Analysis of 134Cs, 137Cs, and 60Co in aerosol samples: An intercomparison among laboratories / 中国辐射卫生

Objective To improve the analysis of 134Cs, 137Cs, and 60Co in aerosol samples by the national key radiation environment laboratories. Methods Intercomparison of analysis results of 134Cs, 137Cs, and 60Co in standard aerosol samples was performed among the national key radiation environment laboratories according to Gamma spectrometry method of analyzing radionuclides in biological samples (GB/T 16145-1995 ), and the intercomparison results were evaluated by the standard deviation. Results Six laboratories were involved in the intercomparison. For 134Cs, 50% of the laboratories showed a relative deviation less than 10%, and 50% showed a relative deviation of 10%-20%. For 137Cs, 33.3% of the laboratories showed a relative deviation less than 10%, and 76.7% showed a relative deviation of 10%-20%. For 60Cs, all laboratories showed a relative deviation less than 10%. The overall intercomparison results were acceptable. Conclusion The laboratories in this intercomparison show generally good results.

Role of complement in radiation-induced lung injury / 中国辐射卫生

@#<b>Objective</b> To investigate the role of complement in radiation-induced lung injury in mice after chest irradiation with ⁶⁰Co γ-rays at a single dose of 20 Gy. <b>Methods</b> C57BL/6 mice underwent chest irradiation with ⁶⁰Co γ-rays at a single dose of 20 Gy, followed by observation for the inflammatory reaction of the lung tissue in the early stage (within 15 d) and pulmonary fibrosis in the later stage (30 and 180 d). Enzyme-linked immunosorbent assay was used to measure the levels of C2, C3a, C4, and C5b-9 in the lung tissues at 1, 3, 7, 15, 30, and 180 d after irradiation. The expression of complement mRNA in BEAS-2B cells after irradiation was determined using RT-PCR. <b>Results</b> Radiation-induced lung injury in micepresented as inflammatory response in the early stage and fibrosis in the late stage. Complement C2, C4, and C5b-9 complexes were increased in the early period (3 or 7 d) after irradiation (<i>P</i> < 0.05), which might be associated with the inflammatory response induced by irradiation. During 3 to 180 d, complement C3a was significantly higher in the irradiated mice than in the control mice, suggesting a close relationship between C3a and radiation-induced lung injury. The irradiated cells showed increased mRNA expression of C2 and C3, with no changes in the mRNA levels of C4 and C5. <b>Conclusion</b> Different complement proteins have varying responses to radiation-induced lung injury, among which C3a is closely related to radiation-induced lung injury, suggesting that regulating C3a and its receptors may be a new way to prevent and treat radiation-induced lung injury.

Effects of 60Co γ-rays on brain injury and polarization of microglia/macrophages in mice / 中华微生物学和免疫学杂志

Objective:To analyze the damage in hippocampal tissues of mice after whole-body irradiation with high- or low-dose ionizing radiation and to investigate the roles of microglia/macrophages polarization in the injury.Methods:C57BL/6 mice were randomly divided into three groups: sham irradiation group, low-dose group (0.05 Gy) and high-dose group (7 Gy). Low- and high-dose groups were respectively treated by whole-body irradiation with single dose of 60Co γ-rays. Hippocampal tissues of the mice were collected at 6 h, 1 d, 3 d and 7 d after irradiation. The morphology, structure and apoptosis of neurons were detected by HE staining, Nissl staining and Tunnel staining, respectively. RT-PCR and immunofluorescence assay were performed to detect the expression of M1 and M2 microglial markers at mRNA and protein levels in hippocampus tissues. The cognitive and emotional behaviors of mice were evaluated one month after the irradiation by Morris water maze, open field test, elevated plus maze and tail suspension test. Results:There were morphological and structural changes in the nerve cells in the hippocampus region of mice after irradiation, accompanied by apoptosis. Acute injuries occurred at 6 h after radiation, alleviated at 1 d and 3 d, and persisted at 7 d in a dose-dependent manner. The results of immunofluorescence staining and confocal imaging analysis showed that compared with the sham irradiation group, the high-dose group showed increased number of microglia, down-regulated expression of M1 microglial markers and up-regulated expression of M2 microglial markers in the hippocampus at 6 h and 1 d after radiation, while M2 microglial markers decreased at 3 d and 7 d after irradiation. PCR results showed that the expression of M1 and M2 microglial markers at mRNA level in the irradiation groups increased at 6 h after irradiation, but there was no statistical significance. The expression of related proinflammatory/anti-inflammatory factors was significantly up-regulated. The results of behavioral experiments showed that compared with the sham irradiation group, there was no statistical difference in cognitive or emotional behaviors at one month after irradiation.Conclusions:60Co γ-rays could damage mouse hippocampal tissues and result in the overexpression and different polarization patterns of microglia/macrophages in mice.

