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AIM: To investigate the relationship between blood biochemical parameters and choroidal thickness(CT)in na&#xEF;ve-treatment of patients with nonproliferative diabetic retinopathy(NPDR).METHODS: Prospective cross-sectional study. A total of 92 patients(92 eyes)with na&#xEF;ve-treated NPDR were selected in the Affiliated Eye Hospital of Nanchang University from July 2021 to July 2022. All of the patients included in this study were subjected to ophthalmologic examination including enhanced depth imaging optical coherence tomography(EDI-OCT), best corrected visual acuity(BCVA), fundus fluorescence angiography, intraocular pressure, slit lamp and fundus examination. At the same time, they were also underwent hematological examination including blood glucose, glycosylated hemoglobin(HbA1c), serum creatinine, uric acid, urea, β2 microglobulin, high density lipoprotein(HDL), low density lipoprotein(LDL), serum calcium, serum potassium and other tests. According to the glomerular filtration rate(eGFR)and CT, the patients were divided into normal, mild and moderate to severe renal function group, pachychoroid group and the leptochoroid group. The blood biochemical indexes, CT differences and theirs correlation were analyzed in na&#xEF;ve-treated NPDR patients.RESULTS: A total of 92 cases(92 eyes)were included in this study, with 51 males(51 eyes), 41 females(41 eyes), 45 right eyes and 47 left eyes. There was no significant difference in age, axial length and disease duration among the three groups(all P>0.05). There were significant differences in nasal 0.5, 1.5mm, subfoveal and temporal 0.5, 1.5mm CT among three groups(all P<0.05). There was no statistical significance in age, axial length and the course of disease between the pachychoroid and leptochoroid groups(all P>0.05). There were significant differences in creatinine, eGFR, uric acid, urea, potassium and β2-microglobulin between the pachychoroid group and the leptochoroid group(all P<0.05). There were no significant differences in total cholesterol, triglyceride, HDL, LDL, HbA1c, serum calcium and glucose between the two groups(all P>0.05). CT was negatively correlated with creatinine, urea, uric acid and β2-microglobulin, and positively correlated with eGFR. Multiple regression analysis showed that the thicker the nasal 0.5mm CT, the higher the eGFR(B=0.292, P<0.001). CT affected creatinine, eGFR, urea, uric acid and β2-microglobulin(B=16.5, 64.6, 24.1, 18.1, 20.3; P=0.008, <0.001, <0.001, 0.004, 0.001), while there were no significant effects on total cholesterol, triglyceride, glucose, serum calcium, HDL and LDL(all P>0.05).CONCLUSION: In patients with na&#xEF;ve-treated NPDR, the thicker the CT, the better the renal function. The decrease of CT in newly diagnosed NPDR patients is an important hint to pay attention to the renal function.
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Objective:To analyze the clinical manifestations, blood biochemistry indexes, imaging and genetic characteristics of mitochondrial encephalomyopathy with lactic academia and stroke 1ike episodes (MELAS) in children, so as to provide the basis for the diagnosis of MELAS and reduce the misdiagnosis rate.Methods:The clinical data of children with MELAS admitted to the Second Affiliated Hospital of Wenzhou Medical University from January 2000 to December 2020 were retrospectively analyzed, and healthy children undergoing physical examination during the same period were selected as control group.The clinical data were compared between two groups, and the clinical manifestations, blood biochemistry indexes, electrocardiogram, cardiac ultrasound, cranial imaging and genetic testing were analyzed.Results:A total of eight children in MELAS group were collected, including three males and five females.The average age of onset was(9.90±3.89)years.There were eight children in control group, including four boys and four girls, with an average age of(7.92±2.51)years.Among the eight children with MELAS, there were six cases of vomiting, eight cases of epilepsy, five cases of headache, two cases of growth retardation, one case of mental retardation, one case of diabetes, and one case of peripheral neuropathy.The levels of lactate, lactate dehydrogenase, creatine kinase, and pyruvate in MELAS group were higher than those in control group, and the differences were statistically significant( P<0.05). Brain MRI abnormalities were observed in all patients, among which five patients had lesions located in the cerebral cortex, mostly in the parietal occipital temporal lobe, one patient had lesions located in the basal ganglia, and two patients had lesions in both cortex and basal ganglia.MRS of five cases showed inverted lactate peak with bimodal change.The electroencephalogram of eight cases showed slow wave of background activity, and epileptic discharge was observed in two cases.Seven children with MELAS had mtDNA locus mutation M. 3243A>G, and one patient had M. 8344A>G mutation.Eight cases were treated with symptomatic and supportive therapy, and were followed up for 3-5 years, most of them were hospitalized repeatedly because of similar chief complaints.The course of disease was prolonged and repeated, and the symptoms were relieved and discharged after about one week of hospitalization. Conclusion:The clinical manifestations of MELAS in children are diverse, and early diagnosis is difficult.Blood biochemistry, imaging characteristics and genetic testing results are helpful for early diagnosis, early treatment and delaying the progression of the disease.
