Transport mechanism of bergenin aross MDCK-MDRl cell monolayer model / 中国药理学通报

Aim To study the transport mechanism of bergenin passing through blood-brain barrier ( BBB ) . Methods MTT assay was used to investigate the tox-icity of bergenin on MDCK-MDRl cells. Molecular docking was used to predict the binding mode and effect ability of bergenin with P-gp. In vitro MDCK-MDRl cell monolayer model was used to analyze trans¬port characteristics of bergenin and the effect of con¬centration, time and verapamil (a P-gp inhibitor) on the transport of bergenin. Results Bergenin was non-toxic to MDCK-MDRl cells within the concentration of 5 to 40 jjunol • L . There was hydrogen-bond and hy-drophobic interaction between P-gp and bergenin, and P-gp-bergenin was more stable than P-gp-verapamil. The P value of bergenin transported from AP to BL (PappAP

Analysis on Chemical Constituents in Rhizomes of Bergenia scopulosa by UHPLC-Q Exactive Focus MS/MS / 中国实验方剂学杂志

Objective:Ultra-high performance liquid chromatography coupled with quadrupole/electrostatic field orbital trap high resolution mass spectrometry （UHPLC-Q Exactive Focus MS/MS） was developed to rapidly analyze and identify the chemical components in the rhizomes of <italic>Bergenia scopulosa</italic>. Method:The 75% methanol extract of <italic>B</italic>.<italic> scopulosa</italic> rhizomes was analyzed on a Thermo Accucore aQ RP18 column （2.1 mm×150 mm， 2.6 μm） with the mobile phase of methanol （A） and 0.1% formic acid aqueous solution （B） for gradient elution （0-40 min 5%-95%A， 40-45 min 95%A）， the flow rate was 0.3 mL·min^-1 and the column temperature was at 30 ℃. The information of the chemical constituents was acquired in positive and negative ion modes by heated electrospray ion source （HESI）， and the scanning range was <italic>m</italic>/<italic>z</italic> 80-1 200. Result:A total of 66 chemical constituents were identified， including 2 free amino acids， 7 bergenin derivatives， 15 flavonoids， 15 organic acids， 25 glycosides， and 2 others. Conclusion:The chemical constituents in the rhizomes of <italic>B</italic>.<italic> scopulosa</italic> can be identified systematically， accurately and rapidly by this method. Among them， 8 compounds were unambiguously identified by comparing with reference substances （succinic acid， arbutin， gallic acid， protocatechuic acid， bengenin， catechin， chlorogenic acid and caffeic acid）， 51 compounds were found from <italic>B</italic>.<italic> scopulosa</italic> for the first time and 28 compounds were found from the genus <italic>Bergenia</italic> for the first time. This paper can provide an important basis for the further material basis clarification and quality assessment of <italic>B</italic>.<italic> scopulosa</italic>.

Discovery and preclinical development of IIIM-160, a Bergenia ciliata-based anti-inflammatory and anti-arthritic botanical drug candidate / 中西医结合学报

OBJECTIVE@#Bergenia ciliata (Haw.) Sternb. is used in the Indian traditional system of medicine to treat various ailments including rheumatism and to heal wounds. The objective of the present study was to perform a preclinical characterization of the B. ciliata-based botanical extract IIIM-160.@*METHODS@#IIIM-160 was chemically standardized and analyzed for heavy metal content, aflatoxins, pesticides and microbial load. The in vitro and in vivo efficacies were determined in suitable models of inflammation, arthritis and nociception. An acute oral toxicity study was performed in Swiss albino mice. A suitable oral formulation was developed and characterized.@*RESULTS@#Bergenin was found to be the major component (9.1% w/w) of IIIM-160. The botanical lead displayed inhibition of lipopolysaccharide-induced production of proinflammatory cytokines in THP-1 cells, with selectivity toward interleukin-6 (IL-6) and had an excellent safety-window. It showed anti-inflammatory, anti-arthritic and antinociceptive activity in animal models and was not toxic at oral doses up to 2 g/kg in Swiss-albino mice. The gastroretentive, sustained-release capsule formulation showed sustained-release of the bergenin over the period of 24 h, resulting in improved plasma-exposure of bergenin in Sprague-Dawley rats.@*CONCLUSION@#The dual-activity of IL-6 inhibition and antinociception marks the suitability of IIIM-160 for treating rheumatoid arthritis. This study will serve as the benchmark for further research on this botanical formulation.