HPLC fingerprints and curcumin content of Curcuma Longa before and after irradiation by 60Co-γ / 中草药

Objective: To evaluate the effect of irradiation of 60Co-γ on composition change of Curcuma Longa through HPLC fingerprint analysis and determination of curcumin content. Methods: An Agilent SB-C18 column (250 mm × 4.6 mm, 5 μm) was used as the column. Curcumin content was measured according to the method in 2015 edition of the “Pharmacopoeia of the People’s Republic of China”. Acetonitrile-0.1% phosphoric acid aqueous solution was used as the mobile phase of fingerprint analysis, and it was programmed in a gradient elution model and the detection wavelength was 240 nm. The effects of irradiation on content of curcumin and components change were analyzed by paired t-test and similarity evaluation. And then the comprehensive evaluation of constituent difference of C. Longa before and after irradiation was carried out by HCA and PCA analysis. Further more the stability of curcumin content and fingerprint similarity of three patches samples storage for 3 and 6 months was compared with that of 0 month. Results: There was no significant difference before and after irradiation for curcumin content (P > 0.05). The fingerprints of C. Longae were established and 16 peaks were identified as the common peaks. The similarity of each batch before and after irradiation was more than 0.998 and the same batch before and after irradiation have better clustering consistency. The changes of curcumin content for 3 and 6 months compared with 0 month was less than 5%, and the fingerprint similarity was all greater than 0.95. Conclusion: The established fingerprinting method is accurate and reliable. 60Co-γ irradiation has no significant effect on the curcumin content and chemical composition consistency of C. Longa, which did not affect its stability. It was a good way to evaluate the 60Co-γ irradiation effect on C. Longa by combining fingerprint analysis and the index components content determination. This method can provide reference for the utilization of irradiation on C. Longa.

The knockdown of miR-23b enhances radio-sensitivity of lung cancer A549 cell via targeting PTEN / 中国肿瘤生物治疗杂志

@# Objective: To investigate the effect of miR-23b/PTEN molecular axis on radio-sensitivity of lung cancerA549 cells and its mechanism. Methods: Lung cancer cell lines NCI-H1650, NCI-H175, Calu-1, LT-P-A-2, MSTO-211H, A549 and human normal lung epithelial cell line BEAS-2B were selected. The expression level of miR-23b in lung cancer cell lines was detected by qPCR. Dual-luciferase reporter gene assay was used to verify the relationship between miR-23b and PTEN. Plasmids miR-23b mimics, miR-23b inhibitor and pcDNA3.1-PTEN were transfected intoA549 cells by lipofection; PTEN expression levels in cells was detected by WB. CCK-8, Transwell andAnnexin V-FITC/PI staining flow cytometry were used to detect the effect of miR-23b/PTEN axis on proliferation, invasion and apoptosis ofA549 cells treated with 60Co γ-ray. Results: miR-23b was upregulated in lung cancer cell lines with the highest expression in A549 cells (P<0.05 or P<0.01). Knockdown of miR-23b reversed the inhibitory effect of 3 Gy 60Co γ-rays on proliferation and invasion of A549 cells, and induced apoptosis (P<0.05 or P<0.01). Dual-luciferase reporter gene assay results confirmed that miR23b could negatively regulate PTEN (P<0.05). Furthermore, knockdown of miR-23b up-regulated PTEN expression level, and furhter enhanced the inhibitory effect of 3 Gy 60Co γ-ray on the proliferation and invasion of A549 cells as well as induced apoptosis of A549 cells (P<0.05 or P<0.01). Conclusion: Knockdown of miR-23b can enhance the radio-sensitivity of A549 cells, the mechanism of which is that 60Co γ-ray down-regulates the inhibitory effect of miR-23b on PTEN, thereby inhibiting the proliferation, invasion and inducing apoptosis ofA549 cells.