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OBJECTIVE: To establish the laboratory historical control values for biological indicators in SD rats with 28-day repeated dose oral toxicity tests. METHODS: The body mass, blood routine indexes, serum biochemical indexes, organ mass and organ coefficient of 10 batches of specific pathogen free SD rats in the control group and the control additional group were collected for 28-day repeated dose oral toxicity tests, and the historical control values was established. RESULTS: The body mass of both male and female SD rats increased with the increasing age(all P<0.01). The body mass of male rats was higher than that of female rats each week(all P<0.01). The body mass, blood routine and serum biochemical indexes, organ mass and organ coefficient of SD rats were affected by the age and gender of rats to varying degrees. The effects of age and gender on organ mass and organ coefficient were not consistent. The laboratory historical control values of body mass, blood routine indexes, serum biochemical indexes, organ mass and organ coefficient of SD rats were established according to the age measured in weeks and the gender of rats. CONCLUSION: The laboratory control values of biological indicators of SD rats should be established according to different weekly age and the gender of rats. Organ coefficient is more suitable as an observation index for toxicological safety evaluation compared with organ mass.
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Objective To investigate and analyze the characteristics of blood physiological and biochemical parameters of Tibetan chickens bred in Guangzhou. Methods Blood samples of Tibetan chickens bred in Guangzhou were collected,and the physiological and biochemical parameters were measured. Results (1)The blood RBC,PLT,PDW, RDW-SD and P-LCR were not significantly different in the males than females(P > 0.05).(2)HCT(P < 0.05), MCHC(P< 0.05),MPV(P< 0.05),HGB(P< 0.01),MCV(P< 0.01)and MCH(P< 0.01)were significantly higher between the males and females.(3)RDW-CV was significantly lower in the blood physiological parameters of males than females.(4)AST,TRIG,ALKP,ALT,Ca,CHOL,CREA,GLU,PHOS and TBIL were not remarkably different in the blood of males than females(P > 0.05).(5)The blood AMYL(P < 0.05)and TP(P < 0.01)were significantly higher in the males than females.(6)The blood ALB(P< 0.01),UREA(P< 0.05), and GLOB(P<0.01)were significantly lower in the males than females. Conclusions The essential data of blood physiological and biochemical indexes of Tibetan chickens bred in Guangzhou are obtained.
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OBJECTIVES: This study examined the effects of nutrition education and exercise therapies on the hematological status and diabetes knowledge of diabetic patients. For this purpose, a 12-week intensive management program was provided to diabetic patients participating in an exercise program in S health subcenter in Kwangju city and the effects were analyzed. METHODS: The subjects were 26 diabetic patients, who provided written informed consent. As a preliminary survey, this study examined the general characteristics, physical status, obesity, blood pressure, hematological status, daily activity level, diabetes knowledge, diet performance, and barriers to diet therapy. After the 12-week intensive management program was completed, a post-test was conducted in the same way as the preliminary test. The data were analyzed with using SPSS 18.0. The data from this study are presented as the mean ± standard deviation. A paired t-test was conducted to compare differences in the means before and after the program. Statistical significance was set to p < 0.05. RESULTS: The results of the program are presented as follows. The HDL-cholesterol levels changed from 39.8 ± 10.5 mg/dL to 48.3 ± 13.1 mg/dL, showing a significant increase (p < 0.001). The blood sugar 2 hours after a meal changed from 175.2 ± 67.1 mg/dL to 140.5 ± 42.5 mg/dL, showing a significant decrease (p=0.014). The glycosylated hemoglobin levels decreased significantly from 6.7 ± 1.1% to 6.3 ± 1.0% (p=0.010). The total scores of the daily activity levels increased significantly from 3.8 ± 2.4 to 4.8 ± 2.5 (p=0.040). The scores of knowledge on diabetes increased from 11.5 ± 3.6 to 14.0 ± 3.8 (p=0.001). The scores of knowledge on diet therapy changed from 6.7 ± 2.2 to 7.9 ± 1.7, showing a significant increase (p=0.027). CONCLUSIONS: The 12-week intensive management program intervened by nutrition education and exercise therapies induced positive changes to the HDL-cholesterol, blood sugar 2 hours after a meal, glycosylated hemoglobin, daily activity levels, and knowledge on diabetes.
