ABSTRACT
From March 1970 to December 1984, treatment results of 21 patients treated initially with curative surgery for adenoarcinoma of the colon and referred to the Department of Radiation Oncology, College of Medicine, Yonsei University, were analyzed retrospectively. Thirteen of 21 patients who were considered to be at high risk (i.e, stage B2 or above), received postoperative adjuvant radiation therapy. However, 2 of 13 patients did not complete their courses of radiotherapy as planned because of poor tolerance to radiotherapy or patient's refusal and were excluded from this study. Remaining 8 of 21 patients who did not receive postoperative radiotherapy, presented with recurrence at the time of referral and treated for palliation. In 11 patients who finished postoperativc radiotherapy, overall local failure rate was 9%(1/11) and the 5 year actuarial survival rate was 55%. Local failure rates by stage were 0(0/4), 14%(1/7) for stage B2+B3, C1+C2+C3 respectively and 0(0/2), 17%(1/6), 0(0/3) for stage C1, B2+C2, B3+C3 respectively.
Subject(s)
Humans , Adenocarcinoma , Colon , Disulfiram , Radiation Oncology , Radiotherapy , Recurrence , Referral and Consultation , Retrospective Studies , Survival RateABSTRACT
Using an automatic image analyser,we have determined 18 parameters(250 fields)about the glandular,cytologic and nuclear atypia of the colonic mucosa of well-differentiated tubular adenocarcinoma,of mild,moderate and severe atypical hyperplasia and of normal.Statistic re- sults showed that most parameters had remarkable differences among the 5 groups.By stepwise discriminatory analysis,we selected 4 parameters(NLM,NPM,NCR,GASD)which had ex- cellent coincidence rates(95.6% and 100%)with the actual pathological diagnosis in the 250 previous cases and 50 new cases.The computer diagnosis system provided an objective and simple method for the diagnosis of colonic adenocarcinoma and precancerous lesions.
ABSTRACT
Aim To investigate the growth-inhibition and the mechanism of apoptosis induced by sophoridine(SRI) on SW620 cells.Method MTT assay was used to detect the half-inhibition concentration(IC50),and fluorescence microscopy,electron microscopy,DNA fragmentation analysis,flow cytometry(FCM) were used to demonstrate the presence of apoptosis.Results SRI inhibited the growth of SW620 cells significantly in a dose-and time-dependent manner,and morphological characteristics of apoptosis were observed with condensation of nucleus,bubble of cytoplasm,fragment of nucleus.A DNA ladder pattern of internucleosomalfragmentation was observed.Compared with that of control group,the percentage of the G0/G1 phase and the Sphase cells increased after treated by SRI.SW620 cells were induced to undergo apoptosis and underwent G0/G1 arrest with exposure to SRI shown by FCM.Conclusions Sophoridine could induce the inhibition of cell growth by means of apoptosis in a dose-and time-dependent manner,and the blocking of G0/G1 phase of cells was involved in the mechanism of apoptosis.