60Co-γ Radiation-induced Population Variation and Cluster Analysis of M1 Generation of Andrographis paniculata / 中国药房

OBJECTIVE： To study the effects of 60Co-γ radiation on the botanical traits and property of Andrographis paniculata， and to screen suitable irradiation dose. METHODS： The seeds of A. paniculata were irradiated by 60Co-γ rays with different irradiation doses（0，10，20，50，100，200，300 Gy）. The botanical traits indexes of A. paniculata as seed germination rate， root length， seedling rate， seedling height， leaf area， fresh weight， dry weight， stomata number of lower epidermis of leaf， and its property indexes as the contents of andrographolide， dehydrated andrographolide and chlorophyll， activity of T-SOD enzyme and CuZn-SOD enzyme， were determined after radiation. The coefficient of variation （CV） was calculated. The linear regression analysis was performed for seedling rate， and medial lethal dose was calculated. The correlation analysis was performed between the parameters of botanical trait and quality property with irradiation dose. Cluster analysis was conducted for M1 generation of A. paniculata in different irradiation dose groups by connection method combined with squared euclidean distance. RESULTS： Different irradiation doses showed different effects on botanical traits and property of A. paniculata. According to the average value of CV， the index of botanical traits was ranked as leaf area ＞ fresh weight ＞ dry weight ＞ plant height ＞ root length ＞ stomata number ＞ seedling rate ＞ germination rate； among different irradiation dose groups， the coefficient of variation was ranked as 50 Gy＞200 Gy＞100 Gy＞20 Gy＞10 Gy＞300 Gy＞0 Gy. According to the average value of CV， the index of property was ranked as dehydrated andrographolide content＞andrographolide content＞chlorophyll content＞CuZn-SOD enzyme activity＞T-SOD enzyme activity； among different irradiation dose groups， the coefficient of variation was ranked as 100 Gy＞50 Gy＞200 Gy＞20 Gy＞10 Gy＞300 Gy＞0 Gy. The medial lethal dose was 195.10 Gy. According to the botanical traits， M1 generation of A. paniculata of 7 dose groups could be divided into 4 types. According to the property， M1 generation of A. paniculata of 7 dose groups could be divided into 3 types. CONCLUSIONS： The suitable irradiation dose interval for irradiating A. paniculata is 50-200 Gy.

Comparison of the Effects of Different Sterilization Methods on the Contents of Index Components in Baras / 中国药学杂志

OBJECTIVE: To compare the contents of the index components in Baras treated by different sterilization methods for choosing the best sterilization method.METHODS: The mixed medicinal powder of Baras were treated by autoclave sterilization, circulating steam sterilization, dry heat sterilization and 60Co radiation sterilization, respectively. The contents of iso-chlorogenic acid A, iso-chlorogenic acid C, quercetin and piperine were determined by HPLC. The influence on the contents of the index components and microbe limitation were analyzed.RESULTS: The total number of bacteria and the counts of mold, yeast count and Escherichia coli in the samples treated by the four sterilization methods were all in compliance with the requirements of Chinese Pharmacopoeia. The total content of four kinds of index components in the samples treated by 60Co radiation sterilization method at dose of 4 kGy was slightly higher than that of others, with a loss rate of 1.21%.CONCLUSION: The 60Co radiation sterilization method is suitable for Baras drying with simple process, short drying time, and the ability to effectively retain all index components.