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Humans , Blood Glucose , Blood Pressure , Diet , Diet Therapy , Education , Exercise Therapy , Glycated Hemoglobin , Informed Consent , Meals , ObesityABSTRACT
OBJECTIVES: This study examined the effects of nutrition education and exercise therapies on the hematological status and diabetes knowledge of diabetic patients. For this purpose, a 12-week intensive management program was provided to diabetic patients participating in an exercise program in S health subcenter in Kwangju city and the effects were analyzed. METHODS: The subjects were 26 diabetic patients, who provided written informed consent. As a preliminary survey, this study examined the general characteristics, physical status, obesity, blood pressure, hematological status, daily activity level, diabetes knowledge, diet performance, and barriers to diet therapy. After the 12-week intensive management program was completed, a post-test was conducted in the same way as the preliminary test. The data were analyzed with using SPSS 18.0. The data from this study are presented as the mean ± standard deviation. A paired t-test was conducted to compare differences in the means before and after the program. Statistical significance was set to p < 0.05. RESULTS: The results of the program are presented as follows. The HDL-cholesterol levels changed from 39.8 ± 10.5 mg/dL to 48.3 ± 13.1 mg/dL, showing a significant increase (p < 0.001). The blood sugar 2 hours after a meal changed from 175.2 ± 67.1 mg/dL to 140.5 ± 42.5 mg/dL, showing a significant decrease (p=0.014). The glycosylated hemoglobin levels decreased significantly from 6.7 ± 1.1% to 6.3 ± 1.0% (p=0.010). The total scores of the daily activity levels increased significantly from 3.8 ± 2.4 to 4.8 ± 2.5 (p=0.040). The scores of knowledge on diabetes increased from 11.5 ± 3.6 to 14.0 ± 3.8 (p=0.001). The scores of knowledge on diet therapy changed from 6.7 ± 2.2 to 7.9 ± 1.7, showing a significant increase (p=0.027). CONCLUSIONS: The 12-week intensive management program intervened by nutrition education and exercise therapies induced positive changes to the HDL-cholesterol, blood sugar 2 hours after a meal, glycosylated hemoglobin, daily activity levels, and knowledge on diabetes.
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Humans , Blood Glucose , Blood Pressure , Diet , Diet Therapy , Education , Exercise Therapy , Glycated Hemoglobin , Informed Consent , Meals , ObesityABSTRACT
Objective To observe the effect of different storage time on 14 blood biochemical indexes in rats. Methods Randomly selected 40 adult SD rats were included in this study. Fasting venous blood samples were collected, serum was separated, sealed, and stored in the refrigerator (4℃ and -20℃). The serum parameters were detected at 0 h,4 h,24 h,96 h and 7 d, respectively, using an automatic biochemical analyzer. A total of 14 blood biochemical indexes were detected, including alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) , alkaline phosphatase (ALP), total protein (TP), albumin (ALB), creatinine (CREA-J), uric acid (UA), urea nitrogen (UREA), blood glucose ( GLU) , total cholesterol ( TC) , triglyceride ( TG) , low density lipoprotein cholesterol ( LDL-C) , creatine kinase ( CK) and lactate dehydrogenase ( LDH) . The effects of serum storage time on blood biochemical results were compared. Results The trends of blood biochemical data in male and female rats were consistent. C ompared with the indexes of serum preserved at 4℃ for 0 h, the ALP was significantly reduced after storage for 4 h, 24 h, 96 h, and 7 d (P< 0. 05), ALB were significantly increased after 96 h and 7 d (P< 0. 01), CREA-J was significantly increased after 96 h, 7 d (P<0. 05), UA was significantly increased after 24 h, 9 h, and 7 d (P < 0. 01), and no significant changes in other indicators ( P> 0. 