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OBJECTIVE: To study the effect of selenium-enriched Bifidobacterium longum on alcoholic liver damage and its mechanism. METHODS: Alcoholic liver damage of female C57/BL6 mice were induced with 20% alcohol for 42 d, at the same time 50 or 100 mg•kg-1 selenium-enriched Bifidobacterium longum were administrated respectively. Mice livers were collected for calculating liver index, HE staining and measuring liver tissue malondialdehyde (MDA) and superoxide dismutase (SOD) level. Serum were collected for measurement of alanine transarninase (ALT), aspartate aminotransferase (AST),γ-glutamyltransferase (GGT) and tumor necrosis factor α (TNF-α) levels. The mRNA levels of liver SREBP-1c, AMPK and PPAR-α were measured with Real-time PCR. RESULTS: Selenium-enriched Bifidobacterium longum inhibited the increase in liver index and inflammation index induced with alcohol. Selenium-enriched Bifidobacterium longum also inhibited alcohol induced ALT, AST, GGT and TNF-α levels increase; decreased liver MDA levels and increased liver SOD levels. Selenium-enriched Bifidobacterium longum down-regulated SREBP-1c mRNA and up-regulated AMPK and PPAR-α mRNA levels of mice liver. CONCLUSION: Selenium-enriched Bifidobacterium longum protects mice from alcohol induced liver damage in a dose dependent manner. Its protective mechanism might be anti-oxidation, inflammation inhibition and improvement of lipid metabolism.
ABSTRACT
To investigate the protective effect of selenium-enriched Bifidobacterium longum preparation on rat IEC6 cells via the application of lipopolysaccharide (LPS)-induced IEC6 cell injury model. Methods The experiment was divided into five groups; negative control group treated with phosphoric acid equilibrium salt solution, model group treated with LPS, and low, medium, high concentration group in which IEC6 cells were treated with LPS plus 10, 30, 100 mg • L"1 water-soluble proteins from selenium-enriched Bifidobacterium longum respectively. After treatment with LPS for 24 hours, IEC6 cell viability, apoptosis, mitochondria membrane potential, and the expression of zonula occludens 1 (ZO-1) and Occludin were detected. Results LPS induced rat intestinal epithelial cell damage, such as the decrease of cell viability, the increase of cell apoptosis, the collapse of mitochondria membrane potential, and the decrease of cell tight junction protein expression. Water-soluble proteins from selenium-enriched Bifidobacterium longum inhibited LPS-induced IEC6 cell damage, decreased cell apoptosis and increased cell tight junction between cells. Conclusion Water-soluble proteins from selenium-enriched Bifidobacterium longum have protective effect on LPS-induced rat intestinal epithelial cell injury.
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Abstract Probiotics have provided benefits to general health, but they are still insufficient to dental health. Objective: This study aimed to evaluate milk supplemented with probiotic bacteria and standard milk, measured by levels of Streptococcus mutans (S. mutans) and Lactobacillus spp., in 3-4-year-old children after 9 months of intervention. Material and Methods: The study was a triple-blind, placebo-controlled, randomized trial. The sample was composed of 363 preschoolers attending five child development centers in Cali, Colombia. They were randomized to two groups: children in the intervention group drank 200 mL of milk with Lactobacillus rhamnosus 5x106 and Bifidobacteruim longum 3x106, and children in the control group drank 200 mL of standard milk. Interventions occurred on weekdays and information was gathered through scheduled clinical examination. The primary result was the number of colony forming units (CFU) of S. mutans and Lactobacillus spp. in the saliva. Secondary results were dental caries, rated by the International Caries Detection and Assessment System (ICDAS), dental plaque, pH, and salivary buffer capacity. Results: The proportion of S. mutans was lower in the intervention group compared with the control group after 9 months; however, the differences did not reach statistical significance (p=0.173); on the other hand, statistically significant differences between groups were found in the CFU/mL of Lactobacillus spp. (p=0.002). There was not statistically significant difference in the prevalence of dental caries for both groups (p=0.767). Differences between groups were found in the salivary buffering capacity (p=0.000); neither salivary pH nor dental plaque were significantly different. Conclusions: Regular consumption of milk containing probiotics bacteria reduced CFU/mL of Lactobacillus spp. and increased salivary buffering capacity at 9 months of consumption.