Effect of 60Co-γ ray irradiation on five active ingredients in Infantile Qingfei Pills / 中草药

Objective To establish HPLC method for simultaneous determination of five active ingredients of cucurbitacin B, cucurbitacin E, praeruptorin A, praeruptorin B, and praeruptorin E in Infantile Qingfei Pills (IQP), and study the contents changes of the five effective components in IQP before and after 60Co-γ ray irradiation. Methods The Agilent-C18 column (250 mm × 4.6 mm, 5 μm) was adopted and the detection wavelength was 230 nm and 321 nm with the flow rate of 1.0 mL/min. The mobile phase consisted of A (methanol: acetonitrile) and B (0.5% glacial acetic acid solution) for gradient elution, and column temperature was 25 ℃. Irradiation does of 2, 4, 6, 8 kGy were selected to irradiate IQP respectively. The contents of five active components in IQP were compared before and after irradiation, and the significant condition was observed by t-test. Results The linear range of cucurbitacin B, cucurbitacin E, praeruptorin A, praeruptorin B, and praeruptorin E were 0.049—1.247, 0.079—1.973, 0.056—1.406, 0.028—0.705, and 0.028—0.693 μg, The average recovery were 101.2%, 99.7%, 99.9%, 98.9%, and 100.5%, and RSD were 0.6%, 0.5%, 1.2%, 1.1%, and 1.2%, respectively. After irradiation of 2, 4, 6, and 8 kGy, the effective components contents of cucurbitacin B, cucurbitacin E, praeruptorin A, praeruptorin B, and praeruptorin E were changed. After t-test in groups, the content change of cucurbitacin B was significantly after irradiation over 6 kGy (P < 0.05). Conclusion The established method has a high recovery rate, good repeatability, which is simple and practical and can be used for quality control of IQP. The changes of each component are not significant with the radiation no more than 6 kGy, which can provide a reference for the sterilization of IQP.

Monte Carlo dosimetric study of the GZP 60 Co brachytherapy source with stainless steel applicator / 中华放射肿瘤学杂志

Objective To evaluate the effect of stainless steel applicator on dose distribution in GZP 60 Co brachytherapy source and to obtain the dosimetric parameters of the 60 Co source with stainless steel applicator. Methods Geant4 was employed to obtain the mean adsorption dose of the 60 Co brachytherapy source in the range of 0-10 cm, and the dosimetric parameters were calculated according to the formula proposed by AAPM reports TG43 and TG43U1. The 60 Co source was located in the center of a sphere water phantom with a radius of 30 cm. Results For channel 1 and 2 of GZP 60 Co source, the results of Λ with stainless steel applicator were 1. 014 cGyh-1 U-1( with a difference of 0. 5％ compared with non-applicator) , the results of Λ with stainless steel applicator for channel 3 were 0. 998 cGyh-1 U-1 ( with a difference of 0. 1％ compared with non-applicator) . The radial dose function in the range of 0. 5-10. 0 cm in a longitudinal direction was calculated and the fitting formula for the function was obtained. The polynomial function for the radial dose function and the anisotropy function with a of 0°-175° and an r of 0. 5-10. 0 cm were obtained. Conclusion The dosimetric parameters of the 60 Co source with stainless steel applicator are obtained, which provide more accurate reference data for clinical application. In clinical practice, the effect of stainless steel applicator on dose distribution should be considered.