05 ) . Compared with the values of 0 h serum, the serum preserved at -20℃ showed that ALT was significantly increased after 7 d (P < 0. 01), AST significantly increased after 96 h and 7 d (P< 0. 05), TP significantly decreased after 4 h and 24 h ( P< 0. 05 ) , ALB significantly increased after 4 h, 24 h, 96 h, and 7 d ( P< 0. 01 ) , CREA-J significantly increased after 24 h, 96 h, and 7 d (P< 0. 01), UA significantly increased after 4 h, 24 h, 96 h, and 7 d (P< 0. 01), TC significantly increased after 4 h, 24 h, 96 h, and 7 d (P< 0. 01), TG significantly increased after 96 h and 7 d (P< 0. 05), CK significantly increased after 96 h and 7 d (P< 0. 05), LDH significantly increased after 96 h and 7 d ( P < 0. 05 ) , and no significant changes in other indicators ( P > 0. 05 ) . Conclusions The biochemical tests of rat serum should be immediately performed as they were collected, especially for ALP test. For the sera stored at 4℃, the test should be finished in different times:UA test in 4 hours, ALB and CREA-J test in 24 hours, and ALT, AST, TP, UREA, GLU, TC, TG, LDL-C, CK, and LDH test in 7 days.
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ABSTRACT Objetive . Evaluation of hematological and biochemical parameters of culture channel catfish (Ictalurus punctatus) under acute stress by management and capture practice. Materials and methods . Fish (200 g mean) were maintained in culture tanks and divided in two treatments, in duplicate, (n=15x2x2=60 fishes). Thirty catfish were exposed for 5 min to acute stress (TE) by management and capture practice, while other group not (control group, TnE). 10 fish for treatment were collected at 0, 6, and 24 h post-stress for blood collection, where TnE fishes were anesthetized along work. Complete blood count (manual method) and blood biochemical (spectrophotometry) of fish samples were evaluated and their results were analyzed using a Student's t-distribution. Results . The erythrocytes, hematocrit, hemoglobin and glucose level of TE animals was significantly higher (p<0.05) at 6 h post-stress, in comparison of TnE. Immune cells in fish TE decreased at 6 and 24 h post-stress, where leukocytes and lymphocytes were significantly lower that TnE (p<0.05) at 24 h post-stress. Other evaluated parameters did not show significant differences along this study. Conclusions . Those results suggest that several hematological and blood biochemical parameters in fish changed by acute stress generated by management and capture practice.
RESUMEN Objetivo . Evaluar los efectos del estrés agudo debido al manejo y captura sobre los parámetros hematológicos y bioquímicos en bagre de canal (Ictalurus punctatus) bajo cultivo. Materiales y métodos . Los peces (200 g promedio) fueron mantenidos en tanques de cultivo y divididos en dos tratamientos, por duplicado, (n= 15 x 2 x 2 = 60 peces). Treinta bagres fueron expuestos por 5 min a estrés agudo (TE) por manejo y captura, mientras que otro grupo no (grupo control, TnE). Diez peces de cada tratamiento fueron colectados a las 0, 6, y 24 h post-estrés para la extracción de sangre, los bagres del TnE fueron anestesiados durante su manejo y captura. Se evaluó el hemograma (método manual) y bioquímica sanguínea (espectrofotometría). Los resultados fueron analizados mediante la prueba de t student. Resultados . El contenido de eritrocitos, hematocrito, hemoglobina y glucosa de los animales TE fue significativamente mayor (p<0.05) a las 6 h post-estrés en comparación de TnE. Las células inmune en peces TE disminuyeron a las 6 y 24 h post-estrés, siendo leucocitos y linfocitos significativamente menores en el TnE (p<0.05) a las 24 h post-estrés. Otros parámetros evaluados no presentaron diferencias significativas en lo largo del estudio. Conclusiones . Los resultados sugieren que varios indicadores hematológicos y bioquímica sanguínea en los peces son alterados por el estrés agudo ocasionado por manejo y captura.
