ABSTRACT
ObjectiveTo study the effect-enhancing and toxicity-reducing activities of astragalus polysaccharide injection （APS） on U14 cervical cancer in model mice receiving X-ray treatment. MethodU14 mouse cervical cancer cells were cultured in vitro and injected into the right forelimb armpit of Kunming mice for constructing a subcutaneous tumor-bearing model of cervical cancer. The tumor-bearing mice were randomly divided into the model group， X-ray intervention（IR， 6 Gy） group， APS （10 mL·kg-1·d-1） group， and IR + APS group. Following the observation of the state， body mass， and food intake of mice in each group， the volume of the tumor was measured. The tumor cell cycle and apoptosis were determined by flow cytometry. The protein and mRNA expression levels of apoptosis-related proteins p53， B-cell lymphoma-2 （Bcl-2）， Bcl-2-associated X protein （Bax）， and cleaved cysteine-dependent aspartate-directed protease-3 （Caspase-3） in tumor tissues were assayed by Western blot and real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）. ResultThe comparison with the model group showed that mice in the IR group had poor mental status and reduced mobility. The IR group and IR + APS group exhibited reduced food intake and body mass since the 8th d （P<0.05， P<0.01） and narrowed tumor volume since the 9th d （P<0.01）. In the IR group， the proportion of cells in the G1 phase was increased， while the proportion of those in the S phase was decreased （P<0.01）. In the IR + APS group， the proportion of cells in the G1 phase rose， whereas the proportion of those in the G2 and S phases cells declined （P<0.05， P<0.01）. The apoptotic rates in both the IR group and IR + APS group were elevated significantly （P<0.01）. Compared with the model group， the IR group and IR + APS group displayed up-regulated cleaved Caspase-3 and Bax protein and mRNA expression in tumor tissues， but down-regulated Bcl-2 and p53 protein and mRNA expression （P<0.05， P<0.01）. Compared with the IR group， the mice in the IR + APS group had better mobility and hair， normal body mass， and increased food intake （P<0.05）. The tumor volume in the IR + APS group was reduced （P<0.05）. The proportion of cells in the G2 phase was reduced， but the proportion of those in the S phase was raised （P<0.05）. The apoptosis rate was increased （P<0.05）. The apoptosis-related protein Bax protein expression in the tumor tissue was up-regulated， while the protein expression levels of Bcl-2 and p53 were down-regulated （P<0.05， P<0.01）. ConclusionAPS maintains the life state of U14 cervical cancer model mice treated with X-ray and promotes tumor cell apoptosis， thus enhancing the efficiency and reducing toxicity.
ABSTRACT
Objective: To explore the synergetic effect and observe the safety of Astragalus Polysaccharide Injection combined with cytokine-induced killer cells (CIK cells) in the treatment of advanced NSCLC patients with qi deficiency syndrome. Methods: A total of 75 advanced NSCLC patients with qi deficiency syndrome in oncology department of First Teaching Hospital of Tianjin University of TCM enrolled in the study were randomized into two groups: the control group (CIK cells group) and the combined group (Astragalus Polysaccharide Injection + CIK cells group) by the random number table method. The control group: CIK cells were transfused in vein (100 mL each time, once every Monday, Wednesday, and Friday, five times in total, the total number of cells > 1× 1010/mL). The combined group: CIK cells treatment combined with astragalus polysaccharide injection intravenous drip (250 mg Qd, 10 d). Ten days is one cycle, both groups were received the second cycle treatment in a month later. Results: The combined group's disease control rate (DCR=CR + PR + SD) was 69.4%, higher than that of control group's (36.1%), and the difference was statistically significant (P < 0.05).The KPS of combined group's effective rate was 77.8%, higher than 55.6% in the control group, the difference was statistically significant (P < 0.05). After treatment, shortness of breath, spiritlessness, hypodynamia, spontaneous perspiration and speaking reluctantly had taken a turn for the better in combined group, especially in spiritlessness, hypodynamia, spontaneous perspiration, gospeaking reluctantlyt to improve obviously, and the difference was statistical significance (P < 0.05). The haematologic toxicity in II, III, IV degree and hepatic and renal toxicity in III, IV degree did not appear in two groups. Conclusion: The Astragalus Polysaccharide Injection combined with CIK cells could control tumor lesion's progress, and improve the patient's immune function, the symptom of qi deficiency syndrome, and body functional status due to itsbetter security.