Effect of 60Co-γ Ray Irradiation on the Four Components in Tongmai Granules / 中国药师

Objective: To study the effect of 60Co-γ ray irradiation on the contents of danshensu,protocatechuic aldehyde,puerarin and salviamolic acid B in Tongmai granules by HPLC. Methods: An Agilent ZORBAX Eclipse XDB C18(2) column (250 mm × 4. 6 mm,5 μm) was adopted and the wavelength of UV detection was 280 nm at the flow rate of 1. 0 ml·min-1. The mobile phase consisted of 0. 1% phosphoric acid (B) and acetonitrile (A) with gradient elution, and the column temperature was 35℃. Tongmai granules were irradiated by 60Co-γ ray respectively at 0, 2, 5,10 and kGy,the contents of the active ingredients were compared before and after the irradiation. Results: The linear range of danshensu,protocatechuic aldehyde,puerarin and salviamolic acid B was 0. 098-4. 925 μg, 0. 028-1. 411 μg, 0. 378-18. 882 μg and 0. 218-10. 888 μg, respectively. The average recovery was 99. 8% , 97. 7% , 99. 9% and 99. 9% , respectively. When the radiation dose was not more than 2 kGy, the contents of the five components did not change significantly (P>0. 05). After 5 kGy radiation, the contents of protocatechuic aldehyde and salviamolic acid B were signifi-cantly different (P <0. 05). Conclusion: The dose of 60Co ray should be controlled not more than 2 kGy, and the sterilization method is safe and effective for Tongmai granules.

Effect of 60Co Irradiation on 3 Active Ingredients in Compound Hemostatic Capsules / 中国药师

Objective: To establish an HPLC method for the simultaneous determination of 3 active components in compound he-mostatic capsules and investigate the content changes before and after 60Co-γ ray irradiation. Methods: An Agilent-C18column (250 mm×4. 6 mm, 5 μm) was adopted and the flow rate was 1. 0 ml·min-1. The mobile phase consisted of acetonitrile-0. 5% phosphor-ic acid solution (triethylamine was used to adjust pH to 6) with gradient elution, the detection wavelength was 254 nm (0-35 min) and 281 nm (35-55 min), and the column temperature was 35℃. The compound hemostatic capsules were irradiated at 5, 8 and 10 kGy, respectively, and the contents of the 3 active components in compound hemostatic capsules were compared before and after the radia-tion,and the t-test was applied to investigate the significance. Results: Typhaneoside,isorhamnetin-3-O-neohesperidin and tetrahydro-palmatine respectively within the concentration range of 0. 018-0. 11 mg·ml-1,0. 020-0. 120 mg·ml-1and 0. 011-0. 066 mg·ml-1 showed a good linear relationship with the peak area. The average recovery was 97. 7% , 98. 7% and 99. 3% with the RSD of 0. 9% , 0. 9% and 0. 5% , respectively (n=6). After the irradiation, the contents of the three active components in compound hemostatic capsules all showed changes. Under the dose of 8 kGy, the content changes of typhaneoside and isorhamnetin-3-O- neohesperidin showed no statistical significance (P>0. 05),and when the dose increased to 10 kGy,the content of tetrahydropalmatine exhibited sig-nificant difference (P<0. 05). Conclusion: The established determination method for compound hemostatic capsule shows such ad-vantages as high recovery, good repeatability, simple operation and promising separation, which can be used as a quality control meth-od for compound hemostatic capsules. The sterilization effect of 60Co-γ ray irradiation on compound hemostatic capsules is significant without notable changes in the active components. When the irradiation dose is equal to or below 5 kGy,the change of each component is not obvious,which can provide reference for the radiation sterilization of compound hemostatic capsules.

