ABSTRACT
Objective: To study the chemical constituents of Artemisia argyi. Methods: The chemical constituents were isolated by silica gel column chromatography and HPLC, and its structure were identified by their spectral data and physicochemical properties analysis. Results: Thirty-four compounds were isolated from A. argyi with the structures identified as 5-hydroxy-6,7,3’,4’- tetramethoxyflavone (1), eupatorin (2), p-hydroxy-acetophenone (3), raspberry ketone (4), zingiberone (5), 7-hydroxycoumarin (6), p-hydroxybenzoic acid (7), desacetoxymatricarin (8), 3α-hydroxy-1(10),4,11(13)-triene-12,6α-olide (9), jaceosidin (10), 7-hydrxyterpineol (11), cis-2,8-dihydroxy-p-mentha-1(7)-en (12), trans-2,8-dihydroxy-p-mentha-1(7)-en (13), artemisetin (14), scopoletin (15), arteminolide C (16), desacetylmatricarin (17), artecalin (18), 11,13-dehydrodesacetylmatricarin (19), 1,9-azelaic acid (20), 3-methoxy-tanapartholide (21), phaseic acid (22), seco-guaiaretic acid (23), 5,3’,4’-trihydroxy-6,7-dimethoxy-flavone (24), 1,7-pimelic acid (25), 10-epi-ajafinin (26), 3-epi-iso-seco-tanapartholide (27), austroyunnane C (28), artanomaloide (29), ligustolide A (30), seco-tanapartholide B (31), 3-dehydroxy-iso-seco-tanapartholide (32), 3α-hydroxyreynosin (33), dihydrophaseic acid (34). Conclusion: Compounds 4, 22, 25, 30, 33, 34 are separated from the Artemisia for the first time. Compounds 5, 7, 8, 11-13, 21, 23, 24, 26-28 are isolated from A. argyi for the first time.
ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of neochlorogenic acid,cryptochlorogenic acid,chlorogenic acid,caffeic acid,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in the leaves of Artemisia argyi Levl.et Vant..METHODS The analysis of 50％ methanol extract of A.argyi leaves was performed on a 30 ℃ Prevail C1s column (4.6 mm ×250 mm,5 μmn),with the mobile phase comprising of acetonitrile-water flowing at 1.0 rnL/min in a gradient elution manner,and the detection wavelength was set at 325 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥ 0.999 5),whose average recoveries were 98.28％-101.11％ with the RSDs of 1.04％-2.59％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of A.argyi leaves.
ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of neochlorogenic acid,cryptochlorogenic acid,chlorogenic acid,caffeic acid,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in the leaves of Artemisia argyi Levl.et Vant..METHODS The analysis of 50％ methanol extract of A.argyi leaves was performed on a 30 ℃ Prevail C1s column (4.6 mm ×250 mm,5 μmn),with the mobile phase comprising of acetonitrile-water flowing at 1.0 rnL/min in a gradient elution manner,and the detection wavelength was set at 325 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥ 0.999 5),whose average recoveries were 98.28％-101.11％ with the RSDs of 1.04％-2.59％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of A.argyi leaves.