ABSTRACT
This study was aimed to optimize the purification technology for Sambucus williamsii Hance. With the morroniside as a marker, the purification technology for Sambucus williamsii Hance was optimized by different types of macroporous resin. The results showed that the optimum purification technology was that, the extract of less than 1:250 (morroniside:resin) was adsorbed and the AB-8 resin was washed with distilled water, and then the morroni-side was eluted from the macroporous resin with 10% ethanol. And the content of the morroniside was more than 50%. It was concluded that the purification technology was simple, reliable, repeatable and suitable for industrial production.
ABSTRACT
OBJECTIVE:To draw a comparison among four extraction methods of total DNA from Corydalis saxicola in order to get high quality DNA sample.METHODS:The total DNA was extracted by palmityl trimethyl ammonium bromide method,enzyme-added detergent method,guanidinium isothiocyanate method,inertmacromolecular adsorptive column method,respectively.The purity(defined as RA260/A280)and the yield of the total DNA were determined.RESULTS:The RA260/A280 values of the DNA samples extracted by 4 methods were 1.832 9,1.699 2,1.812 2 and 1.813 6,respectively;and the extraction rates were 140.20 ?g?g-1,69.56 ?g?g-1,132.50 ?g?g-1 and 143.12 ?g?g-1,respectively.CONCLUSION:DNA samples extracted in 4 methods were all of high quality.The yield of DNA sample in enzyme-added extraction method was lower,while the guanidinium isothiocyanate method and the adsorption column method were rapid and simple and they are suitable for the test of samples of small size.