Effect and mechanism of YAO medicine compound containing Cissus pteroclada on rheumatoid arthritis in rats and its Q-marker prediction / 中草药

Objective: To evaluate the anti-rheumatism effect, and predict the mechanism and Q-marker of YAO medicine compound containing Cissus pteroclada (Sifangteng in Chinese, SFT) in the treatment of rheumatoid arthritis (RA) in rats. Methods: SD rats were randomly divided into control group, model group, tripterygium glycosides group and SFT high/low dose (28.7, 7.2 g/kg) groups with eight rats in each group. Except the control group, the RA models in rats induced by Collange II collagen were established. The SFT group and the tripterygium glycosides group were given corresponding drugs by intragastric administration during the modeling period, while the other two groups were given the same volume of saline once daily for 28 d. The degree of foot swelling was measured and scored during the experiment. The levels of TNF-α and IL-1β in serum were measured by ELISA at the end of the experiment. SFT chemical components and predicting targets were searched and screened through TCMSP and Drugbank databases. The target of RA disease was searched by TTD database. The protein interaction network was constructed and visualized by String database and Cytoscape software, cluster analysis was analyzed by MCODE. GO and KEGG enrichment analysis was carried out using String database. Finally, combined with the validity and measurability of chemical components, the Q-marker of SFT was predicted. Results: Compared with the model group, the foot swelling of rats in SFT high and low dose groups and positive group was significantly reduced (P < 0.01), and the serum levels of IL-1β and TNF-α were significantly decreased (P < 0.01). There were 89 disease targets of RA. The pathogenesis of RA was related to abnormal cytokine-receptor pathway and RA pathway. A total of 31 components in SFT were screened and its 119 target proteins were predicted, 12 of them belong to disease targets were involved in 1 112 biological processes, such as regulation of stimulation response, regulation of cell proliferation, regulation of cell metabolism, regulation of intracellular signal transduction, and regulation of 113 signaling pathways, such as RA pathway and TNF pathway, which ultimately play a role in the treatment of RA. At the same time, 11 components were predicted to be Q-markers of SFT, including apigenin, resveratrol, bergenin, nitidine, osthol, linalool, ammidin, ethoxychelerythrine, coptisine, hesperidin, and sesamin. Conclusion: SFT can significantly reduce acute inflammation in RA rats. SFT may act on PTGS2-based targets through resveratrol and other components, and participate in regulation of RA pathway, TNF pathway and other inflammatory and immune pathways. Apigenin, resveratrol and bergenin, nitidine, osthol, linalool, ammidin, ethoxychelerythrine, coptisine, hesperidin, sesamin can be used as Q-markers for SFT quality control.

DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes

Objective: To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes. Methods: Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the compounds isolation. DPPH antiradical scavenging assay was performed in order to evaluate the antioxidant activity. Total phenolic content was evaluated using the Folin-Ciocalteu assay. The anthelmintic activity was screened on the bovine adult male forms of parasitic nematode Onchocerca ochengi, by the in vitro evaluation of the inhibition of adult worm motility and mortality. Worms were incubated in the presence of different concentrations of the plant extract and effects on survival were monitored after 24 and 48 h. Results: The preliminary phytochemical screening revealed the presence of phenolic compounds, saponins, steroids, tannins, and terpenoids. Bergenin and a mixture of phytosterol, β -sitosterol and stigmasterol were isolated from this extract and were identified by nuclear magnetic resonance, mass spectrometry and by comparison with published data. The crude methanol extract of C. populnea rhizomes showed a strong DPPH antiradical activity with a good amount of total phenolic content ((20.69±2.13) g gallic acid equivalent/100 g of extract) and significant anthelmintic activity comparable to the standard drug ivermectin. Bergenin was found to be inactive even after 72 h of incubation. Conclusions: This study constitutes the first report on the anthelmintic activity of this plant and supports the traditional use of C. populnea as a natural antioxidant and anthelmintic.

