ABSTRACT
OBJECTIVE:To establish a method for the content determination of muscone in Aiweixin oral liquid. METHODS:After extracting sample by butanol,GC-MS was performed on the column of BD-17 MS with the volume temperature of 270 ℃;the carrier gas was helium with the sample size of 1 μl at the flow rate of 10 ml/min;the split ratio was 20∶1. EI was ion source with the electron energy of 70 eV,ion source temperature of 230 ℃ and quadrupole temperature of 150 ℃;the tuning mode was automatic tuning by quality full scanning with the threshold value of 30 in the range of 30-600 aum. RESULTS:The linear range of muscone was 12.216-61.08 ng(r=0.999 6);RSD of precision test was lower than 2% and the RSDs of reproducibility and stability tests were lower than 3%;the average recovery was 100.24%(RSD=1.58%,n=6). CONCLUSIONS:The method is sensitive, rapid and simple,and can be used for the content determination of muscone in Aiweixin oral lipid.
ABSTRACT
Objective To establish a quality standard for Aiweixin oral liquid. Methods TLC was applied to the identification of Caryophylli Flos and Crocin in Aiweixin oral liquid. Eugenol was analyzed by GC. Results TLC of Caryophylli Flos and Crocin had distinct separation of characteristic spots and there was no interference in negative comparison. The linear response ranges of Eugenol were between 0.052 17-2.086 8μg (r=0.999 9). The samples were steady within 24 h. The average recovery of eugenol was 95.7%, RSD=1.4% (n=6). Conclusion The method is simple, accurate and reliable, and can be used for the quality control of Aiweixin oral liquid.