Airway Inflammation and Responses in the Bronchial Asthma Model in Sprague-Dawley Rats Sensitized by Ovalbumin / 결핵

BACKGROUND: To evaluate airway responses and inflammation to antigen in Sprague-Dawley rat asthma model, we examined airway responses, serial histologic changes of the lung, and the relationship between airway responses and airway inflammation after antigen airway challenge. METHODS: Sprague-Dawley rats were sensitized with subcutaneous injection of 10 microgram ovalbumin(OA). Antigen airway challenges were done 14 ~16 days after sensitization and the sensitized rats were sacrificed 1h(AE), 6 ~8h(AL) and 1day(AD) after airway challenge, to examine the histologic changes of the lung. Airway responses were measured by body plethysmograph and recorded by enhanced pause(Penh) as an index of airway obstruction 6 ~8h after antigen challenges. Nonsensitized controls(10 rats) were also challenged with antigen and sacrificed 1 day later. Histopathologic examination of two trachea, large bronchi, small bronchi, and vessels was performed to evaluate the severity of inflammation and eosinophilic infiltration with H&E stain. RESULTS: In 17 of 20 rats(85%) in both groups, we observed airway responses. Among them, an early response(ER) in 15 rats(75%), an dual response in 5(25%), and an late response(LR) only in 2 rats(10%) displayed. There were no significant differences in the severity of inflammation among the trachea, large bronchi, small bronchi and vessels in all groups after antigen challenge(p>0.05) and between early and late responders. The significant eosinophil infiltration was observed in 5 rats(50%) of AL(p0.05). CONCLUSION: Sprague-Dawley rats sensitized with subcutaneous injection of OA showed a significant airway responses to antigen challenge. But antigen challenges caused a little eosinophil infiltration and no significant airway inflammation. Asthma model of Sprague-Dawley rats could be useful for antigen-induced airway responses, but this model has a limitation for the study of human asthma because of no significant pathologic change.

Establishment of allergic airway response model in mice with intestinal microflora imbalance induced by dermatophagoides pteronyssinus allergens / 重庆医科大学学报

Objective:To establish the intestinal microflora imbalance mouse model to construct a novel allergic airway response model induced by dermatophagoides pteronyssinus allergens. Methods:Sixty BALB/c mice were divided into six groups. Mice in two groups were prepared for the analysis of the quantity of the intestinal bacterial flora and mice in other four groups for the analysis of allergic airway response. Results:Intestinal microflora of mice in antibiotic therapy group presented the weight loss and feces moisture increase. For the mice with intestinal microflora imbalance and induced by dermatophagoides pteronyssinus allergens,the number of total cells,lymphocytes,neutrophils and eosinophils in bronchoalveolar lavage fluid(BALF)significantly increased,the expression level of IL-4 increased,and the expression of GATA-3 mRNA in the lung tissue were upregulated. Conclusions:An allergic airway response model was established successfully by using dermatophagoides pteronyssinus allergens to induce mice with intestinal microflora disruption.