Anti-leukemia Activities and Mechanisms of Bergenin Derivative D-23 / 中国药学杂志

OBJECTIVE: To study the anti-leukemia activities and mechanisms of bergenin derivative D-23. METHODS: CCK-8 method was applied to investigate anti-tumor activities of D-23. Flow cytometry and immunofluorescence assay were used to observe the effects of D-23 on the apoptosis and autophagy in K562 and Jurkat human leukemia cell lines. Western blot analysis was used to investigate the mechanisms that compound D-23 induced tumor cell apoptosis and autophagy. RESULTS: Bergenin derivative D-23 could significantly inhibit the proliferation of K562 and Jurkat cells by inducing cell apoptosis and autophagy. The mitochondrial membrane potential was decreased,protein kinase B(Akt)and heat shock protein 70 (Hsp70) were inhibited,and the expressions of apoptotic related proteins caspase 3 and caspase 9 were activated. In addition, mammalian target of rapamycin (P-mTOR)(Ser 2448 and Ser 2481)protein was inhibited. CONCLUSION:Bergenin derivative D-23 shows obvious anti-leukemia activities by inducing cell apoptosis and autophagy. The apoptosis may be associated with the reduction of mitochondrial membrane potential and activation of caspase pathway. The autophagy may be related to the inhibition of Akt/mTOR signaling pathway.

DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes

Objective:To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes.Methods:Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the compounds isolation. DPPH antiradical scavenging assay was performed in order to evaluate the antioxidant activity. Total phenolic content was evaluated using the Folin–Ciocalteu assay. The anthelmintic activity was screened on the bovine adult male forms of parasitic nematode Onchocerca ochengi, by the in vitro evaluation of the inhibition of adult worm motility and mortality. Worms were incubated in the presence of different concentrations of the plant extract and effects on survival were monitored after 24 and 48 h.Results:The preliminary phytochemical screening revealed the presence of phenolic compounds, saponins, steroids, tannins, and terpenoids. Bergenin and a mixture of phytosterol, β -sitosterol and stigmasterol were isolated from this extract and were identified by nuclear magnetic resonance, mass spectrometry and by comparison with published data. The crude methanol extract of C. populnea rhizomes showed a strong DPPH antiradical activity with a good amount of total phenolic content ((20.69±2.13) g gallic acid equivalent/100 g of extract) and significant anthelmintic activity comparable to the standard drug ivermectin. Bergenin was found to be inactive even after 72 h of incubation.Conclusions:This study constitutes the first report on the anthelmintic activity of this plant and supports the traditional use of C. populnea as a natural antioxidant and anthelmintic.

Isolation and identification of chemical constituents from Disporum cantoniense / 中草药

Objective: To study the chemical constituents from Disporum cantoniense. Methods: Various column chromatographic techniques were used to separate and purify the chemical constituents and their structures were elucidated by spectral ananlysis. Results: Sixteen compounds were isolated and identified as 3-maleimide-5-oxime (1), 4-methylene-5-oxopyrrolidine-2- carboxylic acid (2), thymine (3), adenosine (4), 5'-deoxy-5'-methylamino-adenosine (5), ethyl-α-L-rhamnose (6), bergenin (7), 4-hydroxy- 2-methoxyphenyl-6-deoxy-α-L-talopyranoside (8), (-)-epicatechin (9), (6R,9R)-roseoside (10), 3-(4-hydroxy-3,5-dimethoxyphenyl) propane-1,2-diol (11), 3-hydroxy-5-(p-hydroxyphenyl) pentanoic acid (12), 1-ribityl-2,3-diketo-1,2,3,4-tetrahydro-6,7-dimethyl-quinoxaline (13), (6S,9R)-vomifoliol (14), 1-(3,4-dihydroxyphenyl)-2-hydroxye-thanone (15), and 1,2-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)- ethane (16). Conclusion: Compounds 1, 2, 5-9, and 12-16 are isolated from genus Disporum Salisb. for the first time, and compounds 1, 2, 5-10, and 12-16 are isolated from this plant for the first time.

Study on fingerprint of Saxifraga stolonifera herbs and determination of five components by HPLC / 中草药