Study on anti-radiation effect of Citrus aurantium L. polysaccharides-B / 中国药理学通报

Aim To investigate the protective effect of Citrus aurantium L. polysaccharides-B(CALB) on ra-diation induced by 60Co γ-ray in mice. Methods The BALB/c mice were randomly divided into blank control group, radiation model group, CALB administration group (high, medium and low dose), and positive control group(black fungus polysaccharide,HP). The mice were administered orally for 30 days. After the last administration for three hours,the survival rates on the 2nd day and the 14th day of the blank control group and the irradiated mice after the single radioac-tive irradiation (7 Gy) with 60Co γ-ray were meas-ured. In addition, DNA content and micronucleus of bone marrow cells, SOD, GSH-Px activities, MDA content in serum, liver and brain tissues in mice, TChE activity in brain tissues and spleen and thymus index of mice were detected after one-time whole body irradiation with 60Co γ-ray (3 Gy). Results Each dose group of CALB could significantly improve the survival rate of irradiated mice,increase the DNA con-tent of mouse bone marrow cells and reduce the number of micronuclei in bone marrow cells. In addition, CALB could also increase the thymus and spleen index and the levels of SOD and GSH-Px in serum,brain and liver tissues of mice,and reduce the content of MDA. Conclusion CALB has protective effect on radiation injury,which can be used for further development and utilization of Fructus aurantii.

Effects of Different Dose 60Co Irradiation on Content of Isoimperatorin in Fengshi Bitong Capsule / 医药导报

Objective To establish a method for isoimperatorin in Fengshi Bitong capsule and to study the effects of different doses of 60Co irradiation on the content of isoimperatorin. Methods The Agilent C18 column(4.6 mm×150 mm, 5μm) was used with the mobile phase consisted of acetonitrile -water (41:59) ;The flow rate was 1.0 mL?min-1 ,the detection wavelength was at 310 nm,and the column temperature was at 30 ℃ . Results Isoimperatorin showed a good linear relationship within a range of 3.66896-183.448 μg? mL-1( r = 1.0000).The average rate of recovery was 96.42％, RSD = 2.61％.The content of isoimperatorin in Fengshi Bitong capsule did not change significantly when irradiated by 2, 5 and 10 kGy of 60Co. Conclusion The established methods can be used for isoimperatorin determination in Fengshi Bitong capsule with high specificity,accuration and feasibility.The influence was not significant when the irradiation dose was less than 10 kGy.

Mutation and fermentation optimization of Bacillus amyloliquefaciens for acetoin production / 生物工程学报

As a platform chemical, acetoin has a great potential of application in medicine and food industries. In order to improve the efficiency of acetoin production, Bacillus amyloliquefaciens was treated by atmospheric and room temperature plasma and gamma rays. Two-round screening was adopted for obtaining positive mutants, and the best mutant B. amyloliquefaciens H-5 produced acetoin up to 68.2 g/L in shake flask. Then, culture conditions were optimized in 5-L fermentor to enhance acetoin production. Finally, 85.2 g/L acetoin was produced by B. amyloliquefaciens H-5, which was increased by 26.8% compared with that of the original strain B. amyloliquefaciens FMME088. These results indicated that the high-producing strain can be obtained efficiently by compound mutagenesis, which has a promising prospect for commercial scale process.

Effect of 60Co-γ Irradiation on the Stability of Isoniazid / 中国药师

Objective:To establish an HPLC method for the determination of isoniazid and explore the impact of 60Co-γ on the sta-bility of isoniazid. Methods:A Diamosil C18(2) column(250 mm×4.6 mm,5 μm) was used. The mobile phase consisted of metha-nol and 0.2% phosphoric acid(2 :98). The flow rate was 1.0 ml·min-1,and the detection wavelength was at 254 nm. The isonia-zid solutions at different accurate initial concentration(10,50,100 μg·ml-1,100 μg·ml-1+saturated N2,100 μg·ml-1+satu-rated O2,100 μg·ml-1+10% tert-butanol and isoniazid powder) were irradiated by 60Co-γ at different doses (0.3,0.6,1,2.5, 5,10 kGy). And then,the concentrations of remained isoniazid were determined by HPLC. Results: Isoniazid was in a good linear relationship within the range of 1-200 μg·ml-1(r=0.999 0) with the recoveriy of 98.13% (RSD=0.65%). The degradation rate of isoniazid was affected by the initial concentration and the radiation dose,which increased with the increase of absorption amount and increased with the concentration increase,and the powder almost had no degradation. The degradation of isoniazid+O2significantly de-creased,and that of isoniazid+N2and isoniazid+tert-butanol was inhibied to a certain extent. Conclusion:Isoniazid is sensitive to 60 Co-γ irradiation,and its degradation is higher with lower concentration and higher irradiation dose.

