ABSTRACT
In recent years, immunoassay has been widely used in traditional Chinese medicine. It plays an important role in the quality control, the safety evaluation, and the mechanism research of Chinese medicine or compound medicine. Among them, the premise and key of the application is the preparation of monoclonal antibodies of small moleculars. This review summarized preparation of complete antigen, animal immunization, establishment of hybridoma cell lines, and production of monoclonal antibody. Especially, the varieties of representative antigen and antibody reported in recent 20 years were combed, which will provide the reference for the research and application of small molecular monoclonal antibodies of Chinese materia medica.
ABSTRACT
Objective:To evaluate the immunogenicity of the recombinant plasmid pcDNA 3.1(+)/kgp_ cd.Methods:BALB/c mice were immunized with recombinant plasmid pcDNA 3.1(+)/kgp_ cd,KGP_ cd protein(control) or vector pcDNA 3.1(+)(control) by quadriceps injection or targeted submandibular gland(TSG) injection. Serum IgG and salivary sIgA levels were assayed by indirect ELISA after immunization. The expression of the protein KGP_ cd in quadriceps and submandibular gland was detected by immunohistochemistry techniques.Results: Serum specific anti-KGP_ cd IgG elicited by pcDNA 3.1(+)/kgp_ cd and KGP_ cd protein was significantly higher in both the quadriceps injection group and the TSG group than that in the pcDNA 3.1(+) group (P