ABSTRACT
Background: Several genetic polymorphisms of adiponectin have been associated to metabolic diseases as obesity and co-morbidities. Aim: To investigate if there are associations between +45TG, +276GT, -11,377CG y -11,391GA adiponectin SNPs (single nucleotide polymorphism) with obesity in a Chilean children population. Material and Methods: A case-control study was performed in 241 obese and 126 normal weight children (7-11 years old) from the urban community of Hualpén, Biobío region. Children were classified as normal or obese, according to age and gender-specificpercentiles defined by Centerfor Disease Control and Prevention (CDC). The analysis of serum markers was carried out using commercial kits. Adiponectin polymorphisms were determined through a High Resolution Melting (HRM)-enabled real time PCR and by DNA fragment sequencing. Results: The observed allelic frequencies of the studied SNPs were over 11%. The 11,377CG polymorphism was associated with a high risk of obesity, calculated by the additive inheritance model (odds ratio = 1.389, 95% confidence interval: 1.001-1.929,p = 0.049). Conclusions: Obese school children of the Biobío Region, have an increased risk of carrying the susceptibility allele polymorphism 11377CG of adiponectin gene.
Subject(s)
Child , Female , Humans , Adiponectin/genetics , Nutritional Status/physiology , Obesity/genetics , Polymorphism, Single Nucleotide , Case-Control Studies , Chile , Gene Frequency , Genetic Predisposition to Disease , Genotype , Odds Ratio , Risk FactorsABSTRACT
Este relato de caso descreve o tratamento ortodôntico de um paciente jovem, portador de má oclusão de Classe II, 1ª divisão de Angle, com apinhamento moderado na região anterior superior e retrusão mandibular. A Classe II foi tratada com Aparelho de Protração Mandibular modificado, associado a aparelhos fixos pela Técnica Lingual.
Subject(s)
Humans , Male , Adolescent , Orthodontic Appliances/trends , Malocclusion, Angle Class II/therapy , Orthodontics, CorrectiveABSTRACT
O presente artigo relata o tratamento ortodôntico de duas pacientes adultas portadoras de más oclusões com ausência múltipla de elementos dentários. Esses casos, em sua maior parte, demandam o concurso de outras especialidades, onde a reabilitação protética desempenha um papel essencial.
Subject(s)
Humans , Female , Adult , Jaw, Edentulous, Partially , Dental Occlusion , Denture, Partial , MaxillaABSTRACT
Não é raro que indivíduos procurem o tratamento ortodôntico com más oclusões mutiladas pela perda de molares inferiores. Essa situação leva o ortodontista a pensar sobre a melhor opção de tratamento: fechar o espaço com recursos de ancoragem do seguimento anterior ou abrir espaços para reabilitação com prótese. O objetivo do presente artigo é ilustrar por meio de dois casos clínicos a mesialização unilateral de molares inferiores usando o Aparelho de Protração Mandibular IV (APM IV) como ancoragem. O APM IV assimétrico mostrou ser um recurso eficaz na resolução de tais casos, sendo uma solução segura e de baixo custo.
Subject(s)
Humans , Female , Adolescent , Orthodontic Appliances , Molar , Mesial Movement of Teeth , Orthodontic Anchorage Procedures/methodsABSTRACT
Objective To evaluate the effects of exogenous adenovirus-mediated gene transfer of human apM1 gene (Ad-apM1) on malonaldehyde (MAD) and superoxide dismutase (SOD) levels in human umbilical vein endothelial cells (HUVEC). Methods HUVEC proliferation was measured by MTT after infection by AdapM1. Adiponetin protein level in cell culture medium of HUVEC cells infected with Ad-apM1 was detected by double antibody sandwich ELISA. The levels of MAD and SOD were measured by chromatometry. Results MTT assay showed that Ad-apMl had no effect on HUVEC proliferation. Human adiponectin protein levels in cell culture medium significantly increased after HUVEC was infected with Ad-apM1 for 24, 48, and 72 h, along with decreased MAD and increased SOD ( all P<0.05 ). MAD levels markedly increased and SOD levels decreased after HUVEC were incubated with 100 μmol/L H2O2 for 6, 12, and 24 h, and these reactions were reversed by AdapM1 transfection (all P<0.05 ). Conclusions HUVEC infected with Ad-apM1 effectively secrete human adiponectin. Ad-apM1 exerts antioxidation effect and antagonizes H2O2 -induced endothelial injury.
ABSTRACT
A Ortodontia contemporânea dispõe de vários dispositivos e filosofias de tratamento para correção da má oclusão de Classe II. O presente trabalho tem como finalidade demonstrar a aplicação do aparelho de protração mandibular na correção da Classe II ortocirúrgica, onde ocorreu uma negativa por parte da paciente quanto ao tratamento cirúrgico. A eficácia do tratamento compensatório será ilustrada pelo relato de um caso clínico, no qual se obteve um excelente resultado dentário, esquelético e facial.