Objective To establish a method to research the fingerprint and determine the ingredients of Saxifraga stolonifera by HPLC, which aimed at providing reference for the quality control of Saxifraga stolonifera. Methods Agilent Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm) column was used as the stationary phase, and the mobile phase consisted of acetonitrile (A)-0.1% phosphoric acid with gradient elution at the flow rate of 1.0 mL/min. The detection wavelength was 254 nm, and the column temperature was maintained at 35 ℃. The result would be analyzed by SOP of Similarity evaluation system for chromatographic fingerprint of TCM. Results The results obtained by the method of fingerprint analysis are good. In the fingerprints, 11 peaks were selected as the common peaks to evaluate the similarities among 21 batches of S. stolonifera collected from different regions, and five contents of them were indentified as protocatechuic acid, gallic acid, bergenin, quercetin-5-O-β-D-glucoside, and quercitrin. The similarities between standard herb and each determined herb showed a lot of differences from others. The determination method showed that there weregood linear relationships of five figured contents in the range of 0.052 8-0.844 8, 0.020 96-0.335 36, 0.241 6-3.865 6, 0.130 8-2.092 8, and 0.023 68-0.378 88 μg. Moreover, the recoveries rates of five figured contents are 96.64%, 100.72%, 96.62%, 103.71%, 96.75%,and all RSDs were lower than 2%. The contents of 5 components in the gathered herbs were determined by external standard method in the range of 0.07-0.40, 0.19-4.36, 1.42-5.98, 0.42-6.86, and 0.11-1.51 mg/g. Conclusion The method established in this study is simple, reliable and durable, which can provide a scientific basis for the quality control of S. stolonifera.

Comparative pharmacokinetics of bergenin, a main active constituent of Saxifraga stolonifera Curt., in normal and hepatic injury rats after oral administration / 中国天然药物

Bergenin, isolated from the herb of Saxifrage stolonifera Curt. (Hu-Er-Cao) has hepatoprotective, anti-inflammatory, antitussive, and neuroprotective activities. The aim of the present study was to establish a simple, rapid, and sensitive RP-HPLC method for determination of bergenin in rat plasma and compare its oral pharmacokinetic behaviors in normal and CCl-induced hepatic injury rats. With norisoboldine as an internal standard, chromatographic separation was performed on a C analytical column with acetonitrile and water (11 : 89, V/V) containing 0.1% formic acid as the mobile phase. A good linearity was obtained over the range of 100-10 000 ng·mL. The lower limit of quantification was 50 ng·mL. The developed method was successfully applied to a study of the pharmacokinetic difference of bergenin (100 mg·kg) between normal and hepatic injury rats after oral administration. Marked alterations of pharmacokinetic parameters in hepatic injury rats were observed. Compared to normal rats, the AUC of bergenin in hepatic injury rats was elevated to 2.11-fold and C was increased by 130%, whereas CL value was only 55% of the normal rats, suggesting that the systemic exposure of bergenin was significantly increased under hepatic injury status.

Chemical constituents from aereal parts of Symplocos chinensis / 中草药

Objective: To study the chemical constituents from the aereal parts of Symplocos chinensis. Methods: The chemical constituents were isolated and purified by various column chromatographies. Their chemical structures were identified by physicochemical properties and spectral analyses. Results: Fourteen compounds were isolated from the the aereal parts of S. chinensis and identified as daucosterol (1), quercetin (2), quercetin-3-O-α-L-rhamnopyranoside (3), isoquercetrin (4), rutin (5), kaempferol-3- O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (6), niga-ichigoside F1 (7), shimobashiraside C (8), bergenin (9), protocatechuic acid (10), chrysophanol (11), aralia cerebroside (12), 2α, 3β, 19α, 23- tetrahydroxy-12-en-28-ursolic cid (13), and 2α, 3β, 19α, 23-tetrahydroxy-12-en-28-olenolic acid (14). Conclusion: Compounds 3, 5, 6, 8-12 are obtained from the plants of genus Symplocos Jacq. for the first time, and compounds 2-6 and 8-12 were obtained from the aereal parts of Symplocos chinensis for the first time.

Synthesis and cytotoxic activity of 3, 4, 11-trihydroxyl modified derivatives of bergenin / 中国天然药物

To synthesize a series of 3-, 4-, and/or 11-trihydroxy modified bergenin derivatives and evaluated their cytotoxic activity in vitro. The phenolic hydroxyl groups of bergenin were protected by benzyl groups with benzyl bromide. Treatment of dibenzyl bergenin with the corresponding acid in the presence of EDC·HCl and DMAP in CH2Cl2, followed by hydrogenation over Pd/C catalysts, afforded derivatives of bergenin esters. All of the target compounds were identified by IR, MS, and (1)H NMR. Twenty-six novel and three known derivatives of bergenin esters were synthesized. Their cytotoxicity values were evaluated by the MTT assay on the inhibition of DU-145 and BGC-823 cells in vitro. Several triply-substituted (3a, 4a, 5a, 6a, 7a) and doubly-substituted (8b, 9b) bergenin derivatives exhibited higher cytotoxic activity than bergenin. The result showed that the size of substituents and the lipophilicity of the bergenin esters displayed an important role on their cytotoxic activity.

