ABSTRACT
@#AIM: To investigate the relationship of IL-6 gene -572C/G polymorphism in patients with type 2 diabetic retinopathy(T2DM)and diabetic retinopathy(DR)in Dali Bai population.<p>METHODS: The polymorphisms of IL-6 gene -572C/G was analyzed by polymerase chain reaction-restriction fragment polymorphisms assay(PCR-RFLP)in 150 patients with T2DM \〖NDR(without DR)group 57 cases, NPDR(with non-proliferative diabetic retinopathy)group 77 cases, PDR(with proliferative diabetic retinopathy)group 16 cases\〗 and 100 healthy control group in Dali Bai population. The genotypes frequency, allele frequency of IL-6-572C/G gene and clinical data were compared between groups. The statistics software SPASS22.0 was used for statistical analysis.<p>RESULTS: The genotype and allele frequencies of IL-6 gene -572C/G between groups were compared statistically significantly(<i>P</i><0.05); the incidence of T2DM in healthy control subjects with C allele significantly decreased in comparison with the carriers of G allele(<i>OR</i>=1.182, <i>95% CI</i>: 1.059-1.319, <i>P</i>=0.004); the incidence of DR in T2DM with G allele significantly increased in comparison with the carriers of C allele(<i>OR</i>=1.667, 95% <i>CI</i>: 1.195-2.326, <i>P</i>=0.003), but there was no statistical difference between PDR group with NPDR group for -572C/G polymorphism(<i>P</i>>0.05). There was statistical significant between T2DM group, NPDR + PDR and control groups in fasting blood glucose, triglycerides, body mass index(<i>P</i><0.05), PDR group was compared with NPDR group only in difference between fasting plasma glucose(<i>P</i><0.05). The hypertension in individuals suffering from T2DM increased in comparison with not combing hypertension(<i>OR</i>=3.730, 95% <i>CI</i>: 2.060-6.754, <i>P</i>=0.000), and suffering from DR increased obviously(<i>OR</i>=3.997, 95%<i>CI</i>:2.099-7.612, <i>P</i>=0.000). The clinical data showed no significant difference(<i>P</i>>0.05)among different genotypes.<p>CONCLUSION: These results suggest that the -572C/G polymorphism in the promoter of IL-6 gene is associated with T2DM and DR in Dali Bai population, but the clinical data is not coordinative risk factor. G allele is a risk factor in the pathogenesis of T2DM and DR, but not for the progress in NPDR to PDR process, C alleles is a protective factor T2DM and DR. Hypertension, fasting blood glucose, triglycerides, body mass index were risk factors for T2DM and DR, fasting blood glucose condition in DR progress has an important role.
ABSTRACT
@#AIM: To investigate the relationship of IL-6 gene -572C/G polymorphism in patients with type 2 diabetic retinopathy(T2DM)and diabetic retinopathy(DR)in Dali Bai population.<p>METHODS: The polymorphisms of IL-6 gene -572C/G was analyzed by polymerase chain reaction-restriction fragment polymorphisms assay(PCR-RFLP)in 150 patients with T2DM \〖NDR(without DR)group 57 cases, NPDR(with non-proliferative diabetic retinopathy)group 77 cases, PDR(with proliferative diabetic retinopathy)group 16 cases\〗 and 100 healthy control group in Dali Bai population. The genotypes frequency, allele frequency of IL-6-572C/G gene and clinical data were compared between groups. The statistics software SPASS22.0 was used for statistical analysis.<p>RESULTS: The genotype and allele frequencies of IL-6 gene -572C/G between groups were compared statistically significantly(<i>P</i><0.05); the incidence of T2DM in healthy control subjects with C allele significantly decreased in comparison with the carriers of G allele(<i>OR</i>=1.182, <i>95% CI</i>: 1.059-1.319, <i>P</i>=0.004); the incidence of DR in T2DM with G allele significantly increased in comparison with the carriers of C allele(<i>OR</i>=1.667, 95% <i>CI</i>: 1.195-2.326, <i>P</i>=0.003), but there was no statistical difference between PDR group with NPDR group for -572C/G polymorphism(<i>P</i>>0.05). There was statistical significant between T2DM group, NPDR + PDR and control groups in fasting blood glucose, triglycerides, body mass index(<i>P</i><0.05), PDR group was compared with NPDR group only in difference between fasting plasma glucose(<i>P</i><0.05). The hypertension in individuals suffering from T2DM increased in comparison with not combing hypertension(<i>OR</i>=3.730, 95% <i>CI</i>: 2.060-6.754, <i>P</i>=0.000), and suffering from DR increased obviously(<i>OR</i>=3.997, 95%<i>CI</i>:2.099-7.612, <i>P</i>=0.000). The clinical data showed no significant difference(<i>P</i>>0.05)among different genotypes.<p>CONCLUSION: These results suggest that the -572C/G polymorphism in the promoter of IL-6 gene is associated with T2DM and DR in Dali Bai population, but the clinical data is not coordinative risk factor. G allele is a risk factor in the pathogenesis of T2DM and DR, but not for the progress in NPDR to PDR process, C alleles is a protective factor T2DM and DR. Hypertension, fasting blood glucose, triglycerides, body mass index were risk factors for T2DM and DR, fasting blood glucose condition in DR progress has an important role.
ABSTRACT
Objective The study aimed to investigate 15 autosomal loci polymorphism(such as D3S1358 and TPOX)in Nujiang Bai nationality,in order to construct genetic basic data of Bai population and provide a foundation for population study and forensic cases. Methods Venous blood was collected from 124 unrelated Bai individuals in Nujiang,Yunnan province. DNA was extracted by classical organic solvent extraction. PCR technique was applied to amplify the sequence of autosomal loci. The products were measured by electrical fluoroscopy. The gene frequencies of 15 STR loci were investigated and genetic polymorphisms were analyzed. ResultsGene polymorphisms of 15 STR loci were detected and the distributions of genotype conformed with Hardy-Weinberg balance law(P > 0.05). The cumulative match probability(CPM)was 4.869×10-17,the cumulative excluding probability(CPE)was 99.999 99%,and the combined power of discrimination(CDP)was 99.999 97%. ConclusionThe 15 STR loci used in this study were highly polymorphic informative content in Bai population and could be applied to population study and forensic practice.
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Objective To explore the association between clusterin(CLU) gene rs11136000 poly-morphism and late-onset Alzheimer's disease ( LOAD) in Bai population from Dali Bai Autonomous Prefec-ture of Yunnan Province.Methods A case-control study including 109 LOAD patients and 120 normal con-trols matched for age,sex and level of education was taken in Dali Bai population.Polymerase chain reaction (PCR) and single nucleotide polymorphism (SNP) site testing were used to detect genotype and allele fre-quency of CLU SNP rs11136000.SPSS 17.0 was applied to analyze the data.Result ①The different fre-quency ( C:65.60%,T:34.40%,CC:38.53%,CT:54.13%,TT:7.34%) of CLU SNP rs11136000 genotypes and alleles distribution in Bai between LOAD patients and healthy controls showed no statistical significance (χ2=1.529, P=0.216;χ2=2.805, P=0.246) .②The serum total cholesterol ( TC) of LOAD patients was significantly higher than that in that of control group( t=2.508, P=0.013) .Conclusion The results suggest that CLU rs11136000 polymorphism may not be the susceptible gene of LOAD,and high serum total choles-terol is more common in LOAD patients.