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Catfishes , Biochemistry , Blood Cell CountABSTRACT
Objective To investigate the correlation of the polymorphisms of angiotensin Ⅱtype 1 receptor (AT1R)gene A1166C and angiotensinogen(AGT)gene M235T and blood biochemical indicators with essential hypertension(EH)in the male of Yi nationality in Yunnan province. Methods 92 hypertensive and 70 normotensive of Yi people were recruited in Yunnan province. Their basic information and blood biochemical indicators were obtained by questionnaire and standardized physical examination. The genes polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)technique. Results(1)For the male EH patients of Yi nationality,1166C and 235T allele frequencies were 0.038 and 0.772. No significant differences in allele frequency or genotype frequency were observed between the two groups(P > 0.05).(2)The levels of TC,LDL-C and TG in EH were significantly higher than those in controls(P < 0.05). Conclusion The AT1R A1166C and AGT M235T polymorphisms were not related to the pathogenesis of EH in the male of Yi people. However,the high levels of TC,TG and LDL-C might be the risk factors of EH.
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Objective Beagle dogs are commonly used animal for drug safety evaluation .As the necessary parameters , blood biochemical indicators are detected in acute or chronic toxicity tests .This study aims at assessing the influence of different preservation conditions and different preservation time on blood biochemical indicators to ensure the reliability of test results of long-term toxicity assessment .Methods Six Beagle dogs (3 males and 3 females) were used in this study .After collection and preparation of serum samples , biochemical indicators were detected after preservation in refrigerator at 2-8℃for 1, 2, 5, 8, and 12 hours;after preservation in ice transportation boxes at 2-10℃for 2, 5, and 8 hours;and after preservation in refrigerator at -20℃ for 1, 3, and 5 days.The biochemical indicators included alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) and alkaline phosphatase ( ALP ) , total protein ( TP ) , albumin (propagated), urea, creatinine (CREA), glucose (GLU), total cholesterol (TCHO), total bilirubin (TBIL), creatine kinase ( CK ) , gamma pancreatic acyl transferase ( GGT ) , calcium ( CA ) , lactate dehydrogenase ( LDH ) , phosphorus ( P) , high-density lipoprotein cholesterol ( HDL-C) , low density lipoprotein cholesterol ( LDL-C) , triglyceride ( TG) , sodium ( Na+) , potassium ( K+) and chloride ( Cl -) .Results Compared with the results of samples preserved for 1 hour, the LDL-C result of that preserved in refrigerator at -20℃for 5 days was significantly increased (P0.05 ) , and the coefficient of variation of LDH was 41%.Conclusions According to the test results of blood biochemical indicators in the Beagle dogs detected after different preservation conditions and different preservation time in this study , detection test should be done within 1 hour, if not, detection should be done within 12 hours for the samples preserved at 2~8℃, or within 3 days for the sample preserved at -20℃.For transportation of serum samples , the serum samples should be placed in the ice box at 2~10℃, and detection test should be done within 8 hours .
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Objective To analyze the growth phenotype and blood biochemical parameters of chromosome 1 substi-tution mouse strain(CSS1), and investigate their potential of QTL mapping .Methods Body weight, body length, tail length, organ weight of the CCS1 mice were measured at different days to create a growth curve while blood biochemical in -dexes were measured at about the 80th day.Results The CCS1 mice were different from C57BL/6 mice in several inde-xes.Compared with the C57BL/6 mice during different developmental stages , six strains including B6-Chr1KM mice were significantly different in body weight .There were five strains including B6-Chr1CM mice significantly different with C57BL/6 mice in body length, and all of the CSS1 mice were significantly different from C57BL/6 mice in tail length.Part of CCS1 mice were significantly different from C57BL/6 mice in the weight of liver, spleen, kidney and brain.The ALT of female B6-Chr1CM mice was significantly higher than that in the C 57BL/6 mice.The ALP of female B6-Chr1HZ mice was signifi-cantly higher than that in the male C57BL/6 and B6-Chr1KM mice, and was significantly lower than that in the C57BL/6 mice.The TB of male B6-Chr1CM, B6-Chr1SMX and B6-Chr1HZ mice was significantly higher than that of the C 57BL/6 mice.The TG of male B6-Chr1SMX mice and male B6-Chr1TW mice was significantly higher than that in the C 57BL/6 mice. Conclusions The phenotype of Chr1 CSS mice is quite different from commonly used inbred strain C 57BL/6 mice.CCS1 mice show great potential in QTL mapping for their characteristic growth phenotype and blood biochemical indexes .
