at 17 : 30 or at 7 : 30 and 17 : 30 respectively had inhibitory effects on lower ser-um level of 5-HT in model rats ( P increase and the decreases in volatility. ConclusionAdministration of JWSNS at 7 : 30 achieves anti-de-pressant effects by regulating rhythms of hormones and neurotransmitters. Administration of JWSNS at 17 : 30 achieves anti-depressant effects by adjusting the secre-tion of hormones and neurotransmitters,while adminis- tration of JWSNS at 7 : 30 and 17 : 30 respectively a-chieves anti-depressant effects by those two above-men-tioned aspects.

Association among leptin, 5-HT and premature ejaculation / 中华内分泌外科杂志

Objective To investigate the interrelationship between leptin and 5-hydroxy tryptamine (5-HT)and their significance in diagnosis of premature ejaculation(PE).Methods 71 cases with lifelong PE(the trial group)and 64 healthy males(the control group)were enrolled in this case-control study.Baseline recording of intravaginal ejaculation latency time(IELT) using a stop watch and Chinese Index of Premature Ejaculation-5 (CIPE-5) were done.The serum leptin and 5-HT levels were determined.Then the trial group were given sertraline hydrochloride for 8 weeks,while the control group were not given any medicine treatment in this period.Reassessment was done after 8 weeks.Results At the baseline,the leptin level in the trial group was obviously higher than the control group(P ＜ 0.05),while 5-HT level in the trial group was far lower than the control group(P ＜0.05).After 8 weeks,the leptin level of the trial group dropped and the difference had no statistical significance with that of the control group(P ＞0.05).5-HT level of the trial group rose to the level very close to that of the control group after the treatment.Conclusion The high level of serum leptin and low level of serum 5-HT were the meaningful biological indicators for PE,which can potentially be applied in PE diagnosis.

Effect of Shenlongjiannaotang on Neurotransmitters in D-Glalactose Induced Aging Mimetic Rats / 中国康复理论与实践

@#Objective To investigate the influence of Shenlongjiannaotang on the function of learning and memory of D-glalactose induced aging mimetic rats,and the concentrations of norepinephrine(NE),dopamine(DA) and 5-hydroxy tryptamine(5-HT) in cerebrum.Methods 50 rats were randomly divided into 5 groups: normal control group,model group,and high/middle/low dosage prescription group of Shenlongjiannaotang.The memory function was tested with Morris water maze.The concentrations of NE,DA and 5-HT were detected with HPLC with fluorescence spetrometer.Results In the model group,the latency of model mice increased,and the correct number decreased significantly compared with normal group(P<0.05).After 6 weeks administering Shenlongjiannaotang,the latency decreased and the correct number increased significantly in high dosage group,compared with those of the model group(P<0.05).The contents of NE and DA reduced in model group,and Shenlongjiannaotang could increase NE and DA contents of rats cerebrum(P<0.05).Conclusion Shenlongjiannaotang could improve the learning and memory function of D-galactose induced aging rats,which may related with the increase of NE and DA contents in cerebral tissue.The effects depend on the dosage of Shenlongjiannaotang.