Chemical constituents of Penthorum chinense Pursh / 中国药学杂志

OBJECTIVE: To study the chemical constituents of Penthorum chinense Pursh. METHODS: Many chromatography means were used in separation and purification, and the structures of all compounds were identified by the means of spectroscopic analysis and physico-chemical properties. RESULTS Fourteen compounds were elucidated as: pinocembrin-7-O-β-D-glucopyranoside(1), kaempfer-ol(2), vanillic acid(3), quercetin-3-O-β-D-xylopyranosyl-(1→2) -β-D-galactopyranoside(4), kaempferol-3-O-α-L-rhamnopyranoside(5), pinocembrin(6), quercetin(7), afzelin(8), 11-O-galloylbergenin(9), bergenin(10), 4-galloylbergenin(11), protocatechuic acid(12), catechins (13), gallic acid(14). CONCLUSION: Compounds 1, 3, 6, 7, 8, 12, 13 and 14 are obtained from the title plant for the first time.

Optimization of Extraction Process of Kanglao Qingfei Granules by Orthogonal Test with Multi-indexes / 中国中医药信息杂志

Objective To optimize the extraction process of Kanglao Qingfei granules. Methods Orthogonal test design was used to study the influence of soaking time, water content, extraction time on extraction technology with bergenin's extraction rate, total polysaccharides's extraction rate and extract yield as evaluation indexes. Results The optimum extraction process conditions were as follows:the raw materials were immersed in water for 1.0 h and extracted two times under reflux, and the ratio of water volume to raw materials weight was 10 and 8 (mL/g), and extraction time was 2.5 h and 2.0 h, respectively. Conclusion The optimized extraction process was simple and stable, which can provide foundation for industrial production of Kanglao Qingfei granules.

Anti-hyperuricemic activity of bergenin and its mechanism / 安徽医科大学学报

Objective To explore the anti-hyperuricemia activity of bergenin in the model of hyperuricemic mice induced by potassium oxonate. Methods 60 Kunming male mice were divided randomly into six groups, which were normal control group;hyperuricemic model group;and hyperuricemic groups with 20 , 40 , 60 mg/kg berge-nin, and 5 mg/kg allopurinol. Mice were orally administered once daily with 250 mg/kg potassium oxonate for 7 continuous days to create the model, and then three doses of bergenin and allopurinol were orally initiated on the day 1 h after potassium oxonate was given, separately. Serum uric acid, creatinine and urea nitrogon levels, as well as urinary uric acid and creatinine levels were measured. mRNA and protein expression levels of mouse kidney u-rate transporter 1(URAT1), and glucose transporter 9(GLUT9) were also determined. Results Compared with hyperuricemic model group, bergenin significantly reduced serum uric acid, creatinine and urea nitrogon levels, in-creased 24 h uric acid and creatinine excretion, and fractional excretion of uric acid in hyperuricemic mice;mRNA and protein levels of mURAT1 and mGLUT9 were also markedly down-regulated. Conclusion Anti-hyperuricemia effect of bergenin is attributed to the enhancement of uric acid excretion and reversion of mouse urate transporters o-ver-expression.

Simultaneous determination of nine compounds in Bergenia purpurascens by HPLC-MS / 中国药学杂志

OBJECTIVE: To develop a method for the determination of arbutin, gallic acid, bergenin, protocatechuic acid, chlorogenic acid, catechin, epicatechin, epicatechin gallate, and ferulic acid in Bergenia purpurascens by HPLC-MS/MS. METHODS: The samples were separated on a Diamonsil C18 column (4.6 mm × 150 mm, 5 μm) by gradient elution using methanol and 0.2‰(V/V) formic acid aqueous solution as the mobile phase at a flow rate of 0.8 mL · min-1. The column temperature was room temperature. Multiple-reaction monitoring (MRM) scanning was employed for quantification with switching electrospray ion source polarity in negative mode. RESULTS: Complete separation was achieved within 10 min for the nine compounds (arbutin, gallic acid, bergenin, protocatechuic acid, chlorogenic acid, catechin, epicatechin, epicatechin gallate, ferulic acid): The nine regression equations showed linear relationships between peak areas and contents of each compound. The average recoveries (n=9) of the compounds listed above were 100.2%, 100.0%, 99.8%, 99.9%, 97.9%, 101.2%, 99.8%, 101.7%, 102.4% and RSD were 0.80%, 3.06%, 2.72%, 3.28%, 3.58%, 1.44%, 0.95%, 0.96%, 0.48%, respectively. CONCLUSION: The method is simple, accurate and has good reproducibility, thus can be used for the determination of the nine compounds in Bergenia purpurascens.

