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OBJECTIVE: To study the chemical constituents from the rhizome of Saururus chinensis. METHODS: The chemical constituents from the rhizome of Saururus chinensis were extracted by supercritical CO2 extraction and isolated by various chromatographic methods, such as silica gel, MCI and pre-HPLC. Their structures were elucidated by physico-chemical constants and spectroscopic methods. RESULTS: Seventeen compounds were isolated and identified as aurantiamide acetate (1), echinuline (2), (-) -(7R, 8R) -7-O-acetylpolysphorin (3), elemicin (4), isoelemicin (5), 1, 4-bis (3, 4-dimethyoxyphenyl) -2, 3-dimethyl-1, 4-butanedione (6), saucerneol D (7), (2R) -3-(3', 4 ', 5'-trimethoxyphenyl) -1, 2 -propanediol (8), grandisin (9), rel-(7R, 8R, 7 'R, 8'R) -3', 4'- methylenedioxy-3, 4, 5, 5 '-tetramethoxy-7, 7 -epoxylignan(10), zanthopyranone (11), (±) -eritro-1-(3, 4, 5 -trimethoxy) -1, 2 -propanodiol (12), threo-3, 4, 5 -trimethoxy-7-hydroxy-1'-allyl-3', 5 '-dimethoxy-8. O. 4'-neolignan (13), (+) -(8R) -(2, 6 -dimethoxy-4-propenylphenoxy) -1-(3, 4, 5 -trimethoxyphenyl) propan-1-one (14), meso-dihydroguaiaretic acid(15), (-) -galbacin (16), and (-) - (7R, 8R) -7-O-acetylraphidecursinol B (17). CONCLUSION: Compound 6 is a new natural compound. Compounds 1 - 2, 4 - 6, 8 - 9 and 11 - 13 are isolated from this plant for the first time.
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Objective: To study the chemical constituents of Zhuang medicine Sauropus rostratu. Methods: The compounds were isolated and purified by silica gel, polyamide, MCI, and Sephadex LH-20 chromatography. The structures of the compounds were identified on the basis of chemical and spectral methods. Results: Nineteen compounds were isolated from 75% ethanol extrct of S. rostratu and identified as n-triacontanol (1), 2,4-di-tert-butylphenol (2), β-sitosterol (3), daucosterol (4), 3,6-anhydro-2-deoxy- D-arabino-hexono-1,4-lactone (5), niacinamide (6), scopoletin (7), 3β-hydroxy-26α,27β-arbor-13(18)-ene-24-carboxylic acid (8), 2R*,3R*,5S*-trihydroxy-6R*-nonadecyltetrahydropyran-4-one (9), emodin (10), protocatechuic acid (11), 2,3-dideoxy-D-erythro- hex-2-enono-1,4-lactone (12), caffeic acid (13), 3-acetrlc-affeic acid (14), quercetin (15), D-galactose (16), mannitol (17), aurantiamide acetate (18), and kaempferol (19). Conclusion: Compound 8 is a new compound named sauropurostratic acid, and compounds 2,5-14,and 16-18 are isolated from this plant for the first time.
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OBJECTIVE: To study the chemical constituents of the leaves of Magnolia grandiflora Linn and their antitumor activities. METHODS: The constituents were isolated and purified by various chromatographic methods, including column chromatographies on silica gel, Sephadex LH-20, as well as recrystallization. Their structures were identified by analysis of physical and spectral data and confirmed by comparison of their spectral data with the reported values in the literature or those of authentic samples. The antiproliferative activities of the purified compounds were evaluated by MTT assay. RESULTS: Twelve compounds were isolated and identified as parthenolide(1), ergosterol peroxide(2), N-formyl-annonain(Z)(3Z), N-formyl-annonain(E)(3E), vitamin E quinone(4), β-sitosterol(5), 11,13-dehydrocompressanolide(6), scoparone(7), liriodenine(8), aurantiamide acetate(9), michelenollide(10), and 2,6,2',6'-tetramethoxy-4,4'-bis(2,3-epoxy-1-hydroxypropyl)biphenyl(11). CONCLUSION: Compounds 2, 3Z, 3E, and 4 are isolated from this plant for the first time. Compounds 9 and 11 are isolated from the plants in Magnoli Linn for the first time.