Subject(s)
Humans , Female , Adult , Orthodontic Appliances , Malocclusion, Angle Class III/therapyABSTRACT
Objective To evaluate the association of apM1 gene single nucleotide polymorphisms (SNP) with type 2 diabetes mellitus in Chinese population. Methods Odds ratios (OR) of apM1 gene SNP distribution were analyzed. The Meta-analysis software (RevMan 4.3.1) was employed for summarizing the studies,calculating the pooled OR and its 95% CI and testing the overall effects. Egger's test and fail-safe number for P=0.05 (Nfs<,0.05>) were performed for evaluating the publication bias. The sensitivity analysis by different effect models and sample sizes were employed for the reliability of Meta-analysis. Results Nine literatures were obtained, apM1 gene SNP45 showed remarkable heterogeneity among the studies (P<0.10). Sub-group analysis revealed that the discrepancy based on southern Chinese individuals was the main source of the total heterogeneity.The distribution frequencies of apM1 gene SNP45G, SNP45GG, SNP276G and SNP276GG were significantly higher in Chinese type 2 diabetes mellitus group than those in NGT group (P<0.05). Their pooled OR and 95% C/were 1.50[1.12,2.02], 2.15[1.53, 3.02],1.23[1.03, 1.46] and 1.26[1.00,1.59], respectively (all P<0.05). The distribution of apM1 gene SNP45TG and SNP276GT between type 2 diabetes mellitus and normal glucose tolerance group revealed no difference among these studies. The results of publication bias diagnostics and sensitivity analysis accounted for the reliability and stability of this Meta-analysis. Conclusion apM1 gene SNPs are strongly associated with type 2 diabetes mellitus in Chinese population. SNP45G and SNP276G seem to be risk factors for type 2 diabetes mellitus.
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Aim To investigate the expression of the lentiviral vector carrying a human apM1(hapM1) gene in 293 T cells and its bioactivity.Methods The CDS region of hapM1 gene was subcloned into the lentiviral vector from a cloning vector,and the transfer plasmid(PNL-hapM1-IRES2-EGFP)of the lentiviral vector was constructed.The three plasmids of the lentiviral vector: PNL-hapM1-IRES2-EGFP,HELPER,and VSVG were cotransfected to 293 T cells to produce the recombinant lentivirus;293 T cells were infected by the recombinant lentivirus and were identified with fluorescent microscope,RT-PCR and western blot.Inhibitory effect of the recombinant hapM1 on proliferation of vascular smooth muscle cells(VSMCs) was identified.Results The sequencing results showed that the sequence of the subcloned hapM1 gene was completely identical to that reported in GeneBank.The virus titre of the recombinant hapM1 lentivirus was 2.0?105 TU?L-1.After the recombinant lentivirus infected 293 T cells,RT-PCR and Western blot results showed that the expression of hapM1 in the hapM1-293 T group could be measured.The recombinant hapM1 inhibited the proliferation of VSMCs.Conclusions The recombinant lentivirus carrying a human apM1 gene was constructed successfully.The recombinant hapM1 could be expressed in 293 T cells and had its bioactivity.
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Objective Explore the value of APM histochemical stain technique in the diagnosis of amniotic fluid embolism(AFE).Method The pulmonary tissues taken from autopsies performed in 1988～2001 including 19 AFE cases and 3 babies died of amniotic fluid aspiratory pneumonia(positive control),10 women in labor died of other causes(negative control) were sectioned again and stained by APM method and HE method. Comparative statistical research was carried out on findings from the 2 staining methods. Results By APM stain,amniotic fluid elements could be found in all AFE cases and positive control cases as well as some negative control cases.In addition,we found 1 positive case neglected and 1 false negative case of AFE retrospectively.Statistical comparison revealed that APM stain can elevate the detection rates both for keratinized epithelium and mucus.Conclusion APM staining provided possibility of detecting some amniotic fluid elements that was not distinguished by HE stain. The APM stain is helpful and can be used for AFE diagnosis in routine histological examination.
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Objective: To clone human apM1 gene for the study molecular mechanism of the function of adiponectin.Methods: RT-PCR method was used to isolate,from the total RNA of human adipose tissue,cDNA fragment encoding adiponectin,which was linked into pGEM-T Vector,then amplified,and cloned.The sequence was analyzed to identify the recombinant plasmids.Results: The result of sequencing indicated that the sequence of the cloned cDNA was identical to that of the corresponding parts of the mRNA for human apM1 in GeneBank except 2 nucleotides.Conclusion: Human apM1 cDNA gene was successfully cloned.