Transport characteristics and absorption promoting methods of bergenin across Caco-2 cell monolayer model / 中国药学杂志

OBJECTIVE: To study the transdermal absorption mechanism and Methods of absorption promotion of bergenin so as to optimize its safe, stable and effective osmotic pump preparation. METHODS: Caco-2 cell monolayer model was used to measure the apparent permeability coefficients(Papp) and transport rate of all samples considering the major relevant factors of osmotic pump preparation design of insoluble drugs. RESULTS: Pappvaried little while concentration of bergenin increased. The efflux rate(ER) was smaller than 1.5. Angelica dahurica extract, agnuside, Artemisia argyi oil, platycodin, oleanolic acid and ursolic acid had significant effects on the Papp and transport rate of bergenin. The inclusion compound and solid dispersion techniques could enhance the absorption of bergenin. The two common hydrophilic polymer excipients of osmotic pump preparation could not improve the membrane absorption rate and permeability of bergenin. CONCLUSION: The absorption of bergenin across Caco-2 cell monolayer model may be passive transport. The study reveals possible directions for design of bergenin osmotic pump preparation.

Identificação de bergenina e carotenóides no fruto de uchi (Endopleura uchi, Humiriaceae) / Identification of bergenin and carotenoids in the uchi (Endopleura uchi, Humiriaceae) fruit

O extrato obtido em metanol da polpa de frutos Endopleura uchi foi submetido a fracionamento utilizando-se técnicas cromatográficas convencionais levando ao isolamento de bergenina. Na análise da composição dos carotenóides foi evidenciada a predominância de beta-caroteno (16,57 mig.g-1). Os isômeros do beta-caroteno foram detectados, trans-beta-caroteno (89,3 por cento), 13-cis-beta-caroteno (8 por cento) e 9-cis-beta-caroteno (3 por cento). Considerando a importância do papel nutricional dos carotenóides e a bioatividade do glicosídeo bergenina, esse estudo sugeriu o potencial desse fruto como alimento funcional.

The extract obtained in methanol from the fruits pulp of Endopleura uchi was fractionated using conventional chromatographic tecniques to isolate bergenin. In the analysis of the composition of carotenoids was evidenced the predominance of beta-carotene (16.57 mug.g-1). The isomers of beta-carotene: trans-beta-carotene (89.3 percent), 13-cis-beta-carotene (8 percent) and 9-cis-beta-carotene (3 percent) were detected. Considering the importance of the nutritional role of carotenoids and the bioactivity of the bergenina, this study suggested the potential of this fruit as functional food.

Determination of Bergenin in Roots and Stems of Ardisia Crenata by HPLC / 中国中医药信息杂志

Objective To find out the differences of bergenin content in Ardisia crenata from different regions and its different parts,and suggest its medicinal parts. Methods The bergenin content in different parts of Ardisia crenata was identified by TLC and determined by HPLC. Results The difference of bergenin content in different parts of Ardisia crenata was significant,and that in stems was better than in roots,which in the leaves was the lest. Conclusion The stem was regarded as the available medicinal resources and is worth exploiting.

Determination of Bergenin in Dicha Kechuan oral liquid by HPLC / 中华中医药杂志

Objective:To establish a determination method of Bergenin content in Dicha Kechuan oral liquid by HPLC.Methods:The separation was performed on SHIMADZU VP-ODS C18 column(150mm?4.6mm,5?m),the mobile phase was carbinol-water(20:80) with a flow rate of 1.0ml/min.The column temperature was room temperature and the detection wavelength was 275nm.Results:The linear range of Bergenin was 0.2575-5.150?g,the regression equation of working curve was Y=812702.2X-80626.3,r=0.9998,the average recovery was 101.4% and RSD 1.67%(n=5).Conclusion:This method is simple,reproducible,and can be used for quality control of Dicha Kechuan Oral Liquid and the semifinished product.