Effects of different radiation on chromosome aberration in human lymphoblastoid cells / 中国职业医学

OBJECTIVE: To compare the effects of ~(56)Fe~(17+),~(12)C~(6+)ion beams and~(60)Co γ rays on chromosome aberration in human lymphoblastoid cells. METHODS: The human lymphoblastoid cells were divided into 0. 1,0. 3,0. 5,0. 7,1. 0,2. 0 Gy irradiated groups and 0. 0 Gy control group. They were separately exposed to ~(56)Fe~(17+)ion beams( linear energy transfer was 400. 0 ke V/μm),~(12)C~(6+)ion beams( linear energy transfer was 26. 0 ke V/μm) and~(60)C γ rays. Chromosome specimens were harvested 48 hours after irradiation. The effects of different radiation on dicentric plus centric ring( “d + r”) aberration rate and chromosome aberration in human lymphoblastoid cells were detected by light microscope with artificial counting. RESULTS: The “d + r”aberration rates induced by 0. 3-2. 0 Gy ~(12)C~(6+)ion beams were significantly higher than those of ~(56)Fe~(17+)ion beams and~(60)Co γ rays at the same dose( P < 0. 017). Chromosome aberration cell rates of 0. 1-2. 0 Gy ~(12)C~(6+)ion beams were significantly higher than those of ~(56)Fe~(17+)ion beams and~(60)C γ rays at the same dose( P < 0. 017). At the dose range of 0. 0-2. 0 Gy,chromosome aberration effects of three kinds of radiations were gradually increased( P < 0. 01). The relative biological effectiveness of ~(56)Fe~(17+)ion beams was lower than that of ~(12)C~(6+)ion beams.CONCLUSION: The chromosome aberration induced by ~(12)C~(6+)ion beams was more serious than that of~(60)Co γ rays and ~(56)Fe~(17+)ion beams.

The biological effects of 125I seeds and 60Co γ-rays on A549 and BEAS-2B cells / 中华放射医学与防护杂志

Objective To investigate the biological effects of 125I seeds and 60Co γ-rays on the non-small cell lung cancer cells A549 and the normal bronchial epithelium cells BEAS-2B.Methods A549 and BEAS-2B cells were irradiated with 125I seeds and 60Co γ-rays.The survival fraction was detected by colony formation assay.The cell cycle and cell apoptotic ratio were detected by flow cytometry.The expression of cell apoptotic related proteins was examined by western blot.Results After irradiation with different doses,the survival of A549 cells irradiated with 125I seeds was lower than that irradiated with γ-rays (t =6.06,9.42,4.90,P ＜0.05).After irradiation with 4 Gy of 125I seeds and 60Co γ-rays,the G1 phase percentages of A549 cells were 70.67％ ± 1.49％ and 59.59％ ± 0.71％ (t =10.77,P ＜ 0.05),and the apoptotic ratios of A549 cells were 18.09％ ±0.73％ and 9.81％ ±0.16％ (t =19.40,P＜ 0.05),respectively.125I seeds irradiation remarkably up-regulated the expressions of Bax and cleaved Caspase-3 proteins,down-regulated the expression of Bcl-2 proteins compared with 60Co γ-rays irradiation on A549 cells.However,the apoptotic ratio and the expressions of apoptosis-related proteins in BEAS-2B cells had little difference between two types of radiation.Conclusions The anti-proliferative effect of 125I seeds irradiation on A549 cell is more remarkably than that of 60Co γ-rays.The imbalance of Bcl-2/Bax ratio and the eventually activation of Caspase-3 proteins may play an important role in the anti-proliferative effect induced by the continuous low dose radiation of 125I-seeds.