Subject(s)
Humans , Animals , Male , Female , Child, Preschool , Saliva/microbiology , Streptococcus mutans/isolation & purification , Probiotics/administration & dosage , Milk/chemistry , Lactobacillus/isolation & purification , Time Factors , Colony Count, Microbial , Reproducibility of Results , Treatment Outcome , Statistics, Nonparametric , Dental Caries/microbiology , Dental Caries/prevention & control , Dental Plaque/microbiology , Dental Plaque/prevention & control , Milk/microbiologyABSTRACT
Resumen: ANTECEDENTES: el estreñimiento, la distensión y el dolor abdominales son muy prevalentes en la población general y representan un reto terapéutico. Los probióticos han mostrado ser efectivos en el control de estos síntomas. OBJETIVO: investigar la utilidad clínica de la administración del simbiótico Bifidobacterium longum AW11 (5x109 UFC) y fructo-oligosacáridos de cadena corta (Fos cc. Actilight 2.5 g) en el alivio del estreñimiento y otros síntomas digestivos. PACIENTES Y MÉTODO: estudio abierto observacional en el que se incluyeron pacientes con síntomas digestivos de más de seis meses de evolución, sin enfermedad orgánica que explicara sus síntomas y que recibieron tratamiento con el simbiótico, un sobre diario durante ocho semanas. La intensidad y frecuencia de los síntomas se evaluaron mediante un cuestionario y las características de las evacuaciones mediante la escala de Bristol al inicio, a las cuatro y a las ocho semanas de tratamiento. RESULTADOS: se incluyeron 361 pacientes con síntomas digestivos. El promedio de la duración de los síntomas fue de 2.3±4.9 años, 96% tenía estreñimiento, 79% cumplió con criterios de síndrome de intestino irritable y 16% de estreñimiento funcional (Roma III). El número de evacuaciones semanales se incrementó de 3.8±3.3 iniciales a 5.9±3.0 en la semana 8 (p<0.00001). También mejoraron significativamente: el número de evacuaciones espontáneas completas, la consistencia de las heces (escala de Bristol) y la sensación global de bienestar y disminuyeron significativamente el esfuerzo al defecar, la distensión abdominal subjetiva, la frecuencia de dolor abdominal y la sensación de malestar abdominal. La proporción de pacientes totalmente satisfechos se incrementó de 6% al inicio a 78% en la semana 8. CONCLUSIONES: la administración diaria del simbiótico con B. longum y Fos cc. es eficaz para aliviar el estreñimiento y el dolor y la distensión abdominales.
Abstract: BACKGROUND: Constipation, abdominal pain and bloating are highly prevalent in general population and they constitute a therapeutic challenge. Probiotics are effective in the management of these symptoms. OBJECTIVE: To investigate in daily clinical practice if the administration of a supplement food containing Bifidobacterium longum AW11 (5x109 FCU) and short chain fructo-oligosaccharides (Fos cc. Actilight 2.5g) can relieve constipation and functional bowel symptoms. PATIENTS AND METHOD: An open and observational study was done including patients with digestive symptoms of more than six months of evolution, without organic disease explaining their symptoms and who received treatment with the synbiotic, daily during eight weeks. Intensity and frequency of symptoms were assessed by a questionnaire and the characteristics of evacuations by Bristol's scale at baseline and after four and eight weeks of treatment. RESULTS: There were included 361 patients with digestive symptoms lasting 2.3±4.9 years; 96% had constipation, 79% fulfilled Rome III criteria for irritable bowel syndrome an 16% for functional constipation. Treatment with the synbiotic increased frequency of bowel movements per week from 3.8±3.3 to 5.9±3.0 at week 8 (p<0.00001). There was a significant improvement also for: complete spontaneous bowel movements, stool consistency (Bristol scale), straining, bloating, frequency of abdominal pain, malaise, and global wellness. Percentage of patients with complete satisfaction increased from 6% in the initial visit to 78% at the week 8. CONCLUSIONS: Daily ingestion of a synbiotic (B. longum and Fos cc.) is effective to relieve constipation, abdominal pain and bloating.
ABSTRACT
This study was conducted to isolate lactic acid bacteria (LAB) from dog intestine and identify potential probiotic strains for canine use. One hundred and one LAB were isolated from feces of 20 healthy dogs. Acid, bile, and heat resistance along with adherence to Caco-2 cells and antimicrobial activity against pathogens were examined. To analyze immunomodulative effects, the production of nitric oxide (NO), TNF-alpha, and IL-1beta was measured using RAW 264.7 macrophages. Additionally, RAW BLUE cells were used to evaluate nuclear factor-kappaB (NF-kappaB) generation. Ultimately, three strains were selected as canine probiotics and identified as Lactobacillus reuteri L10, Enterococcus faecium S33, and Bifidobacterium longum B3 by 16S rRNA sequence analysis. The L10 and S33 strains showed tolerance to pH 2.5 for 2 h, 1.0% Oxgall for 2 h, and 60degrees C for 5 min. These strains also had strong antimicrobial activity against Escherichia coli KCTC 1682, Salmonella Enteritidis KCCM 12021, Staphylococcus aureus KCTC 1621, and Listeria monocytogenes KCTC 3569. All three strains exerted better immunomodulatory effects than Lactobacillus rhamnosus GG (LGG), a well-known commercial immunomodulatory strain, based on NO, NF-kappaB, IL-1beta, and TNF-alpha production. These results suggested that the three selected strains could serve as canine probiotics.