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Objective To detect the blood parameter, blood biochemical and electrolyte indices of the F1 generation of Rongshui miniature pig ( RMP) .Methods The blood of 43 female and 42 male RMPs of 4 th month old, and 36 RMPs of 12th month old ( half male and female) were extracted from jugular vein.And the blood parameter, blood biochemical and electrolyte indices were detected by blood analyzer and automatic biochemical analyzer.Results In the same month-old RMP, no significant difference between male and female were found in most indices of blood parameter, blood biochemical and electrolyte indices.On the other hand, many indices were difference between 4th month old and 12th month old RMPs of same gender.Compared with the 4th month old RMP, the 12th month old RMP decreased significantly in WBC and PLT, increased in HGB ( P 0.05 ) .Serum ALT, AST, ALP, CK (male), LDH(male), A/G, BUN, GLU (female), CHOL (male) and K+decreased significantly (P <0.05), while serum TP, TBIL, CR and Ca2+increased significantly (P <0.05),but serum CHOL, TG, HDL-C and LDL-C were not different.86.4%(19/22) biochemical and electrolyte indices in RMP were in/or close to the range of normal value of human.Conclusion Most of the blood parameter, blood biochemical and electrolyte indices of RMP were close to human’ s normal value.
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Objective To explore the effect of different concentrations of hypertonic saline for cerebral ede -ma on blood biochemical and osmotic pressure .Methods 60 cases were randomly divided into the 3% HS group, 7.5%HS group and the mannitol group .The patients were randomly assigned to infusion of 3% HS, 7.5% HS or 20%mannitol (all 2 mL/kg).Blood biochemical and osmotic pressure on patients were measured before and after the treatment among groups .Results Serum Na +, Cl -and osmotic pressure were significantly higher at 2 hours and 22 hours of reperfusion than at baseline (p<0.05) in the 7.5% HS group and the mannitol group .The difference were significant on serum Na +, Cl-and osmotic pressure at 2 hours and 22 hours of reperfusion in the 7.5% HS group and the mannitol group than in the 3% HS group(p<0.05).Conclusion 3% hypertonic saline had less effects on blood biochemical and osmotic pressure than other groups for patients of cerebral edema .It is worthy of popularization and application .
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BACKGROUND: Unleaded petrol contains significant amounts of monocyclic aromatic hydrocarbons such as benzene, toluene, and xylenes (BTX). Toxic responses following occupational exposure to unleaded petrol have been evaluated only in limited studies. The main purpose of this study was to ascertain whether (or not) exposure to unleaded petrol, under normal working conditions, is associated with any hepatotoxic or nephrotoxic response. METHODS: This was a cross-sectional study in which 200 employees of Shiraz petrol stations with current exposure to unleaded petrol, as well as 200 unexposed employees, were investigated. Atmospheric concentrations of BTX were measured using standard methods. Additionally, urine and fasting blood samples were taken from individuals for urinalysis and routine biochemical tests of kidney and liver function. RESULTS: The geometric means of airborne concentrations of BTX were found to be 0.8 mg m-3, 1.4 mg m-3, and 2.8 mg m-3, respectively. Additionally, means of direct bilirubin, alanine aminotransferase, aspartate aminotransferase, blood urea and plasma creatinine were significantly higher in exposed individuals than in unexposed employees. Conversely, serum albumin, total protein, and serum concentrations of calcium and sodium were significantly lower in petrol station workers than in their unexposed counterparts. CONCLUSION: The average exposure of petrol station workers to BTX did not exceed the current threshold limit values (TLVs) for these chemicals. However, evidence of subtle, subclinical and prepathologic early liver and kidney dysfunction was evident in exposed individuals.