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Objective To investigate the chemical constituents of marine sponge A gelas mauritiana collected from the South China Sea .Methods The n-butanol extract of marine sponge A gelas mauritiana was separated and purified by repeated column chromatography on silica gel ,Sephadex LH-20 ,and reversed-phase high-performance liquid chromatography (RP-HPLC) .The chemical structures of those obtained compounds were determined on the basis of spectroscopic analysis and com-parison with reported data .The tumor cell growth inhibitory activity of these compounds towards human lung carcinoma cells A549 was tested .Results Eight compounds were isolated ,including agelasine A (1) ,agelasine B (2) ,epi-agelasine C (3) , (-)agelasine D (4) ,agelasine E (5) ,agelasine F (6) ,(-)ageloxime D (7) and aurantiamide acetate (8) .These compounds dis-played different level of tumor cell growth inhibitory activity towards cell A 549 in vitro .Compounds 1-3 showed significant ac-tivity towards cell A549 ,being similar to that of the positive control of adriamycin .Conclusion Compounds 1 ,2 ,4 ,5 ,6 ,8 were isolated for the first time from the sponge A gelas mauritiana .Cell A549 was selected for the first time for the activity evalua-tion of compounds 1~7 .Significant inhibition activity of compounds 2、3 may hold as a basis for further research .
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Objective: To study the chemical constituents from the root bark of Changium smyrnioides. Methods: Compounds were isolated by various kinds of column chromatographies on silica gel, Sephadex LH-20, and recycling preparative HPLC from the ethanol extract in the root bark of C. smyrnioides, and their structures were elucidated by the physicochemical characteristics and spectral analyses. Results: Fifteen chemical constituents were obtained and identified as imperatorin (1), phellopterin (2), xanthotoxol (3), 5-hydroxy-8-methoxy-psoralen (4), vanillic acid (5), alloimperatorin (6), psoralen (7), bergapten (8), 8-O-β-D-glucopyranosyl- 5-methoxylpsoralen (9), isopimpinellin (10), caffeic acid (11), aurantiamide acetate (12), vaginatin (13), β-sitosterol (14), and succinic acid (15). Conclusion: Compounds 6-13 are isolated from the plants in Changium Wolff for the first time.
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Objective: To isolate and identify the chemical constituents from the roots of Pulsatilla cernua. Methods: Compounds were isolated by various kinds of column chromatographies on silica gel, Sephadex LH-20, and recycling preparative HPLC, and their structures were elucidated by the physicochemical characters and spectral analysis. Results: Fifteen chemical constituents were obtained and identified as 5-hydroxy-2, 2-dimethyl-2H-chromene-6-carbaldehyde (1), pulsaquinone (2), aurantiamide acetate (3), stigmasterol (4), hexadecanoic acid (5), aurantiamide (6), pinoresinol (7), buplerol (8), neoechinulin A (9), (2S, 3S, 4R, 8E)-2-[(2'R)-2'-hydroxypalmitoylamino]-8-octadecene-1, 3, 4-triol (10), 5R-dihydro-5-hydroxymethyl-2(3H)-furanone (11), caffeic acid (12), methyl 6-dehydroxyl rosmarinate (13), hederagonic acid (14), and 5-hydroxy-6, 8- dimethoxycoumarin (15), respectively. Conclusion: Compound 1 is a new natural product, compounds 3, 6, 8-10, 13, and 15 are isolated from the plants in Pulsatilla Adans. for the first time, and compound 7 is isolated from this plant for the first time.
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OBJECTIVE: To study the chemical constituents of Emilia sonchifolia. METHODS: The chemical constituents were isolated by Sephadex LH-20 and silica gel column chromatographies. Their structures were elucidated by spectroscopic data and physical-chemical properties. RESULTS: Ten compounds were isolated and their structures were established as 5, 7, 3'-trihydroxy-4'-methoxy flavone-3-O-α-L rhamnoside(1), apigenin-6, 8-di-C-β-D-Glucopyranoside (2), quercetin-3-O-α-L-rhamnoside (3), quercetin (4), aurantiamide acetate (5), obtucarbamate A (6), friedelin (7), stigmasterol (8), β-sitosterol (9) and daucosterol (10). CONCLUSION: Compounds 1-2, 5-7, and 10 were isolated from this genus for the first time.