Subject(s)
Animals , Dogs , Humans , Bacteria , Bifidobacterium , Bile , Caco-2 Cells , Enterococcus faecium , Escherichia coli , Feces , Hot Temperature , Hydrogen-Ion Concentration , Immunomodulation , Intestines , Lactic Acid , Limosilactobacillus reuteri , Lacticaseibacillus rhamnosus , Listeria monocytogenes , Macrophages , NF-kappa B , Nitric Oxide , Probiotics , Salmonella enteritidis , Sequence Analysis , Staphylococcus aureus , Sulfalene , Tumor Necrosis Factor-alphaABSTRACT
Elaborou-se leite de búfala fermentado por Lactobacillus casei utilizando leite desnatado, adicionado de 12,00 de açúcar e fermentado por 24 horas. Após fermentação o produto foi suplementado com Bifidobacterium ongum e estocado durante 30 dias a 5 e 10ºC, sendo então avaliados os parâmetros de acidez, pH e viabilidade de L.casei e de B. longum. Realizou-se a análise de composição nutricional, cálculo do valor calórico e análise sensorial do produto saborizado. O leite fermentado suplementado com B. longum, apresentou acidez inicial de 0,69 e pH de 4,86. A viabilidade de L. casei inicial foi 11,00 log UFC/mL e de B. longum 10,46 log UFC/mL. Após 30 dias de estocagem refrigerada, a acidez e o pH estavam adequados e a viabilidade de L. casei e de B. longum ficou acima de 9,00 log UFC/mL. A análise da composição nutricional resultou nos valores (): proteína 3,63, carboidrato 21,86, gordura 0,10, resíduo mineral fixo 0,56, umidade 73,85 e matéria seca 26,15, com valor calórico de 103 Kcal/100g. Não houve diferença sifnificativa na preferência e na aceitação das amostras de diferentes sabores, sendo que o produto apresentou boa aceitação pela escala hedônica
It was elaborated fermented buffalo milk for L. casei utilizing skimmed milk added with 12,00% sugar andfermented by 24 hours. After fermentation, the product was supply with B. longum and storage during30 days at 5 and 10° C, being evaluated the acidity, pH and viability of L. casei and B. longum parameters.Were does the nutritional composition, calculate the caloric value and sensorial analysis of the flavoredproduct. The fermented milk presented initial acidity 0.69% and pH 4.86. The L. casei initial viability was11,00 log CFU/mL and B. longum 10,46 log CFU/mL. After storage of 30 days, the acidity and pH wereadequate and the L. casei and B. longum viability was above 9.00 log CFU/mL. The composition analysisresulted (%) protein 3.63, carbohydrate 21.86, fat 0.10, ashes 0.56, wet 73.85 and dry substance 26.15,with caloric value 103 Kcal/100 g. There wasn't significant difference to preference and acceptancebetween flavored samples, and have good acceptation by hedonic scale
Subject(s)
Bifidobacterium , Buffaloes , Lacticaseibacillus casei , Dairy Products/analysis , Milk , Probiotics , Cultured Milk ProductsABSTRACT
Bifidobacterium longum, indigenous bacteria of the gastrointestinal tract, is a species of anaerobic non-spore-forming gram-positive bacilli, and appears to play an important role in preventing overgrowth of pathogenic bacteria. This organism is very rarely isolated from clinical specimens. It is known to be very difficult to identify Bifidobacterium longum due to not only the variability in aerotolerance, colony morphology and stainability on gram staining, resulting the failure of distinguishing from other anaerobic non-spore-forming gram-positive bacilli by the conventional biochemical tests. Definitive identification of genus Bifidobacterium requires the analysis of the end-products of metabolism, volatile and non-volatile fatty acids, by gas-liquid chromatography (GLC). We report a case of sepsis caused by Bifidobacterium longum in a 19-year-old male who developed high fever, jaundice and hepatomegaly after he had been introduced with many small gold needles. Anaerobic non-spore-forming gram-positive bacilli were isolated from his blood and identified as Bifidobacterium longum by performing biochemical tests and the analysis of the metabolic end-products by GLC. He has been completely recovered after ticarcillin and metronidazole therapy. To our knowledge, this is the first report of incidental sepsis by Bifidobacterium longum in the world.