Effects of 60Co Irradiation with Different Doses on the Volatile Components in Huodan Pill / 中国药房

OBJECTIVE:To establish a method for the contents determination of volatile components in Huodan pill and study the effects of 60Co irradiation with different doses on the volatile components in Huodan pill. METHODS:GC-MS was used to de-termine the contents of patchouli alcohol and the ralative contents of β-patchouliene,α-guaiacene,seychellesene,α-patchouliene,α-bulnesene,5,11-diene guaiacyl,and unidentified objects in Huodan pill after 60Co irradiation with different doses. Column was HP-5MS by programmed temperature,volume temperature was 250℃,split ratio was 50:1,detector was triple quadrupole mass spectrometer detector,MS1 quadrupole temperature was 150℃,MS2 was 150℃,carrier gas was helium,flow rate of column was 1.2 ml/min,and the volume injection was 1 μl;ion source was EI,bombarding energy was 70 eV,temperature of ion source was 230℃,temperature of transmission line was 280℃ and scan range was 50-500 amu. RESULTS:The linear range of patchouli alcohol was 0.103 1-2.062 0μg(r=0.999 4);RSDs of precision,reproducibility and stability tests were lower than 1.0%;recovery was 98.05%-102.32%(RSD=1.8%,n=9). 60Co irradiation with different doses had little effects on the 8 volatile components in Huodan pill. CONCLUSIONS:60Co irradiation can be used for sterilization on Huodan pill,but irradiation dose should be controlled. The method is simple,good reproducibility,and can be used for the contents determination of volatile components in Huodan pill.

Dose-response of S100A4 gene expression in human lymphocytes at different time points after irradiation / 中华放射医学与防护杂志

Objective To investigate the dose response of S100A4 gene expression in the irradiated lymphoblastoid cells AHH-1 at different time points post irradiation.Methods AHH-1 cells was exposed to different doses(0,1,3,5,8,10,15 and 18 Gy)of 60Co γ-rays,and its mRNA levels of S100A4 was detected by reverse transcription PCR and real-time PCR at 4,8,12,24,48 and 72 h after irradiation.Results Within the range of applied doses,the level of S100A4 gene expression was upregulated with a good dose-response (R2 =0.79-0.93,P ＜ 0.05) and had obvious difference at different time points (F =8.91,P ＜ 0.01).Conclusion S100A4 gene expression at transcriptional level could be detected easily and had optimum dose-responses at certain time points after irradiation,and hence is applicable as a dosimeter.

Ion exchange-based treatment of 60Co contaminated well-water for storing γ irradiation source / 中华放射医学与防护杂志

Objective To select an efficient ion exchange resin to purify the 60Co contaminated well-water for storing radioactive source and to ensure the radioactivity of 60Co in treated well-water below 10 Bq/L.Methods The radioactivity of 60Co in the water samples was measured by using the potassium cobaltinitrite coprecipitation-β counting method.The treatment efficiencies of two different ion exchange resins for the simulated 60Co-bearing waste water were compared to select a better one to dispose of the 60Co contaminated well-water.Results The treatment efficiency of MBD-15-SC mixed ion exchange resin was about 5.8 times higher than ZGCNR50 strong-acid cation exchange resin.The radioactivity of 60Co in the contaminated well-water could be reduced from 4.16 × 105 Bq/L to 1.16 Bq/L by two-stage sorption of MBD-15-SC mixed ion exchange resin.Conclusions Using several times of two-stage MBD-15-SC mixed ion exchange resin could effectively purify the 60Co contaminated well-water.The quality of the treated well-water could meet the sewage discharge standards.