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Alanine Transaminase , Aspartate Aminotransferases , Benzene , Bilirubin , Calcium , Creatinine , Cross-Sectional Studies , Fasting , Hydrocarbons, Aromatic , Kidney , Liver , Occupational Exposure , Plasma , Serum Albumin , Sodium , Threshold Limit Values , Toluene , Urea , Urinalysis , XylenesABSTRACT
Objective To measure the range of routine blood counts and blood biochemical parameters ,to analyze hemorheology of the rats with congenital cataract .Methods Blood samples were taken from 90 rats with congenital cataract weight about 185 ~211 g.Routine blood analysis was performed and blood biochemical and hemorheology paramenters were determind using an automatic blood biochemical and hemorheology analyzer .Results There were no significant difference ( P >0.05 ) between the cataract rats and the normal rats in Blood test results; but there were significant difference between the microphthalmos cataract rats and the normal same -sex rats ( P <0.01 or P <0.05 ) . The biochemical results is the cataract rats and the normal rats were different significantly in ALB group ( P <0.01 or P<0.05), and the female microphthalmos cataract rats compared with the control rats had significant difference in Ure (P<0.01) , the female cataract rats ompared with the normal rats were very significant difference in Cr group ( P <0.05 or P <0.01).The erythrocyte counts of the male cataract rats and male microphthalmos cataract rats were significantly lower than that in the female ones, respectively(P <0.05, P <0.01).The platelet counts of the male cataract rats and the male microphthalmos cataract rats were significantly higher than that in the female ones , respectively(P <0.01), and the creatinine of the male cataract rats and the male microphthalmos cataract rats were significantly lower then that in the female ones, respectively(P <0.01).There were no significant difference in every group on hemorheology .Conclusions There were significant differences in some blood indexes between the congenital cataract rats and the normal rats .These data may become useful reference for biomedical researcher in this field .
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O objetivo deste trabalho foi avaliar o efeito de duas dietas: baixo e alto nível de suplementação, fornecidas a vacas em estádio avançado de lactação sobre os aspectos produtivos, as características físico-químicas do leite e o perfil bioquímico sanguíneo. Foram utilizadas 12 vacas da raça Holandês, com média de 514kg de peso corporal, escore de condição corporal 3,5, produção de leite de 13 litros vaca-1 dia-1, com média de 349 dias de lactação. Os tratamentos foram: baixo e alto nível de suplementação para vacas mantidas em campo natural. Foram avaliados o peso vivo, o escore de condição corporal, a produção leiteira individual, as características físico-químicas e a contagem de células somáticas do leite (CCS), além do perfil bioquímico do sangue. As vacas, mesmo em estádio lactacional avançado, responderam ao aumento do aporte nutricional, com incrementos de ganho de peso, ganho de condição corporal e de produção de leite e produção leiteira corrigida para gordura, além de maiores teores de gordura e de sólidos totais. As demais características do leite e do sangue não foram alteradas. O aumento do aporte nutricional de vacas lactantes com estádio de lactação avançado pode ser uma opção viável para alcançar maior nível de produção leiteira e melhores condições físicas das vacas em sistemas de produção baseadas na utilização de pastagem, sem afetar adversamente a concentração dos componentes lácteos.
The trial aimed to verify the effects of two feeding supplements: low and high level supplementation given to cows at an extended lactation stage upon productive aspects, physical-chemical composition of milk and blood biochemical profile. Twelve lactating Holstein cows, with 514kg of body weight, body condition score of 3.5, yielding 13L milk cow-1 day-1 and approximately 349 days of lactation were used. Treatments were low or high level of supplementation for lactating cows grazing natural range pasture. Cow's body weight and body condition score, individual milk yield, physical and chemical characteristics and somatic cells count, besides biochemical blood profile were evaluated. Increased nutrient supply augmented body weight gain, body condition score, fat corrected milk, milk fat and total solids contents, but did not change the others milk components nor blood biochemical profile. Increasing nutrient supply for cows at an extended lactation might be a valuable option to match milk productivity and improved physical aspects of dairy cows kept on grazing systems, without adverse effects upon milk components yield.
