ABSTRACT
Objective@# To investigate the effects of platelet-rich fibrin (PRF) and acellular dermal matrix (ADM) on the repair of oral mucosal defects and to provide the basis for soft tissue growth in oral implant operations.@*Methods@#Thirty-six healthy male Japanese big ear rabbits were randomly divided into the PRF group, ADM group, Autograft group (autologous connective tissue transplantation group) and Control group (blank control group); each group contained nine rabbits. Between the midline and the hard palate maxillary incisors, in an 8-mm location preparation and a 10-mm standard mucosa defect, the ADM group, PRF and Autograft group were implanted with ADM, autologous PRF and autologous cornification mucosa, respectively, whereas the control group had wound gauze compression processing at 7, 14, and 21 days to determine the wound healing rate in the area selected by HE staining. The inflammatory grade and average epithelial thickness were observed, and the results were statistically analyzed.@*Results @#Compared with the control group, the PRF, ADM and Autograft groups had significantly advanced wound healing (P < 0.05). The wound healing degree in the PRF group was similar to that of the ADM group at all time points (P > 0.05). The wound healing degree in the PRF and ADM groups was lower than that of the Autograft group at each time point (P < 0.05). HE staining results showed that compared with the control group, the levels of inflammation in the PRF group, ADM group and Autograft group were reduced, and the difference was statistically significant (P < 0.05). Nevertheless, there was no significant difference between the PRF, ADM and Autograft groups (P > 0.05). The epithelial thickness in the ADM group was similar to that in the Autograft group (P > 0.05). The epithelial thickness in the ADM group was higher than that in the PRF group at 7 d and 14 d (P < 0.05), but there was no significant difference at 21 d (P > 0.05).@*Conclusion @#PRF and ADM have similar healing effects in repairing oral mucosa defects, and they can be used as soft tissue augmentation materials instead of connective tissue transplantation.
ABSTRACT
Objetivo: comparar injertos de tejido blando autólogos y alógenos para el tratamiento de defectos clase I de Seibert. Métodos: se seleccionaron 18 sitios quirúrgicos, de 14 pacientes, y se asignaron aleatoriamente. Fueron asignados ocho sitios quirúrgicos para recibir injerto autólogo de tejido conectivo, y nueve para recibir injerto alógeno de fascia muscular. Se tomaron cuatro modelos diagnósticos para las mediciones, uno al inicio, y luego al primero, al segundo y al cuarto mes después de la cirugía. Adicionalmente, se realizaron seis biopsias del grupo de fascia. Resultados: el grupo control presentó una profundidad inicial promedio de 2,29 ± 0,55 mm, y el experimental, 3,50 ± 0,85 mm. La ganancia en espesor al primero, al segundo y al cuarto mes fue de 71,2, 46,52 y 28,5% en la fascia, y de 56,53, 38,31 y 38,1% en el tejido conectivo, respectivamente. La fascia se reabsorbió 28,8, 53,48 y 71,5%, al primero, al segundo y al cuarto mes, y el tejido conectivo se reabsorbió 43,47, 61,69 y 61,69%, respectivamente. No hubo diferencias significativas entre los dos grupos (p>0,05). El análisis histológico mostró presencia de tejido colágeno denso, poco celular, entremezclado con el tejido conectivo del huésped. La fascia tuvo una mayor tendencia a la reabsorción que el tejido conectivo, y la ganancia al cuarto mes en ambos fue similar. Sin embargo, el tejido conectivo fue más estable en el tiempo. La fascia mostró menor morbilidad,e histológicamente se observó presencia de este injerto después de 20 semanas. Conclusiones: la fascia humana constituye una alternativa de tratamiento para corregir defectos de reborde en el sector anterior.
Aim: Compare tissue grafting from autologous and allogenous sources for the treatment of Seibert Class I border defects. Methods: 18 surgical sites from 14 patients were selected and randomly assigned. 8 surgical sites received connective tissue grafts from autologous donor sites, and 9 surgical sites received allogenous muscular fascia grafts. 4 diagnostic casts were taken for comparative study (baseline, 1 month, 2nd month and 4th month postsurgical). In addition, 6 tissue punch biopsies were taken from the fascia group to determine presence of fascia tissue. ResultsThe control group showed an average tissue depth of 2.29 ± 0.55 mm and the experimental group showed 3.5 ± 0.85 mm, at baseline. Tissue gain for the fascia group was 71.2%, 46.52% y 28.5% for the 1st, 2nd and 4th months respectively, and the control group presented 56.53%, 38.31% y 38.1% respectively. There were not statistically significant differences between the two treatments (p>0,05). Histological analysis of the fasciagroup showed dense collagen tissue and host connective tissue at the 4th month. Fascia tissue grafts seemed to reabsorb faster than the connective tissue from the control group and the gain in tissue size at the 4th month was similar in both groups. The fascia tissue group, however, seemed to present less morbidity and was found in the histological samples at the 20 week postsurgical measurements. The connective tissue grafts were more stable in the treatment of Seibert I border defects. Conclusions: Human muscular fascia tissue could be an alternative treatment for the correction of Seibert class I border defects.