ABSTRACT
The chemical composition of the essential oil obtained by hydrodistillation from fresh leaves of Blepharocalyx salicifolius (Kunth) O. Berg (Myrtaceae) was analyzed by gas chromatography/mass spectrometry (GC/MS). Twenty-two compounds were identified and a predominance of sesquiterpenes was observed (twenty sesquiterpenes - 96.53 percent). This study presents for the first time the qualitative chemical composition of the essential oil from the leaves of B. salicifolius in their native habitat of Brazilian Cerrado. The qualitative chemical profile of essential oil obtained from the specimen in Cerrado differs from those obtained from specimens collected in biomes of southern parts of South America.
La composición química del aceite esencial obtenido por destilación al vapor de las hojas frescas de Blepharocalyx salicifolius (Kunth) O. Berg (Myrtaceae) se analizó por CG/EM (Cromatografia de Gases/ Espectrometría de Masas). Veintidós compuestos fueron identificados, predominantemente sesquiterpenos (veinte sesquiterpenos - 96.53 por ciento). Este estudio demuestra por primera vez, el análisis químico cualitativo de aceite esencial de hojas de B. salicifolius en su hábitat nativo del Cerrado brasileño. El perfil químico del aceite esencial obtenido de la muestra B. salicifolius del Cerrado difiere de la obtenida a partir de muestras recogidas en los biomas del sur de América del Sur.
Subject(s)
Oils, Volatile/chemistry , Plant Leaves/chemistry , Myrtaceae/chemistry , Sesquiterpenes/analysis , Gas Chromatography-Mass SpectrometryABSTRACT
Blepharocalyx salicifolius (Kunth) O. Berg, Myrtaceae, is an endemic species that occurs at Southern America. This species was studied to intend to isolation of the active compounds that could be used in vitro model against leishmaniosis, tumoral cell and paracoccidioidomycosis. After Gel Permeation Chromatography, the ethanolic extract from leaves yielded sixteen fractions. Five compounds were isolated and assayed, showing activity against tumoral cells, from 3.33 to 12.83 µg.mL-1; Leishmania (Leishmania) amazonensis from 2.19 to 20.80 µg.mL-1 and Paracoccidioides brasiliensis from 3.10 to 12.5 µg.mL-1.
ABSTRACT
Crude ethanolic extracts from Blepharocalyx salicifolius (Kunth) O. Berg, Myrtaceae, was fractioned by Gel Permeation Chromatography, using SephadexTM LH-20 gel. Sixteen fractions were obtained and were supplied to cytotoxicity in vitro assay against Leishmania (L.) amazonensis amastigota cells. It was observed eight cytotoxic fractions against Leishmania (L.) amazonensis amastigota cells at range of 19 to 29 µg.mL-1. Two of them were not citotoxic against human peripheral blood mononuclear cell, with a great potential to isolation of more selective leishmanicidal substances.
O extrato etanólico bruto de Blepharocalyx salicifolius (Kunth) O. Berg, Myrtaceae, foi fracionado por meio de Cromatografia de Permeação em Gel, utilizando-se SephadexTM LH-20. Dezesseis frações foram obtidas e foram submetidas ao ensaio de citotoxicidade in vitro contra células amastigotas de Leishmania (Leishmania) amazonensis. Verificou-se atividade citocida contra células amastigotas de Leishmania (L.) amazonensis em oito frações, a uma concentração de 19 a 29 µg.mL-1. Duas destas frações apresentaram baixa toxicidade para células mononucleares de sangue periférico humano, com grande potencial de isolamento de substâncias leishmanicidas mais seletivas.
ABSTRACT
Las plantas medicinales constituyen un recurso invalorable por su potencialidad farmacológica que hace necesario estudiarlas ante la demanda de nuevos fármacos, especialmente con actividad antifúngica. La flora de Entre Ríos (Argentina) posee numerosas especies con antecedentes etnobotánicos de uso como antisépticos en heridas. Los extractos de 6 especies vegetales seleccionadas fueron evaluadas en su actividad antifúngica mediante el método de microdilución en caldo, contra cepas tipificadas de Aspergillus niger y Trichophyton rubrum. Con excepción de Castela tweedii, las demás especies ensayadas, Baccharis articulata, Blepharocalyx salicifolius, Eichhornia azurea, Ludwigia peploides y Schinus fasciculatus, mostraron actividad in vitro en alguno de sus extractos, lo que auspicia la continuidad de los estudios.
Medicinal plants are a valuable resource due to its pharmacological potentiality ant its study is necessary in the face of the demand of new drugs specially those having antifungal activity. The Entre Ríos (Argentina) has many species with ethnobotanical backgrounds used as antiseptic agent for wounds. Extracts from 6 selected vegetal species were assessed for its antifungal activity by broth microdilution to typified strains of Aspergillus nigery Trichophyton rubrum. Except for Castela tweedii, the other assayed species, Baccharis articulate, Blepharocalyx salicifolius, Eichhornia azurea, Ludwigia peploides and Schinus fasciculatus showed in vitro activity in some extracts, being necessary a continuing study.