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Background: Metabolic syndrome is characterized by a group of metabolic risk factors in one person but not a real disease. For this reason, metabolic syndrome is underestimated by clinicians and individuals. Metabolic syndrome that was not detected and treated timely can cause serious diseases. Objectives: 1) To investigate the abnormal blood biochemical parameters related to metabolic syndrome. 2) To determine the abnormal ratio of these parameters. 3) To screen the metabolic syndrome among the staffs of Ha Noi Medical University. Subjects and method: 229 staffs underwent laboratory tests to diagnose for the metabolic syndrome. Abnormal blood biochemical parameters were defined as follows: Fasting blood glucose level increased 6.1mmol/l or more. Triglycerides level is 2.3mmol/l or more, HDL-cholesterol decreased to less than 1.03mmol/l for men and less than 1.24mmol/l for women. Total cholesterol level is 5.2mmol/l or more. CT to HDL-C ratio is more than 3.63 and LDL cholesterol level is 3.5mmol/l or more. Results. Rate of subjects with glucose intolerance or blood fasting glucose higher than 6.1mmol/L is 20.09%. Rate of subjects with dyslipidemia is 61.14%. Average levels of blood glucose, triglycerides and cholesterol increase with age. Number of staffs of Ha Noi Medical University have metabolic syndrome in 2007 are 32 persons (14%). M/F rate is equal and most common in persons over 45 years of age. Conclusion:There are significant number of staffs at the Ha Noi Medical University that had abnormal blood glucose and cholesterol levels related to metabolic syndrome.
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Metabolic SyndromeABSTRACT
Objective To study the effect of the low free radical cigarettes (test cigarettes) on the blood biochemical parameters,lung and polychromatophilic red cell micronucleus in rats. Methods 36 SD rats were randomly divided into the blank control group,the comparison smoke group and the experimental smoke group (low free radical cigarettes),12 in each. Let the rats smoke passively for 30 day,daily 12 cigarettes,then the determination of blood biochemical parameters and the polychromatophilic red cell micronucleus rate in the bone marrow,the pathological examination of the trachea and the lung tissues were conducted. Results Compared with the blank control,no significant changes were seen in the blood biochemical parameters and the polychromatophilic red cell micronucleus rate. The pathological examination of the lung tissues indicated that the pathological changes in the experimental smoke group were slight compared with the comparison smoke group. Conclusion No seriously adverse effects of low free radical cigarettes on the lung tissues of rats have been seen in the present study,that indicates that maybe the low free radical cigarette has an advantage in the health safety.
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Background: Massive blood transfusion is common in surgery: trauma, complicated surgery, organ implantation. Stored blood contains much lactate, ammonia, products of metabolism. These products have negative effects on patient\u2019s metabolic function which may be a cause of death. Objective: To study the change of blood biochemical indexes in surgical patients with massive blood transfusion at Viet Duc hospital. Subjects and method: A prospective study was conducted in the adult patients who had emergency and operation at Viet Duc hospital, from December/2004 to August/2006. All of them were transfused over 3000 ml of blood within 24 hours (pre-operation, intra-operation and post-operation). Results: During the period of study, there were 70 surgical patients with massive blood transfusion: men were more than woman (64%) and the mean age: 38\xb117. The survival rate was significantly improved (57%). The average transfused blood per patient per 24 hours was 3995ml\xb11067ml. There was a decrease in the value of pH, acid-base and HCO3 (the average pH value 7.218\xb10.181, lowest value 6.7). The average value of pH and HCO3\xac was higher in the survival patients than died patients (p<0.05). The mean level of K+ was 3.8\xb10.96mml/l. The increase in the level of K+ had a positive association with the amount of transfused blood. The mean level of Ca++ \xac\xacdecreased under the normal level (0.98mml). Conclusion: It should monitor blood gas and electrolytes continuously in surgical patients with massive blood transfusion in order to identify disorders soon for timely treatment.
Subject(s)
Blood TransfusionABSTRACT
Objective To determine the relationship between blood biochemical indexes and severity of NAFLD by establishing various models of NAFLD with different degrees in rabbits. Methods Thirty-two healthy rabbits were randomly divided into normal control (n=8) and three experimental groups(n=24). Fatty liver animal models were established by giving high fat,high sugar diet. The serum TG,TC,AST,ALT and pathological evaluation were detected respectively after 4,6 and 8 weeks. Results The rabbits developed hyperlipidemia and slight fatty liver after four weeks. Along with the worsening of fat liver,the serum TG level kept rising.Conclusion The blood biochemical indexes can serve as a reliable technique for diagnosing fatty liver disease in clinical.