ABSTRACT
Flavonoids are a large group of phenolic secondary metabolites havinga wide range of biochemical and pharmacological effects. Quantitative analysis of flavonoid profiles in the genus Actinidia, which has not been intensively conducted, is useful to a better understanding of the pattern and distribution of flavonoids. In the present work, a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed to profile the flavonoids, which was then used to determine the dynamic change of 17 biologically active flavonoids in the leaves of Actinidia valvata at the main growing stages, including glucuronides and acylated di- and triglycosides of flavonoids. The contents of flavonoid triglycosides were significantly higher than other flavonoids. The highest concentrations of kaemperol glycosides were observed in June, while other flavonoids showed highest concentrations in October. On the other hand, the contents of four isorhamnetin glycosides were increased sharply in September to October. The flavonoid profiles seem to be related to temperature, UV-B, and water deficit. Further studies are required to examine the functions of flavonoids in the Actinidia valvata and the underlying molecular mechanisms of actions.
Subject(s)
Actinidia , Chemistry , Chromatography, High Pressure Liquid , Methods , Flavonoids , Chemistry , Plant Leaves , Chemistry , Seasons , Tandem Mass Spectrometry , Methods , Ultraviolet RaysABSTRACT
Objective To establish a method for determining volatile components from Actinidia valvata Dunn .Methods A static headspace‐gas chromatography‐mass spectrometry (HS‐GC‐MS) method was used to analyze volatile components , and the separated peaks were identified by mass spectal library searching combined with retention index comparison .Results 42 volatile components were separated from Actinidia valvata Dunn and 25 of them were identified ,mainly including alcohols ,es‐ters ,aldehydes ,hydrocarbons and so on .Conclusion Combined with retention index calculation ,this method improved accuracy of qualitation of HS‐GC‐MS and provided scientific proof for the exploitation and utilization of Actinidia valvata Dunn .
ABSTRACT
Objective To establish a method for the determination of asiatic acid in the root of Actinidia valvata . Methods HPLC-VWD was used in the analysis .The column was Agilent HC-C18 (4 .6 mm × 250 mm ,5μm);the mobile phase was acetonitrile :(30 mmol/L) acetic acid amine solution (35:65);the flow rate was 1 .0 ml/min;the temperature of column was 25 ℃ ;the detection wavelength was set at 210 nm ;the injection volume was 25 μl;the running time was 35 min .Results <br> Asiatic acid was separated with interference in baseline .The linear range was 25 .30-506 .0 μg/ml with linear correlation of 0 .999 6 for asiatic acid .The result of intra-day and inter-day precisions were both within 5% (n=3) ,and the average recovery was 99.4% with RSD 1 .9% (n=6) .Conclusion The method was simple ,rapid ,accurate and convenient for quality control of asiatic acid in the root of Actinidia valvata .
ABSTRACT
Objective: To study the anti-tumor constituents from the roots of Actinidia valvata Dunn. Methods: Column chromatographic techniques were used to isolate and purify the chemical constituents of the plant and their structures were elucidated by spectroscopic analysis, including 1HNMR, 13CNMR and MS. The cytotoxic activities of some individual compounds were examined by MTT assay. Results: Ten triterpenoids: 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid(1), asiatic acid(2), corosolic acid(3), 2α, 3α, 23, 24-tetrahydroxyurs-12-en-28-oic acid(4), 2α, 3α, 13β, 24-tetrahydroxyurs-11-en-28-oic acid-13-lactone(5), 2α, 3α, 24-trihydroxyolean-12-en-28-oic acid(6), 2α, 3α, 19α, 24-tetrahydroxyurs-12-en-28-oic acid(7), 2α, 3α, 24-trihydroxyurs-12, 20(30)-dien-28-oic acid(8), 2α, 3β, 24-trihydroxyurs-12-en-28-oic acid(9), and oleanolic acid (10), and one plant sterol β-citosterol(11) were isolated from the CHCl3 fraction of the roots of Actinidia valvata Dunn. The cytotoxic activities of five compounds (1, 2, 3, 4, and 7) against A549, LOVO and HepG2 cell lines were evaluated. Conclusion: Compounds 3-8 are isolated from this plant for the first time. Compounds 2 and 3 possess cytotoxic activity against LOVO and HepG2 cell lines, and the cytotoxic activity decreases with the increase of polarity of individual compound.
ABSTRACT
Objective: To make a comparative study on the root and stalk of Actinidia valvata, so as to lay a ground for expanding medicinal part of Actinidia valvata. Methods: A comparative study was made between the root and stalk of Actinidia valvata on the following aspects: macroscopical properties, physical and chemical properties, contents of active constituents, and pharmacological actions. Results: The root and stalk of Actinidia valvata both showed positive reaction for alkaloids and saponin, and both had 280 nm absorption peak in UV-Vis spectrum. The aqueous extraction yield of the root and stalk were 5.35% and 5.68%, respectively; the contents of dihydrodehydrodiconiferyl alcohol were 0.005 01% and 0.006 41%, respectively. The IC50 of root and stalk extracts against HL60 were 62.39 mg and 75.51 mg, respectively; and against K562 were 70.47 mg and 77.46 mg, respectively. Their extracts had similar scavenging activity for free radicals and reductive effect; neither of them had analgesia actions. Conclusion: The root and stalk of Actinidia valvata have similar physical and chemical properties, contents of active constituents and pharmacological actions, suggesting that it is highly possible to substitute the root of Actinidia valvata with stalk for medicinal usage.